• Korean Journal of Ichthyology
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• v.27 no.2
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• pp.95-103
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• 2015

Egg development and early life history of the Korean spine loach, Cobitis tetralineata, were investigated in the present study. Adult fish were sampled using spoon nets at the Seomjin River in Jeongsan-ri, Bokheong-myeon, Sunchang-gun, Jeollanbuk-do, Korea, July 2013. Eggs were obtained after injecting Ovarprim into females. Eggs were then artificially fertilized using the Dry method in the laboratory. Mature eggs were slightly adhesive and transparent with grey coloring, and measured $1.04{\pm}0.03mm$ ($mean{\pm}SD$) in diameter. Hatching of the embryo occurred approximately 56 hours after fertilization at $25^{\circ}C$ of water temperature, and the average size of newly hatched larvae was about $4.6{\pm}0.11mm$ in total length. At fifth day after hatching, the larval full length reached $6.8{\pm}0.28mm$ on average and their yolk sac had been completely absorbed. At 17th day after hatching, larva entered the juvenile stage and reached $9.8{\pm}0.50mm$ in total length. At 100th day after hatching, the formation of Gambetta's zone of four line was complete and juveniles were similar in general appearance to adults, and they averaged $34.7{\pm}5.77mm$ in total length.

• Korean Journal of Ichthyology
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• v.30 no.1
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• pp.18-26
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• 2018

We investigated to egg development and early life history of Tribolodon brandtii in the Korean peninsula. The fertilized eggs of the T. brandtii were slightly adhesive and separated with a yellow yolk and no oil globule, and they was an average of $2.4{\pm}0.2mm$ in diameter. The hatching of the embryo took place in about 60 hrs after fertilization under water temperature of $20{\pm}1^{\circ}C$ and the newly hatched larvae were average $6.4{\pm}0.4mm$ in total length. At 10 days after hatching, they were average $11.7{\pm}0.2mm$ in total length and their yolk sac were completely absorbed. At 46 days after hatching, they became to juvenile stage and reached $19.6{\pm}2.7mm$ in total length and all their fin rays were formed. At 125 days after hatching, the juvenile were $54.9{\pm}15.8mm$ in total length and their body shape were similar to the adult fish. At 236 days after hatching, they fish were $54.9{\pm}15.8mm$ in total length and their body color were similar to the adult fish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.4
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• pp.323-328
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• 1982

Kareius bicoloratus (Basilewsky) is one of the commonly found right-eye flounders and widely distributed in the coastal waters of Korea and Japan. On December 11,1980, the ailthors carried out an experiment to obtain a large number of fertilized eggs from wild adult fish caught by a trawler. The fish were obtained from Maisaka fish market, Shizuoka Prefecture, Japan. The egg is pelagic, spherical in shape and measuring 1.014-1.04 mm in diameter. The yolk as well as the egg capsule is colorless and transparent, and contain no oil globules. The hatching took place in 73 hr 45 min after fertilization at the water temperature $8.0-9.5^{\circ}C$. Newly hatched larvae are 3.09-3.146 mm in total length, with the anus situated in the middle of the body. The marginal fin does not have Pigment cells and myotome number is 17+20=37. Within one day after hatching, the larvae attained 3.77 mm in total length, and there appeared three or four melanophore on yolk sac. When the larvae attained 3.96 mm in total length, melanophores began to appear on the eye ball. Two days after hatching, the larvae attained 4.05 mm in total length, most of yolk material was absorbed, and the caudal fin began to grow at the terminal part of the notochord. When the larvae attained 4.21 mm in total length, mouth and eyes began to move. After 3 days, the larvae attained 4.342-4.394 mm in total length, alimentary canal differentiated, melanophores appeared on the lower jaw and posterior part of the fin membrane. When the larvae attaind 4.576 mm in total length, marginal line of dorsal fin membrane became concave.

• Korean Journal of Ichthyology
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• v.25 no.4
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• pp.200-207
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• 2013

We investigated developmental stages of egg and early life history of the Korean indigenous fish, Korean Chub Zacco koreanus from the South Han River in 2011 for phylogenetic study and conservation of this species. Eggs of Zacco koreanus were artificially fertilized by the dry method in the laboratory. The fertilized eggs were demersal, almost spherical in shape, transparent and unpigmented, with a pale yellow yolk, and no oil globule and average $3.09{\pm}0.07$ mm (n=10) in diameter. The hatching of the embryo took place in about 68 hrs after fertilization under water temperature of $20.0{\sim}23.0^{\circ}C$ and the newly hatched larvae were average $10.30{\pm}0.40$ mm (n=10) in total length (TL). Six days after hatching, the larvae grew up to $16.12{\pm}0.42$ mm (n=8) in TL and york sac absorption, mouth and anus opening were shown postflexion larvae stage. 17 days after hatching, most of fin-rays appeared at $18.21{\pm}0.38$ mm (n=6) in TL and brown spot appeared on the abdomen. 27 days after hatching, the larvae were brought up to $20.01{\pm}1.12$ mm(n=5) in TL and all their fin-rays were formed. 120 days after hatching, the larvae (juvenile) were $23.29{\pm}3.12$ mm (n=10) in TL and their body shape and color pattern were similar to the adult fish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.1
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• pp.114-120
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• 1998

