• 한국정보전자통신기술학회논문지
• /
• 제12권6호
• /
• pp.681-689
• /
• 2019

웹 사용 패턴 발견은 웹 로그 데이터를 사용하는 고급 수단이며 웹 로그 데이터 마이닝에 데이터 마이닝 기술을 적용한 특정 응용이다. 교육 분야에서 데이터 마이닝 (DM)은 데이터 마이닝 기술을 교육 데이터 (대학의 웹 로그, e-러닝, 적응형 하이퍼미디어 및 지능형 튜터링시스템 등)에 적용한다. 따라서 교육 연구 문제를 해결하기 위해 이러한 유형의 데이터를 분석하는 것이 목표이다. 본 논문에서는 대학의 웹 로그 데이터가 데이터 마이닝의 연구 대상으로 사용되어 진다. 데이터베이스 OLAP 기술을 사용하여 웹 로그 데이터가 데이터 마이닝에 사용될 수 있는 데이터 형식으로 사전 처리되고 그 처리 결과가 MSSQL에 저장된다. 동시에 처리 된 웹 로그 레코드를 기반으로 기본 데이터 통계 및 분석이 완료된다. 또한 웹 사용 패턴 마이닝의 Apriori Algorithm 및 구현 프로세스를 소개하고 Python 개발 환경에서 Apriori Algorithm 프로그램을 개발했다. 그런 다음 Apriori Algorithm의 성능을 보이고 웹 사용자 액세스 패턴의 마이닝을 실현했다. 이 연구 결과는 교육 시스템 개발에 패턴을 적용하는데 중요한 이론적 의미를 갖는다. 다음 연구로는 분산 컴퓨팅 환경에서 Apriori Algorithm의 성능 향상을 연구하는 것이다.

• 대한지리학회지
• /
• 제46권6호
• /
• pp.767-780
• /
• 2011

이 논문은 '인정(recognition)'을 통해 인간과 공간의 관계에 대해 살펴보았다. 인정은 인간의 근원적 욕구이다. 인간은 누구든지 어디서나 인정받으려 한다. 자신의 정체성을 강화하고, 자신의 존재이유를 확인받기 위해 인정투쟁을 벌인다. 헤겔이 제시한 '인정'은 주체-객체의 인간관에 근거한 상호인정 과정에 근거한다. 반면에, 서로 주체성을 토대로 하는'인정'은 나와 너의 관계를 토대로'우리'를 강조하는 인간관에 근거한다. '인정'은 인간과 공간의 관계에 대한 새로운 인식을 가능하게 한다. 지금까지 지리학에서 다루었던 보이는 공간에 대한 지리적 인식을 넘어, 보이지 않는 공간에 대한 지리학의 역할을 채근한다. 보이지 않는 공간을 보이게 하는 과정을 통해 지리학의 본질을 회복하는데 기여한다. 목소리가 들리지 않는 공간과 이야기가 쓰여지지 않는 공간에 담겨있는 지리적 의미를 발견하도록 돕는다. 이를 통해 우리 사회의 모순과 부조리한 모습을 고발하고, 본질적으로 문제를 해결할 수 있는 방법을 제시한다. 이같은 논의의 궁극적 목적은 지리학이 당면한 위기를 극복하고, 인문학으로서 세상과 소통하기 위한 것이다.

• Animal Bioscience
• /
• 제36권1호
• /
• pp.10-18
• /
• 2023

Objective: In this study, we aimed to position the Hungarian Merino among other Merinoderived sheep breeds, explore the characteristics of our sampled animals' genetic similarity network within the breed, and highlight single nucleotide polymorphisms (SNPs) associated with daily weight-gain. Methods: Hungarian Merino (n = 138) was genotyped on Ovine SNP50 Bead Chip (Illumina, San Diego, CA, USA) and positioned among 30 Merino and Merino-derived breeds (n = 555). Population characteristics were obtained via PLINK, SVS, Admixture, and Treemix software, within-breed network was analysed with python networkx 2.3 library. Daily weight gain of Hungarian Merino was standardised to 60 days and was collected from the database of the Association of Hungarian Sheep and Goat Breeders. For the identification of loci associated with daily weight gain, a multi-locus mixed-model was used. Results: Supporting the breed's written history, the closest breeds to Hungarian Merino were Estremadura and Rambouillet (pairwise F_ST values are 0.035 and 0.036, respectively). Among Hungarian Merino, a highly centralised connectedness has been revealed by network analysis of pairwise values of identity-by-state, where the animal in the central node had a betweenness centrality value equal to 0.936. Probing of daily weight gain against the SNP data of Hungarian Merinos revealed five associated loci. Two of them, OAR8_17854216.1 and s42441.1 on chromosome 8 and 9 (-log₁₀P>22, false discovery rate<5.5e-20) and one locus on chromosome 20, s28948.1 (-log₁₀P = 13.46, false discovery rate = 4.1e-11), were close to the markers reported in other breeds concerning daily weight gain, six-month weight, and post-weaning gain. Conclusion: The position of Hungarian Merino among other Merino breeds has been determined. We have described the similarity network of the individuals to be applied in breeding practices and highlighted several markers useful for elevating the daily weight gain of Hungarian Merino.

