• Parasites, Hosts and Diseases
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• v.34 no.3
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• pp.211-213
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• 1996

Malaysian, Ajricn and Thai PZQsmodiumJnkipamm isolates were cultured in uiko by the Tracer and Jensen method (1976, 1977) and were later cloned by the limiting dilution method (Rosario, 1981), Forty-eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.

• The Korean Journal of Ecology
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• v.21 no.3
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• pp.301-307
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• 1998

Intraspecific and interspecific isozyme variations and their relationship of 16 local populations in 3 species of Phyllostachys, that is, P. bambusoides, P. nigra var. henonis and P. pubescens were investigated by multi-variate analysis. Leaf isozymes of Phyllostachys such as 6-PGD (6-phosphogluconate dehydrogenase), MDH (malate dehydrogenase), PGI (phosphoglucoisomerase), PRX (peroxidase), PGM (phosphoglutamase), IDH (isocitrate dehydrogenase) showed electrophoretic variations in the number of zymotypes (7, 6, 6, 9, 3 and 5, respectively). In the cluster analysis on the isozymic characteristics, 16 populations were classified into 3 species at the euclid genetic distance of 2.041. P. nigra var. henonis and P. bambusoides were clustered first at 2.813 and then P. pubescens at 3.001. So far, 3 local types of intraspecific ariation were found in P. nigra var. henonis and P. bambusoides.

• Journal of Magnetics
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• v.16 no.4
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• pp.444-448
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• 2011

In the present study, we separated the marker particles from the suspending particle mixture solution using isotacho-electrophoresis technique, a novel quantitative ionic particle separation method, in the microchannel. A multiple stacking zone of the suspending particle was visualized with variations in electric field strength, pH value and concentration of the ionic solution. In particular, the electrophoretic mobility of ionic particle (fluorescein) was estimated based on the electrophoretic velocity value measured by the particle image velocimetry. As a result, isotacho-electrophoresis zones were clearly visualized as going downstream in the electric field. The particle migration velocity increased proportional to the applied voltage increase; it was also affected by the pH value variations in the ionic solution.

• Korean journal of applied entomology
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• v.23 no.2 s.59
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• pp.102-108
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• 1984

These researches were carried out to investigate the morphology of different species of Trichoderma and the possibilities of differentiation of the species of Trichoderma by electrophoretic methods. Variations between the isolates of a species of Trichoderma indicate the genetical differences, also isozyme and protein patterns will be useful to investigate genetical variations betweens the isolates. It might be possible that distinct bands of isozymes of esterase, phosphotase, catalase, catalase differentiate species of Trichoderma.

• The Korean Journal of Malacology
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• v.16 no.1_2
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• pp.11-16
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• 2000

The electrophoretic banding patterns of 6-phosphogluconate dehydrogenase (PGD) in the two different chromosomal ploidy groups of Gyraulus were compared. The monomeric or dimeric banding patterns or allelic variations in a locus of PGD were observed in four diploid populations (Osan, Sohre, Kimpo and Kangwha) of G. convexiusculus occurring in Korea, whereas the isozyme banding patterns encoded by at least 3 different loci were shown in the tetraploid populations of G. (Torquis) groups collected from Michigan, the U.S.A. Of 3 different tetraploid groups, G. (T.) circumstriatus group showed 3 monomorphic isozyme banding patterns, and the other 2 populations showed some allelic variations. Such results provided good evidence to differentiate tetraploid subgenus Torquis group from the diploid Gyraulus populations.

• The Korean Journal of Malacology
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• v.2 no.1
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• pp.30-34
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• 1986

Genetic variations of Semisulcospjra gottschei in Korea were investigated by means of starch gel-electrophoresis. The results are as follows; 1) Eight loci of ${\alpha}$-Gpd, Mdh-2, Pept-1, Pgm-2, Gp-1, Gp-2, Gp-3 and Gp-4 were monomorphic, and 8 loci of Mpi, Mdh-1, Pgi, Got, Pept-2, Pept-3, Pgm-1 and Sdh showed genetic variations. 2) Means of allele per locus ($\bar{A}$) and polymorphism (P) were revealed 0.64 and 0.44, respectively. The value of heterozygosity ($H_G$) showed a little more than heterozygosity $H_D$. ($H_D$=0.09, $H_G$=0.13) 3) Genetic variation appears to be due to loci of Got, Pept, Pgi, and Sdh, all of which of the $H_D$ values are high.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.55-59
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• 1982

