• Title/Summary/Keyword: double sequence

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Operation-sequence-based Approach for Designing a U-shaped Independent-Cell System with Machine Requirement Incorporated (설비능력과 작업순서를 고려한 U-라인상에서의 셀 시스템 설계)

  • 박연기;성창섭;정병호
    • Journal of the Korean Operations Research and Management Science Society
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    • v.26 no.1
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    • pp.71-85
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    • 2001
  • This paper considers a cost model for a U-shaped manufacturing cell formation which incorporates a required number of machines and various material flows together under multi-part multi-cell environment. The model is required to satisfy both the specified operation sequence of each part and the total part demand volume, which are considered to derive material handling cost in U-shaped flow line cells. In the model several cost-incurring factors including set-up for batch change-over, processing time for operations of each part, and machine failures are also considered in association with processing load and capacity of each cell. Moreover, a heuristic for a good machine layout in each cell is newly proposed based on the material handling cost of each alternative sequence layout. These all are put together to present an efficient heuristic for the U-shaped independent-cell formation problem, numerical problems are solved to illustrate the algorithm.

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Comparative Evaluation between 1.5T vs 3.0T MRI in Brain Metastasis According to its Size

  • Jung, Woo-Seok;Jung, Tae-Sub;Heo, Jin;Lee, Jae-Hoon
    • Proceedings of the KSMRM Conference
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    • 2003.10a
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    • pp.22-22
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    • 2003
  • The purpose of this study was to compare the detection rate of brain metastasis according to size of nodule between 1.5T and 3.0T MRI 대상 및 방법: We reviewed 44 patients with primary tumors and clinical symptoms suggesting brain metastasis. After administration of double dose gadolinium-DTPA, MR imaging was performed with 3D SPGR sequence by 3.0T MRI and then with T1 SE sequence by 1.5T MRI. Consequently, comparison was done in 1.5T T1 SE sequence and 3.0T 3D SPGR sequence. With use of the signal intensity (SI) measurements in the metastatic nodules and adjacent tissue, metastatic nodule-to-adjacent tissue SI ratio were calculated. In each patient, the number of metastatic lesions detected in 1.5T and 3.0T, and their size were assessed qualitatively by three blinded readers.

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Design Optimization of Double-array Bolted Joints in Cylindrical Composite Structures

  • Kim, Myungjun;Kim, Yongha;Kim, Pyeunghwa;Park, Jungsun
    • International Journal of Aeronautical and Space Sciences
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    • v.17 no.3
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    • pp.332-340
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    • 2016
  • A design optimization is performed for the double-bolted joint in cylindrical composite structures by using a simplified analytical method. This method uses failure criteria for the major failure modes of the bolted composite joint. For the double-bolted joint with a zigzag arrangement, it is necessary to consider an interaction effect between the bolt arrays. This paper proposes another failure mode which is determined by angle and distance between two bolts in different arrays and define a failure criterion for the failure mode. The optimal design for the double-bolted joint is carried out by considering the interactive net-tension failure mode. The genetic algorithm (GA) is adopted to determine the optimized parameters; bolt spacing, edge distance, and stacking sequence of the composite laminate. A purpose of the design optimization is to maximize the burst pressure of the cylindrical structures by ensuring structural integrity. Also, a progressive failure analysis (PFA) is performed to verify the results of the optimal design for the double-bolted joint. In PFA, Hashin 3D failure criterion is used to determine the ply that would fail. A stiffness reduction model is then used to reduce the stiffness of the failed ply for the corresponding failure mode.

nif-Gene Organization and Nucleotide Sequence of nifV, nifH, D, K and nifE from Frankia Strain FaCl

