• Natural Product Sciences
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• 제16권3호
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• pp.185-191
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• 2010

Mast cell-mediated allergic disease is involved in many diseases such as anaphylaxis, asthma and atopic dermatitis. The discovery of drugs for the treatment of allergic disease is an important subject in human health. In the present study, the effect of water extract of Gleditsiae Spina (WGS) (Leguminosae), on compound 48/80-induced systemic allergic reaction, anti-DNP IgE antibody-induced local allergic reaction, and histamine release from human mast cell line (HMC-1) cells were studied. In addition, the effect of WGS on phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187 (A23187)-induced gene expression and secretion of pro-inflammatory cytokines were investigated using HMC-1 cells. WGS was anally administered to mice for high and fast absorption. WGS inhibited compound 48/80-induced systemic allergic reaction. WGS dose-dependently decreased the IgE-mediated passive cutaneous anaphylaxis. WGS reduced histamine release from HMC-1 cells. In addition, WGS decreased the gene expression and secretion of pro-inflammatory cytokines in PMA plus A23187-stimulated HMC-1 cells. These findings provide evidence that WGS could be a candidate as an antiallergic agent.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.323-326
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• 2011

While the presence of pinworm eggs in archaeological samples has been reported by many researchers in the New World, those have been detected very scarcely in the Old World, especially in East Asian countries. In fact, many parasite species were recovered from the archeological remains in Korea, eggs of Enterobius vermicularis had not been found. Recently, a female mummy buried in the 17th century was discovered in the Joseon tomb from Dangjin-gun, Chungcheongnam-do, Korea. After rehydration process for 12 days, investigations were carried on the luminal surface of the colon. From them, 3 eggs of E. vermicularis were recovered. They were elliptical, transparent with a thin egg shell, $50.3{\pm}5.2\;{\mu}m$ (length) and $28.2{\pm}3.9\;{\mu}m$ (width) in size. This is the first discovery of E. vermicularis eggs in East Asia.

• Molecules and Cells
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• 제27권4호
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• pp.391-396
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• 2009

Image-based, high-content screening assays demand solutions for image segmentation and cellular compartment encoding to track critical events - for example those reported by GFP fusions within mitosis, signalling pathways and protein translocations. To meet this need, a series of nuclear/cytoplasmic discriminating probes have been developed: DRAQ5$^{TM}$ and CyTRAK Orange$^{TM}$. These are spectrally compatible with GFP reporters offering new solutions in imaging and cytometry. At their most fundamental they provide a convenient fluorescent emission signature which is spectrally separated from the commonly used reporter proteins (e.g. eGFP, YFP, mRFP) and fluorescent tags such as Alexafluor 488, fluorescein and Cy2. Additionally, they do not excite in the UV and thus avoid the complications of compound UV-autofluorescence in drug discovery whilst limiting the impact of background sample autofluorescence. They provide a convenient means of stoichiometrically labelling cell nuclei in live cells without the aid of DMSO and can equally be used for fixed cells. Further developments have permitted the simultaneous and differential labelling of both nuclear and cytoplasmic compartments in live and fixed cells to clearly render the precise location of cell boundaries which may be beneficial for quantitative expression measurements, cell-cell interactions and most recently compound in vitro toxicology testing.

• 천문학회보
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• 제46권2호
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• pp.69.2-69.2
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• 2021

We present the discovery of rare active galactic nuclei (AGNs) in nearby (z<0.05) compact elliptical galaxies (cEs) located in isolated environments. Using spectroscopic data from the Sloan Digital Sky Survey (SDSS) Data Release 12, four AGNs were identified based on the optical emission-line diagnostic diagram. SDSS optical spectra of AGNs show the presence of distinct narrow-line emissions. Utilizing the black hole (BH) mass-stellar velocity dispersion scaling relation and the correlation between the narrow L([OIII])/L(Hβ) line ratio and the width of the broad Hα emission line, we estimated the BH masses of the cEs to be in the range of 7×10⁵-8×10⁷ solar mass. The observed surface brightness profiles of the cEs were fitted with a double Sérsic function using the Dark Energy Camera Legacy Survey r-band imaging data. Assuming the inner component as the bulge, the K-band bulge luminosity was also estimated from the corresponding Two Micron All Sky Survey images. We found that our cEs follow the observed BH mass-stellar velocity dispersion and BH mass-bulge luminosity scaling relations, albeit there was a large uncertainty in the derived BH mass of one cE. In view of the observational properties of BHs and those of the stellar populations of cEs, we discuss the proposition that cEs in isolated environments are bona fide low-mass early-type galaxies (i.e., a nature origin).

