To study the relationship between certain hormones and metabolites and between hormones and milk yield during different stage of lactation, six lactating Karan Swiss cows and six Murrah buffaloes were maintained. Growth hormone, insulin, $T_3$, $T_4$, glucose, BHBA, NEFA and milk yield were studied. Highly negative relationship of growth hormone with insulin and triiodothyronine in cows and marginally negative in buffaloes suggest that insulin and triiodothyronine aid in the process of partitioning of nutrients towards milk production through reducing the demands of nutrients by peripheral tissue. The significant and negative correlation of growth hormone with dry matter intake in both the species suggest that the availability of nutrients from the digestive tract play a role in the regulation of growth hormone secretion. Positive relationship of growth hormone with non esterified fatty acids in both the species suggest that high growth hormone levels may result in fat mobilization and thereby increase the availability of energy precursors for milk synthesis. Insulin was negatively correlated with milk yield and lactose content and positively with milk fat and protein but the degree of relationship varied. In both the species the relationship between triiodothyronine and milk yield was negative and between thyroxine and milk yield was positive. However, it was significant only in cows and not in buffaloes. Thyroxine was positively correlated with beta-hydroxybutyrate and non-esterified fatty acids with milk yield in both the species.
Khan, M. Ajmal;Sarwa, M.;Nisa, Mahr-Un;Khan, M. Sajjad
Asian-Australasian Journal of Animal Sciences
/
v.17
no.8
/
pp.1093-1097
/
2004
This study was aimed to establish the amount of enzose (corn dextrose) for optimum N fixation in urea treated corncobs (UTC) and their dietary effect on nutrient intake, digestibility and milk yield in crossbred cows. Corncobs were treated with 5% urea and ensiled in laboratory silos with 0, 2, 4 and 6% enzose for 15 days. Total nitrogen (N), neutral detergent fiber (NDF) and neutral detergent insoluble N contents were increased in UTC with the level of enzose. Five early lactating crossbred cows (Sahiwal$\times$Holstein Frisian) were used in a 5$\times$5 Latin Square Design to see the influence of UTC ensiled with 6% enzose on nutrient intake, digestibility, milk yield and its composition. Five iso-nitrogenous and iso-caloric diets were formulated. The UTC30 (control), UTC40, UTC50, UTC60 and UTC70 diets contained 30% UTC ensiled without enzose and 40, 50, 60, 70% UTC ensiled with 6% enzose, respectively. Dry matter, NDF and ADF intakes were increased with the increasing level of UTC ensiled with enzose in the diets of cows. Dry matter, NDF and ADF digestibilities were significantly higher with diets containing UTC ensiled with enzose. Milk yield was significantly higher in cows fed UTC70 compared to those fed on other diets. The milk crude protein percentage was significantly different across treatments. However, milk fat, total solids, solid not fat, true protein and non-protein nitrogen contents of milk remained similar across all diets. Ensilation of UTC with 6% enzose improved the nitrogen retention and thus enhanced the feeding value of UTC for lactating cows.
Mui, Nguyen Thi;Ledin, Inger;Uden, Peter;Binh, Dinh Van
Asian-Australasian Journal of Animal Sciences
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v.15
no.1
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pp.45-54
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2002
Ninety lactating goats (Bachthao, Barbary, Beetal and Jamnapary breeds) were used in an experiment to investigate the replacement value of the tree fodders Flemingia (Flemingia macrophylla) and Jackfruit (Artocarpus heterophyllus). The foliages were used to replace the concentrate in diets based on chopped whole sugar cane (Sacharatum sp.), Para grass (Brachiaria mutica) and dried cassava root (Manihot esculanta). The concentrate was replaced by foliage of Jackfruit or Flemingia at 0%, 20%, 40%, 60% and 80% based on the crude protein (CP) content in the concentrate and foliages, respectively. Average milk yield was 1,617 g/day for goats fed Jackfruit compared to 1,532 g/day for those fed Flemingia. Increasing amounts of Flemingia foliage resulted in reduced dry matter intake and decreased milk yield but milk composition (CP, casein and fat content) was similar up to 60% replacement. Flemingia showed a poor potential as a supplement for lactating goats and replacement levels should not exceed 20% of the protein in the concentrate or 7.5% of the dry matter in the diet. With respect to the combination of milk production and net return over the control a CP replacement rate of 20% was the most promising. For Jackfruit there was similar feed intake and milk yield at a replacement level of 20% of CP in the concentrate (9.2% of DM intake) compared to the control diet. Milk yield at the level of 40% replacement (15% of the DM) in the diet was slightly reduced. Up to a level of CP replacement rate of 60% (21% DM in the diet) can be suggested for on-farm testing as a higher net return over the control was obtained on station.
