• Journal of Nutrition and Health
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• 제24권5호
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• pp.431-441
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• 1991

To investigate the influence of age and dietary fat level on the immune function and the growing potential of the fibroblast cells, male rats of 2 month, 6 month and 30 month of age were fed either 6% or 30% fat diet for 16 weeks. The weight of thymus decreased linearly with increasing age. And this age-dependent degeneration of thymus was delayed in rats fed low fat diets. The blastogenesis of spleen lymphocytes to PHA, ConA, and PWM was decreased with increasing age, however, no effect of dietary fat level was observed. The age-related decline in ratios of PHA/ConA response may suggest that T suppressor cell activity increases with age. In cell culture system, lung fibroblast cells from 30M rats showed lower plating efficiency. longer doubling time. and shorter cumulative doubling potential than those from 2M or 6M animals. Also. the morphology of fibroblasts from 30M rats was tended to be rouned rather than flattened and more variable in size and being generally larger. wherease those from 2M and 6M rats were uniform in size and adhered tightly to the culture vessel in ordered arrays. Therefore fibroblast cell culture system tried in this study reflects the changes of cellular aging.

• Biomaterials and Biomechanics in Bioengineering
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• 제3권2호
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• pp.105-114
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• 2016

The purpose of this study is to evaluate the effects of cryopreservation on dental pulp-derived stem cells (DPSC) viability over a period of three years. Dental pulp-derived stem cells were isolated and cultured from thirty-one healthy teeth. DPSC isolates were assessed for doubling-time and baseline viability prior to cryopreservation and were assessed again at three time points; one week (T1), 18 months (T2), and 36 months (T3). DPSC can be grouped based on their observed doubling times; slow (sDT), intermediate (iDT), and rapid (rDT). Viability results demonstrated all three types of DPSC isolates (sDT, iDT and rDT) exhibit time-dependent reductions in viability following cryopreservation, with the greatest reduction observed among sDT-DPSCs and the smallest observed among the rDT-DPSC isolates. Cryopreserved DPSCs demonstrate time-dependent reductions in cellular viability. Although reductions in viability were smallest at the initial time point (T1) and greatest at the final time point (T3), these changes were markedly different among DPSC isolates with similar doubling times (DTs). Furthermore, the analysis of various DPSC biomarkers - including both intracellular and cell surface markers, revealed differential mRNA expression. More specifically, the relative high expression of Sox-2 was only found only among the rDT isolates, which was associated with the smallest reduction in viability over time. The expression of Oct4 and NANOG were also higher among rDT isolates, however, expression was comparatively lower among the sDT isolates that had the highest reduction in cellular viability over the course of this study. These data may suggest that some biomarkers, including Sox-2, Oct4 and NANOG may have some potential for use as biomarkers that may be associated with either higher or lower cellular viability over long-term storage applications although more research will be needed to confirm these findings.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.644-648
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• 2001

The biological control potential of entomopathogenic nematodes (EPN) can be enhanced by improved culture efficiency. Optimization of the media is a key factor for improving in vitro mass production of entomopathogenic nematodes. This study reports the effect of medium concentration. The medium is a combination of carbohydrates, lipids, proteins, sats, and growth factors, on the growth of Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus liminescens. The overall optimal medium concentration for nematode recovery, hermaphrodite size, bacterial mass, infective juveniles (IJs) yield, and doubling time was 84 g/l. At this concentration rate, the doubling time of IJs production and the biomass of symbiotic bacteria was 1.6 days and 12.8 g/l, respectively. The maximum yield of $2.4{\times}{10^5}IJs/ml$ was attained within a one-generation cycle (eight days). The yield coefficient was $2.8{\times}{10^6}$ IJs/g medium, and the maximum productivity was $3.1{\times}{10^7}$ IJs per day. Medium concentration affected two independent factors, recovery and hermaphrodite size, which in turn influenced the final yield.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4807-4814
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• 2012

Purpose: The chemoresistance of human hepatocellular carcinoma (HCC) to cytotoxic drugs, especially intrinsic or acquired multidrug resistance (MDR), still remains a major challenge in the management of HCC. In the present study, possible mechanisms involved in MDR of HCC were identified using a 5-fluorouracil (5-FU)-resistant human HCC cell line. Methods: BEL-7402/5-FU cells were established through continuous culturing parental BEL-7402 cells, imitating the pattern of chemotherapy clinically. Growth curves and chemosensitivity to cytotoxic drugs were determined by MTT assay. Doubling times, colony formation and adherence rates were calculated after cell counting. Morphological alteration, karyotype morphology, and untrastructure were assessed under optical and electron microscopes. The distribution in the cell cycle and drug efflux pump activity were measured by flow cytometry. Furthermore, expression of potential genes involved in MDR of BEL-7402/5-FU cells were detected by immunocytochemistry. Results: Compared to its parental cells, BEL-7402/5-FU cells had a prolonged doubling time, a lower mitotic index, colony efficiency and adhesive ability, and a decreased drug efflux pump activity. The resistant cells tended to grow in clusters and apparent changes of ultrastructures occurred. BEL-7402/5-FU cells presented with an increased proportion in S and G2/M phases with a concomitant decrease in G0/G1 phase. The MDR phenotype of BEL-7402/5-FU might be partly attributed to increased drug efflux pump activity via multidrug resistance protein 1 (MRP1), overexpression of thymidylate synthase (TS), resistance to apoptosis by augmentation of the Bcl-xl/Bax ratio, and intracellular adhesion medicated by E-cadherin (E-cad). P-glycoprotein (P-gp) might play a limited role in the MDR of BEL-7402/5-FU. Conclusion: Increased activity or expression of MRP1, Bcl-xl, TS, and E-cad appear to be involved in the MDR mechanism of BEL-7402/5-FU.

