• Asian-Australasian Journal of Animal Sciences
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• v.2 no.2
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• pp.103-114
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• 1989

Reciprocal crosses were conducted between three strains of Taiwan Country chickens, developed in the National Chung-Hsing University, and two strains of Single Comb White Leghorns, developed in the Taiwan Livestock Research Institute. Traits studied were growing performances, laying performances, egg quality traits and traits concerning disease resistance, including resistance to Marek's disease virus and immune responses to Newcastle disease virus vaccine and to sheep red blood cell. Results indicated that laying performances of Taiwan country chickens were much inferior to White Leghorns, but they matured earlier, their eggs had better shell strength and larger proportion of yolk, and their general disease resistance was much better than White Leghorns. Heterosis were found in laying performances and egg quality traits. The heterosis in laying traits was so large that the hybrid laid as many eggs and as large eggs as did pure strains of White Leghorns. Strategies on the improvement of native chickens and the utilization of genetic merits of native chickens were also discussed.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.3
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• pp.500-504
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• 2001

Gamma irradiation was applied to reduce egg allergy. Ovalbumin (OVA), an egg white protein, was used as model allergen and was gamma-irradiated at 3, 5, or 10 kGy in an aqueous state (2.0 mg/mL). The changes in allergenic and antigenic properties of OVA resulted from gamma irradiation were monitored by ELISA with serum from egg-hypersensitive patients (H-IgE), and mouse monoclonal IgG (M-IgG) or rabbit polyclonal IgG (R-IgG). The binding ability of H-IgE to irradiated OVA was dose-dependently reduced. However, IgGs from animal did better recognize 3 or 5 kGy-irradiated OVA. In the evaluation of immune reactivity using blind test, the reactivity of H-IgE rapidly decreased depending upon the irradiation dose. However, the reactivities of M-IgG and R-IgG was higher at 5 and 3 kGy-irradiated OVA than non-irradiated control. The results provide a new possibility to use irradiation process for reducing the allergenicity of egg white.

• Korean Journal of Food Science and Technology
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• v.41 no.2
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• pp.167-172
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• 2009

The principal objective of this study was to determine the optimum manufacturing conditions of egg white with high foaming property for the production of angel food cakes. The egg whites were desugarized and powdered at predetermined pHs and drying temperatures. The physicochemical properties of the produced cakes were measured and sensory evaluations were conducted on a 9-point scale. The volume and textural properties of cakes produced from spray-dried egg whites did not differ from those of the cakes prepared from the control egg whites. The pH of batter and the specific gravity of foam and batter with egg white powder at pH 6.74/66.5$^{\circ}C$ did not differ from those of the control, whereas the one prepared with commercial powder lost the characteristics of cake to a significant degree. The overall acceptance scores of egg white powder of pH 6.74/66.5$^{\circ}C$ and commercial powder were 6.25 and 2.33 as compared to the control (6.42). These results indicate that the egg white powder that was desugarized, pH-adjusted to 6.74, and dried at 66.5$^{\circ}C$ might be utilized to prepare angel food cakes with the desirable physicochemical and sensory attributes.

• Journal of Food Hygiene and Safety
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• v.4 no.1
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• pp.29-36
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• 1989

In order to investigate the Storage time of chicken eggs, several physico-chemical tests from chicken eggs store at 5, 13 and 27$^{\circ}C$ were examined. Egg samples were collected from six poultry farms. Egg stored at 5$^{\circ}C$ based on the depth of air cell and specific gravity, were all acceptable until 17 days ; on the egg yolk coefficient and pH of the egg white and egg yolk until about 10 days. Egg stored at 13$^{\circ}C$, based on the depth of air cell. were acceptable by about 10 days of storage, but on the other physico-chemical tests by about 7 days. Egg samples stored at room temperature(about 27$^{\circ}C$) base on the depth of air cell, were acceptable by about 5 days of storage ; on the specific gravity by 4 days ; and on the egg yolk coefficient and pH of the egg yolk and egg white by 3 days. The results of this study showed that egg stored at 5$^{\circ}C$ were considered acceptable by 10 days of storage ; at 13$^{\circ}C$ by 7 days ; at room temperature (27$^{\circ}C$) by 3 days.

• Korean Journal of Poultry Science
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• v.23 no.1
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• pp.19-26
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• 1996

The present study was conducted to estimate average weights, and corrlation and regression coefficients among various traits of eggs obtained from Korean native and imported chicken. Data were obtained from 360 layers (180 of Korean native origin, and 180 of imported Decalb Brown) between November 8, 1994 to February 8, 1995. The results obtained are summerized as follows : 1. The mean weights of whole egg, yolk, dense white, thin white, and egg shell of native chicken were lighter (P<0.01) than those of Decalb Brown. 2. The coefficients of variability in each egg trait were lower in native chicken than in Decalb Brown, indicating the higher ability of native chicken to adapt to the local environment. 3. The phenotypic correlation coeffcients between whole egg and weights of yolk, dense white, thin white, and eggshell were 0.842∼0.992(P<0.01) in native chicken, and 0.865∼0.985(P<0.01) in Decalb Brown. 4. The average eggshell thicknesses of native and imported Decalb Brown were 362.5 $\mu\textrm{m}$ and 407.9 $\mu\textrm{m}$, respectively. 5. The regression coefficients of most of the traits of eggs from native chicken were lower than those from Decalb Brown layers, suggesting that Korean native chicken should be improved further in various egg traits measured in this study.

