• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.227-231
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• 1998

The teleosts represent ancient real-bony vertebrates in phylogeny and resemble major genetic patterns to higher vertebrates. In the present study, we have defined the single family of insulin-like growth factors (IGFs) from flounder (Paralichthys olivaceus), compared to the prototype of IGFs observed in the Agnathan hagfish. In flounder, IGFs are clearly diverged into two major types including type I and II, and they are structurally similar by displaying a multidomain structure consisting of five functional regions as previously found in other vertebrates. However, flIGF-I appears to be more basic (pI 8.03) than the flIGF-II (pI 5.34) in the fully processed form for the B to D domain region. The flIGF-I seems to contain an evolutionary conserved Asn-linked glycosylation in E domain, which is not found in flIGF­II. The most interesting feature is that flIGF-II appeared to be structurally close to hagfish IGF in secondary structures, particularly in Band D domains. This could tell us an idea on the molecular divergence of IGFs from the Agnatha to teleosts during the vertebrate phylogeny. It also support, in part, a notion regarding on how IGF-II is appeared as more embryonic during development. Nonetheless, the biologically active B to D domain region of flIGF-II shows significant sequence homology of $65.6\%$ to flIGF-Is and contains the evolutionary conserved insulin-family signature, as well as a reserved recognition site (Lys) in D domain, necessary to generate proteolytic cleavage for E-peptide. A significant structural difference was found in E domain in which flIGF-I possesses two potential alternative splicing donor site at $Val^{17,\;24}$ of E domain. Therefore, it seems so far that IGF-I sorely produces spliced variants due to the spliced E-peptide moiety while IGF-II appears to be maintained in a single type during evolution. IGF-II, however, may be also possible to transcribe unidentified variants, depending on the physiological conditions of tissues in vertebrates in vivo.

• Journal of Acupuncture Research
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• v.30 no.1
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• pp.1-11
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• 2013

Objectives : The 7-zone-diagnostic system is a device for predetermining bodily locations by measuring the energy of the living body. The purpose of this study is to examine correlations between gastroscopy results and zone-5 data from the 7-zone-diagnostic system. Methods : This study was carried out with data from gastroscopy procedures. It involved 115 patients who had been diagnosed with gastritis, esophagitis, or gastric ulcers. These patients were divided into three groups according to the different patterns of zone-5 factors AA, FL2 and FL1. Group A was made up of patients for whom the red bar graph of zone-5 was higher than the normal range for factor AA. Group B was made up of patients for whom the red bar graph of zone-5 was within the normal range for factor AA. Group C was made up of patients for whom the red bar graph of zone-5 was lower than the normal range for factor AA. Group D E and F were made up of same way as group A B and C for factor FL2. Group G H and I were made up of same way as group A B and C for factor FL1. After the collection of gastroscopy results and data on zone-5 from the 7-zone-diagnostic system, the data was analyzed statistically. Results : 1. Group D had a higher ratio of medication than group E, and this result was statistically significant. 2. Group D G had a higher ratio of medication than group E G and group E H, and this result was statistically significant. 3. Group G had a higher ratio of esophagitis than group H, and this result was statistically significant. 4. A Group B H had a higher ratio of esophagitis than group B G, group C G, and group C H, and this result was statistically significant. 5. The medication group had a high score on the function index than the non-medication group, and this result was statistically significant. Conclusions : This study suggests that there is a slight correlation between gastroscopy results and zone-5 data from the 7-zone-diagnostic system. The research resulted in significant data that are helpful for diagnosing digestive system problems through the use of the 7-zone-diagnostic system.

• Korean Journal of Community Nutrition
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• v.3 no.4
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• pp.594-608
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• 1998

Comparative studies regarding the nutritional status of 93 home-living elderly people taking free congregate lunch meals(FL) and 87 middle income class elderly people(MI) were performed in Taejon city. Data was obtained from questionaires, anthropometry and interviews for the 24-hour dietary recall of 2 nonconsecutive days during August, 1996. The average age for FL was 75.8 years. The monthly familly income for FL belonged to the low-income class. FL females had lower heights and weights than MI females. The average daily nutritional intake of both FL and MI were low, particularly in FL whose %RDA of energy was 68.5%, protein 65%, Ca 29.6%, Fe 50.8%, vitamin A 34.5%, vitamin E 30.5%, riboflavin 40.6%, vitamin C 76.9%. MI's %RDA of energy was 76.4%, protein 80.a2%, Ca 48.1%, Fe 78.6%, vitamin A 67.3%, vitamin E 117.4%, riboflavin 45.6%, vitamin C 136.5%. Comsumption of Zn, vitamin $B_6$ and folic daily average. There was no nutrient having average INQ(Index of nutritional quality) over 1 for either group. The INQs for protein, Ca, Fe and vitamin A were 0.802, 0.377, 0.625 and 0.296 in FL, and 0.900, 0.601, 0.784 and 0.602 in MI, respectively. The MAR(Mean adequacy ratio) was low with the value of 0.500-0.518 in FL and 0.630-0.723 in MI. The percentage of main nutrients from lunch was the highest among the three meals for FL males, while that from breakfast was the highest for MI. Free lunches taken by FL supported higher percentages of main nutrients than home-lunches taken by MI. Eating-out was done more frequently by MI than by FL and that eating-out brought them more nutritional intake. The above data indicated that the dietary nutritional intake status of the FL elderly was very poor in both quantity and quality and that free congregate lunch significantly contributed to the daily nutrient-intake for the FL elderly.

• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.113-118
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• 1999

The insulin-like growth factor (IGF) is found in all vertebrates and its type-II molecule is regarded as a fundamental embryonic growth factor during development. We have firstly identified, in this study, a cDNA clone corresponding to IGF-II (flIGF-II) from the adult brain of the teleost, Paralichthys olivaceus. We also examined the tissue expression of flIGF-II in several adult tissues by RT-PCR. The flIGF-II cDNA contained a complete ORF consisting of 215 amino acids and one stop codon. Its molecular characteristics appear to be similar to the previously identified IGF-II molecules, in which a common primary structure exhibiting B, C, A, D, and E domains is evidently observed. This cDNA clone seems to be cleaved at $Ala_{52}$ for the $NH_2$-end signal peptide and appears to produce a 98 amino acid-long E-peptide from the $Arg^{118}$. The functional B-D domain regions, therefore, include 65 amino acids and is able to encode a 7.4-kDa protein. The most prominent structural difference between IGF-I and IGF-II was that the D domain of IGF-II exhibits a two-codon-deleted pattern compared to the 8 amino acid-containing IGF-I. The insulin family signature in the A domain and six cysteins forming three disulfide bridges between the B and A domains were evolutionary-conserved from teleosts to mammalian IGF-II. Interestingly, the E-peptide region appears to provide a distinct hallmark between teleosts in amino acid composition. The flIGF-II shows 85.1% of sequence identity to salmon and trout, 90.6% to tilapia, and 98.4% to perch in amino acid level. In tissue expressions of IGF-II, it is very likely that flIGF-II has a significant expression in the adult brain. However, liver seems to be the main source for IGF-II production, and relatively low signals were observed in the adult muscle and kidney. Taken together, it would be concluded that the functional region for IGF-II mRNA is highly similar in phylogeny and is evolutionary, conserved as a mediator for the growth of vertebrates.

• Journal of Photoscience
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• v.5 no.3
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• pp.131-135
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• 1998

Using differential display reverse transcription technique, the present study attempted to isolate and characterize genes specifically expressed in cotyledons of Pharbitis nil Choisy cv. Violet during floral induction. A total of 107 bands specific to the inductive condition were initially obtained with 80 primer sets of 20 different arbitrary primers combined with 4 kinds of T12MN. In northern blot analysis with reamplified cDNAs as probes, three cDNAs were detected to be expressed specificcally in the induced cotyledon tissues, and designated PnFL-1, PnFL-2 and PnFL-3 , the size of which were 228 bp, 317 bp and 272 bp, respectively. A search for sequences similar to the decuced amino acid sequences was conducted using GenBank and EMBL database ; seequence encoded by PnFL-1 had 29% identity with the clone of Arabidopsis thaliana similiar to reverse trascriptase (Genbank Acc. N0.3047086), PnFL-2 shared 50% identity with hydroxiyproline-rich glycoprotein of Glycine max(GenBank Acc. No.347455), and PnFL-3 had 46% identity with TAMU 4. Thaliana genomic clone T23E16 (GenBank Acc. No.B67574). None of them was known gene in the plant system up to date, implying that the fragments may comprise parts of genes which are associated with the floral induction in Pharbitis nil.

