• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.87-95
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• 2008

Probiotics are live organisms that when administered in adequate amounts confer a health benefit on hosts. The administration of direct-fed microbials (DFM) such as lactobacilli and bacillus, may be a more direct approach to beneficially alter gastrointestinal microflora than altering dietary ingredients or supplementing with growth-promoting levels of antibiotics. It is apparent that microbes have an important influence on immune development and resistance to infection; that microbes are not static colonizers of our bodies, but are dynamic, symbiotic coresidents. And it can improve the surrounding environments; decrease the malodor caused by degrade the excrement. Recently, new paradigm such as environment protection and safe food have been settled. In domestic farm house, there is a great demand for probiotics as a substitute of antibiotics for the improvement of environmental quality and the production of a competitive goods. Probiotics circulated in a country have three categories: an animal medicine permitted by national veterinary research quarantine service (NVRQS), a support feed registered in city or country house, and not-registered goods. However, lots of unqualified goods were produced and circulated. And thus, it is in urgent need of evaluating the present situation and effect of probiotics. This study was conducted to evaluate the system of a probiotics as a livestock-environment improving agents for the alternation of antibiotics and quality control of it.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.4
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• pp.603-610
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• 2018

The development of industry and the increase in the use of fossil fuels have accelerated the process of global warming and climate change, resulting in more frequent and intense natural disasters than ever before. Since electricity facilities are often installed outdoors, they are heavily influenced by natural disasters and the number of related accidents is increasing. In this paper, we analyzed the statistical status of domestic electrical fires, electric shock accidents, and electrical equipment accidents and hence analyzed the risk associated with climate change. Through the analysis of the electrical accidental data in connection with the various regional (metropolitan) climatic conditions (temperature, humidity), the risk rating and charts for each region and each equipment were produced. Based on this analysis, a basic electric risk prediction model is presented and a method of displaying an electric hazard prediction map for each region and each type of electric facilities through a website or smart phone app was developed using the proposed analysis data. In addition, efforts should be made to increase the durability of the electrical equipment and improve the resistance standards to prevent future disasters.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.2
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• pp.217-225
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• 2010

Most of the steel bridges in Korea are being currently designed by the allowable stress design method that uses the conventional deterministic factors of safety. However the limit state design based on the concept of probability, statistics and reliability engineering is becoming very popular as a global standard deign method, leading the rational and economic bridge design. As part of the fundamental research to establish the load and resistance factor design(LRFD) of steel bridges considering domestic environmental conditions and regional characteristics, an experimental design is conducted by applying AASHTO-LRFD specification especially to a steel closed-box girder, which occupies relatively a large portion of steel bridges in Korea. Throughout the experimental design according to various sectional changes, some of the issues to be considered in the LRFD design of a composite steel closed-box girder bridge are examined. In this process, an Excel-based design verification program is developed for easy computation and prevention of errors. Quantitative reliability levels of the bridge sections designed by LRFD are also estimated using a reliability analysis method, and compared with the target reliability indexes applied in the LRFD design to verify the validity of the procedure and methodology used in this study.

• Research in Community and Public Health Nursing
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• v.9 no.1
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• pp.40-63
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• 1998

A long life is the desire of many people. The purpose of this study was to describe long life factors for people aged one hundred and above on Chju island. The research was conducted using a phenomenological method to add understanding to this subject. The field work for this study was done from July, 1997 to February, 1998 on Cheju island. The sample consisted of 10 persons of one hundred or above and 12 families. They were audio-recorded and analyzed using Colaizzi's (1978) method. The results of the present study shows the following: The thoughts of the persons one hundred and above showed ten important concepts; 'dietary regimen', 'mental regimen', 'sleep regimen', 'residence regimen', 'health regimen', 'labor regimen', 'exercise regimen', 'being born', 'filial piety and posterity', 'attachment to life'. Concerning 'Dietary Regimen': Dietary regulation, Preferential food intake, Living on vegetables, Light eating, and concern for longevity in food intake were important. Concerning 'Mental Regimen' : Harmony, Clearheadedness, Mildness, Abstinence, Generosity, Relaxation, Gaiety, Inspiration and Strength were important. Concerning 'Sleep Regimen'; Enough sleeping hours, Sound sleep, Curved sleeping posture, and Comfortable bedding were important. Concerning 'Residence Regimen' ; Clean dwelling, Pleasant surroundings, Thatched house life, Living in the village with good-hearted people, and Regular life were important. Concerning 'Health Regimen'; Temperance of drug, use Proper temperature, Proper humidity, and a Clean body were important. Concerning 'Labor Regimen'; Agricultural labor, Diligence, and Domestic labor were important. Concerning Exercise Regimen': Proper exercise, and Sunbathing were important. Concerning 'Being Born' ; Strong physical constitution, Longevity lineage, and Destiny were important. Concerning 'Filial Piety and Posterity' : Showing respect to a long-lived elder and Reducing anxiety were important. Concerning 'Attachment to Life' ; The desire to live long was important. The following conclusion was made from the above results. Human beings cannot avoid death, but the span of life can be prolonged to the maximum span of human life by wisdom, health care, and proper environmental conditions. As a result, a health regimen must be imposed for longevity such as controling food intake, having positive thoughts, being relaxed, sleeping enough, clean dwelling, taking care of oneself which can improve immunity and resistance to decrease, as well as proper labor and exercise. In addition, when filial piety is served, the desire of longevity and retaining one's youthfulness for a long time can be achieved. These should be done to live long and lead a qualitative life. By the results of this research, the following is suggesed for nursing. To satisfy the basic nursing principle that nursing must be practiced to help people live long, education must be spread to people who are nursed and their family so that they can lead a healthy life. And in nursing elders, nurses must recognize that elders have a strong desire for life, even though they may have negative thoughts about life at times. Therefore nurses have to respect the elders' value of life, and help them improve their self-esteem and self -fulfilment.

