• 농촌의학ㆍ지역보건
• /
• 제15권1호
• /
• pp.49-55
• /
• 1990

Zoonoses are those diseases and infections that are naturally transmitted between vertebrate animals and man. At present, approximately 175 diseases caused by virus, rickettsia, bacteria, protozoa and helminthes as well as some of arthropods and the number is continually increasing. Although zoonoses are generally recognized as significant health problems, the actual prevalence and incidence of zoonotic infections is difficult to determine. Medical services in a rural area are scarce and ill persons may have little chance to see a physician. Furthermore, a lack of physician awareness and of appropriate diagnostic support is made often misdiagnosis. In rural Korea, parasitic disease is still important subject in the field of zoonotic infections considering its number and morbidity. Recently there was conspicuous reduction of soil-transmitted parasitic diseases (i.e. ascariasis. hookworms, trichuriasis), but clonorchiasis. intestinal trematodiases and taeniasis are a considerable problem. This is often significantly related to human behavioral patterns based on socio-economic and cultural conditions and linked with the local biologic and physical environment. The most of all parasitic zoonoses to man and animals are the food-transmitted parasitic diseases, and domestic animals such as dog, cat, pig, as well as fat and some mammals, ruminants and birds are act as reservoir hosts. In the present topics, current problems of parasitic zoonoses caused by protozoan infections (i. e. toxoplasmosis, pneumocystosis and cryptosporidiosis) which are the most common opportunistic in AIDS or immunocompromised persons, trematode infections (i.e. clonorchiasis, paragonimiasis and some intestinal tremadodiases). cestode infections such as cysticercosis and sparganosis, and some nematode infections of anisakiasis, thelaziasis and strongyloidiasis, are discussed here those on the occurrence in man and animals and the source of transmission as well as prevention and control measures in Korea.

• Parasites, Hosts and Diseases
• /
• 제54권6호
• /
• pp.703-710
• /
• 2016

The trichomonad species Tritrichomonas foetus and Pentatrichomonas hominis were recently detected in the feces of dogs with diarrhea. However, little information is available on the prevalence and pathogenicity of these parasites in the canine population. Therefore, the aim of this study was to determine the prevalence and molecular characterization of trichomonads infecting pet dogs in Anhui and Zhejiang provinces, east China. In total, 315 pet dogs, with or without diarrhea, from 7 pet hospitals were included in this epidemiological survey. Microscopy and PCR detected P. hominis in 19.7% (62/315) and 31.4% (99/315) of fecal samples, respectively. T. foetus infection was detected in 0% (0/315) of samples with microscopy and in 0.6% (2/315) with PCR. The prevalence of P. hominis was significantly higher in young dogs (${\leq}12months$) than in adult dogs (>12 months), and was significantly higher in diarrheic dogs (50.6%) than in non-diarrheic dogs (24.3%; P<0.05). Infection with T. foetus did not correlate with any risk factors evaluated in this study. A sequence analysis of the P. hominis PCR products showed minor allelic variations between our sequences and those of P. hominis strains from other hosts in different parts of the world. Type CC1 was the most common strain in dogs in east China. The internal transcribed spacer 1 (ITS1)-5.8S rRNA gene sequences from the 2 T. foetus isolates detected in this study displayed 100% identity and were homologous to the sequences of other strains isolated from domestic cats in other countries.

• 한국수정란이식학회지
• /
• 제18권2호
• /
• pp.85-90
• /
• 2003

본 연구는 개 난소의 수송온도가 난자의 생존률에 미치는 영향과 체외성숙 배양시 성숙률에 미치는 영향에 대하여 검토하였다. 1. 개 난소를 4$^{\circ}C$와 38$^{\circ}C$에 저장 후 5시간 내로 연구실로 운반한 난소에서 채취한 난자를 체외배양 하여 생존율을 조사한 결과 체외배양 24시간째 13.2%(15/114), 77.8%(105/135)의 생존율을 보였으며, 48시간째는 0% (0/129), 72.9% (129/177) 의 생존율을 나타내어 38$^{\circ}C$에 수송한 난소에서 채취한 난자가 4$^{\circ}C$에 수송한 난소에서 채취한 난자보다 유의적으로 높은 생존율을 보였다. 2. 체외성숙 배양 24, 48, 96 시간 체외배양하여 핵 성숙율을 확인한 결과 MI∼MII까지의 성숙율이 8.3% (6/72), 8.9% (9/101), 9.5% (8/84)로 체외배양 시간을 96시간까지 연장하여도 성숙률이 증가하지 않았다.

