• 한국작물학회지
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• 제36권3호
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• pp.280-285
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• 1991

우리나라 고대농업과 기술을 형성하는 데 직접간접으로 기간역할을 해 왔던 고대중국의 도작기술은, 이미 한전농법과 농본관(정신)을 기원전에 완성하였으며, 특히 심경과 정수 및 토양비옥도 유지를 위한 경전법을 중심으로 도작기술이 발전되었고, 따라서 이앙법, 정조식 심종법, 철제농기구 사용, 수리를 위한 피당 축조기술이 앞섰고, 다양한 지역에서 재해를 회피하거나 다양한 용도의 품질공급을 위하여 벼 품종의 분화가 잘이루어져 왔다. 또한 중국농업의 발상지가 화북이었기 때문에 인희지광의 특성에 맞는 조방적 도작기술로서 화경수누법과 세역법이 태동되었고, 한무제와 ‘참위’학자들의 음역오행설이 농업관과 연계되면서 파종이나 작목선택에 대한 기술이 만술적으로 발달한 경향도 있었다. AD 6세기경의 $\ulcorner$제민요술$\lrcorner$부터 14세기의 왕복 $\ulcorner$농서$\lrcorner$에 이르기까지 도작기술은 지속적, 상가적으로 분화발달되어 왔으나 원대 이후에는 정체된 경향이었다. 이 시점에서 우리나라의 여말 농업과 $\ulcorner$농사직설$\lrcorner$시대가 시작되었다.

• 한국콘텐츠학회논문지
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• 제19권10호
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• pp.487-510
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• 2019

본 연구는 윤리적 리더십이 갖고 있는 양면성에 초점을 맞추어, 리더의 윤리적 리더십이 직무열의, 조직몰입, 비윤리적 친-조직행동에 미치는 영향을 검증하고, 직무열의와 조직몰입의 이중 매개효과를 분석하였다. 이를 위해 565부의 설문지를 활용하여 연구를 수행한 결과는 다음과 같다. 첫째, 리더의 윤리적 리더십은 비윤리적 친-조직행동에 정(+)의 영향을 미치지 않은 것으로 나타났다. 둘째, 직무열의는 리더의 윤리적 리더십과 비윤리적 친-조직행동의 관계에서 매개역할을 하지 않았으나, 조직몰입은 매개역할을 하는 것으로 나타났다. 셋째, 리더의 윤리적 리더십과 비윤리적 친-조직행동의 관계에서 직무열의와 조직몰입은 이중 매개역할을 하는 것으로 나타났다. 시사점은 다음과 같다. 본 연구는 윤리적 리더십이 항상 긍정적인 효과만을 발휘하는 것은 아니며, 비윤리적 행동과의 관계성을 규명했다는 데 의의가 있다. 또한 조직 내 구성원의 인지, 정서, 태도, 행동의 과정을 살펴보고, 다시 한 번 정서사건이론의 패턴을 확인할 수 있었다. 나아가 이러한 행동패턴이 사회교환이론에 의해 규명되어진다는 점을 밝히는데 이론적 함의를 찾을 수 있다. 실무적 시사점은 조직차원에서 이러한 부정적인 영향 및 가능성을 미연에 방지하고 나아가 사회적 차원에서 조직성과를 제고하는 동시에 윤리적 기준을 준수할 수 있는 조직문화 및 환경을 조성할 수 있도록 적극적인 노력이 필요하다는 시사점을 제공하고 있다.

