• Journal of Plant Biotechnology
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• 제38권2호
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• pp.125-129
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• 2011

GM작물의 상용화를 위하여서는 반드시 환경위해성평가를 거쳐야 한다. 불행하게도 초기 event가 개발되고 기본적인 검토가 완료된 GM작물에 대하여 환경위해성평가를 수행하기 위하여서는 약 7백만에서 1,500만불의 경비가 소요되며 기간 또한 수년이 필요하다. 따라서 저자는 사용화를 위한 GM작물의 개발을 위한 프로젝트에 관여하는 개발자는 실험실 또는 온실단계의 아주 초기단계에서부터 환경위해성평가기준을 충분히 숙지하여야 한다고 제안한다. 기존의 많은 리뷰논몬에서 이미 지적된 것 과 같이 GM작물의 상용화과제에서 가장 큰 걸림돌(죽음의 계곡)은 초기 사상 (event)의 선발과정이며, 대부분이 이 단계에서 충분한 자료를 제시할 수 없어서 포기한다는 사실을 간과하여서는 안 될 것이다. 특히 지극히 기본적인 분자생물학 특성에 관한 자료들로서 예를 들어 도입유전자의 copy 수, 숙주의 게놈내 도입위치, 도입유전자의 주변 염기서열, 유전자 재배열과 도입유전자로 인한 숙주 내 기존유전자의 knock out 현상의 여부 등에 관한 충분한 자료를 제시하지 못하는 경우가 많으며 이는 GM 작물의 개발 초기단계에서부터 철저하게 검증이 이루어져야 하는 항목들이다. 국내의 경우, 농촌진흥청은 2011년부터 2020년까지 10년간 "차세대바이오그린 21 사업"을 발족하여 추진 중에 있으며 특히 국내용뿐만 아니라 중국의 사막화 지역 등 불량환경에 재배가 가능한 GM작물 등 해외로 수출을 위한 global GM작물의 개발을 위한 전략을 수립하여 추진 중에 있다. 따라서 저자는 환경위해성평가기준에 부합되는 초기 event의 선발 과정에 역점을 두어 사전에 분자생물학적 특성에 관한 RA기준을 고려하여 자체평가시스템을 도입하도록 제안 한다.

• Clinics in Orthopedic Surgery
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• 제10권4호
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• pp.448-454
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• 2018

Background: Traumatic spinal cord injury (SCI) is a tragic event that has a major impact on individuals and society as well as the healthcare system. The purpose of this study was to investigate the strength of association between surgical treatment timing and neurological improvement. Methods: Fifty-six patients with neurological impairment due to traumatic SCI were included in this study. From January 2013 to June 2017, all their medical records were reviewed. Initially, to identify the factors affecting the recovery of neurological deficit after an acute SCI, we performed univariate logistic regression analyses for various variables. Then, we performed a multivariate logistic regression analysis for variables that showed a p-value of < 0.2 in the univariate analyses. The Hosmer-Lemeshow test was used to determine the goodness of fit for the multivariate logistic regression model. Results: In the univariate analysis on the strength of associations between various factors and neurological improvement, the following factors had a p-value of < 0.2: surgical timing (early, < 8 hours; late, 8-24 hours; p = 0.033), completeness of SCI (complete/incomplete; p = 0.033), and smoking (p = 0.095). In the multivariate analysis, only two variables were significant: surgical timing (odds ratio [OR], 0.128; p = 0.004) and completeness of SCI (OR, 9.611; p = 0.009). Conclusions: Early surgical decompression within 8 hours after traumatic SCI appeared to improve neurological recovery. Furthermore, incomplete SCI was more closely related to favorable neurological improvement than complete SCI. Therefore, we recommend early decompression as an effective treatment for traumatic SCI.

