• 제목/요약/키워드: electric fusion

검색결과 186건 처리시간 0.023초

초소형 세포융합기구의 제작을 위한 기초연구 (원형질체 준비 및 전계인가방식에 관한 연구를 중심으로) (A Basic Study for the Fabrication of Micro Cell-Fusion-Device)

  • 이상훈;차현철;김용욱;김용권
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1993년도 하계학술대회 논문집 A
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    • pp.287-289
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    • 1993
  • Recently semiconductor technology is widely used in biotechnology fields and one of the represetatives is electric cell-fusion-device. In this paper. some basic researches required in the development of this fusion system was performed and they are as follows: 1) preparation of protoplast and selection of the fittest plant cell, 2) development of high frequency AC power supply for cell-alignment by using class-E resonant circuit. As a result, cabbage cell is selected and pearl-chain, which shows cell-alignment. can be observed by the applied AC field.

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Neuro-fuzzy modeling of deformation parameters for fusion-barriers

  • Akkoyun, Serkan;Torun, Yunis
    • Nuclear Engineering and Technology
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    • 제53권5호
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    • pp.1612-1618
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    • 2021
  • The fusion-barrier distribution is very sensitive to the structure of the colliding nuclei such as nuclear quadrupole and hexadecapole deformation parameters and their signs. If the nuclei that enter the fusion reaction are deformed, the barrier problem becomes complicated. Therefore the deformation parameters are taken into account in the calculations. In this study, Neuro-Fuzzy approach, ANFIS, method has been used for the estimation of ground-state quadrupole (𝜀2) and hexadecapole (𝜀4) deformation parameters for the nuclei. According to the results, the method is suitable for this task and one can confidently use it to obtain the data that is not available in the literature.

Fusion technology of artifacts considering environmental recycling for sustainability

  • Fujita Toyohisa
    • 한국지구물리탐사학회:학술대회논문집
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    • 한국지구물리탐사학회 2003년도 Proceedings of the international symposium on the fusion technology
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    • pp.563-568
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    • 2003
  • Recently, the recycled amount of electric, mechanical parts, and appliances in artifacts has increased. These products use valuable rare metals such as platinum group metals and gold, which are included occasionally as additives. Rare metals are maldistributed in the world and most of them are produced in small quantities. A small amount of rare metals used in the appliances causes a large loss of rare metal resources because of the lack of an economically recycling method. The present recycling technologies including physical and chemical separation methods that are considered for recycling of electric, mechanical parts and appliances.

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핵융합 촉진 방법을 이용한 새로운 핵융합 장치의 개발 (Development of the New nuclear fusion devices Using Method of promoting nuclear fusion)

  • 김기성
    • 한국조명전기설비학회:학술대회논문집
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    • 한국조명전기설비학회 2005년도 학술대회 논문집
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    • pp.151-155
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    • 2005
  • 지금까지의 핵융합 장치는 자기우물과 열역학을 바탕으로 한 토카막 장치가 수소핵융합을 하였으나 고온의 플라스마를 장시간 동안 가둠이란 현실적으로 불가능하여 성공사례가 없었다. 본 핵융합 촉진 방법을 이용한 새로운 핵융합 장치는 토카막 장치의 토로이달 자기장용 알루미늄코일 내에 토러스 철심 C형 Core Block과 토로이달 알루미늄코일을 넣어 토로이달 자기장을 모으고 토로이달 전류 흐르도록 하여 토러스 Core의 일부가 절개된 간극사이에 핵융합로를 배치하고 융합원료(전해액)를 토로이달 전류로 전기분해 한다. 전원과 토로이달 자계용 코일, 토로이달 코일 융합원료로 직렬회로로 이루어져 있어 토로이달 전류는 필라멘트 전류로 되어 융합원료에 투입된다. 필라멘트 전류사이에는 자기흡입력이 외부의 입력전력으로 계속 증대되어 쿨롱에 힘을 넘어 핵융합에 이르고 그로인해 질량결손이 생겨 아인슈타인의 질량에너지$(E=mC^2)$가 빛에너지와 열에너지로 방출됨을 확인했다.

