• 식물병연구
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• 제29권3호
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• pp.286-294
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• 2023

Biological and molecular characterization of a Korean isolate of Orthotospovirus chrysanthinecrocaulis (formerly known as chrysanthemum stem necrosis virus, CSNV) isolated from Chrysanthemum morifolium was determined using host range and sequence analysis in this study. Twenty-three species of indicator plants inoculated mechanically CSNV-Kr was investigated for determination of host range. CSNV-Kr induced various local and systemic symptoms in the inoculated plant species. CSNV-Kr could not infect three plant species and induced symptomless in systemic leaves in Nicotiana tabacum cultivars, though the plant samples reacted positively with the antiserum to CSNV by double-antibody sandwich-enzyme-linked immunosorbent assay. The complete genome sequence of CSNV-Kr was determined. The L RNA of CSNV-Kr consists of 8,959 nucleotides (nt) and encodes a putative RNA-dependent RNA polymerase. The M RNA of CSNV-Kr consists of 4,835 nt and encodes the movement protein (NSm) and the glycoprotein precursor (Gn/Gc protein). The S RNA of CNSV-Kr consists of 2,836 nt and encodes NSs protein and N protein. The Gn/Gc and N sequence of CSNV-Kr were compared with those of previously published CSNV isolates originating from different countries at nucleotide and amino acid levels. The Gn/GC sequence of CSNV-Kr shared 98.8-99.5% identity with CSNV isolated from other countries and the N sequence of CSNV-Kr shared 98.8-99.6% identity. No particular region of variability could be found in either grouping of viruses. All of the CSNV isolates did not show any relationship according to geographical origins and isolation hosts, suggesting no distinct segregation of the CSNV isolates.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.61-64
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• 2001

Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double $\delta$ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single $\delta$ system.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.2004-2007
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• 2006

An efficient method for the in vitro reassembly of homologous DNA sequences is presented. The proposed method involves obtaining single strands of homologous genes and hybridizing them to obtain partially hybridized heteroduplex DNA; cleaving the single-stranded regions of the heteroduplex DNA using S1 nuclease to generate double-strand DNA fragments; denaturing the double-strand DNA fragments to generate single-strand DNA fragments; conducting a series of polymerase chain reactions (PCR) using the single-strand DNA fragments as internal primers and a mixture of homologous DNA as templates to obtain elongated reassembled DNA; and finally, amplifying the reassembled DNA by a PCR using terminal primers. As a result, DNA reassembly could be achieved between homologous genes with a sequence similarity as low as 78%.

• 한국통신학회논문지
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• 제29권5C호
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• pp.692-699
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• 2004

본 논문에서는 변경된 Given회전을 이용한 QR 분해와 이를 기반으로 하는 RLS 알고리듬의 구현에 대하여 연구하였다. Givens 회전은 CORDIC(Coordinate Rotation Digital Computer) 연산을 반복 수행하여 구한다. QR 분해의 계산 시간을 줄이기 위해 1회의 Givens 회전에 필요한 CORDIC 연산의 반복 회수를 제한하였으며 보정계수를 계산할 때의 제곱근 계산을 없애기 위해 2회전 방법을 사용하였다.

• International journal of advanced smart convergence
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• 제1권1호
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• pp.27-33
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• 2012

In this paper, we analyze and simulate performance of space time block coded (STBC) binary pulse amplitude modulation-direct sequence (BPAM-DS) ultra-wideband (UWB) systems with double binary turbo code in indoor environments for various ubiquitous healthcare (u-healthcare) applications. Indoor wireless channel is modeled as a modified Saleh and Valenzuela (SV) model proposed as a UWB indoor channel model by the IEEE 802.15.SG3a in July 2003. In the STBC encoding process, an Alamouti algorithm for real-valued signals is employed because UWB signals have the type of real signal constellation. It is assumed that the transmitter has knowledge about channel state information. From simulation results, it is shown that the STBC scheme does not have an influence on improving bit error probability performance of the BPAM-DS UWB systems. It is also confirmed that the results of this paper can be applicable for u-healthcare applications.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1999년도 추계학술대회 논문집 학회본부 A
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• pp.255-257
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• 1999

Fault location algorithms based on the current distribution factors under the one-phase to earth fault condition of a double-circuit line are presented. The derivation method for current distribution factors is showed, to calculate fault current, fault resistance and the zero sequence current of other parallel circuit which are unknown. As the proposed algorithms 1,2,3 embodies an accurate location by the voltage and the current of the relaying point.

