• Korean journal of applied entomology
• /
• v.32 no.4
• /
• pp.450-456
• /
• 1993

We investigated effects of a topical treatment of methoprene(0.5-5.0$\mul$/egg), a juvenLle horm mane analogue, on embryo development in the gypsy moth, Lymantri$\alpha$ dispar. Methoprene lowered egg hatching rate, and also reduced the mean wet weights of hatched 1st instar larvae w with the most effect shown at the highest concentration. The differences in protein(p < 0.01) and carbohydrate(p < 0.05) contents between control and methoprene(5$\mul$/ egg) treatment g groups were observed during embryo development.

• Korean journal of applied entomology
• /
• v.37 no.2
• /
• pp.159-162
• /
• 1998

Effect of temperature on oviposition and developmental period of Oulema oryzae K. on riceplant were investigated. With the given set of temperatures of 15, 20, 23, 25, and 28"C, developmentalperiod from egg to adult emergence was shorten as temperature increased. Average number of eggs perfemale 0. oryzae increased as temperature increased from 15$^{\circ}$C to 23"C, then decreased at 25$^{\circ}$C and 28"C. Based on this result, developmental threshold temperatures for egg was estimated to be 6.4"C. Totaleffective temperatures above the thresolds required for hatching were estimated to be 75.8 degree-daysfor egg. It seemed that the optimum temperature for oviposition of Oulema oryzae on rice plant was 23"C.a oryzae on rice plant was 23"C.quot;C.

• Korean journal of applied entomology
• /
• v.61 no.3
• /
• pp.435-447
• /
• 2022

The soybean podborer, Matsumuraeses falcana (Lepidoptera: Tortricidae), is one of important pests in soybean crop. In the purpose of forecasting population dynamics of M. falcana, we investigated the effects of temperature on development of each life stage, adult longevity and fecundity of Matsumuraeses falcana at seven constant temperatures of 10, 13, 19, 22, 25, 28, and 31℃. Eggs hatched successfully at all temperature subjected. M. falcana developed from egg hatching to adult emergence at the tested temperatures except 10, 13, and 31℃. The developmental period of each life stage and adult longevity of M. falcana decreased as temperature increased. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of M. falcana were estimated by linear regression as 10.2℃ and 492.04DD, respectively. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. TL and TH from egg hatching to adult emergence using SSI model were 16.7℃ and 29.1℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of M. falcana was 12.4℃. We constructed the adult oviposition model of M. falcana using adult survivorship and fecundity. Temperature-dependent immature development and adult oviposition models will help constructing the population model of M. falcana and developing the strategies of integrated pest management in soybean fields.

• Korean journal of applied entomology
• /
• v.61 no.4
• /
• pp.577-590
• /
• 2022

Ostrinia scapulalis is one of important pests in leguminous crops, especially red bean. In order to understand the biological characteristics of the insect, we investigated the effects of temperature on development of each life stage, adult longevity and fecundity of O. scapulalis at eleven constant temperatures of 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, and 36℃. Eggs and larvae successfully developed next life stage at most temperature subjected except 7, 10 and 13℃. The developmental period of egg, larva and pupa decreased as temperature increased. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of O. scapulalis were estimated by linear regression as 13.5℃ and 384.5DD, respectively. TL and TH from egg hatching to adult emergence using SSI model were 19.4℃ and 39.8℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of O. scapulalis was 20.4℃. Adults produced viable eggs at the temperature range between 16℃ and 34℃, and showed a maximum number, ca. 416 offsprings, at 25℃. Adult models including aging rate, age-specific survival rate, age-specific cumulative oviposition, and temperature-dependent fecundity were constructed, using the temperature-dependent adult traits. Temperature-dependent development models and adult oviposition models will be useful components to understand the population dynamics of O. scapulalis and will be expected using a basic data for establishing the strategy of integrated pest management in leguminous crops.

• Korean Journal of Ecology and Environment
• /
• v.33 no.3 s.91
• /
• pp.282-294
• /
• 2000

The reproductive ecology and the early life history of Macropodus chinensis were investigated in aquarium. Mature male made the bubble nest and spawned with wrapping the female and reverse posture. The parental male protected the offspring until the larvae depart the bubble nest. Egg productivity and egg hatching rate were the highest at water temperature in $28^{\circ}C$ and $26^{\circ}C$ respectivity than any other artificial temperature. The eggs were buoyant, globular and 0.95${\sim}$1.05 mm in diameter. Cleavage was progressed at intervals of 15 minutes. Eggs hatched in 42${\sim}$44hours after fertilization and the newly hatched larvae were 3.0${\sim}$3.2 mm in total length. The lawae were 4.5${\sim}$5.4 mm in 4${\sim}$5 days after hatching and fed on the food with dispersion from the nest. In 40${\sim}$45 days after hatching, all fin rays completely developed, and juveniles reached 18.2${\sim}$23.5 mm in total length. In 90${\sim}$110 days after hatching, body from of young fishes were similar to adult with 37.4${\sim}$48.2 mm and represented secondary sexual characters longer than 45.0 mm in total length, and about 120 days, fishes began spawning(water temperature for ontogenesis: $26.0{\pm} 1^{\circ}C$).

