• Title/Summary/Keyword: egg sac

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Effect of Bacillus spp. Having Gelatin Decomposing Activity on Root-knot Nematode, Meloidogyne incognita on Pepper (젤라틴 분해력이 있는 Bacillus균의 고추 고구마뿌리혹선충에 미치는 영향)

  • Kim, Jin Han;Park, Chan Sun;Kim, Young-Ho;Kim, Yu Ri;Lee, Seung Woong;Rho, Mun Chual
    • The Korean Journal of Pesticide Science
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    • v.20 no.4
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    • pp.375-379
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    • 2016
  • This study investigated the decomposing activities on gelatin and egg sac of root-knot nematode (Meloidogyne incognita). Ten Bacillus isolates (KRB-1~10) isolated from the soils of Mt. Naejang. Among them, KRB-5, 9, and 10 showed decomposing activities, and identified as Bacillus subtilis KRB-5, Bacillus amyloliquefaciens KRB-9, and KRB-10 by 16S rRNA sequence analysis, respectively. Under pot experiments using pepper, 100-fold diluted culture broth of three isolates reduced the number of egg sac on roots collected 60 days and 90 days and increased the growth of aerial part compared with the group only treated with Meloidogyne incognita. Especially, the group treated B. subtilis KRB-5 was superior in the growth of pepper. These results suggest that the B. subtilis KRB-5 can be used to control the root-knot nematode on pepper.

Embryogenesis in the Octopus minor (낙지(Octopus minor)의 배 발생)

  • Kim, Dong-Soo;Kim, Jae-Man
    • Development and Reproduction
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    • v.10 no.2
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    • pp.135-140
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    • 2006
  • The small octopus(Octopus minor), a species in the cephalopod class spawns the fewer eggs compared with its relatives such as Octopus vulgaris and Octopus ocellatus and exerts special efforts in nursing eggs hiding in the deep cave beneath the mud flat. Correspondingly, its embryogenic process had been hardly observed. In this study, we induced the small octopus to spawn its eggs in the artificial spawning tube and to nurse the developing embryos under the periodical observation. The early embryogenic change in the fertilized eggs was appearance of blastodisc on the animal pole at 4 days after spawning. A mass of rudiments of several organs developed in the middle of the yolk sac at 28-th day and it moved gradually toward the animal pole. At 38 days after spawning, two eyes appeared on the head and the trunk with heart developed on the apical part of the yolk sac. At 45-th day of embryonic development, the trunk and head occupied a half of the egg capsule and consequently the yolk sac was reduced to a half of its original size. Two eyes became prominent at the bottom of the trunk with several spots at day 60. Embryonic development was completed at about 80 days after spawning and the arms stuffed the lower half of egg capsule. The young fry squeezed out of the egg capsules with their long and slender arms first and mantles later. Soon after hatching, it swam actively and squirted ink.

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Five Cases of Diphyllobothrium latum Infection (광절열두조충감염 5예)

  • 이순형;서병양
    • Parasites, Hosts and Diseases
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    • v.21 no.2
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    • pp.150-156
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    • 1983
  • Five cases of Diphyllobothrium latum infection were proved by collection of worms after treatment with bithionol, niclosamide or praziquantel, during 1975~1983. The patients were 4 males and one female aged 10~64 years residing in Seoul or Wando-gun, Chollanam-do, Korea. All of them remembered the history of eating several kinds of raw marine fish and 2 persons said they ate the raw mesh of perch, Lateolabrax japcnicus. Three amcnE them experienced abdominal pain, 2 natural discharge of a chain of segments, but none revealed any sign of anemia. The average egg size in stool varied $59~67{\times}41~48{\mu\textrm{m}}$ according to cases. The eggs were characterised by ovoid to elliptical shape, terminal operculum, and distinct abopercular protuberance. One naturally discharged segment (30cm) and 4 complete strobilae (320~680cm) with scolices obtained after treatments were examined. The morphological characters of proglottids such as rosette-form uterus with 3~6 loops, vaginal pore included in the cirrus sac, separated seminal vesicle from cirrus sac, etc. were all compatible with D. latum. These are the 7th~11th cases of D. latum infection proved by worms in Korea.