• Korean Journal of Radiology
• /
• 제22권9호
• /
• pp.1514-1524
• /
• 2021

Objective: To develop a radiomics risk score based on dynamic contrast-enhanced (DCE) MRI for prognosis prediction in patients with glioblastoma. Materials and Methods: One hundred and fifty patients (92 male [61.3%]; mean age ± standard deviation, 60.5 ± 13.5 years) with glioblastoma who underwent preoperative MRI were enrolled in the study. Six hundred and forty-two radiomic features were extracted from volume transfer constant (K^trans), fractional volume of vascular plasma space (V_p), and fractional volume of extravascular extracellular space (V_e) maps of DCE MRI, wherein the regions of interest were based on both T1-weighted contrast-enhancing areas and non-enhancing T2 hyperintense areas. Using feature selection algorithms, salient radiomic features were selected from the 642 features. Next, a radiomics risk score was developed using a weighted combination of the selected features in the discovery set (n = 105); the risk score was validated in the validation set (n = 45) by investigating the difference in prognosis between the "radiomics risk score" groups. Finally, multivariable Cox regression analysis for progression-free survival was performed using the radiomics risk score and clinical variables as covariates. Results: 16 radiomic features obtained from non-enhancing T2 hyperintense areas were selected among the 642 features identified. The radiomics risk score was used to stratify high- and low-risk groups in both the discovery and validation sets (both p < 0.001 by the log-rank test). The radiomics risk score and presence of isocitrate dehydrogenase (IDH) mutation showed independent associations with progression-free survival in opposite directions (hazard ratio, 3.56; p = 0.004 and hazard ratio, 0.34; p = 0.022, respectively). Conclusion: We developed and validated the "radiomics risk score" from the features of DCE MRI based on non-enhancing T2 hyperintense areas for risk stratification of patients with glioblastoma. It was associated with progression-free survival independently of IDH mutation status.

• Mass Spectrometry Letters
• /
• 제8권4호
• /
• pp.114-118
• /
• 2017

While saliva can be considered as good biological fluid for monitoring biomarkers due to many advantages including its communication with blood and the non-invasive nature during its sampling, its applications to that purpose is still limited. As a part of efforts to expand the applications of saliva to the protein biomarker research, we carried out global absolute quantitation of proteins in human whole saliva (WS) by bottom-up proteomics techniques mainly based on nLC-Q-IMS-TOF employing $MS^E$. From the analyses of a pooled WS sample collected from 22 healthy Korean volunteers, 93 proteins ranging from $5.89{\times}10^1ng/mL$ (immunoglobulin heavy chain) to $1.59{\times}10^4ng/mL$ (${\alpha}-amylase$ 1) were confirmed. For the validation of the present results, human serum albumin in the same sample was quantitated by ELISA and its result was compared with that from the nLC-Q-IMS-TOF study. As a result, there was no significant difference between two results from individual approaches ($1.18{\times}10^4{\pm}0.03{\times}10^4 ng/mL$ from nLC-Q-IMS-TOF experiments vs. $1.23{\times}10^4{\pm}0.07{\times}10^4ng/mL$ from ELISA experiments, n=3, p=0.309). To our knowledge, this is the first global absolute quantitation of proteins in human whole saliva and information from the present study can be widely used as the first level reference for the discovery of new protein biomarkers from human whole saliva as well as for quantitative applications of human whole saliva proteins.

• 동의생리병리학회지
• /
• 제21권5호
• /
• pp.1260-1270
• /
• 2007

The discovery of drugs on the treatment of mast cell-mediated allergic disease is a very important subject in human heath. The Socheongyoug-tang(SCYT) has been used for centruries as a traditional medicine in Korea and is known to have an anti-inflammatory effect. However, its specific mechanism of action is still unknown. In this report, we investigated the effect of hot water extract from SCYT on RBL-2H3 mast cell-mediated allergic reaction and studied its possible mechanism of action. SCYT inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. SCYT decreased the passive cutaneous anaphylaxis reaction activated by Anti-lgE antibody-HSA. SCYT dose-dependently reduced histamine release from mice peritoneal mast cells activated by Anti-lgE antibody-HSA. SCYT increased cAMP and decreased compound 48/80-induced intracellular $Ca^{2+}$ levels. Our findings provide evidence that SCYT inhibits mast-derived allergic reactions, and also demonstrate the involvement of cAMP and intracellular $Ca^{2+}$ in these effects.

