• 제목/요약/키워드: effect of heat treatment

검색결과 2,448건 처리시간 0.035초

Inhibition of Clostridium perfringens using Bacteriophages and Bacteriocin Producing Strains

  • Heo, Sunhak;Kim, Min Gon;Kwon, Mirae;Lee, Hee Soo;Kim, Geun-Bae
    • 한국축산식품학회지
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    • 제38권1호
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    • pp.88-98
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    • 2018
  • In this study, we isolated and characterized a bacteriocin-producing strain and two bacteriophages (P4, A3), showing antimicrobial effects against Clostridium perfringens, from chicken and swine feces by the spot-on-the lawn antagonism method. The selected strain was identified as Streptococcus hyointestinalis by 16S rRNA gene sequencing. The bacteriocin from the isolated strain exhibited strong inhibitory activity against four strains of C. perfringens and all the tested strains of Listeria monocytogenes, and the bacteriocin were highly heat- and pH-stable even at pH 2, pH 10 and $121^{\circ}C$ for 15 min. We also evaluated the combined effects of the isolated bacteriocin and phages. Combining the phage treatments and bacteriocin resulted in a synergetic effect compared with the phage or the bacteriocin alone. In addition, during the probiotic test, the bacteriocin-producing S. hyointestinalis B19 strain reduced the population of C. perfringens significantly. Treatment with S. hyointestinalis B19 and a cocktail of lytic bacteriophages eradicated the C. perfringens KCTC $3269^T$, completely. Consequently, the isolated bacteriocin and bacteriophages represent candidates for effective biocontrol of C. perfringens, and bacteriocin-producing S. hyointestinalis B19 is a potential probiotic candidate for use in domestic animals.

침탄처리한 SCM420H의 미끄럼 마모 특성에 미치는 침탄 조건의 영향 (Effects of Carburizing Process on Sliding wear Behavior of Carburized SCM420H Steel)

  • 이한영;이규헌
    • Tribology and Lubricants
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    • 제36권1호
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    • pp.18-26
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    • 2020
  • The effects of the carburizing process on the sliding wear behavior of SCM420H steel have been investigated. In particular, the effects of grain boundary corrosion observed in the surface layer after gas carburizing and the effects of hardness of the carburized cases after heat-treatment on the sliding wear properties were examined. Pin specimens carburized by two methods (gas carburizing and vacuum carburizing) were tempered at two temperatures of 180℃ and 400℃ after oil-quenching, respectively. Sliding wear tests were carried out against heattreated SKH51 steel at several sliding speeds using a pin-on-disc type test machine. As results, it can be found that there is no difference in the wear behavior between the pins carburized using two methods. This implies that the grain boundary corrosion that formed in the surface layer after gas carburizing has no effect on the sliding wear behavior of carburized SCM420H steels. Additionally, there is no significant difference in the wear behavior between carburized pins tempered at 400℃ and at 180℃ after oil-quenching, regardless of the carburizing method. This is because carburized pins tempered at 400℃ have a troostite structure, which exhibits higher tribochemical reactivity even though its hardness is lower than that of martensite structure. In this respect, it can be considered that good wear resistance of carburized cases is maintained at least until the effective case depth.

잔류응력과 계면접합강도를 고려한 금속복합재료의 열탄소성 변형 해석 (Thermal Elasto-Plastic Deformation Analysis of Metal Matrix Composites Considering Residual Stress and Interface Bonding Strength)