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Effects of Incubation Temperature on Egg Development, Hatching and Pigment Plug Evacuation in Farmed Siberian Sturgeon Acipenser baerii

  • Park, Chulhong;Lee, Sang Yoon;Kim, Dong Soo;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
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    • v.16 no.1
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    • pp.25-34
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    • 2013
  • Early ontogenic development in Siberian sturgeon Acipenser baerii was documented and the effects of different temperatures on embryonic and prelarval development were examined. Photograph-assisted data on morphogenesis in Siberian sturgeon prolarvae agreed well with published descriptions of their ontogeny and ecological behaviors, although certain aspects of differentiation, such as gill covering and scute development, could be rearing condition-sensitive. The present study provides the first characterization of the transient development of teeth during early larval stages; the pattern was congruent with the transition of prolarvae to exogenous feeding. From examinations of embryonic and prelarval development under different temperature conditions ($12-24^{\circ}C$), developmental speed was inversely related with temperature. Overall, hatchability was higher and hatching events were more synchronized at $20^{\circ}C$ than at lower temperatures. After hatching, similar patterns of temperature-dependency were observed in yolk sac absorption and the evacuation of the pigment plug. Our results suggest that the incubation of Siberian sturgeon embryos and prolarvae at temperatures close to $20^{\circ}C$ would be advantageous in hatcheries, based on reductions in the duration and uniformity of egg and prolarval developmental stages.

Studies on the Toxicity of Benzophenone in the Developing Chick Embryo (계배 발생과정에서 benzophenone의 독성에 관한 연구)

  • Yoo, Min;Kim, Su-Won
    • Journal of Life Science
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    • v.19 no.9
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    • pp.1309-1313
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    • 2009
  • Endocrine disruptors are chemicals which can be found in our normal daily lives. Chemicals such as bisphenol A, DDT, benzophenone and phenylphenol can be easily ingested through plastic food containers and pesticides. Endocrine disruptors can be very harmful and toxic because they disrupt the normal function of the endocrine system. It has been reported that endocrine disruptions can cause fatal strikes in the cardiovascular, reproductive, and central nervous systems, and other parts of the body. Therefore we examined if benzophenone as an endocrine disruptor inhibits development in or induces malformation of chick embryos. Chick embryos which received a single injection of benzophenone ($1{\mu}g$/egg $\sim$ $500{\mu}g$/egg) via the yolk sac at designated times (6, 9, 12, 15, 18 and 21 days after incubation) were investigated. Body weight reduction was observed in middle doses ($40{\mu}g$/egg $\sim$ $60{\mu}g$/egg). High mortality rates and teratogenic signs such as abnormal wry beak and abnormal eyeballs were seen in high doses ($80{\mu}g$/egg $\sim$ $500{\mu}g$/egg). In conclusion, it is suggested that benzophenone induces malformation of chick embryos and seriously inhibits development.

Evaluation of efficacy of Mycoplasma gallisepticum 6/85 live vaccine (닭 Mycoplasma gallisepticum 6/85 생균 백신의 효능 평가)

  • Yoon, Hee-Jun;Kang, Zheng-Wu;Jin, Ji-Dong;Shin, Eun-Kyung;Jeong, Yong-Hoon;Jeong, Ji-Hye;Hahn, Tae-Wook
    • Korean Journal of Veterinary Research
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    • v.46 no.3
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    • pp.207-214
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    • 2006
  • Mycoplasma gallisepticum (MG) continues to persist in many commercial layer farms in Korea,resulting in losses in egg production. Bacterins and live attenuated vaccines have been used for the prevention of losses caused by MG. One of these attenuated vaccines, MG 6/85 vaccine has been reported to be safe and efficacious in layers. However, MG 6/85 vaccine has not been evaluated for its safety and its efficacy in any commercial layer in Korea. Six-week-old specific pathogen-free (SPF) chickens were vaccinated with MG 6/85 vaccine by aerosol and were challenged with virulent MG R strain at 4 weeks after vaccination. The vaccinated group was able to resist challenge into the air sacs because the vaccinated group showed much less air sac lesion compared with the unvaccinated group. Each of two commercial layer farms was divided into vaccinated and unvaccinated groups. For each vaccinated gorup, MG 6/85 vaccine were sprayed at 17 week old on farm A and at 15 weeks old on farm B. Hen-day egg production, Hen-housed eggs, egg weight, mortality were evaluated until 50 week after vaccination.Compared with the unvaccinated group in each farm, the vaccinated group showed higher average egg production and egg weight, and higher hen-housed number. Results of this study are in agreement with other previous reports which demonstrated that MG 6/85 vaccine favorable effect on performance in commercial layers.