• Journal of Microbiology and Biotechnology
• /
• 제30권7호
• /
• pp.1097-1103
• /
• 2020

Bacterial surface display systems have been developed for various applications in biotechnology and industry. Particularly, the discovery and design of anchoring motifs is highly important for the successful display of a target protein or peptide on the surface of bacteria. In this study, an efficient display system on Escherichia coli was developed using novel anchoring motifs designed from the E. coli mipA gene. Using the C-terminal fusion system of an industrial enzyme, Pseudomonas fluorescens lipase, six possible fusion sites, V¹⁴⁰, V¹⁷⁶, K¹⁷⁹, V²²⁶, V²³², and K²³⁴, which were truncated from the C-terminal end of the mipA gene (MV¹⁴⁰, MV¹⁷⁶, MV¹⁷⁹, MV²²⁶, MV²³², and MV²³⁴) were examined. The whole-cell lipase activities showed that MV¹⁴⁰ was the best among the six anchoring motifs. Furthermore, the lipase activity obtained using MV¹⁴⁰ as the anchoring motif was approximately 20-fold higher than that of the previous anchoring motifs FadL and OprF but slightly higher than that of YiaTR232. Western blotting and confocal microscopy further confirmed the localization of the fusion lipase displayed on the E. coli surface using the truncated MV¹⁴⁰. Additionally the MV¹⁴⁰ motif could be used for successfully displaying another industrial enzyme, α-amylase from Bacillus subtilis. These results showed that the fusion proteins using the MV¹⁴⁰ motif had notably high enzyme activities and did not exert any adverse effects on either cell growth or outer membrane integrity. Thus, this study shows that MipA can be used as a novel anchoring motif for more efficient bacterial surface display in the biotechnological and industrial fields.

• The Plant Pathology Journal
• /
• 제21권1호
• /
• pp.21-26
• /
• 2005

Homeotic proteins have pivotal roles during the development of both plant and animals. Many homeotic proteins exert control over cell fate in cells where their genes are not expressed, i.e., in a non-cell autonomous manner. Cell-to-cell communication, which delivers critical information for position-dependent specification of cell fate, is an essential biological process in multicellular organisms. In plants, there are two pathways for intercellular communication that have been identified: the ligand/receptor-mediated apoplastic pathway and the plasmodesmata-mediated symplasmic pathway. Regulatory proteins and RNAs traffic symplasmically via plasmodesmata and play a critical role in intercellular communication. Thus, the non-cell autonomous function of homeotic proteins can be explained by the recent discovery of cell-to-cell trafficking of proteins or RNAs. This article specifically focuses on understanding the intercellular movement of homeodomain proteins, a family of homeotic proteins.

• 한국전기전자재료학회논문지
• /
• 제33권2호
• /
• pp.147-150
• /
• 2020

Relatively pure YBCO was first synthesized by heating a mixture of metal carbonates at temperatures between 1,000 and 1,300 K, resulting in the reaction: 4BaCO₃+Y₂(CO₃)₃+6CuCO₃+(1/2_-x)O₂ → 2YBa₂Cu₃O_7-x+1/3CO₂. Modern syntheses of YBCO use the corresponding oxides and nitrates. The superconducting properties of YBa₂Cu₃O_7-x are sensitive to the value of x, i.e., its oxygen content. Only those materials with 0≤x≤0.65 are superconducting below Tc, and when x ~ 0.07, the material superconducts at the highest temperature, i.e., 95 K, or in the highest magnetic fields, i.e., 120 T and 250 T when B is perpendicular and parallel to the CuO₂ planes, respectively. In addition to being sensitive to the stoichiometry of oxygen, the properties of YBCO are influenced by the crystallization methods applied. YBCO is a crystalline material, and the best superconductive properties are obtained when crystal grain boundaries are aligned by careful control of annealing and quenching temperature rates. However, these alternative methods still require careful sintering to produce a quality product. New possibilities have arisen since the discovery of trifluoroacetic acid, a source of fluorine that prevents the formation of undesired barium carbonate (BaCO₃). This route lowers the temperature necessary to obtain the correct phase at around 700℃. This, together with the lack of dependence on vacuum, makes this method a very promising way to achieve a scalable YBCO bulk.

• 한국컴퓨터정보학회논문지
• /
• 제10권1호
• /
• pp.101-112
• /
• 2005

최근에 동적으로, 즉 요구가 있는 즉시 서비스들을 조합하는 동적 웹 서비스 조합은 가장 큰 관심사 중하나이다. 특히, 사용자가 현존하는 하나의 웹 서비스로는 요구사항들을 만족시켜줄 수 있는 기능이 없을 때 그러한 요구를 만족시키기 위해 몇 개의 서비스들을 동적으로 결합해야 하는 문제가 큰 이슈로 부각되고 있다. 본 논문에서는 비즈니스 분야에서 성공적으로 활용되고 있는 워크플로우 기법을 적용하여 동적웹 서비스 조합을 구현한다. 워크플로우의 웹 서비스 적용은 아직까지는 새로운 분야로써 다음과 같은 두가지 문제 해결을 요구한다. (1) 인터넷상에 흩어져 있는 수많은 웹 서비스들 중에서 어떻게 원하는 서비스를 효율적으로 탐색할 수 있는가, (2) 탐색된 다양한 이기종 웹 서비스들 간의 상호운용성 극대화 문제에 관한 해결이 필요하다. 본 연구에서는 (1)을 수행하기 위해 웹 서비스 매칭 알고리즘이 제안되고, (2)를 해결하기 위해 매칭 알고리즘에 온톨로지 개념이 적용된다. 그리고 어떻게 동적 뀁 서비스 조합이 구현되는지 설명하기 위해 하나의 프로토타입 시스템을 구축한다.