  • 강충길;서영호
    • 한국정밀공학회지
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    • 제16권1호통권94호
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    • pp.227-237
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    • 1999
  • As the interface bonding phenomenon between the matrix and the reinforcements has a large effect on the mechanical properties of MMCs, a sugestion of the strength analysis technique considering the residual stress and the interface bonding phenomenon is very important for the design of pans and the estimation of fatigue behavior. In this paper the three dimensional finite element anaysis is performed during the elasto-plastic deformation of the particulate reinforced metal matrix composites. It was analyzed with the volume fractions in view of microscale. Bonding strength. interface separation and matrix void growth between the matrix and the reinforcements will be predicted on deformation under tensile loading. An interface seperation is estimated by the fracture criterion which is a critical value of generalized plastic work per unit volume. The shape of the reinforcement is assumed to be a perfect sphere. And the type of the reinforcement distribution is assumed as FCC array. The thermal residual stress in MMCs is induced by the heat treatment. It is included at the simulation as an initial residual stress. The element birth and death method of the ANSYS program is used for the estimation of the interface bonding strength, void generation and propagation. It is assumed that the fracture in the matrix region begin to occur under the external loading when the plastic work per unit volume is equal to the critical value. The fracture strain will be defined. The experimental data of the extruded $SiC_p$>/606l Al composites are compared with the theoretical results.

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Th2 skewed condition 조절을 통한 대장점막 내 염증개선효과에 대한 두시 하태독법과 오메가3 비교 연구 (A Comparative Study of Douchi Hataedock versus Omega-3 on the Effect of Anti-inflammation in the Colonic Muscle through Th2 Skewed Condition Control)

  • 안상현;김기봉
    • 대한한방소아과학회지
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    • 제31권3호
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    • pp.37-45
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    • 2017
  • Objectives Hataedock is an orally-administered herbal extract that used to treat toxic heat and help excrete meconium accumulates in the fetus from pregnancy. This study compared the effects of Hataedock using the extract of Douchi and omega-3 to control inflammation in the colonic mucosa. Methods NC/Nga mice were divided into three groups; Control group (no treatment), FGT group (given the extract of Douchi), and O3T group (given omega-3). The 3-week-old mouse from each group were given the extract of Douchi and omega-3. After 2 weeks, the colonic mucosa tissues of the mice were observed via Immunohistochemical staining for IL-4, IL-13, CD40, $Fc{\varepsilon}RI$, $p-I{\kappa}B$, EGF in the intestinal mucosa. Results In both FGT and O3T group, IL-4, IL-13, STAT-6 levels and CD40-positive reactions were decreased. However, EGF-positive responses were increased. FGT group showed 35% decrease in IL-4, 45% decrease in IL-13, 48% decrease in CD40, 50% decrease in $Fc{\varepsilon}RI$, 54% decrease in $p-I{\kappa}B$, 52% increase in EGF compared to the control group. O3T group also showed 14% decrease in IL-4, 23% decrease in IL-13, 22% decrease in CD40, 29% decrease in $Fc{\varepsilon}RI$, 41% decrease in $p-I{\kappa}B$, 39% increase in EGF compared to the control group. Conclusions Both early administration of Douchi extract and omega-3 were effective in regulating Th2, relieving inflammation and developing colonic mucosal tissues. Hataedock with Douchi extract may be more effective in modulating immune system in colonic mucosa than omega-3.

Studies on Development of Porosity in Carbon from Different Types of Bio-wastes

  • Manocha, Satish M.;Chauhan, Vanraj B.;Manocha, L.M.
    • Carbon letters
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    • 제3권1호
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    • pp.1-5
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    • 2002
  • The regional bio-wastes available in abundance in India were converted into porous carbon by heat treatment at different temperatures from $650-950^{\circ}C$. The wood retain shapes after pyrolysis though shrinkage occured both in axial and radial directions. The shrinkage in radial direction was found to be more than in axial direction in all woods. The density of woods and chars from these at a given temperature has been found to follow linear relationship. Chars were steam activated at temperature $700-800^{\circ}C$ for different times between 45-240 min. Both the temperature and time of activation with steam has a profound effect on surface area. Chars from softwoods like bagasse and castor oil plant were activated at lower temperature, i.e. $700-750^{\circ}C$ whereas hard wood chars have to be activated at higher temperature around $800^{\circ}C$. The morphology of wood as well as of chars has been studied by SEM. The comparison of the two showed that the nature of porosity in chars depends on precursor morphology, nature and physical state of wood and presence of inorganic compounds in the wood. Hard wood results in cross inter connected pores while softwood leads to fibriller structure. The present studies show that activated carbon with reasonably good surface area (${\sim}1000m^2/gm$) can be prepared from soft wood bio-wastes like bagasse and castor oil plant, while surface area ${\sim}1370m^2/gm$ was achieved from hard wood bio waste of pine wood.