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핵이식 기법을 이용한 한우 쌍태생산에 관한 연구 I. Ovum pick-up(OPU), 전기적 세포융합 및 체외배양 기법을 이용한 복제수정란 생산 (Induction of twinning in Korean native cattle by transfer of nuclear transplanted embryos I. Embryo cloning using ovum pick-up(OPU), electric cell fusion and in vitro culture system)

  • 황우석;신태영;노상호;이병천
    • 대한수의학회지
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    • 제38권1호
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    • pp.209-216
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    • 1998
  • The purpose of present study is to improve the efficiency of fusion and the developmental rates of nuclear transplanted embryos to produce genetically identical twins from Korean native cattle. The diameter of aspirated follicles had no significant effect on the recovery rates of oocytes collected by ovum pick-up technique. The fusion rates of nuclear transplanted embryos were significantly higher in 50 and $100{\mu}s$ DC duration groups(73.3 and 72.0% ; respectively) than that in $30{\mu}s$ group(55.6% ; p<0.05). The cleavage rates of nuclear transplanted embryos appeared to be significantly higher in donor nuclei derived from in vivo (65.0%) than in those from in vitro (50.5% ; p<0.01), but the developmental rates to morulae and blastocysts were not significantly different between them(13.7 vs 10.9%, respectively).

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Synthesis and Structural Properties of $VO_2$ Thin Films

  • Jin, Zhenlan;Park, Changin;Hwang, Inhui;Han, S.W.
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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    • pp.190.2-190.2
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    • 2013
  • Vanadium dioxide ($VO_2$) has been widely attracted for academic research and industrial applications due to its metal-insulator transition (MIT) temperature close to room temperature. We synthesized VOx film on (0001) sapphire substrate with vanadium target (purity: 99.9%) using DC magnetron sputtering in Ar ambience at a pressure of $10^{-3}$ Torr at $400{\sim}700^{\circ}C$. The VOx film subsequently was annealed at difference temperatures in ambience of Ar and $O_2$ gas mixture at $60{\sim}800^{\circ}C$. The structural properties of the films were investigated using scanning electron microscopic (SEM), x-ray diffraction (XRD) and x-ray absorption fine structure (XAFS) measurements. SEM reveal that small grains formed on the substrates with a roughness surface. XRD shows oriented $VO_2$(020) crystals was deposited on the $Al_2O_3$(006) substrate. From I-V measurements, the electric resistance near its MIT temperature were dramatically changed by ${\sim}10^4$ during heating and cooling the films. We will also discuss the temperature-dependent local structural changes around vanadium atoms using XAFS measurements.

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전극표면에서 액적의 충전현상과 이를 이용한 액적의 이동 방법에 관한 연구 (Research on the Electrical Charging of a Water Droplet on the Electrode and Droplet Actuation Method using Electrical Charge)

  • 정용미;오현창;강인석
    • 한국전산유체공학회:학술대회논문집
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    • 한국전산유체공학회 2008년도 춘계학술대회논문집
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    • pp.666-669
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    • 2008
  • Droplet in miniaturized microfluidic systems have received much focused attention recently. In this work, electrical charging phenomenon of a conducting water droplet on the electrode under the dc electric field is studied and using this phenomenon droplet actuation method for microreactor applications is experimentally demonstrated. To find effects of key factors, the effects of electric field, medium viscosity, and droplet size are investigated. A scaling law of charging for the conducting droplet is derived from the experimental results. Unlike the case of a perfect conductor, the estimated amount of electrical charge ($Q_{est}$) of a water droplet is proportional to the 1.59 power of the droplet radius (R) and the 1.33 power of the electric field strength (E). (For a spherical perfect conductor, Q is proportional to R2 and E.) It is thought that the differences are mainly due to incomplete charging of a water droplet resulted from the combined effect of electrochemical reaction at electrode and the relatively low conductivity of water. Using this phenomenon, we demonstrate the transport of the charged droplet and fusion of two oppositely-charged droplets. When electric field is subjected sequentially on the electrode, the charged droplet is transported on the electrode. For the visualization of fusion of charged droplets, the precipitation reaction is used. When subjected to a DC voltage, two droplets charged are moving and merging toward each other due to the Coulombic force and chemical reaction is simultaneously occurred by coalescence of droplets. It may be due to the interchange effect of charge. It is shown that the droplet can be used for microreactor where transporting, merging etc. of reagents constitute unit operation.