• Korean Journal of Poultry Science
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• v.7 no.2
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• pp.47-53
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• 1980

This study was carried out to investigate the variations in the thickness of egg shell and egg shell membrane of Quail eggs from July 25, 1978 to September 7, 1978. No. of eggs used in this experiment was 520 okay and all the eggs were purchased from a farm located in Jinju city. The results obtained are summarized as follows: 1. Egg shells of quail were classified as white egg shell with large spots, egg shell with large spots, violet egg shell with small spots and beige egg shell with small spots by the exterior shell colour and their average egg weights were 8.993g, 8.866g, 8.403g and 8.109g, respectively. 2. Average egg shell thickness of white egg shell with large spots, egg. shell with large spots, violet egg shell with small spots and beige on shell with small spots were 0.171mm, 0.169mm, 0.160mm, and 0.156mm, respectively. 3. Average eggs shell membrane of white egg shell with large spots, egg shell with large spots, violet egg shell with small spots and beige egg shell with small spots were 0.0449mm, 0.0431mm, 0.0398mm, and 0.0397mm, respectively. 4. Negative correlation coefficient was found between egg shell thickness ana egg shell membrane thickness, but it was not significant. 5. Comparing quail egg with fowl egg, the thickness of quail egg shell was much thinner than that of fowl egg, but egg shell membrane thickness was similar between the two.

• Korean Chemical Engineering Research
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• v.50 no.4
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• pp.713-717
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• 2012

Approximately forty proteins in egg white have been widely studied for their functional properties. To develop a procedure of separation for pure and non-altered proteins from egg white, purification study was conducted to isolate lysozyme, ovotransferrin, and ovalbumin. Ion exchange cartridge can selectively separate proteins from egg white, and reversed-phase HPLC (RP-HPLC) could identify separated proteins. Proteins in egg white were purified by HI trap ion exchange cartridge SP and Q with buffers pH 8.0 and 5.2. C18 column (Phenomenex, USA) was used for RP-HPLC analysis and isocratic mobile phase was used with acetonitrile (ACN)/distilled water (DW)/trifluoroacetic acid (TFA) in the ratio of 50/50/0.1. Comparing the retention times of standards in RP-HPLC experiments showed that ovotransferrin, ovalbumin, and lysozyme in egg white were eluted successively in the RP-HPLC column after the pretreatment in SP and Q ion exchange cartridges.

• Culinary science and hospitality research
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• v.24 no.2
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• pp.103-111
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• 2018

The objectives of the present study were to examine the antioxidant activity of autofocusing fractions from egg white protein hydrolysates and obtain higher antioxidant peptide fraction, which could be applied to the food model system. Alkaline protease hydrolysate of egg white protein exerted higher antioxidant activities than other protein hydrolysates and were fractionated on the basis of the amphoteric nature of sample peptides by preparative isoelectric focusing without toxic solvents and reagents, which is termed autofocusing. Neutral and basic fractions showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the acidic fractions. The acidic and neutral fractions showed higher hydroxyl (OH) radical scavenging activity and oxygen radical absorbance capacity (ORAC) values than the basic fractions. The acidic fractions showed higher metal chelating activity than basic fractions. Antioxidant activities of some autofocusing fractions except for ORAC showed higher compared to the crude hydrolysate. These results suggest that peptides fractions from egg white protein are effective antioxidant, and that autofocusing could be useful to increase antioxidant activity for application to food system.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.2
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• pp.157-165
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• 2002

Genetic variations of seven egg white protein loci in 1,112 samples from eight Asian countries (Yunnan province of China, Mongolia, Nepal, Vietnam, Laos, Thailand, Myanmar, Indonesia) and 360 samples from two improved breeds (Isa Brown, Boris Brown) were investigated by using starch gel and polyacrylamide gel electrophoresis. Five egg white protein loci (Ov, $G_3$, $G_2$, $G_1$ and $Tf_{EW}$) were found to be polymorphic in Asian native chicken populations. The proportion of polymorphic loci ($P_{poly}$) and average heterozygosity ($\bar{H}$) of Asian native populations varied from 0.143 to 0.714 and 0.014 to 0.225, respectively, and were higher than those of improved breeds. The subdivision index ($G_{ST}$) value among 18 native chicken populations in Asia is lower (0.0827) than among improved chicken populations (0.1693). This value means that the degree of subdivision among Asian native populations is lower than among improved breeds and gene constitutions among populations in Asia are similar.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1364-1368
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• 2009

Lysosomes, as a cell organelle type, are safe biological control agents that may be possible replacements for chemical antimicrobial agents because they are simply isolated from egg white. In this study, it was found that the lysosomes isolated from egg white exhibited pH-dependent antimicrobial activity, with the optimal activity found at pH 6.0. The efficiency of lysosomes in inhibiting bacterial growth and activity was evaluated over a 12-h treatment period. Seven different microorganisms were used as bacterial strains, and the lysosomes showed a significant antimicrobial effect against all strains. In addition, the antimicrobial activity was maintained for 100 days, and there did not appear to be any resistance of E. coli to the lysosomal activity up to the eighth culture. However, the lysosomes did not affect the viability of mammalian cells, suggesting the biocompatibility of lysosomes. These highly effective lysosomes have a bright future in the application of novel antimicrobial sources as a cell organelle type.