• IMMUNE NETWORK
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• v.20 no.5
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• pp.42.1-42.10
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• 2020

Long-lasting post-switched plasma cells (PCs) arise mainly from germinal center (GC) reactions, but little is known about the mechanism by which GC B cells differentiate into PCs. Based on our observation that the expression of the transcription factor CCAAT/enhancer binding protein β (C/EPBβ) is associated with the emergence of post-switched PCs, we enquired whether a cell-autonomous function of C/EPBβ is involved in the program for PC development. To address this, we generated C/EPBβ-deficient mice in which the Cebpb locus was specifically deleted in B cells after transcription of the Ig γ1 constant gene segment (Cγ1). In response to in vitro stimulation, B cells from these Cebpb^fl/flCγ1^Cre/+ mice had defects in the induction of B lymphocyte-induced maturation protein 1 (Blimp1) and the formation of IgG1⁺ PCs, but not in proliferation and survival. At steady state, the Cebpb^fl/flCγ1^Cre/+ mice had reduced serum IgG1 titers but normal IgG2c and IgM titers. Moreover, upon immunization with T-dependent Ag, the mice produced reduced levels of Ag-specific IgG1 Ab, and were defective in the production of Ag-specific IgG1 Ab-secreting cells. These results suggest that a cell-autonomous function of C/EPBβ is crucial for differentiation of post-switched GC B cells into PCs through a Blimp1-dependent pathway.

• The Korean Journal of Food And Nutrition
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• v.22 no.2
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• pp.192-198
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• 2009

Cheongkukjang is a traditional fermented soybean food in Korea that is used in the manufacture of functional foods. This study was conducted to assess vitamin contents and sensory qualities of powdered Cheongkukjang dried by oven drying, far-infrared drying and freeze drying. Vitamins $B_1$, $B_2$, $B_6$, E, niacin and pantothenic acid were detected using all drying methods. However, vitamin D was not detected in the oven drying samples, and was most prevalent($82.47{\mu}g$/100 g) in the far-infrared drying samples. The sensory characteristics included color, off flavor, salty taste, bitter taste, sweet taste, roasted taste and after-taste. Oven drying and freeze drying samples exhibited highest and lowest color scores(5.62 and 2.0, respectively). Oven drying samples also exhibited the highest roasted taste score(4.76), although not significantly different from far-infrared drying samples(4.0, p<0.05), while the score of freeze drying samples(2.38) was significantly different from the other methods(p<0.05). It is concluded that freeze drying is most suitable.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.2
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• pp.153-155
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• 2001

Eight new three-way cross heterozygotic hybrid genotypes of the mulberry silkworm Bombyx mori, resulted from hybridization of multivoltine as female and bivoltine Fl hybrid as male component, have been evaluated for the cumulative effect of nine traits of commercial economic importance with the objective of selecting out the most prospective ones for their exploitation as an intermediate technology during unfavorable season. Five of them namely, G(P5${\times}$NBl8) (E.I. =57.23); S(NB18${\times}$P5) (E.I. : 55.48); S(P5${\times}$NBl8) (E.I. = 52.44); S(KBN${\times}$B7) (E.I. = 50.42) and S(NB7${\times}$KB) (E.I. : 50.16) have scored higher values of the Evaluation Index (E.I.) and could be selected in the same order for exploitation during unfavorable seasons in tropical climates.

• The Journal of Information Technology
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• v.10 no.4
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• pp.1-10
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• 2007

The C.B.D(Central Business District), called the central place of the urban area, is core of the city. It is consists of variety services. This study is aimed at analyzing the land use of the C.B.D. in Daejeon. Deajeon Metropolitan City site $36^{\circ}11'{\sim}36^{\circ}30'N,\;127^{\circ}15'{\sim}127^{\circ}34'E$. The field survey area is JoongAng-ro neighboring area, partial EunHaing-dong. The results are as follows: First, The land use of surveyed area is concentrate on variety services, because of excellent accessibility. Second, The land use of ground floor(Fl) and second floor(F2) has services for the customers convenience to approach. Third, The land use of surveyed area has undergone a lot of changes over the last three years(2004. 11~2007. 11.)

• The Journal of the Acoustical Society of Korea
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• v.19 no.5
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• pp.60-64
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• 2000

This paper studies the relation between the Fundamental Frequency (F0) and the formants of simple vowels in the Korean language sung by sopranos. It is hewn that, in soprano singing, the F0 of a vowel affects its formants. For this reason the formants of simple vowels sung by sopranos must be considered in all over the soprano singing range. We recorded the five simple vowel sounds /a/, /e/, /i/, /o/, and /u/ sung by five professional sopranos from A3 (220.0Hz) to A5 (880.0Hz) in the major scale and compared the formants of the sung vowels with those of spoken vowels. We observed that F1 and F2 of sung vowels were stable in low F0 (lower than B4) but in high F0 (higher than B4), F1 and F2 lost their stabilities. In the case of /a/, /o/, and /u/, the slope of the F1-F2 graph was about 2.6, and those of the F0-F2 and F0-Fl graphs were 2.2-2.5 and 0.7-1.0, respectively. And as the F0 increases, the F1 and F2 of sung vowels /a/, /e/, /i/, /o/, and /u/ were almost the same. At A5, the Fl and F2 of five sung vowels had the same values. This results suggest that the relation between the F0 and the formants be used to synthesize soprano's singing vowels.