In this study, occurrence of aflatoxin M$_1$(AEM$_1$) in domestic milk and milk products was determined. The level of AFM$_1$ in market milk (0.047 ppb) was lower than that in raw milk (0.083 pub) but this looks like that is due to dilution in collecting process rather than the effect of sterilization. In the case of nonfat dry milk, level of AFM$_1$appeared high by 0.24 ppb but it is thought to be not different from market milk actually because nonfat dry milk is diluted at intake. In the case of ice cream, finished products were contaminated with AFM$_1$of 0.020 ppd and also have the possibility of the contamination of AFB$_1$due to secondary raw material such as nuts and almond. On the basis of the results of this study and previous studies, Monte-Carlo simulation is conducted to estimate the contamination level of AFM$_1$in domestic market milk. To consider uncertainty and variability fitting procedure was passed through. And we used beta distribution to estimate the prevalence and triangular distribution to estimate the concentration level of AFM$_1$in milk. As a result, the 5%, 50% and 95% points of the distribution of the probability of AFM$_1$contamination level in milk is 0.0214, 0.0946 and 0.1888 ppb, respectively. Also we estimate that AFM$_1$in almost milk was low more than 0.5 ppb that is American acceptable level but 80.4% exceeded far 0.05 ppb that is European standard.
The aim of the present study was to determine the effect of dietary cultured wild ginseng root (CWGR) supplementation on goat milk composition and ginsenoside profiles. Sixteen Saanen dairy goats were allocated to two balanced groups based on lactation period, body weight ($38.6{\pm}3.2kg$), and dairy milk yield ($2.85{\pm}1.2kg$), and were kept in separate pens. Goats were fed a total mixed ration (TMR) feed (2.3 kg/d, dry matter basis) and 1.5 g of CWGR powder was supplemented in the experimental diet. The total feeding period was 3 weeks, and milk and blood samples were collected on the last three days of the experimental period. There was no effect of CWGR on daily milk yield and milk composition (fat, protein, lactose, and solid-not-fat). However, the CWGR-treatment group had significantly higher plasma IgG and protein contents than the control group (P < 0.05). Significant amounts of ginsenosides were observed in the milk of the CWGR-treatment group, whereas ginsenosides were not detected in the milk of the control group. In conclusion, dietary CWGR was a useful regimen to produce functional goat milk enriched in ginsenosides.
Crossbred dairy does were fed a roughage diet (IVOMD 56%, N 2.27%) ad libitum, and supplemented with urea-molasses (3% W : W) (UM) at levels on an air dry basis of 1.5% or 3% of liveweight or an iso-energetic, iso-nitrogenous mixture of cottonseed meal and molasses (25 : 75 w : w) (CM). Eight does, four lower-producers and four higher-producers (1.9 and 2.5 kg/day respectively in week 7 of lactation) arranged in two Latin Squares, received each of the four diets for three weeks. Dry matter, digestible organic matter and N intakes were higher for high-producers and high levels of supplement but did not differ between nitrogen sources. Milk production was higher by high-producers; interactions were significant between level of supplement and production group and between level of supplement and N-source, with maximum production by high producers on high levels of CM. The main effects of level of supplement were only significant for production or composition. and total solids; N-source did not have significant effects on liveweight, milk production or composition. We conclude that does of moderate capacity for milk production, receiving a diet of two-thirds moderate quality roughage, one third urea-molasses, will not respond to increased level of supplementation or to replacement of urea with cottonseed meal.