• 한국균학회지
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• 제46권4호
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• pp.479-490
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• 2018

고추의 주요 병해충인 총채벌레(Thrips palmi)와 탄저병(Colletotrichum acutatum)을 방제하기 위해 다양한 화학농약이 사용되고 있지만, 농약의 오남용으로 인하여 이에 대한 저항성이 발생하여 방제가 힘들어지고 환경오염을 초래하는 문제가 있다. 그리하여 본 연구에서는 이러한 문제를 극복하고자 총채벌레에 대한 살충효과와 탄저병에 항균력이 우수한 균주를 선발하여 동시 방제의 가능성을 확인하고자 하였다. 토양으로부터 분리한 곤충병원성 곰팡이 13균주($1{\times}10^7conidia/mL$)를 오이총채벌레 성충과 약충에 처리하여 70~100%의 우수한 살충률을 나타내는 6균주를 선발하였다. 선발한 6균주의 항균활성을 검정하기 위하여 고추 탄저병균에 대치 배양한 결과, 항균력이 우수한 FT333 균주를 최종 선발할 수 있었다. 최종 선발된 균주를 형태학적 조사와 internal transcribed spacer, ${\beta}-tubulin$ 영역의 염기서열 분석을 통하여 Isaria javanica로 동정하였고, I. javanica FT333으로 명명하였다. I. javanica FT333 (KACC93316P)의 고추 탄저병균에 대한 방제 효과를 기내에서 검정한 결과($1{\times}10^5$, $1{\times}10^6$, $1{\times}10^7conidia/mL$) 95% 이상의 방제효과를 나타내었다. 이러한 결과들을 바탕으로 I. javanica FT333 균주가 고추 탄저병균과 미소해충인 오이총채벌레를 동시에 방제하기 위한 미생물제제로서 이용될 수 있는 가능성을 제시하였다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.104.1-104
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• 2003

To screen for potential biocontrol agents against postharvest disease of garlics caused by Penicillium hirsutum, a total of 933 isolates (432 fungi and 501 bacteria) were isolated from the rhizoshere or rhizoplane of garlics. Among them, Pantoea agglomerans isolate 59-4 (Pa 59-4) was selected for a potential biocontrol agent by in vivo wounded garlic bulb assay, When the spore suspension (10$\^$5/ spores/$m\ell$) of Penicillium hirsutum was co-inoculated with spore or cell suspension of each fungal or bacterial isolate on wounded garlics, the isolate highly suppressed disease development. Soaking garlic bulbs in the suspension of Pa 59-4 significantly reduced garlic decay from p. hirsutum. However, Pa 59-4 did not inhibit the mycelial growth of P. hirsutum in dual-culture with P. hirsutum on Tryptic soy agar. In order to elucidate mode of action of Pa 59-4 nutrient competition between Pa 59-4 and P. hirsutum was investigated using tissue culture plates with cylinder inserts containing defusing membrane reported by Janisiewicz et al. The results showed that Pa 59-4 effectively suppressed spore germination and mycelial growth of blue mold in the low concentration (0.5％) of garlic juice, but did not suppress those of blue mold in the higher concentration (5％) of garlic juice. This result suggests that the mechanism in biocontrol of garlic blue mold by Pa 59-4 may involve in nutrient competition with P. hirsutum on garlic bulbs.

• The Plant Pathology Journal
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• 제22권1호
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• pp.103-106
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• 2006

In this biocontrol research, we evaluated disease suppressive effects of antagonistic bacterial strains ISE13 and KJ1R5 against Korean ginseng root rot caused by P. eaetorum. We also examined the effects of nutrient solution in the hydroponic culture system for Korean ginseng on biological activity of the bacterial strains. As results of dual culture tests of the bacterial strains on $V_{8}$ juice agar, the strain ISE13 showed antifungal activity against P. eaetorum and other plant pathogenic fungi, but the strain KJ1R5 did not. When their inhibitory effects against infection of P. eaetorum on the roots grown in either nutrient solution or water were tested, the strains ISE13 and KJ1R5 inhibited the disease severity of Korean ginseng roots only grown with water, compared to buffer-treated, inoculated controls. However, the nutrient solution used for hydroponic cultures of ginseng in pots caused higher levels of disease severity by the strains ISE13 and KJ1R5 from 418.8\%$ to 40.0\%$ and from 24.3\%$ to 45.0\%$, respectively. In this study, the bacterial strains ISE13 and KJ1R5 could be potentially biocontrol agents to suppress Korean ginseng root rot caused by P. eaetorum. However, more attention using nutrient solution in hydroponic cultures for Korean ginseng production should be applied in biocontrol of plant diseases using the antagonistic microorganisms.

