• Title/Summary/Keyword: double exposure

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Vapor Exposure Effect of a Casting Solution on the Embedding and Radioactive Detection of CAYS in Double-layered Polysulffne Film (방사능탐지용 CAYS 함침 이중구조 폴리설폰막의 형상 및 특성에 제막공정의 습도가 미치는 영향)

  • Han Myeong-Jin;Nam Suk-Tae;Lee Kune-Woo;Seo Bum-Kyoung
    • Membrane Journal
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    • v.15 no.3
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    • pp.198-205
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    • 2005
  • Double-layered polymer films to assay the radioactive contamination were formulated using polysulfone (PSF) and cerium activated yttrium silicate (CAYS), consisting of a dense support layer and a CAYS-holding top layer prepared via the diffusion-induced phase inversion. As the vapor exposure process was omitted, the CAYS-holding layer showed a typical asymmetric structure, with CAYS being transfixed into the polymer network spread with large macropores. With the increase in vapor exposure time before immersion, morphology of the films transformed from asymmetric to sponge-like structures, with CAYS being localized in cellular structure. The border structure between the two layers reflects the phase inversion behavior of a cast solution during the coagulation. In the radioactive detection, the polymer phase in a film holding a sponge-like structure is so dense that the radionuclides, deposited on the film, could not filter through the phase, consequently resulting in the loss in the detection efficiency of the film.

Mechanism of guanine-specific DNA damage by UVA and its role in photocarcinogenesis and photoaging

  • Kawanishi, Shosuke;Oikawa, Shinji;Hiraku, Yusuke
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.150-153
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    • 2002
  • Solar UV light is a well-known carcinogen. UVA radiation is probably carcinogenic to humans. In addition, recent investigations point to the importance of UVA irradiation in the photoaging. We investigated the mechanism of sequence- specific DNA damage using $\^$32/P-Iabeled DNA fragments in relation to carcinogenesis and aging. Furthermore, we investigated whether UVA accelerates the telomere shortening in human WI-38 fibroblasts. The exposure of double- stranded DNA fragments to 365 nm light in the presence of endogenous sensitizers produced sequence-specific cleavage at the 5' site of 5'-GG-3' and 5'-GGG-3' sequences. In addition, HPLC analysis revealed that sensitizers plus 365 nm light increased the 8-oxodG content of double-stranded DNA. We discuss the mechanisms of guanine-specific DNA damagecaused by excited photosensitizers in relation to carcinogenesis and aging.

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Analysis of Three-Dimensional Natural Convection Using a Holographic Interferometric Tomography (홀로그래피 간섭 토모그래피를 이용한 3 차원 자연대류 해석)

  • Shim, Dong-Sik;Lee, Soo-Man;Kang, Bo-Seon;Cha, Dong-Jin;Joo, Won-Jong
    • Proceedings of the KSME Conference
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    • 2001.06d
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    • pp.497-502
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    • 2001
  • Three-dimensional natural convection from a discrete flush-mounted circular heat source on the bottom of a cubic enclosure was studied by using a holographic interferometric tomography. The heat source was located at the off-center of the bottom plate so that three-dimensional temperature field can be achieved. A set of multidirectional holographic interferogram was recorded by employing a double-reference beam, double-exposure holographic technique in order to eventually reconstruct the temperature fields. The recorded interferometric data appear good enough to be further processed to extract optical pathlength data from them and finally reconstruct the temperature fields. A complete analysis of the temperature fields including the field reconstructions and comparison with thermocouple measurements is underway and will be reported shortly.

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Development of a Holographic Interferometric Tomography System and Its Application to Three-Dimensional Natural Convection (홀로그래피 간섭 토모그래피 개발 및 3차원 자연대류 열전달에의 적용)

  • Lee, Soo-Man;Kang, Min-Gu;Cha, Dong-Jin;Joo, Won-Jong;Kang, Bo-Seon
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.1609-1614
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    • 2003
  • In this study, a practical holographic interferometric tomography system, which is instantaneous and non-contact for measuring three dimensional flow field, was developed. The system consists of holographic recording/reconstruction system, fringe analysis code and computational tomography code and it is developed with Gill environment for general users. The developed system was applied to three-dimensional natural convection from a discrete flush-mounted circular heat source on the bottom of a cubic enclosure. The heat source was located at the off-center of the bottom plate so that three-dimensional temperature field can be achieved. A set of multi-directional holographic interferograms was recorded by employing a double-reference beam, double-exposure holographic technique. Optical pathlength data were extracted from the recorded interferometric data and finally three dimensional temperature field inside the cube was reconstructed.

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Electron Spin Resonance and Electron Nuclear Double Resonance Studies on the Photoinduced Charge Separation of N-Methylphenothiazine in Phenyltriethoxysilane, Vinyltriethoxysilane and Methyltriethoxysilane Gel Matrices

  • Kang, Young-Soo;Park, Chan-Young
    • Journal of the Korean Magnetic Resonance Society
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    • v.4 no.2
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    • pp.91-102
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    • 2000
  • The photoproduced cation radical of N-methylphenothiazine doped in the different kind of matrices of phenyltriethoxysilane (PhiTEOS), vinyltriethoxysilane (VTEOS), and methyloiethoxysilane (METOS) was comparatively studied with electron spin resonance (ESR) and electron nuclear double resonance (ENDOR). The photoinduced charge separation efficiency was determined by integration of ESR spectra which correspond to the amount of photoproduced cation radical in the matrices. This was correlatively studied with the polarity and pore size of the gel matrices. The polarity of the matrices was comparatively determined by measuring λ$\sub$max/ values of PC$_1$ in the different matrices. The relative pore size among the matrices was determined by measuring relative proton matrix ENDOR line widths of the photoproduced cation radical of PCI. The decay kinetic constants of the cation radical of PCI in the different matrices was relatively studied with fitting the biexponential decay curves after exposure into the ambient condition. This is correlatively interpreted with the polarity and pore size of the matrices.