The spawning behavior, development of eggs and larvae of the Korea freshwater goby, Rhinogobius brunneus (Temminck et Schlegel) were studied. The eggs were spawned as a one-layer mass, hanging from the underside of a small pebble, and guarded by one male. The eggs were elliptic, about 1.48 mm in length and 0.65 mm in breadth, with a round top and a somewhat flat base with glutinous fibers. Hatching in the indoor tank with $17.0^{\circ}C$ in mean water temperature started from the 146 hours after fertilization. In the late developing stages, the embryo moved and freely changed its head to face the free tip of the chorion (normal egg) or to the basal end with a boundle of adhesive filaments (agrippa egg). Newly-hatched larvae were $3.10\~3.30$ mm in total length (mean: 3.22 mm), and mouth and anus were not yet open. Melanophores were present on the air- bladder, around the anus, and on the ventral part of the caudal region. The larva $3\~4$ days old transformed to postlarval stage, and they were $3.30\~3.85$ mm in total length (mean: 3.60 mm). As yolk sac and oil globules werw nearly absorbed, mouth and anus were open, and they fed rotifers actively. In $20\~22$ days after hatching the larvae grew to 5.85 mm in 71, and the caudal notochord flex at $45^{\circ}$.

• Korean Journal of Ichthyology
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• v.31 no.1
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• pp.30-38
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• 2019

Egg development and early life history of the Korean endemic sand spine loach, Cobitis nalbanti, were investigated in the present study. Adult fish were sampled using spoon nets at the Yeongsan River in Seongam-ri, Bukha-myeon, Jangseong-gun, Jeollanam-do, Korea, June 2011. Eggs were obtained after injecting Ovarprim into females. Eggs were then artificially fertilized using the dry method in the laboratory. Mature eggs were transparent and slightly adhesive with light yellowish coloring, measured $0.99{\pm}0.03mm$ ($mean{\pm}SD$) in diameter. And number of spawned eggs were $1,527{\pm}410$ per individual. Hatching (50%) of the embryo occurred 52 hours after fertilization a water temperature of $25^{\circ}C$, and the average newly hatched larvae size was about $4.2{\pm}0.22mm$ in total length. At fifth day after hatching, the larval total length reached $6.0{\pm}0.34mm$ on average and their yolk sac had been completely absorbed. At fifteen day after hatching, larva entered the juvenile stage and reached $10.8{\pm}0.45mm$ in total length. At 100th day after hatching, the formation of Gambetta's zone of four dotted line was complete and juveniles were similar in general appearance to adults, and they averaged $41.1{\pm}2.95mm$ in total length.

• Korean Journal of Ichthyology
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• v.33 no.3
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• pp.167-176
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• 2021

This study was carried out to clarify the egg, larvae and juveniles development of Abbottina springeri. For the experiments, the matured adults were collected at the Seowon-cheon, Dangjin-si, Chungcheongnam-do, in Korea. The amount of spawning of female A. springeri was about 1,225~2,100 (1,662±437, n=10). The fertilized eggs were circular in shape and 1.05~1.13 (1.08±0.02, n=30) mm in diameter. The hatching time was required 72 hours to 80 hours after fertilization under water temperature of 22℃. The newly hatched larvae were 2.10~2.23 (2.16±0.04, n=10) mm in total length and had egg yolk in the abdomen but the mouth was not opened. At 5 days after hatching, the preflexion larvae were 3.19~3.30 (3.24±0.03, n=10) mm in total length and the most of yolk-sac was absorbed. At the 15 days after hatching, the flexion larvae were 4.97~5.30 (5.13±0.12, n=10) mm in total length and the tip of the caudal fin was began to bend upward. At the 25 days after hatching, the postflexion larvae were 8.97~9.60 (9.44±0.16, n=10) mm in total length and the tip of the caudal fin was bent at 45°. At the 35 days after hatching, the juvenile were 12.0~13.5 (12.7±0.53, n=10) mm in total length and all fin-rays (iii7 dorsal fin, iii6 anal fin, i7 ventral fins) were reached a constant number of each part.