• 의정연구
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• 제26권2호
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• pp.113-140
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• 2020

이 연구는 공간적 자기상관성(spatial autocorrelation)이라는 개념을 사용해 한국 선거를 처음으로 분석했다는 점에서 의미가 있다. 공간적 자기상관성이란, 공간상의 한 위치에서 발생하는 사건은 그 주변 지역에서 발생하는 사건과는 상관관계가 높다는 것을 의미한다. 제21대 총선 서울지역의 투표율을 관내 사전투표율과 본투표율로 나누고 투표율의 공간적 패턴이 나타나는지 살펴보았다. 기존의 연구가 선거구 단위를 토대로 분석한 것이 대부분이고 개인자료를 사용한 것이라면 이 연구에서는 좀 더 하위 단위인 읍면동 단위를 기준으로 분석했고 공간자료와 집합자료를 사용해 분석하였다. 본투표율의 모란 I (Moran's I) 지수는 0.261로 꽤 높은 공간적 자기상관성을 보인 반면 관내사전투표율의 지수는 0.095로 낮아 통계적 유의성이 있음에도 불구하고 공간적 자기상관성이 거의 없는 것으로 나타났다. 공간적 자기상관성이 강하게 나타난 본투표율을 OLS 회귀모델과 공간통계모델로 비교해 분석해보았다. 일반 회귀모델에서 결정계수인 R²가 0.585261에서, 공간오차모델에서는 0.656631로 상승하여 약 7퍼센트포인트의 설명력 증가를 볼 수 있어 공간통계모델이 설명력이 높다는 사실을 알 수 있었다. 가장 흥미로운 결과는 관내사전투표율과 본투표율의 관계인데, 관내사전투표율이 높은 동은 본투표율이 낮게 나오고, 관내사전투표율이 2% 정도 올라가면 본투표율은 약 1% 정도 떨어지는 것으로 조사되었다. 이 연구에서는 관내사전투표율과 본투표율에 영향을 미치는 변수는 매우 다르고, 관내사전투표율의 상승폭이 본투표율 하락폭과 다르다는 점에서 투표편의제공에 따른 분산효과로만 볼 수 없다는 것을 알 수 있어 기존 연구와 차별성을 가진다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5675-5680
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• 2013

Background: Preexisting type 2 diabetes mellitus (T2DM) affects the prognosis and mortality of patients with some cancers. Insulin like growth factor (IGF) and insulin receptor (IR) signaling axes play important roles in both cancer and diabetes development. We aimed to explore the expression characteristics of proteins in IGF/IR axis in non-small cell lung cancer (NSCLC) cases with preexisting T2DM. Methods: Fifty-five NSCLC patients with preexisting T2DM were retrospectively included and matched by 55 NSCLC without diabetes at a 1:1 ratio. The expression of proteins in IGF/IR axis was detected by immunohistochemical staining. Clinicopathological data were collected to analyze their relationship with the protein expression. Results: Both IGF 1 receptor (IGF-1R) and insulin receptor substrate 2 (IRS-2) showed higher expression in the NSCLC with T2DM group, compared with those without T2DM. The high expression of IGF-1R and IRS-2 were found to be negatively associated with lymph node metastases and T staging in the T2DM group, respectively, and IRS-2 expression was also found more in the subgroup whose T2DM duration was more than 4 years. No difference was detected in the expression of IRS-1, IGF-1, IGF-2, IGFBP3, IR and mTOR between groups with or without T2DM. Conclusion: Our study found higher expression of IGF-1R and IRS-2 proteins in NSCLC patients with preexisting T2DM, and that there was an association with early stage NSCLC, which suggested that IGF signaling may play an important early event in development of NSCLC associated with diabetes.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.231-243
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• 1998