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액화천연가스 저장용 폐라이트계 Ni 첨가강의 물성 및 개발 동향 (State-of-the-Art of the Ni-bearing Ferritic Steels for LNG Storage Facilities)

  • 한승전;김형식;홍성호;김성준
    • 한국가스학회지
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    • 제2권3호
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    • pp.78-87
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    • 1998
  • 액화천연가스 저장용 소재로 널리 사용되고 있는 Ni 함유 페라이트 강은 $2-3\%,\;5.5\%,\;9\%$ 그리고 $13\%$ Ni 강으로 크게 분류되고 그 중에서도 경제성이 있으며 $-196^{\circ}C$까지의 온도에서 파괴인성과 용접성이 매우 우수한 $9\%$ Ni강이 가장 많이 사용되고 있다. 저온에서의 우수한 파괴인성은 Ni 첨가에 의한 잔류 오스테나이트 및 페라이트의 안정화 그리고 열처리효과에 기인한다. 최근 액화천연가스 저장탱크의 대형화에 따라 보다 두껍고 인성이 큰 소재가 요구되며, 따라서 이에 부응하는 저온용 Ni 함유 페라이트 강의 개발동향을 소개하였다.

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광량 및 TiO2 나노튜브 길이별 광활성 연구: Cr(VI)환원 및 수소제조 (Effect of TiO2 Nanotube Length on Photocatalytic Activity with Different Light Intensities: Cr(VI) Reduction and Hydrogen Production)

  • 주현규;심은정;이재민;윤재경
    • 한국수소및신에너지학회논문집
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    • 제22권4호
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    • pp.432-442
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    • 2011
  • Anodized tubular $TiO_2$ electrodes (ATTEs) with three noticeably different lengths are prepared to determine their optimum length for the photo-driven activity in the reaction of Cr(VI) reduction and hydrogen evolution. The ATTEs with ethylene glycol have longer $TiO_2$ tubes (7-15.6 ${\mu}m$) than those with hydrfluoric acid (0.6-0.8 ${\mu}m$). These samples, which differ only in the length of the tubes, with a wall thickness of ca. 20 nm, consist mainly of an anatase crystalline phase after heat treatment at $650^{\circ}C$, since the anatase crystallites at the tube walls do not undergo transformation into rutile phase, due to the constraints imposed by the wall thickness. Among them, the medium size (ca. 8 ${\mu}m$) tubes provide the optimum conditions, irrespective of the light intensity, which is explained in terms of the correlation between the amount of photons and the adsorbed electron acceptors and their location. Photocatalytic Cr(VI) reduction leads to ca. 60% reduction of Cr(VI) even under 1 sun irradiation with the medium-sized anodized $TiO_2$ tubes, but only ca. 20% with the short- and long-sized tubes. For hydrogen evolution, tubes longer than 8 ${\mu}m$ do not exhibit better performance with any light intensity.

Chemical Manganese Dioxide (CMD) 합성에서의 Seed의 열처리 효과 및 그 CMD로부터 제조되는 LiMn2O4의 전지특성 (Heat Treatment Effect of Seed on Synthesis of Chemical Manganese Dioxide (CMD) and Electrochemical Properties of LiMn2O4 obtained from the CMD)

