• 한국약용작물학회지
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• 제12권5호
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• pp.378-383
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• 2004

Scopolamine and hyoscyamine are important anticholinergic compounds. To increase the productivity, we have selected various elicitors and developed culture system using a bubble column bioreactor (BCB). As the same manner of elicitation in flask cultures, the elicitors were introduced into BCB cultures and the productivity was investigated. Except the bacterial elicitor of Staphyllococcus aureus, the elicitors inhibited hyoscyamine production. In scopolamine production, the elicitors revealed different responses from the results obtained in flask cultures. The elicitors of KCl and Candida albicans less increased the production than flask cultures. However, methyl jasmonate and S. aureus showed stronger positive effects on tropane alkaloid production. In particular, S. aureus was the most effective elicitor on scopolamine production and the elicitor resulted in the highly increased production, approximately 10 times higher than the control culture.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권2호
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• pp.158-161
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• 2003

Root cultures of Angelica gigas Nakai were found to be sensitive to elicitation by poly-saccharide elicitors, such as methyl-$\beta$-cyclodextrin, glucan, carboxymethyl-$\beta$-chitin, chitosan, yeast extract and pectin. For the production of decursinol angelate, ca rboxymethyl-$\beta$-chitin and glucan were found to be the most efficient elicitors. The e nhanced accumulation of decursinol angelate was proportional to the increase of the phenylalanine ammonialyase (PAL) activity after the treatment with most of the elicitors. However, carboxymethyl-$\beta$-chitin treatment did not stimulate the PAL activity, despite the 1.6-fold increase in the decursinol angelate production.

• KSBB Journal
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• 제8권4호
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• pp.390-396
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• 1993

Thalicrtrum rugosum 식물세포 현탁배양에 의한 berberine 생산에 미치는 여러 가지 종류의 elicitor들의 영향에 관해 연구 하였다. 효모 유래의 elicitor, 15가지의 서로 다른 종류의 무생물 종류 elicitor, 3가지 곰팡이로부터 얻은 elicitor 들을 처리하여 세포의 생장 및 berberine 생산에 미치는 영향을 비교 분석해 본 결과, 이들 elicitor들은 T. rugosum에 의한 berberine 생산을 크게 증대시키지 못함을 확인하였다.

• 한국식품과학회지
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• 제34권1호
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• pp.8-12
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• 2002

수목에 스트레스를 가하여 정유를 증수할 목적으로 화학적 스트레스 자극제 5종과 생물적 스트레스 자극제 4종을 선발하여 실험을 실시하였다. 우선 정상적인 수목의 정유함량은 편백 2% 전후, 화백 1.6%, 소나무 0.4%, 잣나무 0.7%였으며, 상기 수종들은 모두 7월에 최대의 정유함량을 기록하였다. 또한 9종의 스트레스 자극제 처리 6개월 후 잎의 정유함량을 측정한 결과, 스트레스 자극제 처리에 의해 대체로 정류함량은 높아지는 경향을 보이고 있으나 자극제의 종류에 따라 변화가 없거나 또는 감소하는 경우도 있었다. 정유 증수효과에 영향을 미치는 수종별 최적 스트레스 자극제로 소나무는 치마버섯(SCC), 잣나무는 과산화수소수, 편백은 치마버섯(SCC), 화백은 ${\beta}-pyridone$이 선발되었으며, 특히 과산화수소와 ${\beta}-pyridone$은 공시한 4수종에 광범위하게 효과를 나타내는 스트레스 자극제였다. 그러나 뽕나무버섯(ARM)과 소나무잔나비버섯(FOP)의 잣나무 처리군 및 치마버섯(SCC)의 화백 처리군에서는 오히려 정유함량을 감소시켰다.

• KSBB Journal
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• 제13권5호
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• pp.569-576
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• 1998

The effects of carbohydrates, hormones and elicitors on both cell growth and betaine production were investigated in the cell cultures of Lycium chinense Mill. The maximum effect of glucose and sucrose was observed in cells cultured in the presence of 3% and 7% for cell growth and betaine production, respectively. The effect of hormones on cell growth and betaine production was prominent in the presence of 10 ${\mu}$M 2, 4-D, 10 ${\mu}$M NAA and 2.5 ${\mu}$M IAA, whereas cell growth and betaine production were excellent at 2.5 ${\mu}$M BA and 10 ${\mu}$M BA, respectively. Abiotic elicitors such as KCI, MnCl2 and NaCl exhibited an inhibitory role on cell growth in all treatment groups. Betaine production was increased according to increase of concentration of abiotic elicitors. methanol-soluble and insoluble components as biotic elicitor remarkably inhibited cell growth from 2 mg and 6 mg, respectively. Betaine production was increased maximally at 2 mg of biotic elicitors. When growth medium was switched to production medium at two stage culture, it resulted that cell fresh weight and dry weight decreased but betaine content increased about 2.2-fold.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.321-323
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• 2003

