Browse > Article
http://dx.doi.org/10.47853/FAS.2022.e2

Characterization of ovarian culture in vitro and sex steroids in vivo by recombinant eel gonadotropin treatments in the eel Anguilla japonica  

Kim, Dae-Geun (Gyeongsangnam-do Fisheries Resources Research Institute)
Kim, Jung-Hyun (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Baek, Hea-Ja (Department of Marine Biology, Pukyong National University)
Kim, Shin-Kwon (Department of Aquaculture Research, National Institute of Fisheries Science)
Min, Kwan-Sik (Animal Biotechnology, Graduate School of Future Convergence Technology, School of Animal Life Convergence Science, Institute of Genetic Engineering, Hankyong National University)
Kim, Dae-Jung (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Publication Information
Fisheries and Aquatic Sciences / v.25, no.1, 2022 , pp. 12-19 More about this Journal
Abstract
In the present study, we investigated the effects of recombinant eel gonadotropins (rec-GTHs) on maturation induction in immature ovarian culture in vitro and sex steroid hormones in vivo in the Japanese eel Anguilla japonica. To study the in vitro effects of rec-GTHs on estradiol-17β (E2) production in immature ovarian tissues, ovarian tissues were incubated with different doses of rec-follicle-stimulating hormone (rec-FSH) or rec-luteinizing hormone (rec-LH). The results revealed that the E2 levels in the rec-FSH (0.1, 0.5, or 1 ㎍/mL)- and rec-LH (0.1 or 0.5 ㎍/mL)-treated groups were significantly higher than those in the female eels from the control group. Furthermore, to investigate the in vivo effects of rec-GTHs on the gonadosomatic index (GSI) and plasma sex steroid hormone levels, the eels were injected intraperitoneally with eel's ringer (control), salmon pituitary extract (SPE; for female eels), human chorionic gonadotropin (hCG; for male eels), rec-FSH, rec-LH, and rec-FSH + rec-LH once a week. The results revealed that except for the SPE and the hCG groups, none of the groups exhibited a significant difference in GSI values. However, in vivo plasma E2 levels increased at the end of 4 weeks after rec-FSH treatment in female eels. Based on these results, it is suggested that rec-GTHs may have a positive effect on sexual maturation in female eels; however, further studies on complementary rec-protein production systems and additional glycosylation of rec-hormones are needed to elucidate hormone bioactivity in vivo and in vitro.
Keywords
Eel Anguilla japonica; Gonadotropin; Sexual maturation; Recombinant hormone;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Yaron Z, Gur G, Melamed P, Rosenfeld H, Elizur A, Levavi-Sivan B. Regulation of fish gonadotropins. Int Rev Cytol. 2003;225:131-85.   DOI
2 Kazeto Y, Kohara M, Miura T, Miura C, Yamaguchi S, Trant JM, et al. Japanese eel follicle-stimulating hormone (FSH) and luteinizing hormone (LH): production of biologically active recombinant FSH and LH by Drosophila S2 cells and their differential actions on the reproductive biology. Biol Reprod. 2008;79:938-46.   DOI
3 Moles G, Zanuy S, Munoz I, Crespo B, Martinez I, Mananos E, et al. Receptor specificity and functional comparison of recombinant sea bass (Dicentrarchus labrax) gonadotropins (FSH and LH) produced in different host systems. Biol Reprod. 2011;84:1171-81.   DOI
4 Nagahama Y. Endocrine regulation of gametogenesis in fish. Int J Dev Biol. 1994;38:217-29.
5 Ohta H, Kagawa H, Tanaka H, Okuzawa K, Iinuma N, Hirose K. Artificial induction of maturation and fertilization in the Japanese eel, Anguilla japonica. Fish Physiol Biochem. 1997;17:163-9.   DOI
6 Shin DH. Biochemical and histological studies on egg quality in the Korean eel (Anguilla japonica) [Ph.D. dissertation]. Sapporo, Japan: Hokkaido University; 2004.
7 Vischer HF, Granneman JC, Linskens MH, Schulz RW, Bogerd J. Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor. J Mol Endocrinol. 2003;31:133-40.   DOI
8 Kim DJ, Park CW, Kim DW, Park HK, Byambaragchaa M, Lee NS, et al. Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica. Gen Comp Endocrinol. 2016;233:8-15.   DOI
9 Legardinier S, Duonor-Cerutti M, Devauchelle G, Combarnous Y, Cahoreau C. Biological activities of recombinant equine luteinizing hormone/chorionic gonadotropin (eLH/CG) expressed in Sf9 and Mimic insect cell lines. J Mol Endocrinol. 2005;34:47-60.   DOI
10 Yamamoto K, Yamauchi K. Sexual maturation of Japanese eel and production of eel larvae in the aquarium. Nature. 1974;251:220-2.   DOI
11 Kim HW, Kim JH, Kim DG, Jung MH, Ji SC, Yang SG, et al. Effect of artificially sexual maturation by hCG dose and injection interval in cultured male eel, Anguilla japonica. J Fish Mar Sci Educ. 2018;30:1578-86.   DOI
12 Ko H, Park WD, Kim DJ, Kobayashi M, Sohn YC. Biological activities of recombinant Manchurian trout FSH and LH: their receptor specificity, steroidogenic and vitellogenic potencies. J Mol Endocrinol. 2007;38:99-111.   DOI
13 Kobayashi M, Hayakawa Y, Park W, Banba A, Yoshizaki G, Kumamaru K, et al. Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larvae. Gen Comp Endocrinol. 2010;167:379-86.   DOI
14 Kim DJ, Cho YC, Sohn YC. Molecular characterization of rockfish (Sebastes schlegeli) gonadotropin subunits and their mRNA expression profiles during oogenesis. Gen Comp Endocrinol. 2005;141:282-90.   DOI
15 Kagawa H, Iinuma N, Tanaka H, Ohta H, Okuzawa K. Effects of rearing period in seawater on induced maturation in female Japanese eel Anguilla japonica. Fish Sci. 1998;64:77-82.   DOI
16 Miura T, Yamauchi K, Nagahama Y, Takahashi H. Induction of spermatogenesis in male Japanese eel, Anguilla japonica, by a single injection of human chorionic gonadotropin (Endocrinology). Zool Sci. 1991;8:63-73.
