• 환경생물
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• 제29권4호
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• pp.265-273
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• 2011

Bacterial diversity based on the denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA gene sequences was determined for soil samples from two abandoned mine sites and the corresponding enrichment cultures using soil sample as key inoculum. Sequencing analysis of DGGE bands obtained from both the soil samples matched mostly with sequences of uncultured and newly described organisms, or organisms recently associated with the acid mine drainage environment. However, the enrichment of soil samples in ferrous sulfate and elemental sulfur media yielded sequences that were consistent with well-known iron- and sulfur-oxidizing acidophilic bacteria. Analysis of enrichment cultures of soil samples from Dalsung mine revealed abundant ${\gamma}$-$Proteobacteria$, whereas that of Gubong mine sample displayed acidophilic groups of ${\gamma}$-$Proteobacteria$, ${\alpha}$-$Proteobacteria$, $Actinobacteria$ and $Firmicutes$. Chemical elemental analysis of the mine samples indicated that the Dalsung site contained more iron and sulfate along with other toxic components as compared with those of the Gubong site. Biogeochemistry was believed to be the primary control on the acidophilic bacterial group in the enrichment samples.

• 분석과학
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• 제18권4호
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• pp.263-270
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• 2005

핵분열트랙은 우라늄, 플루토늄, 토륨 등의 방사성핵종 분석이나, 리튬이나 붕소와 같이 열중성자와 핵반응을 하는 원소분석에 매우 유용하다. 이 방법은 대기 중의 방사성핵종 분석을 통해 핵무기 실험을 검출한다거나, 우주공간의 중이온에 대한 선량측정이나 운석의 우주선 조사과정 추정, 원자력분야의 핵분열률 측정, 암석의 연대 및 역사를 파악하거나, tracer로 사용하는 등 여러 분야에 적용 가능하다. 현재 원자력연구소에서는 알파트랙기입법을 이용한 미량의 보론 분석이나, 핵분열트랙을 이용한 우라늄 등의 핵물질을 포함한 입자분석에 적용하고 있다. 본 총설에서는 트랙형성의 이론적 배경, 에칭된 트랙을 얻는 실제 과정 및 앞으로의 전망 등에 대해 살펴보았다.

• Macromolecular Research
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• 제12권6호
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• pp.586-592
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• 2004

Poly(glycidyl methacrylate)-grafted polyethylene microbeads (POPM) presenting epoxy groups were prepared by radiation-induced graft polymerization of glycidyl methacrylate on the polyethylene microbead. The obtained POPM was characterized by IR spectroscopic, X-ray photoelectrons spectroscopy (XPS), scanning electron microscope (SEM), and thermal analyses. Furthermore, the abundance of epoxy groups on the POPM was determined by titration and elemental analysis after amination. The epoxy group content was calculated to be in the range 0.29-0.34 mmol/g when using the titration method, but in the range 0.53-0.59 mmol./g when using elemental analysis (EA) after amination. The lipase was immobilized to the epoxy groups of the POPM under various experi­mental conditions, including changes to the pH and the epoxy group content. The activity of the lipase-immobilized POPM was in the range from 160 to 500 unit/mg-min. The activity of the lipase-immobilized POPM increased upon increasing the epoxy group content. The lipase-immobilized POPM was characterized additionally by SEM, elec­tron spectroscopy for chemical analysis (ESCA), and EA.

• Structural Engineering and Mechanics
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• 제62권3호
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• pp.345-355
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• 2017

The aim of the present study is to develop an elemental approach based on the differential quadrature method for free vibration analysis of cracked thin plate structures. For this purpose, the equations of motion are established using the classical plate theory. The well-known Generalized Differential Quadrature Method (GDQM) is utilized to discretize the governing equations on each computational subdomain or element. In this method, the differential terms of a quantity field at a specific computational point should be expressed in a series form of the related quantity at all other sampling points along the domain. However, the existence of any geometric discontinuity, such as a crack, in a computational domain causes some problems in the calculation of differential terms. In order to resolve this problem, the multi-block or elemental strategy is implemented to divide such geometry into several subdomains. By constructing the appropriate continuity conditions at each interface between adjacent elements and a crack tip, the whole discretized governing equations of the structure can be established. Therefore, the free vibration analysis of a cracked thin plate will be provided via the achieved eigenvalue problem. The obtained results show a good agreement in comparison with those found by finite element method.

• 한국환경과학회지
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• 제24권11호
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• pp.1541-1546
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• 2015

The objective of this research was to evaluate the toxicity of the industial xenobiotic Aroclor 1248 (A) and natural origin substances~elemental sulfur (S80) and oleic acid (OA) and their binary mixtures to V. fischeri bioluminescence during the prolonged exposure time (up to 60 min). The bioluminescence quenching test was used to determine the toxic effects. Full factorial experiment design and multiple regression analysis and the comparison of binary mixture effect with the sum of effects of individual chemicals were used for the evaluation of combined effects of toxicants. The analysis of general trend of mixture toxicity to bioluminescence showed that mixture toxic effects were reversible up to 60 min. Data analysis revealed different joint effects, which were depended on mixture composition. S80 enhanced toxic effect of A and acted additively with synergistic interaction. Hydrophobic OA in mixture with A acted antagonistically and in mixture with sulfur caused an additive effect with antagonistic component of interaction. It was concluded that low concentrations of natural toxic substances present in environmental samples as mixtures of chemicals can define the toxicodynamic character of industrial xenobiotics.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.263-263
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• 2017

