• Proceeding of EDISON Challenge
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• 2016.03a
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• pp.90-95
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• 2016

Heat Shock 70kDa Protein 1-Like(HSPA1L)는 Heat-shock protein70(HSP70) family에 속하는 chaperone protein으로 polypeptide folding, assembly, protein degradation 등 다양한 biological processes에 관여하고 있다. HSPA1L은 human methyltransferase-like protein 21A(METTL21A)에 의해 lysine residue에 methylation이 일어나게 되는데, 암세포에서 일반적인 HSPA1L은 주로 세포질에서 발견되는 반면 methylated HSPA1L의 경우 주로 핵에서 발견이 됨으로써 HSPA1L methylation이 암 세포 성장에 중요할 역할을 할 것이라 추측되며 anti-cancer drug target으로 주목 받고 있다. 하지만 현재 HSPA1L의 구조가 부분적으로만 밝혀져 있어 HSPA1L와 METTL21A가 어떤 residue들이 interaction 하여 binding을 하는지에 대해서 아직 밝혀 지지 않았다. 이로 인해 anti-cancer drug target으로서의 연구에 제한이 있다. 이번 연구에서는 homology modeling(Galaxy-TBM, Galaxy-refine)을 통해 HSPA1L 전체 구조를 밝혀 낸 후, HSPA1L 와 METTL21A를 protein-protein docking을 통해 binding pose 예측을 하였다. 이러한 binding pose를 protein interaction analysis하여 HSPA1L과 METTL21A binding에 관여하는 중요 residue들을 밝혀 냈다. 이러한 structural information은 methylated HSPA1L와 암 세포 성장간의 연관성, 더 나아가 anti-cancer drug 개발로 까지도 이어 질 수 있을 것이라 생각한다.

• Information and Communications Magazine
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• v.24 no.3
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• pp.87-96
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• 2007

HSPA는 HSDPA 및 HSUPA를 통합하여 일컫는 3GPP 기술로 하향 링크에서는 HSDPA를 상향 링크에서는 HSUPA를 사용하여 기존 DCH만을 사용하는 3GPP Release 99 시스템 대비 더 효율적인 고속 멀티미디어 서비스를 제공하는 기술이다. HSDPA는 3GPP Release 5 기술로서 2002년 3월 첫 표준이 승인되었으며 단말의 무선 환경에 따라 변조 및 코딩기법을 변화시키는 AMC, 물리 계층을 통해 빠른 재전송을 지원하는 HARQ,단축된 2ms TTI 그리고 Node-B 기반의 고속 스케줄링을 통해 무선망 성능을 획기적으로 향상시켜 하향 링크에서 최대 14.4Mbps를 제공한다. HSUPA는 3GPP Release 6 기술로서 2004년 6월 첫 표준이 승인되었으며 HSDPA에 도입한 기술들 중에서 AMC를 제외한 모든 기술을 적용하여 상향 링크에서 최대 5.76Mbps를 제공한다. HSPA Evolution(eHSPA 또는 HSPA+)은 HSPA의 성능 개선을 통해 3GPP Release 8 기술인 LTE로의 자연스러운 진화를 보장하기 위한 3GPP Release 7 기술로 2006년 3월 TSG RAN #31 회의에서 승인되었다. 본 고에서는 최근 표준화가 활발히 진행되고 있는 HSPA Evolution에서 최근까지 승인된 각 계층별 요소 기술에 대해 소개하고자 한다.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.2
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• pp.24-30
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• 2009

In this paper, the FPGA modem platform complying with 3GPP Release 7 eHSPA specifications and its regularized verification flow are proposed. The FFGA platform consists of modem board supporting physical layer requirements, MCU and DSP core embedded control board to drive the modem board, and peripheral boards for RF interfacing and various equipment interfaces. On the other hand, the proposed verification flow has been regularized into three categories according to the correlation degrees of hardware-software inter-operation, such as simple function test, scenario test call processing and system-level performance test. When it comes to real implementations, the emulation verification strategy for low power mobile SoC is also introduced.