  • 김성욱;조해란;노광철;박선민
    • Korean Chemical Engineering Research
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    • 제51권4호
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    • pp.460-464
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    • 2013
  • 본 연구에서는 $LiMn_2O_4$ 제조에서 Mn 원료로 사용되는 CMD를 seed 첨가법을 사용하여 제조하고자 하였으며, Seed의 열처리 온도가 CMD 합성에 미치는 영향을 고찰하고, 그로부터 제조되는 $LiMn_2O_4$의 전기화학적 특성을 평가하고자 하였다. 제조한 시료의 물성평가는 X-선 회절 분석법(XRD), 주사전자현미경(SEM)을 통하여 실시하였다. 그 결과, $MnCO_3$$300^{\circ}C$ 이상의 온도에서 열처리하여 seed로 사용할 경우 ${\gamma}-MnO_2$ 상의 CMD가 얻어졌으며, 그 CMD를 LMO 제조에 사용할 경우 전기화학적 특성이 비교적 우수한 LMO가 얻어졌다.

Effects of Swimming Exercise and Joint Mobilization on HSP 70 Levels in Osteoarthritic Rats

  • Kim, Se-Hum;Nam, Ki-Won;Seo, Dong-Yel
    • The Journal of Korean Physical Therapy
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    • 제26권6호
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    • pp.418-424
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    • 2014
  • Purpose: This study was performed to investigate the effect of joint mobilization on pain relief and cartilage repair in an induced osteoarthritis rat model by analyzing the expression of heat shock protein 70 in articular cartilage. Methods: MIA was injected into SD rats to induce osteoarthritis. These rats were divided into 4 groups: control group (n=30), no further treatment after the MIA injection ; experimental group I(n=30), performed swimming exercise after the MIA injection experimental group II (n=30), underwent joint mobilization after the MIA injection and experimental group III (n=30), performed swimming exercise and underwent joint mobilization after the MIA injection. For the histologic and pathophysiologic evaluation, safranin-O staining and for the immunohistochemical evaluation, the expression of HSP 70 in articular cartilage was analyzed 1, 7, 14, and 21 days after the MIA injection. Results: The inflammatory response and loss of tissue declined in experimental groups I and II over time, whereas the greatest decreases were noted in experimental group III. In the articular cartilage, low expression of HSP 70 was observed in every group on day 1, whereas HSP 70 expression was elevated on days 7 and 14 in experimental groups II and III. After 21 days, experimental group II displayed the strongest positive reaction, whereas HSP 70 was higher in experimental group III at this time point compared to that after 14 days. Conclusion: Our results showed that swimming exercise and joint mobilization had positive effects on pain relief and histologic and functional recovery in an induced osteoarthritis rat model.

심전도용 전극으로의 적용을 위한 폴리피롤 코팅 PVA 나노웹 전기전도성 텍스타일의 제조 (Production of Polypyrrole Coated PVA Nanoweb Electroconductive Textiles for Application to ECG Electrode)

  • 김재현;양혁주;조길수
    • 한국의류산업학회지
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    • 제21권3호
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    • pp.363-369
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    • 2019
  • This study developed electroconductive textiles by coating polypyrrole to PET nonwoven-based Polyvinyl Alcohol (PVA) nanoweb made by electrospinning and applying the developed electrotextiles as ECG Electrodes. To find the optimum coating conditions for high electrical conductivity, the ratios of 2.6-Naphthalenedisulfonic acid with Disodium Salt (NDS) vs Ammonium Persulfate (APS) as an oxidant and a doping agent in the solution were changed from 3:7 to 7:3; the immersion time of the specimen in the solution was 1 hour. PVA nanowebs coated with polypyrrole under various conditions were filmed with FE-SEM. FT-IR analysis was also performed to examine the presence of polypyrrole nanoparticles in the PVA nanoweb. The electrical resistance of the treated specimens were measured with a Multimeter. Consequently, the PVA Nano Web was undamaged even after heat treatment that allowed for coating. Uniform polypyrrole nanoparticles then formed on the surface of the PVA nanoweb after coating. The measured electrical resistance was shown to be at least $12K{\Omega}/{\Box }$ from a maximum of $3,456K{\Omega}/{\Box }$. The proper amount of NDS content had a positive effect on the conductivity improvement of electroconductive textiles; in addition, the highest electrical conductivity was achieved with a ratio of 3:7 between NDS and APS.