Rice (Oryza sativa L.) is the world's third largest food crop after wheat and corn. Geographic authentication of rice has recently emerged as an important issue for enhancing human health via food safety and quality assurance. Here, we aimed to discriminate rice from six Asian countries through geographic authentication using combinations of elemental/isotopic composition analysis and chemometric techniques. Principal components analysis could distinguish samples cultivated from most countries, except for those cultivated in the Philippines and Japan. Furthermore, orthogonal projection to latent structure-discriminant analysis provided clear discrimination between rice cultivated in Korea and other countries. The major common variables responsible for differentiation in these models were ${\delta}^{34}S$, Mn, and Mg. Our findings contribute to understanding the variations in elemental and isotopic compositions in rice depending on geographic origins, and offer valuable insight into the control of fraudulent labeling regarding the geographic origins of rice traded among Asian countries.

• Mass Spectrometry Letters
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• 제9권4호
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• pp.105-109
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• 2018

A certified reference material (CRM) for the analysis of inorganic nutrients in nutritional supplements has been developed. Accurate mass fractions of chromium (Cr), iron (Fe), copper (Cu), and zinc (Zn) were determined by isotope dilution inductively coupled plasma mass spectrometry (ID ICP/MS). The measurement results were used to assign certified values for the CRM, which were metrologically traceable to the definitions of the measurement units in the International System of Units (SI). Production of a candidate reference material (RM) and the certification processes are summarized. Each nutrient in the CRM showed good homogeneity, which was estimated using relative standard deviations of the measurement results of twelve bottles in a batch. This CRM is expected to be an important reference to improve reliability and comparability of nutrient analyses in nutritional supplements and related samples in analytical laboratories.

• 한국표면공학회지
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• 제57권2호
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• pp.86-91
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• 2024

In this study, we investigated the effect of Na₂SiO₃ concentration on the Plasma Electrolytic Oxidation(PEO) film formation of Al6061 alloy. The morphology of the PEO films were examined by Optical Microscope(OM) and Scanning Electron Microscope(SEM). PEO film thickness increases as the Na₂SiO₃ concentration increases. The elemental analysis of PEO films was conducted using Dispersive X-ray Spectrometer(EDS). The cross-sectional elemental analysis result shows that the Si concentration tends to increase as the concentration of Na₂SiO₃ increases. X-Ray Diffraction(XRD) analysis was performed to confirm the degree of phase change according to Na₂SiO₃ concentration. In addition, Vickers hardness was measured to confirm the mechanical strength of the PEO film. As the concentration of Na₂SiO₃ increases, the hardness value also tends to increase.

• 한국환경과학회지
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• 제23권3호
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• pp.447-457
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• 2014

The number concentrations, the mass concentrations and the elemental concentrations of $PM_{10}$ have measured at Gosan site in Jeju, Korea, from March 2010 to December 2010. And the correlation and the factor analysis for the number, the mass and the elemental concentrations of $PM_{10}$ are performed to identify their relationships and sources. The average $PM_{10}$ number concentration is observed $246\;particles/cm^3$($35.7{\sim}1,017\;particles/cm^3$) and the average $PM_{10}$ mass concentration is shown $50.1{\mu}g/m^3$($16.7{\sim}441.4{\mu}g/m^3$) during this experimental period. The number concentrations are significantly decreased with increasing particle size, hence the concentrations for the smaller particles less than $2.5{\mu}m$($PM_{2.5}$) are contributed 99.6% to the total $PM_{10}$ number concentrations. The highest concentration of the 20 elements in $PM_{10}$ determined in this study is shown by S with a mean value of $1,497ng/m^3$ and the lowest concentration of them is found by Cd with a mean value of $0.57ng/m^3$. The elements in $PM_{10}$ are evidently classified into two group based on their concentrations: In group 1, including S>Na>Al>Fe>Ca>Mg>K, the elemental mean concentrations are higher than several hundred $ng/m^3$, on the other hand, the concentrations are lower than several ten $ng/m^3$ in group 2, including Zn>Mn>Ni>Ti>Cr>Co>Cu>Mo>Sr>Ba>V>Cd. The size-separated number concentrations are shown positively correlated with the mass concentrations in overall size ranges, although their correlation coefficients, which are monotonously increased or decreased with size range, are not high. The concentrations of the elements in group 1 are shown highly correlated with the mass concentrations, but the concentrations in group 2 are shown hardly correlated with the mass concentrations. The elements originated from natural sources have been predominantly related to the mass concentrations while the elements from anthropogenic sources have mainly affected on the number concentrations of $PM_{10}$.

• 대한기계학회논문집
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• 제14권1호
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• pp.1-12
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• 1990

본 연구에서는 자유단조용 프레서를 이용하여 돌출부가 있는 비대칭 중공형단 조 제품을 생산할 수 있도록 금형장치를 설계.제작하고, Fig.1에서 보는 바와 같이 경 사진 돌출부가 있는 비축대칭 복합단조에 UBET를 적용하여 해석하였다. 단조시편은 원주형태를 가지도록 하였다. 즉 원주형 소재가 요구되는 비축대칭 단조제품으로 변 형되는 과정에서 단조하중, 재료가 돌출부를 충만하도록 하는 유동특성, 재료가 돌출 부로 차들어가는 속도 등에 영향을 주는 인자(예:펀치 직경의 크기, 금형 상하면의 각 도)의 특성을 이론적으로 해석하고 실험으로 확인하였다.