Effect of Polysaccharide Elicitors on the Production of Decursinol Angelate in Agelica gigas Nakai Root Cultures

  • Cho, Ji-Suk (Department of Biological Engineering and Center of Advanced Bioseparation Technology, Inha University) ;
  • Kim, Ji-Yeon (Department of Biological Engineering and Center of Advanced Bioseparation Technology, Inha University) ;
  • Kim, Ik-Hwan (Graduate School of Biotechnology, Korea University) ;
  • Kim, Dong-Il (Department of Biological Engineering and Center of Advanced Bioseparation Technology, Inha University)
  • Published : 2003.04.01

Abstract

Root cultures of Angelica gigas Nakai were found to be sensitive to elicitation by poly-saccharide elicitors, such as methyl-$\beta$-cyclodextrin, glucan, carboxymethyl-$\beta$-chitin, chitosan, yeast extract and pectin. For the production of decursinol angelate, ca rboxymethyl-$\beta$-chitin and glucan were found to be the most efficient elicitors. The e nhanced accumulation of decursinol angelate was proportional to the increase of the phenylalanine ammonialyase (PAL) activity after the treatment with most of the elicitors. However, carboxymethyl-$\beta$-chitin treatment did not stimulate the PAL activity, despite the 1.6-fold increase in the decursinol angelate production.

Keywords

References

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