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.588-595
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• 2013

Mesenchymal stem cells (MSCs) are often known to have a therapeutic potential in the cell-mediated repair for fatal or incurable diseases. In this study, canine umbilical cord MSCs (cUC-MSCs) were isolated from umbilical cord matrix (n = 3) and subjected to proliferative culture for 5 consecutive passages. The cells at each passage were characterized for multipotent MSC properties such as proliferation kinetics, expression patterns of MSC surface markers and self-renewal associated markers, and chondrogenic differentiation. In results, the proliferation of the cells as determined by the cumulative population doubling level was observed at its peak on passage 3 and stopped after passage 5, whereas cell doubling time dramatically increased after passage 4. Expression of MSC surface markers (CD44, CD54, CD61, CD80, CD90 and Flk-1), molecule (HMGA2) and pluripotent markers (sox2, nanog) associated with self-renewal was negatively correlated with the number of passages. However, MSC surface marker (CD105) and pluripotent marker (Oct3/4) decreased with increasing the number of subpassage. cUC-MSCs at passage 1 to 5 underwent chondrogenesis under specific culture conditions, but percentage of chondrogenic differentiation decreased with increasing the number of subpassage. Collectively, the present study suggested that sequential subpassage could affect multipotent properties of cUC-MSCs and needs to be addressed before clinical applications.

• 한국작물학회지
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• 제60권1호
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• pp.107-113
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• 2015

This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by colchicine treatment. The seed germination were decreased by the higher concentration of colchicine treatment and longer soaking time. A total of 907 individuals were germinated in 16 treated plots except control (untreated plot) and 28 tetraploids were induced which was about 3.1% of the number of seed germinated. The plant regeneration rate by colchicine treatment on explant of Prunella vulgaris for. albiflora Nakai under in vitro culture was decreased with the higher concentration of colchicine. While a total of 312 individuals were regenerated in all treatments, the explant was soaked in more than 0.05% for over 1 hour, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 6 hours and 12 hours, 37 tetraploids were induced, which was about 57.8% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploid, the peak DNA content of G1 phase was 101.3 for diploid and 197.2 for tetraploid. The result confirmed the doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 19.3 in tetraploid, which was around 1.9 times as much as diploid.

• 영미문화
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• 제10권2호
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• pp.149-207
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• 2010

Written simultaneously with Prometheus Unbound, Shelley's "Julian & Maddalo" is a masterwork of dramatic poiesis, of doubling embedded in its couplets, dialogic debate on human nature and contrasted symbolic emblems. The emblems mirror each other and are themselves sites of generative paradox: the "heaven illumined" but "dreary tower" of the Maniac and the glorious sunsets on the "ever-shifting sand" of the Lido, a wasteland that is a place of self discovery but also of "abandonment" and barren mingling figured, inter alia, in its "amphibious weeds," a trope of the poem's personae. This essay also explores the poem's dramatic structure and various rhetorical devices, beginning with the Preface, a threshold of complex identity disguise that Shelley uses for veiled self-presentation, as in "Alastor," mirroring and literary references replete with nuanced ironies. I focus mainly on the complex figures of liminality Shelley uses to develop his own thoughts (as well as his ongoing debates with Byron) about man's potential for growth in thought, insight and empathy, in political reform and interpersonal and individual healing. Advancing Shelley's most optimistic ideas, Julian, escorted by Maddalo observes the Maniac, -- a living ruin whose pained eloquence reveals the link of eros to poiesis and the limits of the latter's ability to 'transform a world.' The Maniac is the core of muse-work (remembering, thinking and song) and Shelley presents him as its emblem. He also is prefigured in and reflects the quintessentially liminal Lido with its "barren embrace" of sea and land. Yet it is less the Maniac's feeling that his grief is "charactered in vain…on this unfeeling leaf" than Julian's rationales for leaving the site of pain that point to Shelley's final comment on poetry's transformative limits. As the primary haploids of the drama's meiosis re-combine and two of them, Maddalo and the maniac fall away, an analogy I briefly develop and embedded in the erotic dynamics of poiesis, Shelley suggests, as he did at the beginning of his poetic lyricism in "Alastor" and at its end in "the Triumph of Life"that images mislead and delude; that "the deep truth is imageless" and redemption is not in but beyond figuration.