• 정보처리학회논문지D
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• 제12D권6호
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• pp.889-898
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• 2005

최근에 동적으로, 즉 요구가 있는 즉시 서비스들을 조합하는 동적 웹 서비스 조합은 가장 큰 관심사 중 하나이다. 지금까지 동적 웹 서비스조합에 관한 많은 연구들이 수행되어졌는데 주로 사람이 개입된 반자동 대화식 조합 기법들이 제안되었다. 또한 이들 연구에서 탐색 알고리즘 및 워크플로우 설계 기법들이 부분적으로는 기술되어 있으나. 웹 서비스 조합 자동화를 위한 전반적인 워크플로우 처리과정을 기술한 연구는 아직 없다. 본 논문은 웹 서비스 조합 자동화 기법에 초점을 맞추고 있다. 주된 아이디어는 시멘틱 웹을 실현하기 위한 웹 온톨로지와 비즈니스분야에서 성공적으로 활용되고 있는 워크플로우 기법들을 적용하여 웹 서비스 조합 자동화 시스템을 구현하는 것이다. 본 연구에서는 웹 서비스발견을 위해 매칭 알고리즘이 제안되고, 웹 서비스들 간 상호연결을 지원하기 위해 매칭 알고리즘에 온톨로지 개념이 적용된다. 그리고 웹 서비스 조합 자동화를 지원하기 위한 워크플로우 실행계획이 기술된다. 마지막으로 실 데이터 실험을 통해 제안 시스템의 우수성을 보인다.

• 산업공학
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• 제25권1호
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• pp.21-30
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• 2012

In the past, buyers and suppliers met each other to find common interests off-line in exhibitions and conferences, or through personal connection. These activities were time consuming and costly. With the advent of information era, these activities moved to online market places, where buyers search for suppliers with a set of keywords that are believed to be representative of their requirements. Its fundamental assumption is that all the potential candidates are registered in a certain database. However, recently buyers want to diversify suppliers due to needs of cost competitiveness or frequent new product development. To this end, instead of choosing suppliers from the supplier pool, discovering suppliers from all over the world should be emphasized. In order to enable buyers to describe their requirements and suppliers to capture their manufacturing capabilities via online market places, the semantic differences of terms between buyers and suppliers should be resolved. The paper summarizes various supplier discovery frameworks and prototype systems, which can be employed to expose domestic small-medium enterprises into global buyers in the near future.

• Nuclear Engineering and Technology
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• 제19권4호
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• pp.233-241
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• 1987

본 연구는 제한된 수의 핵연료의 경험적 파손자료로부터 핵연료 파손 확률을 현실적으로 예측하기 위해 결정론적 모델로부터의 파손화률 예측치와 실제 경험적 자료로부터의 파손 확률 예측치를 접합하는 방법을 시도하였다. 이 접합 방법에 의한 파손 화률 예측치는 결정론적 모델 또는 경험적 파손 자료로부터의 독립적인 예측치보다 신뢰도가 높다. 본 연구에서는 핵연료 성능 예측코드인 SPEAR의 방법론을 응용한 핵연료 파손 패턴의 체계적 발견법 (hierarchical pattern discovery)이 접합 모델에서의 결정론적 모델로부터의 예측치에 대한 가중치와 패턴 경계를 체계적으로 찾기 위해 고안되었다. 이 연구에서 개발된 접합 방법을 PROFIT모델과 경험적 파손자료를 이용하여 CANDU형 핵연료 재장전중 출력 상승에 의해 수반되는 핵연료파손 예측에 적응시켜 보았다.