• The Journal of the Korea institute of electronic communication sciences
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• v.9 no.4
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• pp.491-498
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• 2014

As internet services are being provided through a wireless access, the importance of quality of experience (QoE) is stressed that is defined as the quality that indicates user's actual feeling when a service is provided. Unlike quality of service (QoS) that can be expressed as a numerical value, it is difficult to represent QoE in an objective way. If an internet service is serviced over a wireless channel, its QoE can be affected by a number of factors such as fading, mobility and so on. This paper, therefore, attempts to specify the relationship between QoE and QoS by conducting practical measurements for the voice service through 3G high speed packet access (HSPA) access network. Analysing the measured results, it has been shown that received signal strength indicator (RSSI) has a great influence on mean opinion score (MOS) through transmission delay.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1155-1164
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• 2019

Lichens contain diverse bioactive secondary metabolites with various chemical and biological properties, which have been widely studied. However, details of the inhibitory mechanisms of their secondary metabolites against influenza A virus (IAV) have not been documented. Here, we investigated the antiviral effect of lichen extracts, obtained from South Korea, against IAV in MDCK cells. Of the lichens tested, Nipponoparmelia laevior (LC24) exhibited the most potent inhibitory effect against IAV infection. LC24 extract significantly increased cell viability, and reduced apoptosis in IAV-infected cells. The LC24 extract also markedly reduced (~ 3.2 log-fold) IAV mRNA expression after 48 h of infection. To understand the antiviral mechanism of LC24 against IAV, proteomic (UPLC-$HDMS^E$) analysis was performed to compare proteome modulation in IAV-infected (V) vs. mock (M) and LC24+IAV (LCV) vs. V cells. Based on Ingenuity Pathway Analysis (IPA), LC24 inhibited IAV infection by modulating several antiviral-related genes and proteins (HSPA4, HSPA5, HSPA8, ANXA1, ANXA2, $HIF-1{\alpha}$, AKT1, MX1, HNRNPH1, HNRNPDL, PDIA3, and VCP) via different signaling pathways, including $HIF-1{\alpha}$ signaling, unfolded protein response, and interferon signaling. These molecules were identified as the specific biomarkers for controlling IAV in vitro and further confirmation of their potential against IAV in vivo is required. Our findings provide a platform for further studies on the application of lichen extracts against IAV.

• Biomedical Science Letters
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• v.15 no.1
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• pp.97-99
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• 2009

Geldanamycin is a benzoquinone ansamycin antibiotic that binds to cytosol HSP90 (Heat Shock Protein 90) and changes its biological function. HSP90 is involved in the intracellular important roles for the regulation of the cell cycle, cell growth, cell survival, apoptosis, angiogenesis and oncogenesis. To identify genes expressed during geldanamycin treatment against neurons of rats (PC12 cells), DNA microarray method was used. We have isolated 2 gene groups (up-or down-regulated genes) which are geldanamycin differentially expressed in neurons. Granzyme B is the gene most significantly increased among 204 up-regulated genes (more than 2 fold over-expression) and Chemokine (C-C motif) ligand 20 is the gene most dramatically decreased among 491 down-regulated genes (more than 2 fold down-expression). The gene increased expression of Cxc110, Cyp11a1, Gadd45a, Gja1, Gpx2, Ifua4, Inpp5e, Sox4, and Stip1 are involved stress-response gene, and Cryab, Dnaja1, Hspa1a, Hspa8, Hspca, Hspcb, Hspd1, Hspd1, and Hsph1 are strongly associated with protein folding. Cell cycle associated genes (Bc13, Brca2, Ccnf, Cdk2, Ddit3, Dusp6, E2f1, Illa, and Junb) and inflammatory response associated genes (Cc12, Cc120, Cxc12, Il23a, Nos2, Nppb, Tgfb1, Tlr2, and Tnt) are down-regulated more than 2 times by geldanamycin treatment. We found that geldanamycin is related to expression of many genes associated with stress response, protein folding, cell cycle, and inflammation by DNA microarray analysis. Further experimental molecular studies will be needed to figure out the exact biological function of various genes described above and the physiological change of neuronal cells by geldanamycin. The resulting data will give the one of the good clues for understanding of geldanamycin under molecular level in the neurons.

• The Journal of Society for e-Business Studies
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• v.16 no.3
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• pp.145-158
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• 2011

Recently, the rapidly increased use of smartphone devices provides both opportunity and problems at the same time to the telecom companies in domestic and world wide. It provides a great opportunity to overcome the market stagnation of the existing voice- centric communications, and also problems of building a highly efficient new wireless access networks to accommodate huge volume of data traffic among smart devices. Facing with the age of keen competition, telecom companies have two kinds of strategies for network transition; a strategy of building uniform LTE -based 4G networks as fast as possible, and a hybrid network enhancement strategy compounding 3G and 4G networks using HSP A+, WiFi, cloud computing, etc. It is difficult to predict which strategy will have more successful outcome in the near future. This paper studies the trends and issues of network enhancement policies and transition strategies to the 4G network era, and tries to draw appropriate and efficient ways for network enhancement and operation.