In the present study, we provide evidence that ginsenoside $Rh_2$ (G-$Rh_2$) as well as staurosporine induces apoptosis of human hepatoma SK-HEP-1 cells by caspase 3-mediated processing of $p21^{WAFI/CIPI}$ in the early stage of apoptosls. Immunoblottings showed that G-$Rh_2$ as well as statrosporine induced the processing of caspase-3 to an active form, pl7. In stable Bcl-2 transfectants however, G-$Rh_2$ induced DNA fragmentation, while staurosporine did not. In the early stage of apoptosis, $p21^{WAFI/CIPI}$ was detected to undergo proteolytic processing specifically conducted by caspase-3. $p21^{WAFI/CIPI}$ translated in vitro was cleaved into a p14 fragment, when incubated with cell extracts obtained from either G-$Rh_2$- or staurosporine-treated cells. Cleavage was equally inhibited in both cases by adding Ac-DEVD-cho, a specific caspase-3 inhibitor, but not by Ac-YVkD-cho, a specific caspase-l inhibitor. Similarly, $p21^{WAFI/CIPI}$ was efficiently leaved by recombinant caspase-3 overexpressed in E. coli. Moreover, the endogenous $p21^{WAFI/CIPI}$ of untreated-cell extracts was also cleaved by recombinant caspase-3. Mutation analysis allowed identification of two caspase-3 cleavage sites, $DHVD^{112}$/L and $SMTD^{149}$/F, which are located within, or near the interaction domains for cyclins, Cdks, and PCNA. Taken together, these results show that G-$Rh_2$ as well as staurosporine increases caspase-3 activity, which in turn directly cleaves $p21^{WAFI/CIPI}$ resulting in elevation of Cdk kinase activity in the early stages of apoptosis. We propose that proteolytic cleavage of $p21^{WAFI/CIPI}$ is a functionally relevant event that allows unleashing the cyclin/Cdk activity from the inhibitor seen in the earlier stage of apoptosis, the event of which may be associated with the triggering mechanism for the execution of apoptosis.

• 한국건설순환자원학회논문집
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• 제4권3호
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• pp.90-97
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• 2009

본 연구에서는 고로슬래그 미분말(이하 BS)사용 모르타르의 초기강도가 저하하는 문제점을 해결하고자 BS 20%치환 시멘트 모르타르에 자극제로 석고(이하 CS)와 고분말도의 미분시멘트(이하 FC)를 치환 사용한 경우의 기초적 물성을 검토한 것으로, 그 결과를 요약하면 다음과 같다. 먼저, 굳지않은 모르타르의 특성으로 유동성은 BS 치환율이 증가할수록 감소하였고, BS 20%에 CS를 치환한 경우는 치환율에 관계없이 큰 영향을 미치지 않은 것으로 나타났으며, FC를 치환한 경우는 치환율이 증가할수록 감소하는 것으로 나타났다. 압축강도는 BS 치환율이 증가할수록 초기재령에서 강도가 저하하는 것으로 나타났고, 재령 28일에서는 잠재수경성 반응에 기인하여 약간 증가하는 것으로 나타났다. BS 20 % 치환 모르타르의 초기 압축 및 휨 강도향상에 대하여 CS를 치환한 경우는 치환율이 증가함에 따라 초기재령에서 미소하게 증가하는 것으로 나타났고, FC를 치환한 경우는 치환율이 증가할수록 초기 및 28일 재령에서 강도가 크게 증가하는 것으로 나타났다. 종합적으로 BS 20% 치환 모르타르의 초기 강도 저하 문제의 해결로서 CS의 치환은 효과가 적고, FC 25%를 치환하여 사용하게 되면 OPC만을 사용한 Plain과 유사한 것으로 나타나 양호한 품질의 향상을 기대할 수 있을 것으로 분석된다.

• 건축역사연구
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• 제29권3호
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• pp.67-78
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• 2020

This study examines the transitional situation that can be seen from the palace operation in early Joseon Dynasty. The first decades of Joseon after the nation was founded differ from the Joseon Dynasty as a whole, but rather similar with the Goryeo Dynasty. By examining "The History of Goryeo(高麗史)," it showed that palace operation during the Goryeo Dynasy was marked by the fact that a separate palace was built and resided in despite the existence of the Bongweol(本闕, main palace) as the central palace. The separation of the parent's generation and the children's generation was shown through the establishment of one's own palace. Such trait of Goryeo affected palace construction directly after the founding of Joseon. This can be shown from the characteristic of palace operation by generation. The construction of the Changdeokgung Palace(昌德宮) of King Taejong, as his own palace, is an example. Afterwards, the palace system of Joseon was established, and the space of the king and the crown prince was merged through ritual reform during the reign of King Sejong. However, the space for the parents of the king were considered separate, and this influence continued to the reign of King Seongjong. The construction of Changgyeonggung Palace(昌慶宮) during the reign of King Seongjong is an event where the king proclaimed the separation with his mother and grandmother.

