• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.140-144
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• 2003

Type Ⅰ signal peptidase is an integral membrane protein that functions to cleave signal peptides from secreted and membrane proteins. The enzyme serves as a potential target for the development of novel antibacterial agents due to its unique physiological properties. Despite being one of the best characterized enzymes, the catalysis of Type Ⅰ signal peptidase still remains controversy over the catalytic serine/lysine dyad mechanism. It appears that the dyad proteases are generally less efficient than the prototypical serine/histidine/aspartic acid triad found in most enzymes, although Type Ⅰ signal peptidase is an exception to this rule. In this paper, we have proposed that Type Ⅰ signal peptidase may act as the serine/lysine/aspartic acid triad cataltytic mechanism. Therefore, the aspartic acid 99 residue in the E. coli signal peptidase was chosen and mutated to an alanine to see if there is any possible role of the aspartic acid in the catalytic function. Type Ⅰ signal peptidase D99A protein was inactive in vitro assay using the procoat synthesized by in vitro transcription translation. However, the mutant was active using a highly sensitive in vivo assay. Pulse-chase experiments show that the replacement of aspartic acid 99 with alanine results in a very unstable signal peptidase molecule. Therefore, we conclude that it is unlikely that the residue is directly involved in catalysis, but rather plays an important role in stabilizing the protein structure.

• Korean Parent-Child Health Journal
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• v.3 no.2
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• pp.49-66
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• 2000

Pregnancy is a task of creation in which a women mobilizes her self and the resources available to her in the generation of a new person. Through the pregnancy, a mother has formed the new human relationship with a fetus. Maternal-fetal relationship is considered one of mechanism making the relationship of mother and child. It is important to well-being of mother and fetus, too. The earliest interaction between a mother and her child is during prenatal period. Maternal-fetal dyad is unique and perceived interactions with the fetus make the pregnancy real for the mother. Maternal behavior is "instinctive" and is formed in early childhood by copy of the mother. But, Rubin argues that this behavior is an open intellectual system rather than a prepackaged bundle of traits. There is openness to new learning and a high value placed on knowing which occurs with silent organization in thought. Thus, nurses and other health professionals provide prenatal care that optimally is part of the environment in which the maternal-fetal dyad develops. Thus it is appropriate for nurses to increases their understanding of the dyad and to explore ways to enhance its development. This study focusses on the interaction ability and response of fetus, and the maternal-fetal interaction. The research of fetal responses that involve physiological changes and motor movement have been shown to coccur to both external sensory stimuli and to maternal emotional states. The fetus does also have sensory capacity to be aware of some maternal behaviors, and the motor ability to respond in a way the mother can notice. Thus, very rudimentary interactions appear to be possible. Maternal awareness of fetal activity was supported by several studies. More interesting to the present study are description of maternal-fetal interaction and the finding that there appear to be levels of sensitivity to the fetus involved in maternal-fetal interactions. First, recognition comes that the fetus is separate from the maternal self. Next, the fetus engages in. Lastly, the parent may describe active interaction with the fetus, believing that mother and fetus are communicating on a meaningful level. Several interventions, developed to promote more active interaction between mother and fetus, have been reviewed. In general, the parents were taught to stimulate the fetus and to notice the fetus' responses. This type of intervention might increase the mother's sensitivity to her unborn baby, and she may have a head start toward learning how to res pond sensitivity to the newborn infant. Research In the area of maternal-fetal interaction is scarce. Sensitive behavior is construed as an appropriate and timely response to a signal of need from another person, but no such signal of need can be claimed regarding the fetus. The highest level of maternal-fetal interaction, therefore, might be based more on maternal representations of the imagined fetus than on factual evidence of fetal participation.

• Proceedings of the Costume Culture Conference
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• 2003.04a
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• pp.53-54
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• 2003

본 연구의 목적은 Thibaut과 Kelley(1959)의 상호의존이론(a theory of interdependence)을 중심으로 가족 구성원 중 가장 밀접한 어머니와 딸을 dyads로 하여 의복의 선호와 소유 사이의 관계를 아동과 청소년 연령집단을 대상으로 알아보는 것이다. 피험자는 대전지역에 거주하는 초등학생(75명)과 고등학생(64명), 그리고 그들의 어머니(139명)로 편의 표집 방법에 의해 선정되었으며, 자료수집은 설문지법에 의하여 이루어졌다. 의복의 측정 도구는 의복범주(2)×디자인의 복잡성(2)×여향성ㆍ남향성(2)의 요인설계로 하여 24종류의 의복 자극물로 구성되었으며, 자극물에 대한 타당성은 전문가 집단과의 토론 및 예비조사를 통해 검증하였다. (중략)

• Analytical Science and Technology
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• v.7 no.1
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• pp.91-102
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• 1994

The monomer compositions in a series of propylene heterophasic copolymer, propylene random copolymer, propylene random terpolymer and ethylene-propylene-ENB terpolymer have been determined from $^{13}C-NMR$ spectra. The simplified and highly resolved $^{13}C-NMR$ spectra made it possible to assign unambiguousely and calculate the monomer composition. A complete sets of NMR chemical shift assignments and the way to measure the quantity of monomer are newly given in diverse polymers. Furthermore complete dyad, triad, tetrad and pentad distributions have been able to be determined. These NMR quantitative analytical results for monomer compostition have consistent with those from Infrared spectral data.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.12
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• pp.128-137
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• 2018

