• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.194-194
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.200-200
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• BMB Reports
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• 제57권1호
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• pp.19-29
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• 2024

Mitochondrial DNA (mtDNA), a multicopy genome found in mitochondria, is crucial for oxidative phosphorylation. Mutations in mtDNA can lead to severe mitochondrial dysfunction in tissues and organs with high energy demand. MtDNA mutations are closely associated with mitochondrial and age-related disease. To better understand the functional role of mtDNA and work toward developing therapeutics, it is essential to advance technology that is capable of manipulating the mitochondrial genome. This review discusses ongoing efforts in mitochondrial genome editing with mtDNA nucleases and base editors, including the tools, delivery strategies, and applications. Future advances in mitochondrial genome editing to address challenges regarding their efficiency and specificity can achieve the promise of therapeutic genome editing.

• 공학논문집
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• 제3권1호
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• pp.21-27
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• 1998

본 논문에서는 SGML DTD(Document Type Definition)의 문서 구조정보를 이용하여 SGML 실례문서를 편집하기 위한 시스템을 설계 및 구현하였다. 이를 위해 문서의 논리구조 표현을 위한 구조 창을 이용하여 SGML 문서를 편집할 수 있어 SGML에 대해 모르는 사용자도 편집오류 없이 문서를 생성할 수 있고 엘리먼트(element)와 속성(attribute), 엔티티(entity)를 지원하는 도구를 이용하여 엘리먼트 등을 손쉽게 수정 가능하고, 생성된 문서를 SGML 파서(parser)를 이용하여 검증할 수 있도록 시스템을 설계하였다. 또한 본 시스템은KS 5601코드를 사용하여 한글과 영문 텍스트를 모두 지원한다. 본 논문에서 설계한 SGML 문서 편집 시스템은 윈도우 사용자 인터페이스를 위해 윈도우95 시스템 환경 하에서 구현하였다.

• Molecules and Cells
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• 제41권10호
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• pp.917-922
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• 2018

The CRISPR-Cas system is a well-established RNA-guided DNA editing technique widely used to modify genomic DNA sequences. I used the CRISPR-Cas9 system to change the second and third nucleotides of the triplet $T{\underline{CT}}$ of human TNSFSF9 in HepG2 cells to $T{\underline{AG}}$ to create an amber stop codon. The $T{\underline{CT}}$ triplet is the codon for Ser at the $172^{nd}$ position of TNSFSF9. The two substituted nucleotides, AG, were confirmed by DNA sequencing of the PCR product followed by PCR amplification of the genomic TNFSF9 gene. Interestingly, sequencing of the cDNA of transcripts of the edited TNFSF9 gene revealed that the $T{\underline{AG}}$ had been re-edited to the wild type triplet $T{\underline{CT}}$, and 1 or 2 bases just before the triplet had been deleted. These observations indicate that CRISPR-Cas9-mediated editing of bases in target genomic DNA can be followed by spontaneous re-editing (correcting) of the bases during transcription.

• 생명과학회지
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• 제8권2호
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• pp.203-207
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• 1998

본 연구는 옥수수에서 분리한 미토콘드리아에서 NADH-dehydrogenase 유전자 (subunit 4)의 cDNA를 RT-PCR의 방법을 사용하여 조제 한 ㅜ 염기서열 수행한 경과 특이한 점을 감지 할 수 있었다. 일반적인 RNA cditing은 C에서 U로 또는 U에서 C로 치환되는 현장으로 옥수수의 NAD4유전자에서도 이러한 editing 형상이 일어나는 것을 발견하였다. 또는 T가 G로 그리고 G 가 A로 변화되는 특이한 부분들이 생성되는 것을 관찰하였다. 이러한 RNA ediring은 주로 exon 1과 exon 4 에 많이 일어나며, 염기 치환되는 부분들은 에서늬 NAD4유전자의 RNA edting site들과 일피하지 않은 점으로 미루어 보아 RNA editing 현상은 무작의로 생성된다고 본다.된다고 본다.

• Bulletin of the Korean Chemical Society
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• 제28권2호
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• pp.207-210
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• 2007

To identify structural or functional common subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through the sequence alignments and structural overlapping of the CP1 domains, three conserved regions were identified near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed the existence of three common structural subunits in all of the five editing RS structures. Based on the established experimental results and our modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through the most class Ia editing RSs.

• Molecules and Cells
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• 제44권12호
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• pp.911-919
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• 2021

The virus-induced genome editing (VIGE) system aims to induce targeted mutations in seeds without requiring any tissue culture. Here, we show that tobacco rattle virus (TRV) harboring guide RNA (gRNA) edits germ cells in a wild tobacco, Nicotiana attenuata, that expresses Streptococcus pyogenes Cas9 (SpCas9). We first generated N. attenuata transgenic plants expressing SpCas9 under the control of 35S promoter and infected rosette leaves with TRV carrying gRNA. Gene-edited seeds were not found in the progeny of the infected N. attenuata. Next, the N. attenuata ribosomal protein S5 A (RPS5A) promoter fused to SpCas9 was employed to induce the heritable gene editing with TRV. The RPS5A promoter-driven SpCas9 successfully produced monoallelic mutations at three target genes in N. attenuata seeds with TRV-delivered guide RNA. These monoallelic mutations were found in 2%-6% seeds among M₁ progenies. This editing method provides an alternative way to increase the heritable editing efficacy of VIGE.

• BMB Reports
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• 제57권1호
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• pp.12-18
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• 2024

Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the off-target problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized.

• 한국조사연구학회지:조사연구
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• 제6권1호
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• pp.83-98
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• 2005

대규모 통계조사에서 수집된 데이터에는 오류나 결측값의 문제가 발생하기 마련이다. 조사, 데이터 입력, 데이터 처리 등의 과정에서 여러 가지 요인에 의해 이런 문제가 생길 수 있는데 이런 데이터를 방치한 채 통계를 생산할 경우 편향이나 다양한 분석에서의 불일치의 문제가 발생하게 되어 통계의 품질과 신뢰성을 떨어뜨릴 수 있으므로 수집된 데이터의 오류나 결측값을 찾아 수정하는 데이터편집은 매우 중요한 작업이다. 해외에서는 데이터편집의 문제를 공론화하여 다루고 있는 데 반해 우리나라에서 데이터편집에 관한 논의는 거의 없는 편이다. 본 연구의 목적은 주택가 격동향조사를 위한 데이터편집의 사례를 소개함으로 데이터편집에 대한 논의의 폭을 넓히는 데 있다. 조사목적에 맞도록 편집규칙을 정하는 과정 및 관련 자료들을 소개하고, 온라인조사라는 조사방식에 맞는 입력 데이터편집방법을 마련하여 실시하는 예들을 소개하며, 마지막으로 출력 데이터편집에 의해 입력 편집에서 걸러지지 않은 오류나 문제들을 제거하는 방법도 소개한다.