Journal of The Korean Society of Grassland and Forage Science
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v.20
no.4
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pp.251-258
/
2000
A field experiment was conducted to evaluate agronomic characteristics, forage quality and dry matter yield of Korean native wild hairy vetch(Vicia villosa Roth) and introduced vetch(Vicia) cultivars from Australia. Seeds were sown on 29 August 1998 and 29 September 1999 at the Livestock Experiment Farm, Keimyung College, Kyeongsan, Kyeongbuk. Vetch plants were harvested on 14 November 1998 and 5 May 1999 and 29 April 2000. 'Haymaker plus' and 'Cappelo woolly pod' (Vicia villosa ssp. dasycarpa(Ten.) Eav.) were higher than 'Namoi' (Vicia villosa ssp. dasycarpa(Ten.) Eav.) in winter survival and 'Popany' (Vicia benghalensis L.) and 'Victa' (Vicia sativa L.) did not overwinter from 1998 to 1999. 'Haymaker plus', 'Cappelo woolly pod', 'Namoi', Korean native wild hairy vetch and Chinese milk vetch were overwintered from 1999 to 2000, except 'Aneto common' (Vicia sativa L.). Fifty percent flowering was observed 23 April for 'Namoi', 24 April for 'Cappelo woolly pod' and 26 April for 'Haymaker plus' from 1998 to 1999. In 1999~2000, fifty percent flowering was observed 12 April for 'Namoi' and 'Cappelo woolly pod', 14 April for 'Haymaker plus', 23 April for Chinese milk, 30 April for 'Aneto common' and Korean native wild hairy vetch. Dry matter yield per hectare was significantly higher(p<0.05) for 'Haymaker plus', 'Cappelo' and 'Namoi' than 'Popany' and 'Vicata' from 1998 to 1999. In 1999-2000, dry matter yield per hectare was higher(p<0.05) for 'Namoi', 'Haymaker plus', 'Cappelo woolly pod' and Korean native wild hairy vetch than Chinese milk vetch (Astragalus sinicus L.) and 'Aneto common'. CP content was relatively high, but Chinese milk vetch and 'Aneto common' were lower than other cultivars. ADF(acid deterguent fiber) was low in Chinese milk vetch, but between the other cultivars were similar. According to the results obtained from this study, it is suggested that 'Haymaker plus' would be recommendable for fall sown vetch for dry matter production.
We investigated the effect of the amount of body condition loss in the dry period and early lactation in 42 high-yielding Holstein-Friesian cows on milk yield and the share of fatty acids in milk fat. Energy reserves were estimated based on the body condition scoring (BCS) and backfat thickness (BFT). Milk yield and milk composition were determined over 305-d lactation. From d 6 to 60 of lactation, the concentrations of 43 fatty acids in milk fat were determined by gas chromatography. Cows were categorized based on body condition loss from the beginning of the dry period to the lowest point of the BCS curve in early lactation into three groups: low condition loss group (L) ${\leq}0.5$ points (n = 14); moderate condition loss group (M) 0.75 to 1.0 points (n = 16) and high condition loss group (H) >1.0 points (n = 12). Cows whose body energy reserves were mobilized at 0.8 BCS and 11 mm BFT, produced 12,987 kg ECM over 305-d lactation, i.e. 1,429 kg ECM more than cows whose BCS and BFT decreased by 0.3 and 5 mm, respectively. In group H, milk yield reached 12,818 kg ECM at body fat reserve mobilization of 1.3 BCS and 17 mm BFT. High mobilization of body fat reserves led to a significant (approx. 5%) increase in the concentrations of monounsaturated fatty acids-MUFA (mostly $C_{18:1}$ cis-9, followed by $C_{18:1}$ trans-11), a significant decrease in the levels of fatty acids adversely affecting human health, and a drop in the content of linoleic acid, arachidonic acid and docosahexaenoic acid in milk fat. In successive weeks of lactation, an improved energy balance contributed to a decrease in the concentrations of unsaturated fatty acids (UFA) and an increase in the conjugated linoleic acid (CLA) content of milk fat.
