• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.167-179
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• 2023

The rifampicin-resistant strain E9-302 of Edwardsiella ictaluri strain 669 (WT) was generated by continuous passage on BHI agar plates containing increasing concentrations of rifampicin. E9-302 was attenuated significantly by 119 times to zebrafish Danio rerio compared to WT in terms of the 50% lethal dose (LD₅₀). Zebrafish vaccinated with E9-302 via intraperitoneal (IP) injection at a dose of 1 × 10³ CFU/fish had relative percentage survival (RPS) rates of 85.7% when challenged with wild-type E. ictaluri via IP 14 days post-vaccination (dpv). After 14 days of primary vaccination with E9-302 via immersion (IM) at a dose of 4 × 10⁷ CFU/ml, a booster IM vaccination with E9-302 at a dose of 2 × 10⁷ CFU/ml exhibited 65.2% RPS against challenge with wild-type E. ictaluri via IP 7 days later. These results indicated that the rifampicin-resistant attenuated strain E9-302 had potential as a live vaccine against E. ictaluri infection. A previously unreported amino acid site change at position 142 of the RNA polymerase (RNAP) β subunit encoded by the gene rpoB associated with rifampicin resistance was identified. Analysis of the whole-genome sequencing results revealed multiple missense mutations in the virulence-related genes esrB and sspH2 in E9-302 compared with WT, and a 189 bp mismatch in one gene, whose coding product was highly homologous to glycosyltransferase family 39 protein. This study preliminarily explored the molecular mechanism underlying the virulence attenuation of rifampicin-resistant strain E9-302 and provided a new target for the subsequent study of the pathogenic mechanism of E. ictaluri.

• The Journal of the Acoustical Society of Korea
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• 제20권3E호
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• pp.3-9
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• 2001

This study aims to describe a prosodic pattern on the Korean subjective particles with respect to their discourse function. 4 kinds of Korean subjective particles were mainly investigated with reference to sentential location, grammatical relations that precede or follow the word including subjective particles, and prosodic phrasing. F0 and energy were gradually diminished as the particles moved down to the sentential final position. 'Ga'particle, which has been potentially regarded as having a grammatical focusing function, looks like to show relatively higher F0 in sentential medial in discourse. At sentential medial position, when the words including 'ga, eun, and neun'particles were preceded by adverbials, the acoustic variables of particles tended to be diminished by some ratio in comparison with the mean value. The duration of particles might vary with respect to style variation and especially that it tended to diminish from 150 basic, 50 separate, and finally 50 discourse successively. And there's some specific phenomenon that prosodic phrasing itself was relatively easily taken place after 'eun' and 'neun' particles. Finally, I tried to catch the prosodic characteristics (which would be established as acoustic features) of inter-word position at which specific subjective particles were intervened. These acoustic features can be made up of the duration and F0 fluctuation activated in the successive 3 syllables in which word (or prosodic) boundary was located.

• Journal of Electrical Engineering and Technology
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• 제12권1호
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• pp.329-334
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• 2017

This paper discusses the near field characteristics of a dipole source located near conducting metallic walls from an electromagnetic compatibility (EMC) point of view. An integral equation for a dipole source near a metallic wall is derived and solved by applying Galerkin's method of moments (MoM). The results show that in the regions outside the dipole source, total electric near fields decrease gradually in magnitude with an increasing field point from the dipole source. But in the regions inside the dipole source, total electric near fields decrease rapidly with a dipole position of $h{\leq}0.3{\lambda}$. For a dipole position of $h{\geq}0.7{\lambda}$, the peaks and nulls of the total near electric field occur periodically in the regions inside the dipole source, and the fluctuation period is almost $0.5{\lambda}$. The worst position for a receptor location is along the z-axis, and a range of a half-magnitude of the maximum near electric field in the principal H-plane is about two times broader than that of the principal E-plane. Experimental measurements are also presented to validate the theory.

• Journal of Biosystems Engineering
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• 제34권5호
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• pp.331-341
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• 2009

A double-end rod hydraulic cylinder is widely used with a steering valve for the steering control system in large tractors. For the development of automatic steering controller, the feasibility of using a proportional control valve replacing the conventional manual steering valve to control the position of hydraulic steering cylinder was investigated in terms of the max. overshoot, the steady-state error and the rise time. A simulation model for the electrohydraulic steering system with load using AMESim package was developed to be valid so that the proper control algorithm could be chosen through the computer simulation. It could be concluded that the P-control algorithm was sufficient to control the electrohydraulic steering system, where the control frequency should be no greater than 20 Hz at the P-gain of 5. In particular, the performance of the developed steering controller was satisfactory even at the conditions of varying flow rates and loads.