Studies on the Morphological Characteristics and Life History of the Chili Fish, Cultriculus eigenmanni (Jordan et Metz) (1) On the Egg Development and the Fry in the Larval Stage (치리(Cultriculus eigenmanni)의 형태 및 생활사에 관한 연구 I. 난발생과 부화자어에 대하여)

  • KIM Eul-Bae;KIM Young-Gill
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.151-156
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    • 1984
  • The Chili fish, one of the most common species of the freshwater fishes, is distributed in the river basins of the western coast of Korea. However, any studies on this fish, especially its development or life history, have not been made so far. The authors, therefore, carried out an experiment to reveal some characteristics of egg develoment and fry in this fish. For accomplshing this study, we caught some adult Chili fish by means of a casting net at raceway in the vicinity of Kunsan in June and July, 1983; then, we injected 0. 1 cc Gonadotrophic hormone 1,000 unit solution (Puberogen) in order to induce the spawning on the abdominal cavity of male and female respectively, Approximately 12 hours after injection, the artificially fertilized eggs by using of the dry method and the naturally fertilized eggs were observed simultaneously in the aquarium ($30{\times}50{\times}70cm$). The fertilized eggs, in spherical shape, were sperative, demersel, adhesive nature and $1.38{\sim}1.59\;mm$ in diameter. The light yellow yolk was opaque and $1.17{\sim}l.27\;mm$ in diameter. The hatching of eggs took place at 29.5 hours after fertilization under the condition of $23.5{\sim}25.0^{\circ}C$ water temperature. Basically there were not found so many different aspects in comparison with the common species of Cyprinidae in the development process of eggs : however, there were a few noticable characteristics, that is, pigment cells, eye balls and blood circulation are invisible, or the velocity of egg development is rapid, seemed similar to the Loach's egg. Newly hatched larvae with the yolk sac (2.39 mm in size) were $3.87{\sim}3.99\;mm$ in total length and their myotome number was expressed as 28+15=43. About 20 hours after hatching, a caudal and a pectoral fin as well as an anus began to orgin. In addition, a few malanophore showed on the upper regin of the yolk sac of the larvae whose size was 4.73 mm in total length. The larvae developed about 5.02 mm in total length, and the median fin fold in the abdomen extended forward to two thirds or beyond of the yolk sac at 50 hours after hatching. Simultaneously, the eye balls and mouth also appeared. After 3 days, the yolk sac nearly disappeared, and the larvae (5.4 mm in total length) with perfect eye balls began to swim for themselves for the first time. Also, the specialization between the gill arches and the ailmentary canal, the development of each fins except dorsal fin as well as the blood circulation obviously distinguished in this stage. The larvae whose total length was 5.68 mm was very brisk: also, the development of the dorsal fin rudiment, nearly perfect formation of each organs and the function of the upper and lower jaws were noticeably observed 5 days after hatching.

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Studies on the teratogenicity of folpet in the developing chick embryo (계태아를 이용한 농약(folpet)의 기형독성 연구)

  • Lim, Yoon-kyu;Heo, Gang-joon;Lee, Yong-soon
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.375-379
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    • 1994
  • A teratogenicity test of 'folpet' was carried out in the developing chick embryos to investigate and validate the safety of rural environmental hazardous materials. Folpet was administered to chick embryos' yolk sac at a rate of 0.1mg and 0.01mg per SPF eggs at 96 hours of incubation. The morphological changes were examined. Fertility ratio of SPF eggs used was 94.9%. Hatching rate of untreated control group was 74.4% and the group dosed with 100ul of corn oil into the yolk sac was 70.0%. The $LD_{50}$ of folpet was 0.663mg/100ul/egg. After hatching, mean body weight, body length, claw length and beak length of high and low dose administered groups were not significantly different from untreated and vehicle control group. There was no abnormal appearence in all the groups. Therefore it seems that, within the doses applied, folpet dose not induce potential teratogenicity in the developing chick embryos.

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Spawning Behavior, Egg Development, Larvae and Juvenile Morphology of Hyphessobrycon eques (Pisces: Characidae) Characidae Fishes

  • Park, Jae-Min;Kim, Na-Ri;Han, Kyeong-Ho;Han, Ji-Hyeong;Son, Maeng-Hyun;Cho, Jae-Kwon
    • Development and Reproduction
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    • v.18 no.4
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    • pp.241-249
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    • 2014
  • Hyphessobrycon eques is a famous fish for ornamental fish market and aquarium. They are inhabit in regions of Amazon and Paraguay River basin. Serpae fishs were investigated 2-3 males are chased to female, and then males attempted to simulate the females abdomen. After fertilization, eggs were kept in incubators at $28^{\circ}C$. The fertilized eggs had adhesive and demesal characteristics and had a mean diameter of $0.92{\pm}0.01mm$. Larvae hatched at 16 hrs post fertilization. The hatched larvae averaged $2.90{\pm}0.16mm$ in total length ($L_T$). Complete yolk sac resorption and mouth opening occurred on the third day post hatching. At 45 days post hatching, the larvae were $12.5{\pm}1.60mm$ $L_T$ and had reached the juvenile stage.