• 한국결정성장학회:학술대회논문집
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• 한국결정성장학회 1996년도 The 9th KACG Technical Annual Meeting and the 3rd Korea-Japan EMGS (Electronic Materials Growth Symposium)
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• pp.293-324
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• 1996

Compact solid-state blue laser have great potential for use in optical data storage, laser beam printing, particle countering, reprographics, holography, and fluorescent bioanalysis. We report recent progresses in qualities of LiB3O5 and K3Li2(TaxNb1-x)5O15 nonlinear crystals which are essential in manufacturing bulk-type blue SHG devices. We also review newly developed violet-blue laser, 20 mW output, using intracavity frequency doubling of a diode laser pumped Cr:LiSrAlF6 laser with low loss LiB3O5 crystal as a frequency doubler.

• 한국수자원학회논문집
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• 제41권11호
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• pp.1095-1106
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• 2008

기후변화와 지구온난화현상은 지구 전체에 걸쳐 분명하게 나타나고 있으며 그에 따라 발생할 수 있는 수문 변화에 대한 연구가 다양하게 이루어지고 있다. 본 연구에서는 기후변화에 따른 유역 유출의 민감도를 평가하기 위하여 SWAT 모형을 이용하였으며 대청댐유역에 적용하였다. 모형의 보정은 1982-1995년의 월평균 하천유량을 이용하였고 1996-2005년의 자료를 이용하여 검증하였다. 기후변화에 따른 수문 변동을 정량적으로 분석하기 위하여 1988-2002년을 기준시나리오 기간으로 설정하였으며 이산화탄소 농도, 기온, 강수의 변화에 따른 총 7개의 시나리오를 구성하였다. 7개의 시나리오 중 배증 이산화탄소와 기온의 5.4℃ 증가를 반영하는 시나리오는 연평균 4～5%의 하천유량 증가를 예측하였고, 강수량의 변화를 반영하는 시나리오는 -42, -17, 17, 42%의 변화에 따라 -55, -24, 25, 64%의 비선형적인 하천유량 증감이 예측되었다. 기후인자의 변화를 복합적으로 반영하고 있는 시나리오에서는 평균 12%(봄, 여름, 가을)와 63%(겨울)의 하천유량 변화를 예측하였다. 또한 유황분석 결과 대청댐유역의 유출은 기후변화에 매우 민감한 것으로 나타났으며, 이러한 기후변화는 가뭄과 홍수의 심도와 발생기간에 큰 영향을 미칠 것으로 판단된다.

• 한국산림과학회지
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• 제87권3호
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• pp.358-365
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• 1998

장래(將來) 삼임자원(森林資源)의 지속가능(持續可能)한 이용(利用)과 지구(地球) 환경보전(環境保全)을 위한 기초(基礎) 정보(情報)를 제공(提供)하기 위해, 대기중(大氣中)의 이산화탄소 농도(濃度)의 배증(倍增)($2{\times}CO_2$)에 따른 기후변화(氣候變化)가 잠재삼림면적(潛在森林面積)에 미치는 영향(影響)을 K${\ddot{o}}$ppen의 기후(氣候)/식생구분법(植生區分法)에 근거(根據)한 식생도제작(植生圖製作) 시스템에 의해 추정(推定)했다. 식생도제작 시스템은 점(点)들의 기상자료(氣象資料)를 각각(各各)의 격자점(格子点)에 구면보간(球面補間)하며, 보간된 기상자료를 정(定)해진 식생구분법에 따라 식생형으로 변환(變換)하고, 지구(地球) 전체(全體) 및 대륙별(大陸別)의 식생도와 식생면적을 산출(産出)한다. 기상자료로서는 약 2,000지점(地點)의 측후소(測候所)로부터 관측(觀測)된 AD 1958년까지의 측정치(測定値)인 현재의 기후(氣候)와, 일본기상청(日本氣象廳)의 예측치(豫測値)인 $CO_2$배증시(培增時)의 기후를 사용하였다. 기후변화에 의해 세계의 잠재 식생대는 주로 적도지역에서 극방향으로 이동될 것이며, 잠재식생면적의 변화(變化)는 지구(地球) 육상면적(陸上面積)(150.4 억(憶)ha)의 약(約) 1/3에 해당(該當)하는 49.0억(憶)ha에 이를 것으로 예상된다. 현재의 기후와 예상(豫想)되는 기후에 대응(對應)하는 지구 전체의 식생분포형태(植生分布形態) 간(間)의 일치정도(一致程度)를 Kappa통계(統計) 분석(分析)한 결과(結果)그 값은 Good(0.63)으로 나타났으며, 기후변화에 크게 영향을 받지 않은 지역은 사막과 빙설지대로 나타난다. 세계의 잠재산림면적은 $2{\times}CO_2$ 기후하(氣候下)에서 총 68.2억ha로 추정된다. 기후변화에 의한 대륙별 잠재삼림면적에 있어서, 유럽은 거의 변화가 없을 것으로 사료되나, 북미와 아시아 대륙은 기후변화와 함께 잠재삼림면적이 증가할 것이며, 남미, 아프리카, 오세아니아에서는 잠재삼림면적이 크게 감소할 것으로 추정된다. 특히, 오세아니아에서는 기후변화에 의한 사막화 현상이 가속화될 것으로 사료된다.