• The Korean Journal of Food And Nutrition
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• v.22 no.4
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• pp.696-700
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• 2009

The adaptability of dry rehydratable film for the qualitative evaluation of coliform bacteria and Escherichia coli was tested. In general, culture methods that employ lactose broth or desoxycholate lactose agar are used for qualitative tests of coliform bacteria. Culture using lactose broth showed a high detection yield and low selectivity when compared to the dry rehydratable film. However, culture methods that employ lactose broth required a long time(48 hrs) for qualitative tests of coliform bacteria and complicated procedures were required to prepare the medium. The detection of coliforms using desoxycholate lactose agar had a slightly higher selectivity than the dry rehydratable film method, but this difference was not statistically significant. However, the preparation of the desoxycholate lactose agar was complicated. EC broth for the detection of E. coli showed the highest detection yield and lowest selectivity; however, this method required complicated procedures for preparation of the medium as well. Overall, the dry rehydratable film had a slightly lower detection yield than the other methods. The detection yield of dry rehydratable film method was over 37.1% at a concentration of 1 cfu/$m{\ell}$. Additionally, the dry rehydratable film method showed high selectivity and did not require preparation. However, because the selectivity of the dry rehydratable film was high, it took a long time(36 hrs) to detect E. coli. Overall, these findings indicate that dry rehydratable film can be used for qualitative detection of coliforms and E. coli.

• Journal of Food Hygiene and Safety
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• v.10 no.1
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• pp.41-43
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• 1995

Dry rehydratable film (Petrifilm) method was compared with the standard pate count (SPC) method for estimation of total bacteria in ginseng products. Ginseng products (7 sample) was analyzed for total count by the SPC, and Petrifilm methods, respectively. In the case of ginseng tea, ginseng extract, ginseng extract pill, ginseng powder capsule, and ginseng extract tea, they showed non-significant values at the 1% level. However, the values of ginseng powder and tablet showed significant at the 1% level. These results generally indicate the suitability of the dry rehydratable film methods as alternatives to the SPC method for estimating of total bacteria in ginseng product samples except to ginseng powder and ginseng tablet.

• Journal of Korean Society on Water Environment
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• v.36 no.2
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• pp.148-152
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• 2020

The quantitative accuracy of dry rehydratable film method was compared with the standard culture method to analyze the applicability of dry rehydratable film in the total aerobic bacteria test for water. The materials used in this study were 500 cases of water and were tested according to the Korean official test method for drinking water. The mean value of the total aerobic bacteria in plate count agar(PCA) and MC-Media Pad AC(MAC) were 2.6 ± 1.1 Log cfu/mL and 2.6 ± 1.2 Log cfu/mL. The p value and correlation coefficient(R²) were 0.933 and 0.9985 between the PCA and MAC, respectively, indicating no significant difference and very high correlation. According to the water types, the p value and R² were 0.887 and 0.9911 in the PCA and MAC. In the purified water, the p value was 0.973 and R² was 0.9934 in the PCA and MAC of other water. In each type of water, there were no significant differences between the PCA and MAC, and the correlation was very high. Thus, it is suggested that the MAC would be available for total aerobic bacteria test for water.

• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.294-300
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• 2005

Contents of total aerobic bacteria, coliform, Escherichia coli, yeast, mold, and Staphylococcus aureus in meat, dairy, and fishery products were analyzed by dry rehydratable film method using 3M $Petrifilm^{TM}$ and compared against those obtained through conventional method. Two methods showed high correlations of 0.990-0.999, 0.975-0.999, 0.979-0.987, 0.978-0.984, and 0.999 for total aerobic bacteria, yeast and mold, coliform, E. coli, and S. aureus, respectively; therefore, dry rehydratable film method using 3M $Petrifilm^{TM}$ offers acceptable alternative to conventional method for enumeration of microorganisms in meat, dairy, and fishery products.

• Journal of Food Hygiene and Safety
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• v.19 no.4
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• pp.209-216
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• 2004

Dry rehydratable film methods were compared to conventional methods for the enumeration of microorganisms in Korean traditional foods. Kimchi, doenjang, kochujang, kanjang, takju, sujeongkwa and sikhe were used as Korean traditional foods. $Petrifilm^{TM}$ aerobic count plate, $Petrifilm^{TM}$ coliform count plate, $Petrifilm^{TM}$ E. coli/coliform count plate, $Petrifilm^{TM}$ yeast and mold count plate and $Petrifilm^{TM}$ staph express count plate were compared to plate count agar, most probable number (MPN) for coliform, MPN for E. coli, potato dextrose agar and coagulase test, respectively. Regression analysis indicated that correlation coefficient values were 0.974-0.998, 0.913-0.995, 0.955-0.978, 0.968-0.986 and 0.998-0.999 for total aerobic bacteria, yeast and mold, coliform, E. coli and S. aureus, respectively. There were no significant differences between two methods, suggesting that $Petrifilm^{TM}$ plates can be used as an alternative to conventional method for the determination of microorganisms in Korean traditional foods.