The objective of this study was to determine the effects of linseed oil or whole linseed supplementation on performance and milk fatty acid composition of lactating dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The treatments consisted of basal ration (53:47; forage:concentrate ratio, on a dry matter [DM] basis, respectively) supplemented with 300 g/d of palm oil as a positive control diet (PO), or supplemented with 300 g/d of linseed oil (LSO), or supplemented with 688 g/d of top-dressed whole linseed (WLS). All cows were received ad libitum grass silage and individually fed according to the treatments. The experiment lasted for 10 weeks including the first 2 weeks as the adjustment period, followed by 8 weeks of measurement period. The results showed that LSO and WLS supplementation had no effects on total dry matter intake, milk yield, milk composition, and live weight change; however, the animals fed WLS had higher crude protein (CP) intake than those fed PO and LSO (p<0.05). To compare with the control diet, dairy cow's diets supplemented with LSO and WLS significantly increased milk concentrations of cis-9,trans-11-conjugated linoleic acid (CLA) (p<0.05) and n-3 fatty acids (FA) (p<0.01), particularly, cis-9,12,15-C18:3, C20:5n-3 and C22:6n-3. Supplementing LSO and WLS induced a reduction of medium chain FA, especially, C12:0-C16:0 FA (p<0.05) while increasing the concentration of milk unsaturated fatty acids (UFA) (p<0.05). Milk FA proportions of n-3 FA remarkably increased whereas the ratio of n-6 to n-3 decreased in the cows supplemented with WLS as compared with those fed the control diet and LSO (p<0.01). In conclusion, supplementing dairy cows' diet based on grass silage with WLS had no effect on milk yield and milk composition; however, trans-9-C18:1, cis-9,trans-11-CLA, n-3 FA and UFA were increased while saturated FA were decreased by WLS supplementation. Therefore, it is recommended that the addition 300 g/d of oil from whole linseed should be used to lactating dairy cows' diets.
A $2{\times}3$ factorial design was used to study the impact of rearing systems, individual (I) vs. group (G) and different levels of milk/skim milk feeding (three schedules, F1, F2 and F3) on performance of crossbred (Bos indicus ${\times}$ Bos taurus) calves. Six calves (three from each sex) were taken in each group on the basis of their birth weight. All the calves were fed colostrum for three days and thereafter, were allotted to three different milk feeding schedules (F), i.e. milk fed upto 8 weeks of age (F1), milk upto 4 weeks followed by 50% (F2) and 100% (F3) replacement of milk with skim milk in the next 4 weeks. Calf starter and cereal green fodder were fed ad libitum to all the calves beginning from second week of age. A digestibility trial was conducted at 15th week of age to assess nutrient utilization during postweaning period. The digestibilty of dry matter (DM), organic matter, total carbohydrate, ether extract and crude protein (CP) were nonsignificant between the rearing systems and the feeding schedules. There was significantly higher digestibility of NDF and ADF in G than I and in F3 than F1 and F2. The concentration CP and total digestible nutrients of the diet ranged from 17.18 to 17.75% and 66.32 to 70.14%, respectively. The DM intake (kg/100 kg body weight) ranged from 1.74 to 2.14 kg during 0 to 8 weeks and 3.19 to 3.41 kg during 0 to 14 weeks of age. The effects during postweaning phase (9-14 weeks of age) showed increased performance in group housed calves compared to individually housed ones with a superior average daily gain (590 vs. 443 g) and dry matter intake (1.79 vs. 1.64 kg). Above all, replacement of milk with skim milk at 50% level after 4th week followed by complete removal after 6th week of age (F2) seemed to suit better in coping with immediate energy starvation due to sole feeding of skim milk (F3) and they performed the best under group housed system of rearing.
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