CLONGING OF GENES EXPRESSED UPON FLORAL INDUCTION IN PHARBITIS COTYLEDONS

  • Published : 1998.10.01

Abstract

Using differential display reverse transcription technique, the present study attempted to isolate and characterize genes specifically expressed in cotyledons of Pharbitis nil Choisy cv. Violet during floral induction. A total of 107 bands specific to the inductive condition were initially obtained with 80 primer sets of 20 different arbitrary primers combined with 4 kinds of T12MN. In northern blot analysis with reamplified cDNAs as probes, three cDNAs were detected to be expressed specificcally in the induced cotyledon tissues, and designated PnFL-1, PnFL-2 and PnFL-3 , the size of which were 228 bp, 317 bp and 272 bp, respectively. A search for sequences similar to the decuced amino acid sequences was conducted using GenBank and EMBL database ; seequence encoded by PnFL-1 had 29% identity with the clone of Arabidopsis thaliana similiar to reverse trascriptase (Genbank Acc. N0.3047086), PnFL-2 shared 50% identity with hydroxiyproline-rich glycoprotein of Glycine max(GenBank Acc. No.347455), and PnFL-3 had 46% identity with TAMU 4. Thaliana genomic clone T23E16 (GenBank Acc. No.B67574). None of them was known gene in the plant system up to date, implying that the fragments may comprise parts of genes which are associated with the floral induction in Pharbitis nil.

Keywords

References

  1. Ann. Rev. Plant Physiol. Plant Mol. Biol. v.39 The control of floral evocation and morphogenesis Bernier, G.
  2. J. Exp. Bot. v.35 Phloem exudate of Perilla crispa and its effects of flowering of flowering of P. crispa shoot explants Purse, J. G.
  3. Plant Cell Physiol. v.31 Flowerinducing activity of phloem exudate in cultured apices from Pharbitis seedings Ishioka, N.;S. Tanimoto;H. Harada
  4. Plant Cell Physiol. v.32 Flowerinducing activity of phloem exudates from Pharbitis cotyledons exposed to various photoperiods Ishioka, N.;S. Tanimoto;H. Harada
  5. J. Plant Biol. v.37 Effects of electric current on flowering in Pharbitis and floral stimulus activity in the phloem exudate of cotyledons Kim, S. H.;M. W. Yi;J. Maeng
  6. J. Plant Biol. v.39 Flowerinducing activity in the phloem exudate and gene expression specific to photoperiodic floral induction in Pharbitis cotyledons Kim, K. C.;J. H. Lee;Y. Hur;J. Maeng
  7. Physiol. Plant. v.61 Changes in the composition of in vitro translated leaf mRNA caused by photoperiodic flower induction of Hyoscyamus niger Warm, E.
  8. Planta v.189 A complex pattern of changes in polysomal mRNA populations is evident in the leaves of Arabidopsis thaliana (L.) Heynh. during photoperiodic induction of flowering Lechner, F. J.;W. Rau
  9. Plant Cell Physiol. v.17 Qualitative analysis by isoelectric focusing of the protein content of Pharbitis nil apices and cotyledons during floral induction Stiles, J. I.;P. J. Davies
  10. Korean J. Bot. v.4 Immunological analysis of proteins in the leaf of Pharbitis nil during photoinduction of flowering Maeng, J.
  11. Plant Physiol. v.84 Changes in cotyledon mRNA during ethylene inhibition of floral induction in Pharbitis nil strain Violet Lay-Yee, M.;R. M. Sachs;M. S. Reid
  12. Planta v.171 Changes in cotyledon mRNA during floral induction of Pharbitis nil cv. Violet Lay-Yee, M.;R. M. Sachs;M. S. Reid
  13. Plant Growth Reg. v.10 Polypeptide profiles from cotyledons of developing and photoperiodically induced seedlings of the Japanese morning glory (Pharbitis[Ipomoea]nil) Bassett, C. L.;C. P. Mothershed;G. A. Galau
  14. Sci. in China v.34 Specific protein occurrence related with photoperiodic flower induction in the cotyledons of Pharbitis nil cv. Violet Li, L. W.;K. H. Tan
  15. Plant Sci. v.78 Changes in in vitro translated polypeptides of Pharbitis nil Chois., strain Violet cotyledons during the first flower-inductive and non-inductive photoperiods Ono, M.;K. Yamada;K. Sage;M. Okazaki;H. Harada
  16. Plant Sci. v.89 Changes in polypeptides in Pharbitis cotyledons during the first flower-inductive photoperiod Ono, M.;K. Sage-Ono;M. Yasui;M. Okazaki;H. Harada
  17. Plant Cell Physiol. v.37 Transient increase in the level of mRNA for a germinlike protein in leaves of the short-day plant Pharbitis nil during the photoperiodic induction of flowering Ono, M.;K. Sage-Ono;M. Inoue;H. Kamada;H. Harada
  18. Plant Cell Physiol. v.39 Dark-induced accumulation of mRNA for a homolog of transcriptionally controlled tumor protein (TCTP) in Pharbitis Sage-Ono, K.;M. Ono;H. Harada;H. Kamada
  19. Plant Physiol. v.116 Accumulation of a clock-regulated transcript during flower-inductive darkness in Pharbitis nil Sage-Ono, K.;M. Ono;H. Harada;H. Kamada
  20. Plant Physiol. v.100 Structure and expression of a heat-shock protein 83 gene of Pharbitis nil Felsheim, R. F.;A. Das
  21. Plant Physiol. v.100 Structure and light-induced expression of a small heat-shock protein gene of Pharbitis nil Krishna, P.;R. F. Felsheim;J. C. Larkin;A. Das
  22. Plant Physiol. v.104 Dark and circadian regulation of mRNA accumulation in the short-day plant Pharbitis nil O'Neill, S. D.;X. S. Zhang;C. C. Zheng
  23. Science v.257 Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction Liang, P.;A. B. Pardee
  24. Anal. Biochem. v.162 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chlorcform extraction Chomczynski, P.;N. Sacchi
  25. Molecular Cloning: A Laboratory Manual (2nd ed.) Sambook, J.;E. F. Fritsch;T. Maniatis
  26. Plant Physiol. v.94 A flower-inducing substance of high molecular weight from higher plants Takeba, G.;Y. Nakajima;A. Kozaki;O. Tanaka;Z. Kasai
  27. Plant Physiol. v.95 A polypeptide that induces flowering in Lemna paucicostata at a very low concentration Kozaki, A.;G. Takeba;O. Tanaka