  • An, Chung-Sun
    • Proceedings of the Zoological Society Korea Conference
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    • 1995.10b
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    • pp.120-120
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    • 1995
  • The total size of the pF AR1, a genomic clone of Frankia FaCI, was estimated to be about 44Kb by summation of the individual fragment length generated by single or double restriction enzymes. Southern hybridization analyses with Azotobacter vinelandii nif-genes as probes and partial sequencing analyses of the subclones revealed that organization of the nif-gene in the FaCI strain was nifV, H, D, K, E, N, X, W, B. The organization of the structural genes for nitrogenase is the same in this Frankia strain as it is in most other nitrogen-fixing prokaryotes but the positioning of the nifV-like gene relative to the nifHDK cluster differs. A consensus nif-promoter-like sequence, found at 5' of nifH, was not detected upstream of the niJV-like gene. nifV-like gene contained a ORF of 1206 NT encoding 401 amino acids. The nucleotide sequence and deduced amino acid sequence of the gene exhibit homology value of 65% and 41% with that from A vinelandii, respectively. The putative Shine-Dargamo sequences were present preceding nitK, nifH, D, K, and nifE, and in nitK gene putative start codon GTG was detected instead of A TG. The nucleotide and amino acid sequence of niIK of FaCI showed 82% and 76% homolgy with those of Frankia HFPCc 13, respectively. Amino acid sequence of niIK showed 69% and 61% homology with those of A vinelandii, Klebsiella pnewnoniae, respectively, while that of nifE 73% and 71%, respecti vely.i vely.

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Genetic Identification of Hybrids between Rhodeus uyekii and R. notatus by Sequence Analysis of RAG-1 Gene (RAG-1 유전자의 염기서열 분석에 의한 각시붕어 Rhodeus uyekii와 떡납줄갱이 R. notatus 잡종의 동정)

  • Yun, Young-Eun;Lee, Il-Ro;Park, Sang-Yong;Kang, Eon-Jong;Kim, Eung-O;Yang, Sang-Keun;Nam, Yoon-Kwon;Bang, In-Chul
    • Journal of Aquaculture
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    • v.22 no.1
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    • pp.79-82
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    • 2009
  • Reciprocal interspecific hybrids between two bitterling species Rhodeus uyekii (RU) and R. notatus (RN) were genetically identified based on the partial sequence analysis of recombination activating gene-1 (RAG-1) gene. Out of 863 bp positions analyzed, 13 nucleotide substitutions were detected between the two parental species (RU and RN genotypes). Both the induced hybrids (RU female$\times$RN male; UN genotype) and their reciprocal counterparts (RN female$\times$RU male; NU genotype) displayed the double peaks (or polymorphism) of sequence chromatograms at the 13 diagnostic positions, indicating that those hybrids were actual karyogamy derived from the two parental haploid genomes. However, it was not possible to distinguish between the reciprocal interspecific hybrids.

Clinical validation of the 3-dimensional double-echo steady-state with water excitation sequence of MR neurography for preoperative facial and lingual nerve identification

  • Kwon, Dohyun;Lee, Chena;Chae, YeonSu;Kwon, Ik Jae;Kim, Soung Min;Lee, Jong-Ho
    • Imaging Science in Dentistry
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    • v.52 no.3
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    • pp.259-266
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    • 2022
  • Purpose: This study aimed to evaluate the clinical usefulness of magnetic resonance (MR) neurography using the 3-dimensional double-echo steady-state with water excitation (3D-DESS-WE) sequence for the preoperative delineation of the facial and lingual nerves. Materials and Methods: Patients underwent MR neurography for a tumor in the parotid gland area or lingual neuropathy from January 2020 to December 2021 were reviewed. Preoperative MR neurography using the 3D-DESS-WE sequence was evaluated. The visibility of the facial nerve and lingual nerve was scored on a 5-point scale, with poor visibility as 1 point and excellent as 5 points. The facial nerve course relative to the tumor was identified as superficial, deep, or encased. This was compared to the actual nerve course identified during surgery. The operative findings in lingual nerve surgery were also described. Results: Ten patients with parotid tumors and 3 patients with lingual neuropathy were included. Among 10 parotid tumor patients, 8 were diagnosed with benign tumors and 2 with malignant tumors. The median facial nerve visibility score was 4.5 points. The distribution of scores was as follows: 5 points in 5 cases, 4 points in 1 case, 3 points in 2 cases, and 2 points in 2 cases. The lingual nerve continuity score in the affected area was lower than in the unaffected area in all 3 patients. The average visibility score of the lingual nerve was 2.67 on the affected side and 4 on the unaffected side. Conclusion: This study confirmed that the preoperative localization of the facial and lingual nerves using MR neurography with the 3D-DESS-WE sequence was feasible and contributed to surgical planning for the parotid area and lingual nerve.