• Development and Reproduction
• /
• v.22 no.3
• /
• pp.289-295
• /
• 2018

Large quantity of eggs fail to be fertilized and many of fertilized eggs are unable to hatch in the eel, Anguilla japonica. Larvae of eel absorb egg yolk up to 8 days after hatching but the majority of hatched larvae die before they reach the stage of first feeding in this species. Genes of key enzymes for yolk processing (cathepsin B, D, L and lipoprotein lipase - abbreviated as ctsb, ctsd, ctsl and lpl, respectively) could be associated with egg quality. In this study, we investigated differences in the expression of these genes between floating eggs and sinking eggs, and also the relationship between the gene expressions of the enzymes and fertilization rates in the fertilized eggs obtained from artificially matured female eels. Expressions of yolk processing enzyme genes did not show significant difference between floating and sinking egg groups. Expression of ctsb decreased when fertilization rate was high. Expression of ctsd, ctsl and lpl, however, did not show any significant differences. These results suggest that ctsb expression could be an indicator of egg quality, and that some proteins prone to be digested by ctsb could be very important in the process of fertilization and normal cleavage in this species. Further study should identify these critical proteins to improve our understanding on the quality of fish eggs.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.35 no.2
• /
• pp.135-139
• /
• 2002

In order to obtain the basic information for the seedling production of surf clam, Spisula sachalinensis, sperm motility and optimal method for fertilization were investigated. Sperm concentration of S. sachalinensis milt was$ 2.02{\times}10^{10}\;cell/mL$ and approximately $96.0\%$ of sperm showed forward movement after exposure to seawater. When sperm and eggs obtained by incision method were fertilized in 1 hour and 4 hours, respectively, high fertilization and hatching rate were achieved. The optimal sperm concentrations and egg density for fertilization and hatching were 10$\~$100 inds./egg and 100$\~$200 inds./mL sea water, respectively.

• Journal of Aquaculture
• /
• v.17 no.3
• /
• pp.180-186
• /
• 2004

This study was carried out to obtain the fundamental data for the mass seedling production of grunt, Hapalogenys nitens in terms of the natural spawning and some characteristics of the eggs spawned. The wild grunt were reared at indoor tanks for three years. The adults spawners were 34.0∼44.0 cm (38.6$\pm$4.0 cm, n=7) in total length, 1.00∼2.23 kg (1.62$\pm$0.50 kg, n=7) in body weight. Spawning were observed 9 times from September 22 to October 1, 2000 and 37 times from August 22 to October 3, 2001, with a water temperature range of 19.8$\pm$28.5$^{\circ}C$. The total number of eggs collected was 2.29${\times}$10$^{7}$ (1.7${\times}$10$^{3}$/ml). The relative proportion of floating eggs to total eggs was 41.7%. The fertilization rate of floating eggs was ranged between 85.0 and 99.9% and the hatching rate was ranged between 2.9 and 93.0%. Fertilized eggs were buoyant and spherical in shape, and were 0.85∼0.98 mm in diameter. Each egg contained 1-5 oil globules which were, 0.18∼0.25 mm in diameter. The incubation time from fertilization to blastodisc formation was 10 minutes, to blastula was 3 hours, and to the hatched larvae at 26$^{\circ}C$ was 20 hours 30 minutes. The newly hatched larvae attained total length of 1.81$\pm$0.18 mm. The time required from fertilization to hatching was 31∼34 hours at 23$^{\circ}C$ and 17∼20 hours at 29$^{\circ}C$.

• Korean Journal of Poultry Science
• /
• v.31 no.4
• /
• pp.299-306
• /
• 2004

The objective of this study was to investigate the influence of egg storage, broiler breeder age, and the change of egg weight during incubation on growth rate of chicks and 43-day-old dressing percentage. The trials involved hatching eggs obtained from 27-wk-old hens and stored for 6 d for the Young-EXP group, from 28-wk-old hens and stored for 0 d for the Young-CON group, from 51-wk-old hens and stored for 6 d for the Old-EXP group, and from 52-wk-old hens and stored for 0 d for the Old-CON group, The hens were two commercial broiler breeder flocks of the same strain (Cobb) but of different egg producing stages(early and middle stages of egg production). The chicks were grown on floor pens for 6 wks, The differences of setting egg weights between Old-CON and Old-EXP groups were 1 g, but those between Young-CON and Young-EXP groups were 2.9 g(P<0,05). The loss of egg weight during 18 d incubation did not greatly differ among four groups, but the loss of egg weight during 21 d incubation was significantly (P<0.05) more in the middle stage of egg production groups than in the early stage of egg production groups. The mean birth weights of the middle stage of egg production groups were significantly(P<0,05) heavier by 8,7 g than those of the early stage of egg production groups; however, the differences of 6-wk-old body weight were not significant between egg producing stages. The differences of body weights in both egg producing stages were not significantly influenced by egg storage period in overall wks of ages. Egg storage and hen age did not greatly influence to the 43 d dressing percentages, either, The correlations of the setting egg weight with 18 d egg weight during incubation, growth rate of chicks, or 43 d dressing percentage were not significant.

• Journal of fish pathology
• /
• v.11 no.2
• /
• pp.113-117
• /
• 1998

The influence of temperature on the rate of egg production and embryonic development of Microcotyle sebastis was investigated to determine the precise time of a second treatment. The survival time of the adults of M. sebastis was inversely proportional to temperature. The number of laid eggs per each replicate during the first 24 h was $39.3{\pm}4.0$ at $10^{\circ}C$, $62.7{\pm}14.2$ at $15^{\circ}C$, $101.0{\pm}5.6$ at $20^{\circ}C$ and $89.0{\pm}11.0$ at $25^{\circ}C$. The time required for egg hatching of M. sebastis was $31.30{\pm}4.88$, $17.52{\pm}3.24$, $11.59{\pm}3.02$ and $10.76{\pm}3.10$ days at 10, 15, 20 and $25^{\circ}C$, respectively. The regression models of the time required for the beginning and 50% point of hatching according to the different temperatures were as follows; Beginning of hatch: D=58.2000-$4.2067{\times}Temp+0.0867{\times}(Temp)^2$ ($P\leq0.01$), 50% of hatch: D=91.3833-$7.5767{\times}Temp+0.1767{\times}(Temp)^2$ ($P\leq0.01$).