• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

• Journal of Intelligence and Information Systems
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• v.29 no.4
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• pp.15-30
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• 2023

Recently, various services using artificial intelligence(AI) are emerging in the media field as well However, most of the video editing, which involves finding an editing point and attaching the video, is carried out in a passive manner, requiring a lot of time and human resources. Therefore, this study proposes a methodology that can detect the edit points of video according to whether person in video are spoken by using Video Swin Transformer. First, facial keypoints are detected through face alignment. To this end, the proposed structure first detects facial keypoints through face alignment. Through this process, the temporal and spatial changes of the face are reflected from the input video data. And, through the Video Swin Transformer-based model proposed in this study, the behavior of the person in the video is classified. Specifically, after combining the feature map generated through Video Swin Transformer from video data and the facial keypoints detected through Face Alignment, utterance is classified through convolution layers. In conclusion, the performance of the image editing point detection model using facial keypoints proposed in this paper improved from 87.46% to 89.17% compared to the model without facial keypoints.

• Proceedings of the Korean Association for Survey Research Conference
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• 2005.12a
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• pp.159-167
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• 2005

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2019.05a
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• pp.558-560
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• 2019

Recently, there are steadily required more highly broadcasting service based on the broadcasting extension and improved quality. This paper proposed the method of the implemented above mentioned. The proposed high speed data link techniques method is more improved processing speed and more efficiently editing advantage compare with earlier methods.

• Molecules and Cells
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• v.40 no.11
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• pp.823-827
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• 2017

Genome editing using programmable nucleases such as CRISPR/Cas9 or Cpf1 has emerged as powerful tools for gene knock-out or knock-in in various organisms. While most genetic diseases are caused by point mutations, these genome-editing approaches are inefficient in inducing single-nucleotide substitutions. Recently, Cas9-linked cytidine deaminases, named base editors (BEs), have been shown to convert cytidine to uridine efficiently, leading to targeted single-base pair substitutions in human cells and organisms. Here, we first report on the generation of Xenopus laevis mutants with targeted single-base pair substitutions using this RNA-guided programmable deaminase. Injection of base editor 3 (BE3) ribonucleoprotein targeting the tyrosinase (tyr) gene in early embryos can induce site-specific base conversions with the rates of up to 20.5%, resulting in oculocutaneous albinism phenotypes without off-target mutations. We further test this base-editing system by targeting the tp53 gene with the result that the expected single-base pair substitutions are observed at the target site. Collectively, these data establish that the programmable deaminases are efficient tools for creating targeted point mutations for human disease modeling in Xenopus.

• Proceedings of the Korea Contents Association Conference
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• 2008.05a
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• pp.147-151
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• 2008

Since the first HDV(High Definition Video) camcoder, HDR-FX1 with 1080i resolution, had been launched at 2004, the image capture and record with HD resolution have been possible by the various types of camcoder and recorder which can support the HDV format. At a present day, a non-linear editing system, which is composed of only one computer, can capture, edit and export the image with HDV format. The HDV format has the same class of resolution with HD and the same data transfer bit rate of DV(Digital Video). Additionally, the image with HDV format can be recorded in 6mm DV cassette tape. It is possible because the HDV format uses the concept of Long GOP(Gorup of pictures) method for its compression. In this paper, the characteristic feature of HDV format, the essential factors in editing with HDV format and the concept of Long GOP method for its compression have been studied for the way of effective HDV editing.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 1997.06a
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• pp.75-81
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• 1997

A new DTPP (Desk-Top Program Production) system has been developed that enables multiple program producers (directors) working at different locations to collaborate over a computer network and prepare a single program for broadcasting. In this system, information is shared among users by exchanging data edited on non-linear editing terminals in program post-production work over a network in real time. In short, the new DTPP system provides a collaborative work space for producing TV programs. The system does not make use of a special server for collaborative work but rather multiple interconnected editing terminals having the same functions. In this configuration, data at a terminal which has just been edited by some operation is forwarded to all other connected terminals for updating. This form of information sharing, however, requires that some sort of data synchronizing method be established since multiple terminals are operating on the same data simultaneously. We therefore adopt a method whereby the system synchronizes the clocks on each terminal at the time of connection and sends an operation time stamp together with edited data. This enables most recently modified data to be identified and all information on all terminals to be updated appropriately. This paper provides an overview of this new collaborative DTPP system and describes the techniques for exchanging edited data and synchronizing data.

• Journal of the Korean Association of Geographic Information Studies
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• v.8 no.3
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• pp.129-141
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• 2005

It is difficult to edit large-scale digital maps because of problems of cost and process and it is carried out by aerial photogrammetry in renewal periods. Five years of update period cannot provide exact data required in the fast-moving age. This study is to analyze applicability and impact for editing digital map of road system using the GPS-Van. Results are compared with accuracy of the data acquisition with GPS-Van positioning. INS data are affected by the barrier to receive GPS data. But high accuracy were achieved by thorough plans according to work order. By using GPS Van and fieldwork at the same time for editing and renewal of digital map, it is expected that this method can be used to reduce costs in the economic and temporal aspects, and provide rapid and accurate digital map of road system.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.3
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• pp.308-317
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• 2011

A MBES (multibeam echo sounder) survey around Yokji Island, Korea, was conducted to find an effective method for removing error data. Two post-processing software programs, PDS2000 (RESON) and HIPS (CARIS), were used to remove the error data using an interactive editing method and the CUBE algorithm filter. The post-processing with the PDS2000 and HIPS programs, using the interactive editing method, took 120 and 168 hours, respectively, and there was little difference in the seafloor images. The processing time of the PDS2000 and HIPS programs using the CUBE algorithm filter was 36 and 60 hours, respectively. Nevertheless, there was little difference in the seafloor images because of differences in the factor parameters in each of the post-processing programs. Therefore, post-processing using CUBE filtering can save time in data processing and provide consistent results, excluding the subjective decisions of the operator. This method is more effective than other methods for rejecting erroneous multibeam echo sounder data.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.3
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• pp.28-37
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• 1996

To reduce an authors burden in hypermedia system that allows non-sequential information the process of creating links must be easy. However, most of the conventional hypermedia systems possess two difficulties. First, the author must go through several troublesome process to create a single link. Secondly, it is not easy to create an anchor in text or other multimedia data. Therefore, in order to support effective construction of hypermedia system the editing environment must provide an easy method to create links. In this paper, to resolve the weaknesses of conventional hypermedia system as mentioned above, an editing tool is developed and implemented to easily create the links of multimedia data. There are three methods in creating links and a user can select a convenient method in given circumstances. And for teh efficient production of nodes composed of multimedia information, we provide an authoring environment to integrate and process those informations.