17 Min KS, Hattori N, Aikawa JI, Shiota K, Ogawa T. Site-directed mutagenesis of recombinant equine chorionic gonadotropin/luteinizing hormone: differential role of oligosaccharides in luteinizing hormone- and follicle-stimulating hormone-like activities. Endocr J. 1996;43:585-93.   DOI
18 Combarnous Y. Molecular basis of the specificity of binding of glycoprotein hormones to their receptors. Endocr Rev. 1992;13:670-91.   DOI
19 Kim DJ, Park WD, Son YC, Bae JY, Yun SJ, Son MH, et al. Maturation induction by manchurian trout recombinant gonadotropin hormone (mt-rGTH) in female eel, Anguilla japonica. Dev Reprod. 2008;12:261-6.
20 Kasuto H, Levavi-Sivan B. Production of biologically active tethered tilapia LHβα by the methylotrophic yeast Pichia pastoris. Gen Comp Endocrinol. 2005;140:222-32.   DOI
21 Hamidoghli A, Bae J, Won S, Lee S, Kim DJ, Bai SC. A review on Japanese eel (Anguilla japonica) aquaculture, with special emphasis on nutrition. Rev Fish Sci & Aquacult. 2019;27:226-41.   DOI
22 Byambaragchaa M, Kim DJ, Kang MH, Min KS. Site specificity of eel luteinizing hormone N-linked oligosaccharides in signal transduction. Gen Comp Endocinol. 2018;268:50-6.   DOI
23 Choi E, Ko H, Shin J, Kim MA, Sohn YC. Expression of gonadotropin genes in manchurian trout Brachymystax lenok and production of recombinant gonadotropins. Fish Sci. 2005;71:1193-200.   DOI
24 Choi JH, Kim DJ, Hong SM, Jo SJ, Min KS, Sohn YC, et al. Molecular analysis and bioactivity of luteinizing hormone from Japanese eel Anguilla japonica, produced in silkworm pupae. Biotechnol Bioprocess Eng. 2016;21:381-8.   DOI
25 Ijiri S, Tsukamoto K, Chow S, Kurogi H, Adachi S, Tanaka H. Controlled reproduction in the Japanese eel (Anguilla japonica), past and present. Aquac Eur. 2011;36:13-7.
26 Kamei H, Ohira T, Yoshiura Y, Uchida N, Nagasawa H, Aida K. Expression of a biologically active recombinant follicle stimulating hormone of Japanese eel Anguilla japonica using methylotropic yeast, Pichia pastoris. Gen Comp Endocinol. 2003;134:244-54.   DOI
27 Kazeto Y, Tosaka R, Matsubara H, Ijiri S, Adachi S. Ovarian steroidogenesis and the role of sex steroid hormones on ovarian growth and maturation of the Japanese eel. J Steroid Biochem Mol Biol. 2011;127:149-54.   DOI
28 Kim JM, Munkhuu O, Byambaragchaa M, Lee BI, Kim SK, Kang MH, et al. Site-specific roles of N-linked oligosaccharides in recombinant eel follicle-stimulating hormone for secretion and signal transduction. Gen Comp Endocrinol. 2019;276:37-44.   DOI
29 Kim DJ, Kim HW, Kim JH, Lee BI, Hong SM, Min KS, et al. Induction of sexual maturation in female eels Anguilla japonica by recombinant eel gonadotropin produced in silkworm. Korean J Fish Aquat Sci. 2017;50:770-6.   DOI
30 Kim DJ, Bae JY, Kim EO. Changes in sex steroid hormones and ovarian development during artificial maturation of female eel, Anguilla japonica. Integr Biosci. 2007;11:117-24.   DOI
31 Ohta H, Sato Y, Imaizumi H, Kazeto Y. Changes in milt volume and sperm quality with time after an injection of recombinant Japanese eel luteinizing hormone in male Japanese eels. Aquaculture. 2017;479:150-4.   DOI
32 Okamura A, Yamada Y, Mikawa N, Horie N, Tanaka S, Tsukamoto K. Notochord deformities in reared Japanese eel Anguilla japonica larvae. Aquaculture. 2011;317:37-41.   DOI
33 Tanaka H, Kagawa H, Ohta H. Production of leptocephali of Japanese eel (Anguilla japonica) in captivity. Aquaculture. 2001;201:51-60.   DOI
34 Tanaka H. Progression in artificial seedling production of Japanese eel Anguilla japonica. Fish Sci. 2015;81:11-9.   DOI