• Title/Summary/Keyword: dual culture

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Dual Mode Feedback-Controlled Cycling System for Upper Limb Rehabilitation of Children with Cerebral Palsy

  • Cho, Seung-Yeon;Kim, Jihun;Seo, Seong-Won;Kim, Sung-Gyung;Kim, Jaehyo
    • International Journal of Advanced Culture Technology
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    • v.7 no.1
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    • pp.231-236
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    • 2019
  • Background/Objectives: This paper proposes a dual mode feedback-controlled cycling system for children with spastic cerebral palsy to rehabilitate upper extremities. Repetitive upper limb exercise in this therapy aims to both reduce and analyze the abnormal torque patterns of arm movements in three- dimensional space. Methods/Statistical analysis: We designed an exercycle robot which consists of a BLDC motor, a torque sensor, a bevel gear and bearings. Mechanical structures are customized for children of age between 7~13 years old and induces reaching and pulling task in a symmetric circulation. The shafts and external frames were designed and printed using 3D printer. While the child performs active/passive exercise, angular position, angular velocity, and relative torque of the pedal shaft are measured and displayed in real time. Findings: Experiment was designed to observe the features of a cerebral palsy child's exercise. Two children with bilateral spastic cerebral palsy participated in the experiment and conducted an active exercise at normal speed for 3 sets, 15 seconds for each. As the pedal reached 90 degrees and 270 degrees, the subject showed minimum torque, in which the child showed difficulty in the pulling task of the cycle. The passive exercise assisted the child to maintain a relatively constant torque while visually observing the movement patterns. Using two types of exercise enabled the child to overcome the abnormal torque measured in the active data by performing the passive exercise. Thus, this system has advantage not only in allowing the child to perform the difficult task, which may contribute in improving the muscle strength and endurance and reducing the spasticity but also provide customizable system according to the child's motion characteristic. Improvements/Applications: Further study is needed to observe how passive exercise influences the movement characteristics of an active motion and how customized experiment settings can optimize the effect of pediatric rehabilitation for spastic cerebral palsy.

Entomopathogenic Fungi as Dual Control Agents against Both the Pest Myzus persicae and Phytopathogen Botrytis cinerea

  • Yun, Hwi-Geon;Kim, Dong-Jun;Gwak, Won-Seok;Shin, Tae-Young;Woo, Soo-Dong
    • Mycobiology
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    • v.45 no.3
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    • pp.192-198
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    • 2017
  • The green peach aphid (Myzus persicae), a plant pest, and gray mold disease, caused by Botrytis cinerea, affect vegetables and fruit crops all over the world. To control this aphid and mold, farmers typically rely on the use of chemical insecticides or fungicides. However, intensive use of these chemicals over many years has led to the development of resistance. To overcome this problem, there is a need to develop alternative control methods to suppress populations of this plant pest and pathogen. Recently, potential roles have been demonstrated for entomopathogenic fungi in endophytism, phytopathogen antagonism, plant growth promotion, and rhizosphere colonization. Here, the antifungal activities of selected fungi with high virulence against green peach aphids were tested to explore their potential for the dual control of B. cinerea and M. persicae. Antifungal activities against B. cinerea were evaluated by dual culture assays using both aerial conidia and cultural filtrates of entomopathogenic fungi. Two fungal isolates, Beauveria bassiana SD15 and Metarhizium anisopliae SD3, were identified as having both virulence against aphids and antifungal activity. The virulence of these isolates against aphids was further tested using cultural filtrates, blastospores, and aerial conidia. The most virulence was observed in the simultaneous treatment with blastospores and cultural filtrate. These results suggest that the two fungal isolates selected in this study could be used effectively for the dual control of green peach aphids and gray mold for crop protection.

A Study on the Field Survey of the Korean Communities' Dwelling Houses of Rural Districts in East-Northern China - Longshancun, Yanbian - (동북지역 농촌의 조선민족 살림집의 실태조사 연구 -연변 룡정시 룡산촌을 중심으로-)

  • Rhee, Shin Ho;Kim, June Bong
    • Journal of the Korean Institute of Rural Architecture
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    • v.4 no.1
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    • pp.29-36
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    • 2002
  • The characteristics of the rural dwelling houses of the Korean communities were investigated and analyzed to understand their rural dwelling culture in east-northern China. This research was done by the field survey of rural village, Longshancun, Yanbian. The structures of their houses are changed into the brick houses from the earthen houses with wood frame. The floor plans are changed into Dual-bay-type of Divided-space from Single-bay-type of Hollistic-space, the largest ratio at present, and the numbers of space are increased. In the view of total outline, their dwelling houses are based to those of east-northern Korea and are sustained to the vernacular dwelling styles(ex : floor heating, ondol, and sit-down), and are adapted to the geography and climate of Yanbian and accepted to the culture of other communities(ex : indoor-kitchen, indoor-removing-shoes).