Tensile load bearing capacities of co-cured single and double lap joints (외면 및 양면겹치기 동시경화조인트의 인장하중 전달용량에 관한 연구)

  • 신금철;이정주
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2001.10a
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    • pp.95-98
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    • 2001
  • Co-cured joining method is an efficient joining technique because both curing and bonding processes for the composite structures can be achieved simultaneously. It requires neither an adhesive nor a surface treatment of the composite adherend because the excess resin, which is extracted from composite materials during consolidation, accomplishes the co-cured joining process. In this paper, we considered three bond parameters, affecting tensile load bearing capacity of the co-cured single and double lap joints. Filially, we nave presented optimal bonding conditions for co-cured single and double lap joints with steel and composite adherends under tensile loads.

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Anti-DNA Autoantibodies from on MRL/Ipr Mouse

  • Park, Jeong-Soo;Kim, Young-Tai;Lee, Chan-Hee;Youn, Jung-Koo;Jang, Young-Ju
    • Animal cells and systems
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    • v.2 no.3
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    • pp.371-375
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    • 1998
  • Twenty-one monoclonal anti-DNA autoantilndies were produced by fusing spleen cells from an autoimmune MRL/lpr mouse with SP2/0 myeloma cells. Hybridomas generated by the fusions were chosen for cloning on the basis of DNA binding by supernatant antibody. Each monoclonal antibody was purified to homogeneity and analyzed for the heavy and light chain isotypes and the binding specificity for single-stranded DNA, double-stranded DNA, and RNA. Sequence specificities and isoelectric points of the antibodies were also examined. All of the antibodies were lgG and tended to bind to both single-stranded and double-stranded DNA with a preference for the double-stranded form. Some of them also bound to RNA. Isoelectric points of the antibodies were shown to be high. The antibodies described in this report have characteristics of pathogenic anti-DNA antibodies.

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Cloning, Expression, and Characterization of DNA Polymerase from Hyperthermophilic Bacterium Aquifex pyrophilus

  • Choi, Jeong-Jin;Kwon, Suk-Tae
    • Journal of Microbiology and Biotechnology
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    • v.14 no.5
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    • pp.1022-1030
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    • 2004
  • The gene encoding Aquifex pyrophilus (Apy) DNA polymerase was cloned and sequenced. The Apy DNA polymerase gene consists of 1,725 bp coding for a protein with 574 amino acid residues. The deduced amino acid sequence of Apy DNA. polymerase showed a high sequence homology to Escherichia coli DNA polymerase I-like DNA polymerases. It was deduced by amino acid sequence alignment that Apy DNA polymerase, like the Klenow fragment, has only the two domains, the $3'{\rightarrow}5'$ exonuclease domain and the $5'{\rightarrow}3'$ polymerase domain, containing the characteristic motifs. The Apy DNA polymerase gene was expressed under the control of T7lac promoter on the expression vector pET-22b(+) in E. coli. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $UNO^{TM}$ Q column chromatographies. The optimum pH of the purified enzyme was 7.5, and the optimal concentrations of KCl and $Mg^{2+}$ were 20 mM and 3 mM, respectively. Apy DNA polymerase contained a double strand-dependent $3'{\rightarrow}5'$ proofreading exonuclease activity, but lacked any detectable $5'{\rightarrow}3'$ exonuclease activity, which is consistent with its amino acid sequence. The somewhat lower thermostability of Apy DNA polymerase than the growth temperature of A. pyrophilus was analyzed by the comparison of amino acid composition and pressure effect.