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Comparison of Microtubule Distributions between Somatic Cell Nuclear Transfer and Parthenogenetic Porcine Embryos

  • Park, Joo-Hee;Kwon, Dae-JinK;Lee, Beom-Ki;Hwang, In-Sun;Park, Choon-Keun;Yang, Boo-Keun;Cheong, Hee-Tae
    • Reproductive and Developmental Biology
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    • 제33권1호
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    • pp.13-18
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    • 2009
  • The aim of this study was to examine the microtubule distributions of somatic cell nuclear transfer (SCNT) and parthenogenetic porcine embryos. Porcine SCNT embryos were produced by fusion of serum-starved fetal fibroblast cells with enucleated oocytes. Reconstituted and mature oocytes were activated by electric pulses combined with 6-dimethlyaminopurine treatment. SCNT and parthenogenetic embryos were cultured in vitro for 6 days. Microtubule assembly of embryos was examined by confocal microscopy 1 hr and 20 hr after fusion or activation, respectively. The proportions of embryos developed to the blastocyst stage were 25.7% and 30.4% in SCNT and parthenogenetic embryos, respectively. The frequency of embryos showing $\beta$-tubulins was 81.8% in parthenogenetic embryos, whereas 31.3% in SCNT embryos 1 hr after activation or fusion. The frequency of the embryos underwent normal mitotic phase was low in SCNT embryos (40.6%) compared to that of parthenogenetic ones (59.7%) 20 hr after fusion or activation (p<0.05). The rate of SCNT embryos with an abnormal mitosis pattern is about twice compared to that of parthenogenetic ones. The spindle assembly and its distribution of SCNT embryos in the first mitotic phase were not different from those of parthenogenetic ones. The result shows that although microtubule distribution of porcine SCNT embryos shortly after fusion is different from parthenogenetic embryos, and the frequency of abnormal mitosis 20 hr after fusion or activation is slightly increased in SCNT embryos, microtubule distributions at the first mitotic phase are similar in both SCNT and parthenogenetic embryos.

Solid-phase Refolding of Poly-lysine Tagged Fusion Protein of hEGF and Angiogenin

  • Park Sang Joong;Ryu Kang;Suh Chang Woo;Chai Young Gyu;Kwon Oh Byung;Park Seung Kook;Lee Eun Kyu
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제7권1호
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    • pp.1-5
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    • 2002
  • A fusion protein, consisting of a human epidermal growth factor (hEGF) as the recognition domain and human angiogenin as the toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as inclusion body in recombinant E. coli, and when the conventional, solution-phase refolding process was used the refolding yield was very low due to severe aggregation. It was probably because of the opposite electric charge at a neutral pH resulting from the vastly different pI values of each domain. The solid-phase refolding process that exploited the ionic interactions between ionic exchanger surface and the fusion protein was tried, but the adsorption yield was also very low, below $ 30\%$, regardless of the resins and pH conditions used. Therefore, to provide a higher ionic affinity toward the solid matrix, six lysine residues were tagged to the N-terminus of the hEGF domain. When heparin-Sepharose was used as the matrix, the adsorption capacity increased 2.5-3 times to about $88\%$. Besides the intrinsic affinity of angiogenin to heparin, the poly-lysine tag provided additional ionic affinity. And the subsequent refolding yield increased nearly 13-fold, from ca. $4.8\%$ in the conventional refolding of the untagged fusion protein to $63.6\%$. The process was highly reproducible. The refolded protein in the column eluate retained RNase bioactivity of angiogenin.

ASM을 이용한 전기 이륜차 동력 특성 해석에 관한 연구 (A Study on Power Characteristic of Electric Motorcycle using ASM)

  • 이태형;김병우;김용은
    • 한국자동차공학회논문집
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    • 제21권3호
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    • pp.58-65
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    • 2013
  • In this paper, we propose that a model based design for an electric motor cycle system using ASM (Automotive Simulation Models). Before prototyping a realistic electric motorcycle, a reliable simulation program is required to test the capacities of the power sources and optimize the parameters of an electric motorcycle. Because ASM is based on Simulink, we can design the drivetrain and powertrain of the vehicle model systems easily. To verify the electric motorcycle system analysis of design parameters such as max power, capacity, state of charge and slope angle is carried out by the simulation and experimental method. The predicted results by the development model were in good agreement with the experimentally obtained results. Therefore, the proposed electric motorcycle model can effectively reduce the expenses during the designing of an electric motorcycle system.