• BMB Reports
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• 제38권5호
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• pp.624-631
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• 2005

Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the main diseases to mankind. It is urgent to discover novel drug targets for appropriate antimicrobial agents against this human pathogen. The shikimate pathway is onsidered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammalian cells. The Mycobacterium tuberculosis aroE-encoded shikimate dehydrogenase was cloned, expressed and purified. Sequence alignment analysis shows that shikimate dehydrogenase of Mycobacterium tuberculosis exhibit the pattern of G-X-(N/S)-V-(T/S)-X-PX-K, which is highly conserved within the shikimate dehydrogenase family. The recombinant shikimate dehydrogenase spectrum determined by CD spectroscopy showed that the percentages for $\alpha$-helix, $\beta$-sheet, $\beta$-turn, and random coil were 29.2%, 9.3%, 32.7%, and 28.8%, respectively. The enzymatic characterization demonstrates that it appears to be fully active at pH from 9.0 to 12, and temperature $63^{\circ}C$. The apparent Michaelis constant for shikimic acid and $NADP^+$ were calculated to be about $29.5\;{\mu}M$ and $63\;{\mu}M$. The recombinant shikimate dehydrogenase catalyzes the substrate in the presence of $NADP^+$ with an enzyme turnover number of $399\;s^{-1}$. Zymological studies suggest that the cloned shikimate dehydrogenase from M. tuberculosis has a pretty activity, and the work should help in the discovery of enzyme inhibitors and further of possible antimicrobial agents against Mycobacterium tuberculosis.

• 한국축산식품학회지
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• 제42권3호
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• pp.389-397
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• 2022

Carcass vascular rinsing and chilling involves infusing a chilled isotonic solution (98.5% water and a blend of mono- and di-saccharides and phosphates) into the vasculature immediately upon exsanguination. Primary purposes of carcass vascular rinsing are to (1) effectively remove residual blood from the carcass; (2) lower internal muscle temperature rapidly; and (3) optimize pH decline by effective delivery of glycolytic substrates in the rinse solution. Previous studies have revealed that the beef carcass vascular rinsing early postmortem positively affects meat quality, product shelflife, and food safety. Thus, the objective of this review is to provide a more comprehensive understanding of the physical and biochemical mechanisms associated with beef carcass vascular rinsing, focusing on the relationship between quality attributes (CIE L^*, a^*, b^*; chemical states of myoglobin; oxygen consumption and sarcomere length) and muscle metabolic response to various substrate solutions (Rinse & Chill^®, fructose, sodium phosphate, and dipotassium phosphate) that stimulate or inhibit the rate of glycolysis early postmortem. In addition, this review discusses the absence of metabolite residues (phosphorus, sodium, and glucose) related to the application of the chilled isotonic solution. This review primarily focuses on beef and as such extending the understanding of the mechanisms and meat quality effects discussed to other species associated with vascular rinsing, in particular pork, may be limited.

• Journal of Animal Science and Technology
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• 제64권3호
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• pp.397-408
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• 2022

Rinse & Chill^® technology (RCT) entails rinsing the vasculature using a chilled isotonic solution (3℃; 98.5% water and a blend of dextrose, maltose, and sodium phosphates) to rinse out the residual blood from the carcass. Infusion of pre-chilled solutions into intact animal carcasses immediately upon exsanguination is advantageous in terms of lowering the internal muscle temperature and accelerating chilling. This technology is primarily used for purposes of effective blood removal, favorable pH decline, and efficient carcass chilling, all of which improve meat quality and safety. Although RCT solution contains some substrates, the pre-rigor muscle is still physiologically active at the time of early postmortem and vascular rinsing. Consequently, these substrates are fully metabolized by the muscle, leaving no detectable residues in meat. The technology has been commercially approved and in continuous use since 2000 in the United States and since 1997 in Australia. As of January 2022, 23 plants have implemented RCT among the 5 countries (Australia, US, Canada, New Zealand, and Japan) that have evaluated and approved RCT. All plants are operating under sound Sanitation Standard Operation Procedures (SSOP) and a sound Hazard Analysis Critical Control Point (HACCP) program. No food safety issues have been reported associated with the use of this technology. RCT has been adapted by the meat industry to improve product safety and meat quality while improving economic performance. Therefore, this review summarizes highlights of how RCT technically works on a variety of animal types (beef, bison, pork, and lamb).