• Journal of the Korean Recycled Construction Resources Institute
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• v.1 no.3
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• pp.189-196
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• 2013

In order for efficient recycling and eco-friendly treatment of construction waste, there is a need to apply a building separation and dismantling technique early on in the stage of waste generation. This study was conducted to analyze the relevant domestic circumstances and propose the applicable range of buildings in order to introduce a building separation and dismantling system to Korea. For this purpose, related policies and systems implemented overseas and the current situation of buildings were examined, and the workability of separation and dismantling of buildings according to their uses was compared. Also, the economic impact of the separation and dismantling system was examined and a survey was conducted, seeking the opinions of the relevant companies regarding the need for the system and the selection criteria for eligible buildings. Based on the results, it was determined that it would be impossible to apply the separation and dismantling system to all buildings in Korea, considering the current technical power and economic situation, and that the system should be introduced in phases, according to the building classification determined based on floor area and use, as the cost may vary depending on a number of variables including the level of difficulty and field conditions.

• Journal of the Korean Society of Costume
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• v.56 no.3 s.102
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• pp.1-11
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• 2006

Che Guevara spearheading the Cuban Revolution was not only the icon as the revolutionary to the New-leftists of the 1960s advocating the ideology of Marxism but, as the cultural revolutionary, had a tremendous influence on the younger generation living in the digital age Che Guevara take on a figure of both the symbol of the Leftist but the romantic revolutionary because he had the external features such as black beret, red stars, military upper jackets and trousers, beard and pipe tobacco. In fact, the symbolic image of Che Guevara was made as the popular image by the avantgarde artists and political vanguard forces of the times under the necessity of Cuban government. Afterwards, the image of Che Guevara has been patronized in making people of aware of the resistant and revolutionary image to capital, power and the power of the media and symbolized as the resistant image to the American capital as well as the revolutionary guerrilla. And his image has continued to be reproduced and symbolized for the commercial and political purposes and as the enthusiastic image of youth culture. This can be seen as having been created as a new image by the popular culture formed by the development of the cyber culture and mass media in the cyberspace shaped by contemporary 'N' generations. The use of Che Guevara's symbolic image was made in the fashion field as well as in the cultural and artistic circles. The borrowing of the icon of Che Guevara represented in a fashion field is attributed to his free spirit, and it can be seen that fashion exists as the vehicle for representing both the symbol system and the sign system containing ideologies and texts as the communicator of resistance to the regime and to social issues. Therefore, this study attempted to investigate the commercial iconization of Che Guevara in the 1990s by comparing the ideology of the symbol in the 1960s and the 1990s and inquire into the borrowing of his image by the fashion domain as well as the fashion worn by him by reference to domestic books and materials on the fashion site. Thereby, this study attempted to make clear that the borrowing of Che Guevara in the realm of fashion since the 1990s not only contained the meaningful interpretation as the symbolic code in the culture of young people living in a digital era but fashion performs an intervening role in the cultural phenomenon.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.55-59
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• 2007

The study was carried out to develop transformants resisting to Phyophthora blight disease in the domestic pepper cultivar Subicho. In transforming of syn600 promoter with elicitin gene using Agrobacterium (LBA4404/pBI101 syn600-syn${\alpha}$-elicitin) to cotyledons of pepper, rate of shoot formation in 'Subicho' was 11.1% in medium containing 3 mg/L zeatin and 0.05 mg/L NAA, and also 12.8% in medium containing combination of 4 mg/L zeatin and 0.05 mg/L MAA. For PCR reaction using elicitin gene primer of transformants regenerated from cotyledons, we detected a specific band of 536 bp, and also showed strong signal at position of 536 bp in accordance with NPTII gene used as probe in Southern blot. Transformants pepper shown resistance to blight fungus was inoculated to seedlings of the $T_{1}\;and\;T_{2}$ transformants by concentration (density: zoo spore $10^{3}/mL$).