Hyoscyamine and scopolamine are the pharmaceutically valuable anticholinergic drugs. The aim of our study is to increase the contents of both hyoscyamine and scopolamine by means of elicitation. Various biotic elicitors derived from 3 fungi and 1 yeast were inoculated to the hairy root cultures. When homogenate and supernatant of elicitors treated with hairy root cultures, that of yeast elicitor was only increased scopolamine production. However, all of the fungal elicitors did not influenced the productions of scopolamine and hyoscyamine. Our results will contribute to mass production of tropane alkaloids by hairy root cultures of Scopolia parviflora.

• KSBB Journal
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• 제10권2호
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• pp.143-148
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• 1995

Taxus brevifolia 현탁세포배양에 서 항암제 taxol의 생산을 향상시키기 위해 5종류의 biotic elicitor 와 5종류의 abiotic elicitor, 2종류의 대사억제 및 8종류의 생장조절제를 세포배양 중에 첨가하여 효과 가 았는 물질을 선별하였다. T. brevifo/ia 현탁세포 배양의 대수증식기 말기인 10일째에 각각의 물질을 첨가하여 배양액 내의 taxol의 함량을 측정한 결과 steroid계 억제제 인 chlorocholine chloride를 처리 하였을 때 taxol의 생성이 현저히 증가되었다. Chloro choline chloride의 처리시기 및 처리농도를 다르게 하여 최적조건을 찾은 결과 9일째에 1mM을 첨가했 을 경우 taxol 생성이 가장 좋았다.

• Mycobiology
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• 제42권1호
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• pp.41-45
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• 2014

The effectiveness of three kinds of enzymes (chitinase, ${\beta}$-glucuronidase, and lysing enzyme complex), employed as elicitors to enhance the ${\beta}$-glucan content in the sawdust-based cultivation of cauliflower mushroom (Sparassis latifolia), was examined. The elicitors were applied to the cauliflower mushroom after primordium formation, by spraying the enzyme solutions at three different levels on the sawdust-based medium. Mycelial growth was fully accomplished by the treatments, but the metabolic process during the growth of fruiting bodies was affected. The application of a lysing enzyme resulted in an increase in the ${\beta}$-glucan concentration by up to 31% compared to that of the control. However, the treatment resulted in a decrease in mushroom yield, which necessitated the need to evaluate its economic efficiency. Although we still need to develop a more efficient way for using elicitors to enhance functional metabolites in mushroom cultivation, the results indicate that the elicitation technique can be applied in the cultivation of medicinal/edible mushrooms.

• 약학회지
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• 제44권1호
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• pp.60-65
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• 2000

The yeast extract, coconut water, safflower seed oil, arachidonic acid, linolenic acid, jasmonic acid and methyl jasmonate were added to Gamborg's B$_{5}$ medium. The changes on productivity of baccatin III were estimated every 30 minutes and the results were compared using the selected high yielding cell culture system of Taxus cuspidata. In most cases, the peaks of baccatin III productivity occured at 90~120 min after addition of elicitors. Among the compound elicitors, safflower seed oil showed the highest productivity of baccatin III. Also arachidonic acid and linolenic acid increased the baccatin III production.

• Journal of Microbiology and Biotechnology
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• 제1권3호
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• pp.220-226
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• 1991

Large and rapid increases in benzophenanthridine alkaloid production occured in suspension cultures of Eschscholtzia californica cells treated with elicitors. Response to different biotic elicitors showed that elicitors prepared from yeast extract, Collectotrichum lindemuthianum and Verticillium dahliae induced alkaloid formation. Highest alkaloid accumulation was obtained with $60\;\mu\textrm{g}$ of yeast extract elicitor per gram of fresh cell weight. In time course performance after elicitor addition, more than 40 hours were required to obtain saturated alkaloid accumulation. Compounded silicone fluid, an ideal accumulation phase for two-phase culture of E. californica, accumulated a large amount of alkaloids produced in a specific manner. Elicitation in two-phase culture clearly increased net alkaloid production as well as their concentrations in the accumulation phase.