• The Journal of the Acoustical Society of Korea
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• 제24권1E호
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• pp.28-33
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• 2005

This study was designed to suggest an assessment tool for analyzing the characteristics of Korean phonetic contrast intelligibility among dysarthric individuals. The intelligibility deficit factors of phonetic contrast in Korean dysarthric patients were analyzed through stepwise regression analysis. The 19 acoustic-phonetic contrasts proposed by Kent et al. (1999) have been claimed to be useful for clinical assessment and research on dysarthria. However, the test cannot be directly applied to Korean patients due to linguistic differences between English and Korean. Thus, it is necessary to devise a Korean word intelligibility test that reflects the distinct characteristics of the Korean language. To identify the speech error characteristics of a Korean dysarthric group, a Korean word list was audio-recorded by 3 spastic, 4 flaccid, and 5 mixed type of dysarthric patients. The word list consisted of monosyllabic consonant-vowel-consonant (CVC) real word pairs. Stimulus words included 41 phonemic contrast pairs and six triplets. The results showed that the percentage of errors in final position contrast was higher than in any other position. Unlike the results of previous studies, the initial-position contrasts were crucial in predicting the overall intelligibility among Korean patients.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.372-375
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• 2006

The MSC is a high resolution multi-spectral camera system which is mounted on the KOMPSAT-II satellite. The electro-optic camera system has a refocusing mechanism which can be used in-orbit by ground commands. By adjusting locations of some elements in optics, the system can be focused precisely. The focus mechanism in MSC is implemented with stepper motor and potentiometer. By reading the value of the potentiometer, rough position of the motor can be understood. The exact location of the motor can not be acquired because the information from the potentiometer can not be so accurate. However, before and after certain events of the satellite, like a satellite launch, the direction of the movement or order of the magnitude of the movement can be understood. In this paper, the trend analysis of the focus motor position during the ground test phase is introduced. This result can be used as basic information for the focus calibration after launch. By studying the long term trend, deviation from the best focal point can be understood. The positions of the focus motors after launch are also compared.

• 대한치과기공학회지
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• 제30권1호
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• pp.9-15
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• 2008

An investigation was carried out to examine the pores of casting bodies depending on the position of wax pattern in the ring for precious alloy by optical micrographs. From the result of optical micrographs, many pores were found in the Group C that wax pattern is placed in the center of the ring. However, any pores were scarcely found in the Group A, B, D and E that wax patterns are placed out of the center of thermal zone. In comparison with the pores of casting bodies of Group C, many pores were found at the cusp area rather than at the proximal surface. From these examination, it was concluded that wax pattern should be placed out of the center of thermal zone to prevent casting boies from occurring pores.

• Journal of Positioning, Navigation, and Timing
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• 제6권1호
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• pp.27-33
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• 2017

GPS provides the positioning solution in most areas of the world. However, the position error largely occurs in the urban area due to signal attenuation, signal blockage, and multipath. Although many studies have been carried out to solve this problem, a definite solution has not yet been proposed. Therefore, research is being conducted to solve the vehicle localization problem in the urban environment by converging sensors such as cameras and Light Detection and Ranging (LIDAR). In this paper, the precise vehicle localization using 3D LIDAR (Velodyne HDL-32E) is performed in the urban area. As there are many tall buildings in the urban area and the outer walls of urban buildings consist of planes generally perpendicular to the earth's surface, the outer wall of the building meets at a vertical corner and this vertical corner can be accurately extracted using 3D LIDAR. In this paper, we describe the vertical corner extraction method using 3D LIDAR and perform the precise localization by combining the extracted corner position and GPS/DR information. The driving test was carried out in an about 4.5 km-long section near Teheran-ro, Gangnam. The lateral and longitudinal RMS position errors were 0.146 m and 0.286 m, respectively and showed very accurate localization performance.

• 동력기계공학회지
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• 제15권6호
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• pp.22-26
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• 2011

A numerical simulation on the flow field was carried out on the cylindrical shell with baffles. The steady incompressible 3-D Navier-Stokes solution is obtained with the actual operational condition and geometry of the heat exchanger. The effect of the location of inlet and outlet on the cylindrical shell with baffle is investigated by varying flow rate. The angle between the location of In/Outlet and baffle cutting part is $0^{\circ}$, $30^{\circ}$, $60^{\circ}$, $90^{\circ}$, $120^{\circ}$, $150^{\circ}$ and $180^{\circ}$. The present results show that the pressure drop is dependent on Reynolds number in the inlet area and position of inlet and outlet; i.e., the pressure drop increases with increasing Reynolds number and the pressure drop decreases with increasing angle between baffle cutting part and position of inlet and outlet.

• 한국염색가공학회지
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• 제17권5호
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• pp.53-60
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• 2005

Fusing press m/c is used for heating and pressing the specimens that are fed into between the two moving belts. Therefore the belt movement, belt temperature, and the pressure between belts must be kept constant. Especially, the belts should move in a limited operation range. When the belts run far out of the operation point, the machine has to be stopped, which results in a product defect because the fusing conditions, e.g., temperature and pressure, change during the transient process time period. It is important to avoid the belt stopping by maintaining the belt movement in a limited range. This study reports about the movement of the endless fusing belt in a long-span roller fusing m/c. The belt position changes as the 1st-order system does; if the roller axes are slanting each other with a certain angle, the belt running around the two rolls shows a dynamic behavior with the time that deviates fastly at the beginning from the initial condion and slows down. Then it reaches at a final position. The skewer the axes, the greater the position change. The inital change rate of the belt becomes large as the skewness of the axes between the two rollers increases.