• Title/Summary/Keyword: electrophoretic comparison

Search Result 41, Processing Time 0.029 seconds

Comparison of electrophoretic karyotypes in fusarium

  • Min, Byung-Re
    • Journal of Microbiology
    • /
    • v.33 no.4
    • /
    • pp.334-338
    • /
    • 1995
  • The electrophoretic karyotypes of 6 species in different Fusarium sections were examined by using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Intact chromosomal DNA was prepared from protoplasts and up to 9 distinct bands were separated on 0.7% or 0.8% agarose gel under several different conditions. Putative chromosome numbers varied from 6 to 9 amd polymorphic karyotypes were observed in different Fusarium sections. Using Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as standards, the sizes of the Fusarium spp. chromosomes were estimated. The electrophoretic karyotypes of F. moniliforme and F. subglutinans (section Liseola) were similar. Unidentified filamentour fungi, F. beomiforme was much closer to F. axysporum (section Elecgans) in karyotype and the karyotypes of F. napiforme were more similar to those of section Liseola than any other sections. F. graminearum (section Discolor) had a distinctive electrophoretic karyotype.

  • PDF

Electrophoretic Karyotyping by PFGE in the Genus Fusarium (Fusarium속에서 PFGE를 이용한 Electrophoretic Karyotyping)

  • Min, Byung-Re;Jung, Jin-Sook;Choi, Yong-Keel
    • The Korean Journal of Mycology
    • /
    • v.26 no.2 s.85
    • /
    • pp.135-143
    • /
    • 1998
  • Contour-clamped homogeneous electric field gel electrophoresis was used to establish electrophoretic karyotype for 10 species of Fusarium sections Sporotrichiella, Liseola, Gibbosum, Discolor and Martiella. Intact chromosomal DNA was isolated from fungal protoplast and separated under various conditions according to their size in order to improve DNA separation. The numbers of chromosome-sized DNA molecules for individual species ranged from 5-13, with individual chromosomes ranging from 0.78 Mb to 7.20 Mb in size. The total genome DNA size of each species was estimated at about 18.32 Mb to 48.20 Mb. Comparison of karyotype profiles following Southern hybridization analysis with a randomly selected genomic probe of F. oxysporum formae speciales litii was carried out.

  • PDF

Separation of Barley Seed Proteins by Disc Electrophoresis (Disc electrophoresis에 의한 대맥종자단백질의 분리)

  • 최종열
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.10
    • /
    • pp.91-97
    • /
    • 1971
  • Fifteen samples of barley seed proteins representing winter 6-rowed, spring 6-rowed and spring 2-rowed group were separated by disc electrophoresis. Photographic, densitometric and diagrammatic representation were presented to make comparison possible. Eighteen to twenty one bands were detected in each gel. Electrophoretic spectrum of each variety was highly variety specific showing that the spectrum is the reflection of hereditary constituents of a organism. Related variety showed identical or nearly identical pectrum. All winter 6-rowed barley showed very faint B2 band, while spring 6-rowed and spring 2-rowed barley showed dense or less dense B2 band. All spring barley showed B3 band, while all winter barley showed B4 band.

  • PDF

Comparison of Electrophoretic Isozyme Band Pattern of Pleurotus spp. in Korea -I. Homogeneous Gel- (한국산 느타리버섯(Pleurotus spp.)의 전기영동 Isozyme Band Pattern 비교 -I. Homogeneous Gel-)

  • Park, Yong-Hwan;Byun, Myung-Ok;Hiroshi, Fujii
    • The Korean Journal of Mycology
    • /
    • v.16 no.2
    • /
    • pp.87-94
    • /
    • 1988
  • Electrophoretic isozyme patterns from mycelia, primordia, cap and stem of Pleurotus spp. collected in Korea were compared. Primordia, cap and stem of fruitbody showed very similar isozyme patterns but mycelial isozyme patterns were different from those of fruitbody. Isozyme patterns of malate dehydrogenase, acid phosphatase in Pleurotus ostreatus collected from different regions in Korea were similar but those of esterase, peroxidase, leucine amino peptidase and superoxide dismutase were different. Interspecific comparison of esterase isozyme patterns among Pleurotus ostreatus P. cornucopiae and, P. florida was very different and may be valuable subsidiary tool to conventional taxonomic techniques for identifying species of Pleurotus. A dendrogram by similarities of isozyme band pattern was presented.

  • PDF

An Analysis of Reflectivity and Response Time by Charge-to-Mass of Charged Particles in an Electrophoretic Display

  • Kim, Young-Cho
    • Transactions on Electrical and Electronic Materials
    • /
    • v.17 no.4
    • /
    • pp.212-216
    • /
    • 2016
  • A reflective electronic display that uses negatively and positively charged particles has excellent bistability, a welldefined threshold voltage, and an extremely fast response time in comparison with other reflective displays. This type of display shows images through the movement of charged particles whose motion depends on the value of q/m (charge per mass for a particle). However, the ratio q/m can easily be changed by the forces acting on the charged particles in a cell of the panel and by friction that occurs after mixing oppositely charged particles and in the particle-insertion process. In this study, we propose a method to determine the appropriate range of q/m by using the reflectivity and response time of charged particles to modify q/m. In this manner, the electrical and optical properties of reflective displays are improved.

A rapid method for sodium dodecyl sulfate polyacrylamide gel electrophoresis evaluation (Sodium dodecyl sulfate polyacrylamide gel 전기영동 결과의 경제적인 평가 방법)

  • Page, C.;Hamelin, C.
    • Korean Journal of Microbiology
    • /
    • v.27 no.4
    • /
    • pp.436-438
    • /
    • 1989
  • We propose a method for rapid evaluation and permanent record of sodium dodecyl sulfate polyacrylamide gel electrophoretic runs. This method is based on the photocopy process, rather than on photography and requires no extensive or expensive investment. Comparison of a print obtained through this method and a 35mm photography indicated, on a balanced gel, equal sensitivity.

  • PDF

Study on the Effect of the SDS on the Behavior of Anionic Surfactants in Electrophoresis (전기영동에서 SDS가 음이온 계면활성제의 거동에 미치는 영향에 대한 고찰)

  • Lee, Ji-Eun;Lee, Myeong-Sok;Jeong, Hyuk
    • Analytical Science and Technology
    • /
    • v.14 no.4
    • /
    • pp.356-363
    • /
    • 2001
  • A basic study of the effect of the SDS on the behavior of the anionic surfact in electrophoresis was performed. Having been interacted with SDS micelle, long chain carboxylates and sulfonates which have more than 10 carbons have been suffered larger changes in their apparent mobilities. Based on the result of the comparison among apparent, electroosmotic and electrophoretic mobilities, it is demonstrated that the major cause for the variation of the apparent mobilities of the anions is the variation of the electrophoretic mobilities.

  • PDF