• Korean Journal of Ichthyology
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• v.36 no.1
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• pp.10-19
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• 2024

This study investigated the early life history of Hemibarbus labeo from in Wicheon, stream Nakdonggang-River, and compared their characteristics with closely related species. In April 2021, egg formation and development of autonomous fish were observed in fertilized eggs collected at four spawning sites. The size of the fertilized egg was 1.93~2.39 (average 2.22±0.15, n=30) mm. The water temperature was 22.2~24.1°C, and the hatching time took 109~115 hours. Newly after hatching, the total length of the yolk-sac larvae was 7.50~8.80 (average 7.99±0.46) mm, and the mouth and anus did not develop and had difficulty in being yolk. 6 days after hatching, the preflexion larvae were fed with a total length of 9.49~10.2 (9.78±0.23, n=30) mm. 10 days after hatching, the flexion larvae was 9.97~11.9 (10.7±0.72, n=30) mm in total length, and the tail of the vertebrae began to bend. 20 days after hatching, the postflexion larvae was 12.6~15.2 (13.9±0.77, n=30) mm in total length, and the tailbone was completely bent to 45°. 29 days after hatching, the total length of the juvenile was 16.9~19.8 (18.1±0.91, n=30) mm, and the number of fins reached an integer with 10 dorsal fins, 9 anal fins, and 7 ventral fins. The distribution of melanophore, such as the head, the center of the body, and the upper part of the fin, was different from that of the allied species during the postflexion larvae period, so the morphological characteristics could be distinguished.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.1
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• pp.24-32
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• 1987

Bluegill, Lepomis macrochirus RAFINESQUE, has bred widely in the various districts of Korea since the Office of Fishery transplanted it into our country from Japan in December, 1969. On August 17, 24 in 1985, bluegill were caught in the reservoir of Habuk-Myon, Yangsan-Gun, $Ky\breve{o}ngsangam-Do$, Korea. They spawned in the rearing aquarium on July 13 and August 2 in 1986. The eggs of this species are adhesive and demersal. The size of the egg diameters were varied from 1.18 to 1.30 mm. Hatching took place in 40 hours after fertilization at the water temperature of $24.3-25.4^{\circ}C$. The newly botched larvae were 3.75-4.05 mm in total length possessing yolk sac, and 29-30 mytomes. Many melanophores were evenly distributed on the entire body. Ten days after hatching, the postlarvae attained 6.05-6.35 mm in total length. The yolk sac was completely absorbed and the width of the pectoral finfold had reached the maximum size. One month fifteen days after hatching, the juvenile attained 25.20 mm in total length.

• Journal of Korean Ophthalmic Optics Society
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• v.7 no.1
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• pp.39-43
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• 2002

This study was investigated the anti-angiogenic activities of Cnidium officinale Makino and Tabanus bovinus by using Chorioallantoic membrane (CAM) and rat cornea. First experiment, the fertilized chicken eggs were kept in a humidified egg incubator at $37^{\circ}C$. After 4 days incubation, about 3 ml of albumin was aspirated from eggs with an hypodermic needles through a small hole drilled at the narrow end of the eggs, and the shell membrane on the floor of the air sac was peeled away. Embryos with chorioallantois of 3~5 mm in diameter were employed for the assay of antiangiogenic activity. Retinoic acid was used as a positive control for this experiment. After 48 h of treatment. branching pattern of blood vessels below coverslip containing retinoic acid ($10{\mu}g/egg$) was dramatically decreased. A simiar angiogenic inhibition was observed in the CAM treated with $50{\mu}g/egg$ of Cnidium omcinale Makino and Tabanus bovinus extracts. Second experiment, rat corneal neovascularization was induced by suturing (one stitch) the cornea with 10-0 nylon, and terramycin was applied on the cornea for I week to prevent corneal inflammation. In the cornea of rats untreated with herbal extracts, numerous vessels were usually seen invading the cornea by day 2 or 3 after suture, and reaching the lesion area within 5~6 days. Corneal neovascularization was gradually increased and peaked at 3 weeks. In contrast to this, herbal extracts conspicuously inhibited the angiogenesis, Oral administration of herbal extracts (20 mg/kg body weight/day) for 4 weeks significantly inhibited the rat corneal angiogenesis induced by suture, and the length of blood vessels in herbal medicine-treated rat cornea was conspicuously lower than that in control animals. A similar phenomenon was also observed in the rat cornea treated with thalidomide (200 mg/kg body weight/day). These findings indicate the anti-angiogenic properties of Cnidium officinale Makino and Tabanus bovinus, suggesting that these properties may be one of the pharmacological mechanisms underlying the anti-tumor and anti-metastatic activities of herbal extracts tested in this study.