• 한국균학회지
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• 제19권3호
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• pp.231-237
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• 1991

참깨 시들음병과 역병에 대한 괄항균(括抗菌) Streptomyces spp.의 길항현상과 생물학적 방제 효과를 구명코져 참깨 시들음병(F. oxysporum f. sp. vasinfectum)과 역병(P. nicotianae var. parasitica)이 발생한 포장에 이병주로 부터 병원균을 순수분리(純粹分離)하였고 괄항균(括抗菌)은 충북일원의 고추, 참깨포장의 72점의 흙으로 부터 공시 병원균에 효과있는 괄항균(括抗菌)을 대치배양법(對峙培養法)으로 선발하였다. 선발된 괄항균(括抗菌) St-11 과 St-20 을 공시하여 병원균에 대한 균사저지원, 괄항균(括抗菌) 배양 여액에서의 병원균의 생육저지, 발아억제 빛 lysis 를 관찰 조사하였고 괄항균(括抗菌) 현독액(縣獨液 )(St-11) 을 종자에 처리할 경우 참깨생육에 아무런 영향이 없었다. 온실에서 괄항균(括抗菌)의 토양접종에 의한 참깨시들음병과 역병에 대한 방제효과는 병원균 단독 접종구에 비하여 40-78% 의 방제효과를 보였다.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1527-1535
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• 2018

Bacterial strain BAS23 was isolated from rice field soil and identified as Bacillus amyloliquefaciens. Based on dual culture method results, the bacterium BAS23 exhibited potent in vitro inhibitory activity on mycelial growth against a broad range of dirty panicle fungal pathogens of rice (Curvularia lunata, Fusarium semitectum and Helminthosporium oryzae). Cell-free culture of BAS23 displayed a significant effect on germ tube elongation and mycelial growth. The highest dry weight reduction (%) values of C. lunata, H. oryzae and F. semitectum were 92.7%, 75.7%, and 68.9%, respectively. Analysis of electrospray ionization-mass spectrometry (ESI-MS) and $^1H$ nuclear magnetic resonance (NMR) spectroscopy revealed that the lipopeptides were iturin A with a C14 side chain (C14 iturinic acid), and a C15 side chain (C15 iturinic acid), which were produced by BAS23 when it was cultured in nutrient broth (NB) for 72 h at $30^{\circ}C$. BAS23, the efficient antagonistic bacterium, also possessed in vitro multiple traits for plant growth promotion and improved rice seedling growth. The results indicated that BAS23 represents a useful option either for biocontrol or as a plant growth-promoting agent.

• 건축역사연구
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• 제11권2호
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• pp.23-35
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• 2002

Even though there have been greate changes in the types and contents of the housing plan in Korea since the Modem age, it seems that the identity of traditional dwelling culture is still remains within it. The purpose of this study is to examine the characteristics and features in the development of the 'Public Standard' Housing Unit Plan by comparing the differences and similarities in unit plans in housings built on Japanese occupation period and those built after the period. From the study, we can conclude as follows: 1. The public standard housing unit plans on both periods have shown the common features in the composition of their physical forms in the way that the front-row area still remains the traditional housing culture and the rear-row area reflects the desires of the modem lives. The structure of the dual-row unit plan appeared in the public standard housing plan was caused by the cultural acculturation in Japanese occupation period. 2. The public standard housing unit plans, however, show the differences in the contents and meaning in units plans with the them of the Japanese housing because of the cultural differences. These differences took placed autonomously through the change of housing plans in the modern time, and have the common features such that 1) the changes in the Maru-centered plan type differ in composition from the inner-corridor plan type used Japanese housing, and 2) the composition of the open-inner spaces in the double-row housing plan reflects the single-row outer open space In the traditional housing. All of these cultural progresses in the public standard housing unit plans was took place as the result of the inner-oriented and notional circulations derived from the modernity. However, the transformed unit plans still contains the identity derived from the variation of the traditional Korean building elements such as Maru and Ondol. In other words, those are the Maru-centered and composed housing plans with juxtaposition of the inner block and the outer block. As the conclusion, though the public standard dwelling unit plan has the similarity in the physical form, it is different in the cultural Identity and meaning from the public standard plans before the independence from the Japan occupation.