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First Report: Diversity of Endophytic fungi Possessing Antifungal Activity Isolated from Native Kougoed (Sceletium tortuosum L.)

  • Sishuba, Anathi;Leboko, Jessica;Ateba, Collins Njie;Manganyi, Madira Coultyne
    • Mycobiology
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    • v.49 no.1
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    • pp.89-94
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    • 2021
  • Forty-three (n = 43) endophytic fungi with different morphologic characteristics were from a medicinal plant Sceletium tortuosum, were utilized to investigate their antifungal effectiveness against pathogenic fungi. All fungal isolates exhibited antifungal activity against one or more pathogens in the dual culture test whereas only 33 fungal culture filtrates (77%) showed decent antifungal effect. Fusaria and Aspergillus were the dominate genus that displayed significant antifungal activity. Isolates GG02, GG09, ND15, and ND17 showed the broadest spectrum of antifungal activity. Furthermore, culture filtrate of Fusarium sp. DR08 exhibited a broad range of antifungal activity against all the pathogens. The results suggest endophytic fungi isolated from medicinal plant might be a source of novel bioactive molecules. To the best our knowledge, this is the first report on endophytic fungi isolated from native kougoed exhibiting antifungal activity against plant fungal pathogens.

Study on the Korean application of cultural accessibility and cultural arts education: focusing on French cultural policy

  • Hanbyul Kim;Soelah Kim
    • Analyses & Alternatives
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    • v.8 no.1
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    • pp.33-57
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    • 2024
  • This study examines the main elements, concepts, and directions of 'cultural accessibility' in the field of cultural arts education through the French case. Above all, 'cultural accessibility' is viewed from the perspective of the problems of 'social exclusion' and the corresponding 'participation'. We discuss strategies for applying 'cultural accessibility' through French policy discussions. France is implementing this consistent policy structure through the development of its local self-government system. We first look at the current status of French cultural policy and cultural accessibility, and the participation. Next, we will discuss separately into the cultural arts education of the central government and those of local governments. For application to Korea, we propose the application of education to apply 'culture of public values', the establishment of autonomy in cultural arts education, and the application of a dual strategy for public advocacy.

The Analysis of Leisure Time Use of Urban Dual-earner Couples with Preschool Children (미취학 자녀를 둔 도시 맞벌이 부부의 여가시간에 관한 연구)

  • Han, Young-Sun;Yoon, So-Young
    • Journal of the Korean Home Economics Association
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    • v.47 no.2
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    • pp.81-95
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    • 2009
  • The purpose of this study is to examine gender equity in the quantity and flow experience of leisure time of urban dual-earner couples having preschool children. The quantity of leisure time is measured by primary activity and the flow experience of leisure time is measured by primary and secondary activities. The data collected by Korean National Statistical Office in 2004 is used. The sample for this study consists of 255 couples, and the statistical methods are frequency, percentage, paired t-test, and one-way ANOVA. The following is a summary of the major findings. The first, the pattern of the leisure time was different between husbands and wives. The quantity of leisure time of husbands was longer than that of wives. The husbands spent more time to perform social activities, media contact, sports and outdoor activities, while women did more in religious activities. The second, husband's leisure flow experience was higher than that of wives. The pure leisure time of wives was shorter than that of husbands and wives experienced more contaminated leisure time by a secondary activity. In other words, wives's leisure is more likely to be interrupted, to involve episodes of shorter duration, and to be associated with personal care and unpaid work.

Development of Two Quantitative Real-Time PCR Diagnostic Kits for HPV Isolates from Korea

  • Jeeva, Subbiah;Kim, Nam-Il;Jang, In-Kwon;Choi, Tae-Jin
    • Journal of Microbiology and Biotechnology
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    • v.22 no.10
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    • pp.1350-1358
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    • 2012
  • Viral pathogens, alongside other pathogens, have major effects on crustacean aquaculture. Hepatopancreatic parvovirus (HPV) is an emerging virus in the shrimp industry and has been detected in shrimp farms worldwide. The HPV genome has greater diversity than other shrimp viruses owing to its wide host range and geographical distribution. Therefore, developing diagnostic tools is essential to detect even small copy numbers from the target region of native HPV isolates. We have developed two easy to use quantitative real-time PCR kits, called Green Star and Dual Star, which contain all of the necessary components for real-time PCR, including HPV primers, using the primers obtained from the sequences of HPV isolates from Korea, and analyzed their specificity, efficiency, and reproducibility. These two kits could detect from 1 to $1{\times}10^9$ copies of cloned HPV DNA. The minimum detection limits obtained from HPV-infected shrimp were $7.74{\times}10^1$ and $9.06{\times}10^1$ copies in the Green Star and Dual Star assay kits, respectively. These kits can be used for rapid, sensitive, and efficient screening for HPV isolates from Korea before the introduction of postlarval stages into culture ponds, thereby decreasing the incidence of early development of the disease.

Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases

  • Ji, Seung Hyun;Paul, Narayan Chandra;Deng, Jian Xin;Kim, Young Sook;Yun, Bong-Sik;Yu, Seung Hun
    • Mycobiology
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    • v.41 no.4
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    • pp.234-242
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    • 2013
  • A total of 62 bacterial isolates were obtained from Gomsohang mud flat, Mohang mud flat, and Jeju Island, Republic of Korea. Among them, the isolate CNU114001 showed significant antagonistic activity against pathogenic fungi by dual culture method. The isolate CNU114001 was identified as Bacillus amyloliquefaciens by morphological observation and molecular data analysis, including 16SrDNA and gyraseA (gyrA) gene sequences. Antifungal substances of the isolate were extracted and purified by silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. The heat and UV ray stable compound was identified as iturin, a lipopeptide (LP). The isolate CNU114001 showed broad spectrum activity against 12 phytopathogenic fungi by dual culture method. The semi purified compound significantly inhibits the mycelial growth of pathogenic fungi (Alternaria panax, Botrytis cinera, Colletotrichum orbiculare, Penicillium digitatum, Pyricularia grisea and Sclerotinia sclerotiorum) at 200 ppm concentration. Spore germ tube elongation of Botrytis cinerea was inhibited by culture filtrate of the isolate. Crude antifungal substance showed antagonistic activity against cucumber scleotiorum rot in laboratory, and showed antagonistic activity against tomato gray mold, cucumber, and pumpkin powdery mildew in greenhouse condition.

Isolation and Biocontrol Potential of Bacillus amyloliquefaciens Y1 against Fungal Plant Pathogens

  • Jamal, Qaiser;Lee, Yong Seong;Jeon, Hyeon Deok;Park, Yun Suk;Kim, Kil Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.5
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    • pp.485-491
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    • 2015
  • This study was performed to investigate thermophilic bacteria from soil having broad antifungal spectrum against Rhizoctonia solani, Colletotrichum gloeosporioides, Phytophthora capsici, Fusarium oxysporum f.sp. lycopersici, and Botrytis cinerea. One isolate selected could resist heat shock of $60^{\circ}C$ for one hour, and had broad antifungal activity in dual culture assay against all tested fungal pathogens and was identified as Bacillus amyloliquefaciens Y1 using 16S rRNA gene sequence. Further investigation for antifungal activity of bacterial culture filtrate (BCF) and butanol crude extract (BCE) of various concentrations showed broad spectrum antifungal activity and fungal growth inhibition significantly increased with increasing concentration with highest growth inhibition of 100% against R. solani with 50% BCF and 11 mm of zone of inhibition against R. solani with 4 mg BCE concentration. Treatment of butanol crude extract resulted in deformation, lysis or degradation of C. gloeosporioides and P. capsici hyphae. Furthermore, B. amyloliquefaciens Y1 produced volatile compounds inhibiting growth of R. solani (70%), C. gloeosporioides (65%) and P. capsici (65-70%) when tested in volatile assay. The results from the study suggest that B. amyloliquefaciens Y1 could be a biocontrol candidate to control fungal diseases in crops.

Paradox and Irony in Postmodern Picturebook - With Focus on Piggybook by Anthony Browne (포스트모던 그림책에서의 역설과 아이러니 - 앤서니 브라운의 『돼지책』을 중심으로)

  • Ra, Sunhee
    • Cross-Cultural Studies
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    • v.38
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    • pp.65-87
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    • 2015
  • This study has examined, first of all, one of unique characteristics of picturebook, so-called dual audience. To be more exact, this paper aimed at exploring the flexibility of picturebooks that allows both of children and adult reader to enjoy them. For this, we analysed Piggybook by Anthony Browne that so far has been read as a traditional picturebook with a single distinctive narrative rather than as a postmodern picturebook with multiple narratives, centering on paradox and irony. The results of this study were as fallows: Piggybook has the potential of multiple readings and meanings for a variety of audiences. Above all, this picturebook raises not only the question about the gender roles but also the one about representation of reality through the language.