• Interdisciplinary Bio Central
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• 제2권2호
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• pp.5.1-5.9
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• 2010

Flavobacterium columnare (F. columnare), the dermotropic Gram negative yellow pigmented bacteria was isolated from different sites of skin ulcerations in the Nile tilapia (Oreochromis niloticus) and Nile catfish (Clarias gariepinus) collected from an earthen pond located at an aquaculture station in Sharkiya Province, Lower Egypt during an acute episode of mass kills during the early summer of 2009. An acute infection with F. columnare was behind the emergent event of mass mortalities among both populations. Many of the Nile tilapias exhibited typical signs of hole - in- the head like lesions from which F. columnare together with the myxosporean spore, Myxobolus tilapiae (M. tilapiae) were retrieved. Most of the cohabitating infected Nile catfishes exhibited severe form of saddle back like ulcer. The identities of the retrieved isolates were confirmed using morphological, biochemical and molecular tools. The research lead us to conclude that the two diverse etiological agents (F. columnare and M. tilapiae) under the triggering effect of the abrupt change in the water quality measures (abrupt rise in the water temperature, ammonia, pH, sharp decrease in dissolved oxygen) have synergized together to induce the above mentioned pathology with the consequent reemergence of fish mass mortalities.

• 대한불안의학회지
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• 제10권2호
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• pp.103-107
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• 2014

Objective : The objective of this study was to examine the association between childhood adversity and parenting attitude and neuroticism in adulthood. Methods : Forty nine women were recruited from community and completed Early Trauma Inventory-Short Form (ETI-SF). We compared scores on the Maternal Behavior Research Instrument (MBRI), Neuroticism of NEO Personality Inventory (NEO-PI), and Beck Depression Inventory (BDI) between women with and without childhood adversity. Results : Compared to women without childhood adversity, women with childhood adversity showed more rejecting parenting attitude and higher Depression and Self-Consciousness scores on Neuroticism domain of NEO-PI. Total ETI-SF scores and emotional abuse scores were positively correlated with rejecting attitude scores on the MBRI and BDI scores. Physical abuse scores were positively correlated with rejecting attitude scores. There were positive correlations between rejecting attitude scores on the MBRI, Neuroticism scores on the NEO-PI, and BDI scores. Conclusion : Our results suggest that childhood adversity may have a negative impact on parenting attitude and emotional state in adulthood. For girls who experienced traumatic event, early intervention is needed to prevent the development of neurotic temperament and rejecting parenting in adulthood.

• 복식
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• 제54권2호
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• pp.79-94
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• 2004

The main purpose of this study is to discribe the transfiguration process and the symbols of theatrical costume of Sandai masque(산대놀이) during the Choson Dynasty. Sandai masque as a popular play is a very important form in the history of the Korean theatre. Furthermore in order to analyze the mutual relationship and difference between in the early and later Chosen Dynasty, Narye has is stressed deeply a national amusement event. In the early years of Choson Dynasty, non-official culture such as outside of Seoul and Seoul were subject to the official culture of the Court. In the late years, actors become more and more libral, separating themselves from the systemical restriction. The main reason for this is the growth of capitals in private sectors as well as the growth in demand for entertainment among private citizens. On these backgrounds, the transfiguration process of Sandai masque have changed the treatrical costume. The treatrical costume carries symbolic meaning to the obserber. The value of symbol changes as the time passes, overtime thereby altering its meaning as well. The caracters and plots change inevitably over years but the basic theme remains. The symbols of theatrical costume of Sandai masque are mainly focused on three subjects: first, the entrance of a dragon and tiger originated in the exorcism for good harvest; second, color symbolism expressed in the confrontation between red and black; and third, associate characteristics of dress forms related to regoinal classification of the mask dance.