With naked barley varieties which are different in their spring habits, variations in seed proteins during ripening were checked by acrylamide gel electrophoresis. In case of 7% acrylamide gel among protein bands ⅰ-band was observed only in both Sedohadaka (intermediate type) and Wanju (spring type) throughout the ripening period, and j-band was detected in all the three varieties until 33 days after heading, but not in Nonsangwa (winter type) thereafter. In case of 6M urea-7% acrylamide gel z-band was traced only in Nonsangwa, contrary to i-band, throughout the ripening period. U-band was observed in all the three varieties until 23 days after heading, but not in Nonsangwa thereafter. X-band showed opposite from u-band. Throughout the ripening period Sedohadaka was significantly more similar in the electrophoretic patterns to Wanju than to Nonsangwa.

• Korean Journal of Ichthyology
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• v.4 no.1
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• pp.96-104
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• 1992

The electrophoretic analysis, variations of protein and nucleic acid contents with sexual maturation, of the liver tissue of male, and of the muscle tissue of male and female B. pectinirostris(Linnaeus) were investigated. As the result of protein analysis of the liver and muscle tissue of male by the SDS-PAGE electrophoretic analysis, the protein of the liver tissue of male was presented 15.8-101.5kd in molecular weight, and appeared 19 bands in all. The protein bands of the muscle tissues of male and female were almost same, their bands were presented 16.9-116.5kd in molecular weight, and appeared 23 bands in all. And in the liver tissue, the protein contents of the liver tissue of male appeared the maximum in June(the mature stage) and afterwards decreased gradually in July(the spawning stage) and August(the degenerative stage). In the muscle tissues of male and female protein contents were shown the maximum in June, and afterwards decreased gradually, the minimum in July, increased a little in August. The total RNA contents of the liver tissue of male, and their contents of the muscle tissue of male and female appeared the maximum in June(the mature stage), and afterwards decreased gradually in July(the spawning stage) and August(the degenerative stage).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.4
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• pp.334-341
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• 1990

The electrophoretic variations in protein and some enzymes during stratification of Panax ginsseng seeds were checked in relation to the differentiation and development of immature embryo, Seed-protein on PAGE showed eight major bands during the period of investigation, Four bands of Rf : 0.12, 0.24, 0.66 and 0,72 remained in dark stained condition, whereas the remainders (Rf : 0.40, 0.48, 0.56 and 0,76) became lighter in the staining condition as the treatment period was prolonged, In embryo, which was separately sampled from 75 days after the treatement, the only band of Rf 0.76 was not observed, In peroxidase, the number of bands was gradually increased and came to four bands at 45 days after treatment, and their staining color became gradually darker until the time of final samling. Also, the embryo had the same patterns as those in the intact seed. Esterase showed four bands during the period of investigation. As the treatment period was prolonged, the degree of staining condition came to darker in the band of Rf 0.36, but lighter in the band of Rf 0.56. The band of Rf 0.20 was not detected in the intact seed at 90 days after the treatment, but clearly observed in embryo. Glutamate dehydrogenase had only two bands: one (Rf : 0.16) was detected only at 60 days after the treatment and the other (Rf: 0.14) was always observed throughout the period, and showing simple pattern in the electrophoretic variation. Glutamate-oxaloacetate transaminase showed the only band of Rf 0.36 during the period of investigation and even in embryo, and showing the simplest pattern, indeed.

• Journal of Aquaculture
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• v.16 no.2
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• pp.69-75
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• 2003

Genetic variability and population structure of 11 natural ayu, Plecoglossus altivelis populations and one hatchery stock were assessed by starch gel electrophoretic analysis with 10 enzyme coding loci. Three loci were polymorphic (lower than 0.95 in major allele frequency) in natural populations,2 loci in hatchery stock. The average number of alleles per locus was 1.38. Observed heterozygosities ranged from 0.0235 to 0.088 (0.055 on the average) in natural population while 0.0925 in hatchery stock. The genetic distance among natural populations measured 0.000047-0.005407 and no significant differentiation was observed among them. On the other hand, a signifcant genetic distance was found between natural populations and the hatchery stock with measuring 0.002032-0.O08605. The results in this study suggest that the hatchery stock has diverged from natural populations, and also that careful to maintain sustainable and effective population size (parents number) should be made.