Drawing on costly signaling theory and self-enhancement motive, this study examines the relationships among proactive personality, knowledge sharing behavior, and organizational recognition. In addition to the individual characteristic, this study considers job characteristics as conditional factors, and especially proposes the moderated mediation model in which job complexity and variety moderate the relationships among proactive personality, knowledge sharing behavior, and organizational recognition. To prove these hypotheses, empirical analyses are conducted with 166 dyad samples collected from various industries. As predicted, individuals with high proactive personality are more likely to become involved in knowledge sharing behavior, and this behavior increases organizational recognition rated by their supervisors. Moreover, job complexity and variety moderate the positive relationship between proactive personality and organizational recognition is mediated by knowledge sharing behavior. These results reveal the motive in knowledge sharing and the boundary condition that is necessary to increase such behavior. The study findings will ultimately contribute theoretical and empirical implications to the knowledge management literature.

• Journal of Oriental Neuropsychiatry
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• v.31 no.4
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• pp.269-278
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• 2020

Objectives: Yukmijihwang-won (Liuweidihuang-wan in Chinese) is a frequently used medicinal herbal formula. It is used as Yin tonic in Korea and China to recover patients from Yin deficiency. However, the scientific evidence on this drug has not revealed the beneficial effect or mechanism of its effects on the neurological disorder. We designed this study to examine the antidepressive and analgesic effects of Yukmijihwang-won (YJ-01) and the minor modification of YJ-01, YJ-06 on the reserpine-induced pain-depression dyad mice model. Methods: Reserpine (1 mg/kg) was administered subcutaneously once a day for three consecutive days to induce pain and depression-like behavior. The oral administration of YJ-01 and YJ-06 (100, 200, or 300 mg/kg) was performed once daily from three days after the reserpine injection. Results: Repeated administration of the YJs significantly reduced the immobility time in a forced swimming test and increased the moved distance and number of crossings in the open field test. In the von-Frey filament test, the oral administration of YJs remarkably suppressed the increase in paw withdrawal frequency. Conclusions: The results of this study suggest that YJ-01 and 06 may be good candidates to treat the pain-depression dyad.

• Journal of Photoscience
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• v.9 no.2
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• pp.347-349
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• 2002

Synthetic zinc chlorins possessing a hydrophylic polyoxyethylene chain at the 17 -position were prepared. An amphiphilic zinc chlorin possessing a single chIorin moiety showed absorption maxima at 675 nm in an aqueous medium, indicating that the zinc chIorin did not form large aggregates but a dimeric structure. In contrast, amphiphilic zinc chlorin dyads in which two zinc chlorin moieties were connected with a hydrophilic polyoxyethylene linkage showed red-shifted absorption band around 720-740 nm in an aqueous medium. The result indicated that the amphiphilic zinc chlorin dyad self-aggregated to form chlorosome-like oligomer.

• Korean Journal of Child Studies
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• v.20 no.1
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• pp.147-164
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• 1999

The purpose of this study was to investigate (1) intergenerational transmission from mother's internal working model of relationships formed in her childhood and infant's attachment patterns and (2) the associations among maternal working models, maternal sensitivity, and infant attachment patterns. The subjects were twenty 12- to 20-month-old infants and their mothers. The Adult Attachment Interview (1996) was used to assess mother's internal work model of relationships. Interviews were audio-recorded and transcribed verbatim. Each dyad was videotaped in the modified Ainsworth Strange Situation(1978) and filmed for 3 minutes in the Questionaire Situation of Smith and Pederson (1988). Mothers were classified as autonomous(F), dismissing(Ds), preoccupied(E), or unresolved/disorganized(Ud). Infants were classified as secure(B), insecure-avoidant(A), or insecure-resistant(C). Exact A/B/C/D and Ds/F/E/Ud agreement was observed in 55% of the dyads. Maternal sensitivity was related to infant attachment patterns but not to maternal working models.

• Korean Journal of Child Studies
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• v.24 no.3
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• pp.15-26
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• 2003

This research investigated young children's social interaction and task performance in dyadic collaboration. The independent variables were gender and social skill level(high/low groups). Twenty-four pairs each 4-year-old boys and 4-year-old girls(total 96 subjects) worked at a block reconstruction. Two researchers judged degree of dyadic collaboration. Data were processed by 2 way ANOVA and Pearson's product correlation. While no main effect for gender was found, the effect for social skill level(high/low groups)was significant for social interaction. There was positive correlation between social interaction and task performance.

• Korean Journal of Child Studies
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• v.31 no.1
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• pp.219-234
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• 2010

This study examined the influence of mothers' reading and scaffolding behaviors on children's emotional experiences and memory recall during picture book reading. Sixty children, aged 6 and their mothers participated in this study. Each child-mother dyad was observed in a lab setting which was designed to evaluate child-mother interactions during a picture book reading. The data were analyzed using correlation and multiple regression techniques. Our results indicated that mothers' task-oriented reading behavior affected the children's lack of positive emotions, whereas mothers' fun-oriented reading behavior was associated with the children's positive emotions. The task-oriented reading behavior also accounted for children's poor memory performance in relation to the book. These results were discussed in terms of the relationship between emotion and cognition through book reading experiences.