• 한국통신학회논문지
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• 제31권7A호
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• pp.696-701
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• 2006

In this paper, we analyze the performance of double-dwell hybrid initial acquisition in DS-UWB system via detection, miss, false alarm probabilities and mean acquisition time. In the analysis, we consider the effect of the acquisition sequence, and deployment scenario of the abundant multipath components over the small coverage of the piconet in DS-UWB system. Based on the simulation, we obtain various performance on the mean acquisition time by varying the parameters, such as the total number of hypotheses to be searched, subgroup size, and dwell time. Then, we suggest the optimum parameter set for the initial acquisition in DS-UWB system.

• Bulletin of the Korean Chemical Society
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• 제12권2호
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• pp.219-224
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• 1991

New binuclear Ni(Ⅱ) and Cu(Ⅱ) complexes with various alkyl derivatives of 1,2-bis(1,3,6,8,10,13-hexaaza-1-cyclotetradecyl) ethane, in which two fully saturated 14-membered hexaaza macrocyclic subunits are linked together by an ethylene chain, have been synthesized by the one step template condensations of formaldehyde with ethylenediamine and appropriate primary alkyl amines in the presence of the metal ions. Each macrocyclic subunit of the double-ring macrocyclic complexes contains one alkyl pendant arm and has a square planar geometry with a 5-6-5-6 chelate ring sequence. The visible spectra and oxidation properties indicate that the metal-metal interaction of the binuclear complexes are not significant. Synthesis, characterization, and the properties of the complexes are presented.

• Journal of Photoscience
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• 제9권2호
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• pp.488-490
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• 2002

DNA photodamage mediated by photosensitizers are believed to play an important role in solar UVA carcinogenesis. We investigated the relationship between the DNA-damaging abilities of photoexcited xanthone analogues (as photosensitizers) and their highest occupied molecular orbital (HOMO) energies. DNA damage was examined using /sup 32/P-labeled DNA fragments obtained from the p53 tumor suppressor gene. These compounds induced DNA photodamage in a similar manner, and the extents of DNA damage were following order: xanthone> thioxanthone > acridone. Photoexcited xanthone caused nucleobase oxidation specifically at 5'-G of GG sequence in double-stranded DNA. An oxidative product of 2'-deoxyguanosine, 8-hydroxy-2'-deoxyguanosine (8-OHdG), was detected, and the amount was decreased by DNA denaturation. These findings suggest that photoexcited xanthone generates 8-OHdG at 5'-G of GG in double-stranded DNA through electron transfer. The calculated HOMO energies of these photosensitizers decreased in the following order: xanthone> thioxanthone > acridone. This study has demonstrated that DNA-damaging abilities of these photosensitizers increased exponentially with an increase in their HOMO energies.

• 한국광학회지
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• 제24권1호
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• pp.9-16
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• 2013

본 논문에서는 근축광학적인 대수식(algegraic equations)과 설계경험적인 근사조건을 통해 텔레센트릭 렌즈를 구조적으로 이해할 수 있는 일반화된 관계식을 찾는다. 이 관계식은 $f{\theta}$ 공식으로 명명되고, 단매렌즈, 이중가우스렌즈, 쿠크(Cooke)의 삼중렌즈, 쿠크의 삼중렌즈와 이중가우스렌즈가 결합된 복합렌즈의 순서로 단계적으로 적용된다. 그리고 이 공식은 조명광학용으로 사용되는 단매의 플라이아이 렌즈와 집광렌즈, 2매의 플라이아이 렌즈와 집광렌즈에 대해서도 순차적으로 적용된다. 여기서, 집광렌즈는 텔레센트릭 렌즈와 광학적으로 동일한 속성을 가진다. 결론적으로, 본 논문에서 찾은 $f{\theta}$ 공식은 텔레센트릭 렌즈에 대한 구조적인 이해를 이끌어냄과 동시에 직관적인 방식의 설계에도 활용될 수 있음을 보여 준다.