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The Properties of Temperature History of Concrete with Surface Insulating Material in Cold Weather Concreting (한중콘크리트 시공시 표면 단열재 변화에 따른 콘크리트의 온도이력 특성)

  • 문학용;신동안;김경민;김기철;오선교;한천구
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2003.05a
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    • pp.7-11
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    • 2003
  • This study investigate the hydration heat history with variation of surface insulating material in cold weather concreting. According to the results, the temperature of concrete lowers below zero in 24hours, so early frost damage occurs in the case of exposure and 1 fold bubble sheet, but the lowest temperature keeps above zero, so a adiabatic effect is very favorable in the case of double bubble sheet and 부직포. Compressive strength of core specimen at 7 and 28 days is highest In the case of double bubble sheet and 부직포. But, considering convenience of construction and economical efficiency, it is thought that the most effective surface insulating material is 1 fold bubble sheet +blanket.

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Double Labeling of Binding Sites in Cellulosic Substrates Using Endo- and Exoglucanase-Gold Complexes

  • Bae Hyeun-Jong
    • Plant Resources
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    • v.8 no.3
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    • pp.175-180
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    • 2005
  • Thin sections of cellulose fibers were incubated with an endo- and an exoglucanase labeled with gold particles of differing sizes. The hydrolytic sites were then visualized under transmission electron microscopy (TEM). The potential interaction between the ${\beta}$-1, 4-glucan substrates and the endo- and the exoglucanases was investigated using cellulosic and lignocellulosic substrates. The simultaneous visualization was very successful in distinguishing preferred substrates for each cellulase in lignocellulosic substrates. When plant lignocellulose was preincubated with endocellulase, density of the gold labeling greatly increased suggesting that preliminary exposure of lignocellulosic material to endocellulase may have enhanced the accessibility of the substrate to endocellulase and exocellulase. This result provided a plausible explanation for the observed endo/exo cellulase co-hydrolysis.

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Dry-etch Characteristics of InP/InGaAsP Photonic Crystal Structure (InP/InGaAsP 광자결정 구조 제작을 위한 건식 식각 특성)

  • Lee, Ji-Myon
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.12
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    • pp.1271-1276
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    • 2004
  • Two-dimensionally arrayed nanocolumn lattices were fabricated by using double-exposure laser holographic method. The hexagonal lattice was formed by rotating the sample with 60 degree while the square lattice by 90 degree before the second laser-exposure. The reactive ion etching for a typical time of 30 min using CH$_4$/H$_2$ plasma enhanced the aspect-ratio by more than 1.5 with a slight increase of the bottom width of columns. The etch-damage was observed by photoluminescence (PL) spectroscopy which was removed by the wet chemical etching using HBr/$H_2O$$_2$/$H_2O$ solution, leading into the enhanced PL intensities of the PCs.

Temperature History of Concrete Corresponding to Various Bubble Sheets Layer and Curing Temperature (양생온도 변화 및 버블시트 두께변화에 따른 콘크리트의 온도이력특성)

  • Hong, Seak-Min;Baek, Dae-Hyun;Han, Min-Cheol;Han, Cheon-Goo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2008.11a
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    • pp.21-25
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    • 2008
  • In this paper, the temperature history and the strength development of concrete corresponded to various bubble sheets layer and curing temperature. Based on the results, In case of the test temperature of -5℃, concrete subject in the exposure condition, result in a frost damage at initial stage by a fall of below zero temperature. In case of the combination of PE film and non woven fabric was after 36 hour, and combination of bubble sheet over double, a tremendous insulating effect of bubble sheet over double is confirmed due to the temperature of concrete fall of below zero temperature after 60 hours. Meanwhile, regarding the -15℃ of temperature, special measure for insulation curing is necessary to secure stability against early frost damage because frost damage was not affected by the lapping thickness of bubble sheet subjected to severe cold weather condition.

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Metal Effects of Urban Air Particulates on Cytokine Production and DNA Damage

  • Lee, Kwan-Hee;Hong, Yun-Chul
    • Toxicological Research
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    • v.17 no.4
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    • pp.255-265
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    • 2001
  • Epidemiologic studies have demonstrated an association between short-term exposure to particulate air pollutants and increased mortality. However the biological mechanism underlying these associations have not been fully established and also the chemical and physical characteristics of the pollutant particles are not well understood. The metal constituents of air pollutant particles and their bioavailability are considered to Play an important role as possible mediators of Particle-induced airway injury and inflammation. Sprague-Dawley rat alveolar macrophage cells (NR8383) were exposed to airborne and acid-leached particulate matter (PM). Titanium oxide and nickel subsulfide were used as negative and positive controls. Particle-induced reactive oxygen species formation in cells was detected using the fluorescent probe 2',7'-dichlorofluorescin diacetate. Expression of TNF-$\alpha$ and IL-6 were measured by enzyme-linked immunosorbent assay, and PM-induced DNA double-strand breaks were determined with $\lambda$DNA/Hind III marker. Metals associated with air pollutant particles mediated intracellular oxidant production in alveolar macrophages, and the cytotoxicity and proinflammatory cytokine production induced by PM were associated with oxidative stress. The oxidants produced by air pollutant particles also are likely to induce DNA double-strand breaks. Our findings in alveolar macrophage cells exposed to PM and acid-leached PM support the hypothesis that metal components in urban air pollutants and their bioavailabilities might play an Important role in the induction of the adverse health effects.

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