• Asian-Australasian Journal of Animal Sciences
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• 제28권5호
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• pp.620-631
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• 2015

A total of 59 lactic acid bacteria (LAB) strains were isolated from corn stover silage. According to phenotypic and chemotaxonomic characteristics, 16S ribosomal DNA (rDNA) sequences and recA gene polymerase chain reaction amplification, these LAB isolates were identified as five species: Lactobacillus (L.) plantarum subsp. plantarum, Pediococcus pentosaceus, Enterococcus mundtii, Weissella cibaria and Leuconostoc pseudomesenteroides, respectively. Those strains were also screened for antimicrobial activity using a dual-culture agar plate assay. Based on excluding the effects of organic acids and hydrogen peroxide, two L. plantarum subsp. plantarum strains ZZU 203 and 204, which strongly inhibited Salmonella enterica ATCC $43971^T$, Micrococcus luteus ATCC $4698^T$ and Escherichia coli ATCC $11775^T$ were selected for further research on sensitivity of the antimicrobial substance to heat, pH and protease. Cell-free culture supernatants of the two strains exhibited strong heat stability (60 min at $100^{\circ}C$), but the antimicrobial activity was eliminated after treatment at $121^{\circ}C$ for 15 min. The antimicrobial substance remained active under acidic condition (pH 2.0 to 6.0), but became inactive under neutral and alkaline condition (pH 7.0 to 9.0). In addition, the antimicrobial activities of these two strains decreased remarkably after digestion by protease K. These results preliminarily suggest that the desirable antimicrobial activity of strains ZZU 203 and 204 is the result of the production of a bacteriocin-like substance, and these two strains with antimicrobial activity could be used as silage additives to inhibit proliferation of unwanted microorganism during ensiling and preserve nutrients of silage. The nature of the antimicrobial substances is being investigated in our laboratory.

• 한국식물병리학회지
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• 제14권5호
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• pp.445-451
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• 1998

A severe Phytophthora rot of strawberry caused by a species of Phytophthora has been widely occurred at major cultivation areas of Kimhae on August in 1997. Incidence of the disease was obtained in the range of 69.2~83.6% in surveyed 4 fields and showed an average of 75.2%. A species of Phytophthora was mostly isolated from the crown of infected strawberry plants and all the isolates were identified as P. nicotianae var. nicotianae (=P. parasitica). The fungus showed strong pathogenicity on strawberry by inoculation test. As a result of the leaf inoculation using mycelial disks of the fungus, both leaves and petioles were darkly browned, and were finally blighted. As a result of the root inoculation of zoospore suspension, both roots and crowns were rotten with dark brown. Although the fungus produced sporangia either on V-8 juice agar medium or liquid medium, the sporangia observed on the liquid medium appeared to be broadly turbinate and noncaducous. Moreover the fungus cultured on the liquid medium often produced sporangia having two papilla. The number of zoospores in sporangia was found to be ranged from 3 or 4 to as many as 20 or 25. In addition, the released zoospore from the sporangium became the cystospore during the prolonged culture of the fungus. The sporangia were measured as av. 49$\times$35 ${\mu}{\textrm}{m}$ with l/b ratio of 1.43. All isolates from crowns were heterothallic and A1 mating type since oospores were abundantly formed on clarified V-8 juice agar by dual culture with P. capsici A2 mating type. Aplerotic oospores were sized 24-26 ${\mu}{\textrm}{m}$. Antheridia were always amphigynous and recoreded an average of 12$\times$10 ${\mu}{\textrm}{m}$. Hyphal swlling were easily observed, and terminal or intercalary chlamydospores were abundantly formed on V-8 juice agar as well as in C/Z solution and sized av. 28.2 ${\mu}{\textrm}{m}$. This is the first report of Phytophthora rot of strawberry in Korea.