• Korean Security Journal
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• no.31
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• pp.125-155
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• 2012

Terrorist organization has shown the trend of secret organization and it is harder to cope with terrorism because of uncertainty of terrorism. Homegrown terrorism is the one, whose preparation, execution and effect are restricted to domestic area. By the way, in the worldwide economic depression, violence and radical demonstration have shown the expansion trends as in Middle East, political revolution of Africa, anti-social resistance of Europe and Wall Street Occupation of USA. Homegrown terrorism is occurring in various countries such as UK and Spain as well as USA. Specialists warn homegrown terrorism in Korea. The purpose of this study was to prospect the possibility of homegrown terrorism that can be generated in the transfer to multi-culture society as various foreigners come to Korea rapidly and suggest the method to cope with the trend. The study analyzed environment and analysis of homegrown terrorism that Korea faces now. The methods to cope with homegrown terrorism are as follows. First, distribution of radical homegrown terrorism via internet should be prevented. Second, the connection between terrorist organization and homegrown terrorist should be prevented. Third, there should be a cooperation among government, residents and religious group. Fourth, there should be an open approach against multi-culture society. Fifth, there should be a systematic control for cause of new conflict. Finally, there should be a long-term approach to cause of new conflict. If we do not make an effort to prevent homegrown terrorism, terrorism environment may face new aspect and national and social cost for it will increase.

• Journal of the Korean GEO-environmental Society
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• v.10 no.5
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• pp.19-26
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• 2009

Rammed Aggregate Pier (RAP) method is intermediate foundation between deep and shallow foundation, and it has been built in world wide. RAP represents a relatively new method that has grown steadily over 19 years since Geopier of USA developed this revolutionary method in 1989. The investigation and research in domestic is not accomplished. In this paper, the examined details of different spacing of piles, bearing capacities, respectively, conclude with recommendations on how RAP can be used in future needs. This documentation further provides comparisons of the laboratory test results which were obtained from changing the spacing of piles, namely installed rammed aggregate pier. Laboratory model test was administered in a sand box. Strain control test was conducted to determine the bearing capacities of the piers; 20 mm, 30 mm and 40 mm RAP in diameter using drilling equipment to make holes were installed in sand at initial relative densities of 40%. By comparing different spacing of piles, in this experiment, piles are spaced structually span, form a ring shape, narrowing the distance of each other, to the center. the result shows that as diameter of pier is bigger in diameter, bearing capacity also dramatically increased due to raised stiffness. Also, as the space between each piers was closed, the settlement rate of soil was decreased significantly. From the test results, as the space between each piles were getting closer, it allows greater chances to have more resistance to deformation, and shows more improved stability of structures. After from the verification work which is continuous leads the accumulation of the site measuring data which is various, and bearing capacity and the settlement is a plan where the research will be advanced for optimum installed RAP.

• Reproductive and Developmental Biology
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• v.41 no.1
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• pp.7-16
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• 2017

The knock-in efficiency in the fibroblast is very important to produce transgenic domestic animal using nuclear transfer. In this research, we constructed three kinds of different knock-in vectors to study the efficiency of knock-in depending on structure of knock-in vector with different size of homologous arm on the ${\beta}-casein$ gene locus in the somatic cells; DT-A_cEndo Knock-in vector, DT-A_tEndo Knock-in vector I, and DT-A_tEndo Knock-in vector II. The knock-in vector consists of 4.8 kb or 1.06 kb of 5' arm region and 1.8 kb or 0.64 kb of 3' arm region, and neomycin resistance gene(neor) as a positive selection marker gene. The cEndo Knock-in vector had 4.8 kb and 1.8 kb homologous arm. The tEndo Knock-in vector I had 1.06 kb and 0.64 kb homologous arm and tEndo Knock-in vector II had 1.06 kb and 1.8 kb homologous arm. To express endostatin gene as transgene, the F2A sequence was fused to the 5' terminal of endostatin gene and inserted into exon 7 of the ${\beta}-casein$ gene. The knock-in vector and TALEN were introduced into the bovine fibroblast by electroporation. The knock-in efficiencies of cEndo, tEndo I, and tEndo II vector were 4.6%, 2.2% and 4.8%, respectively. These results indicated that size of 3' arm in the knock-in vector is important for TALEN-mediated homologous recombination in the fibroblast. In conclusion, our knock-in system may help to create transgenic dairy cattle expressing human endostatin protein via the endogenous expression system of the bovine ${\beta}-casein$ gene in the mammary gland.