• 대한수의학회지
• /
• 제39권3호
• /
• pp.513-522
• /
• 1999

DNA hybridization assay using probes prepared from liver was carried out to identify species characterization of the domestic animals. Gel electrophoresis showed that the target DNA extracted from raw muscle were 1kb and uniform pattern while fragments size of heated muscle were irregular. Hybridization was performed by adding 200ng/ml probe in hybridization solution and incubating for 12 hours at $68^{\circ}C$. To obtain good discrimination, applied washing buffer and washing step differently depending on the species. The probes of pig, horse and dog formed the specific hybrids with each target DNA respectively. Although cross reaction was detected in cattle, goat and sheep but signal intensity among these species made the discrimination possible each other. Such pattern was the same in the cases of chicken, turkey and duck. The hybridization pattern of heated muscle was similar to that of raw muscle in general, but the signal intensity was inferior to that of raw muscle. Species identification between closely related animal species, hybridized using the target DNA of such closely related animal species as a blocking agent, remarkable increase of discrimination from the evident decrease of non specific reaction compared with the control group. In addition, in the admixture where certain meat was included in the beef, pork, chicken meat, we could find whether any unjust meat was admixed or not. In this case, detection limit of certain meat in admixture was 1%.

• 한국동물위생학회지
• /
• 제35권2호
• /
• pp.99-104
• /
• 2012

Otitis externa (OE) is a frequent disease in the ear canals of dogs. To identify the pathogens causing OE in dogs and to determine their antimicrobial resistances, specimens were collected from animal hospitals in Daejeon. The isolates were examined by morphological and biochemical tests, 16S rRNA analysis and antimicrobial susceptibility tests. We analyzed correlation between the isolated pathogens and external factors of dogs such as breed, age, gender, ear mite, hair in ears and experience with antibiotic therapy. Thirty three strains of bacteria were isolated from 26 of the 68 heads of dogs with OE. The most isolated bacteria were Enterococcus faecalis (E. faecalis) followed by Staphylococcus aureus (Sta. aureus), Sta. pseudointermedius, E. faecium, E. avium and Streptococcus canis (Strep. canis) in order of frequency of occurrence. Isolation frequency of Enterococcus spp. and Staphylococcus spp. were 51.5% and 45.5%, respectively. E. faecalis and E. faecium isolates showed VanB phenotype, which is resistant to vancomycin but sensitive to teicoplanin were 58% and 25%, respectively. Nine isolates among total twelve isolates of E. faecalis were isolated from the dogs treated with antibiotics. There was no methicillin-resistant Sta. aureus (MRSA), but were MR-Sta. pseudointermedius (MRSP) (57.1%) and vancomycin-resistant (VR)-Sta. pseudointermedius (14.3%) (VRSP) showing VanB phenotype. However, vanA, vanB and vanC genes were not detected in VR isolates from the dogs. Taken together, VR-Enterococcus spp. (VRE) is one of the major pathogens in domestic animals, as well as community-and hospital-acquired infection.

• Parasites, Hosts and Diseases
• /
• 제41권4호
• /
• pp.243-245
• /
• 2003

Neospora caninum is an important cause of abortion in cattle, and dogs are its only known definitive host. Its seroprevalence among domestic urban and rural dogs and feral raccoon dogs (Nyctereutes procyonoides koreensis) in Korea was studied by indirect fluorescent antibody test (IFAT) and by the neospora agglutination test (NAT), respectively. Antibodies to N. caninum were found in 8.3% of urban dogs and in 21.6% of dogs at dairy farms. Antibody titers ranged from 1:50 to 1:400. Antibodies to N. caninum were found in six (23%) of 26 raccoon dogs. However, the potential role of raccoon dogs as a source of horizontal transmission of bovine neosporosis needs further investigation. The results of this study suggest that there is a close relationship between N. caninum infection among dairy farm dogs and cattle in Korea. This study reports for the first time upon the seroprevalence of N. caninum infection in raccoon dogs in Korea.

• 한국콘텐츠학회논문지
• /
• 제22권9호
• /
• pp.388-400
• /
• 2022

본 연구는 반려동물을 돌보는 반려인 11인의 질적 인터뷰를 통해 반려동물 돌봄과 관련한 인터넷 소비 현상을 분석했다. 본 연구에서는 그동안 전혀 다루지 않았던 길고양이 돌보미를 인터뷰 대상인, 반려동물 소비자로 포함했다. 인터넷 소비는 개와 고양이의 종 차에 따라 다르게 나타났다. 개의 반려인들은 고양이에게는 필요하지 않은, 목욕제품, 미용기구, 옷, 하네스, 유모차 등을 많이 구입했다. 그동안 펫 푸어(pet-poor) 현상은 자신에게 소비할 돈을 절약하여 반려동물을 위해 소비하는 행동을 보이는 젊은 소비자들에게 많이 나타난다고 알려졌다. 그러나 이번 연구에서 펫 푸어 현상은 중장년, 노년층에서도 나타났으며 이들은 경제적으로 넉넉하지 않아도 반려견을 위해 값비싼 옷, 하네스 등을 구매하는 것으로 드러났다. 또한 중장년 여성의 길고양이 돌봄이도 펫 푸어로의 전락 가능성을 경계하고 있음을 알 수 있었다. 또한 반려동물용품의 인터넷 소비는 젠더 차이를 보인다고 할 수 없었다. 대체로 가족을 위해 생필품 구매를 주로 해 오던 여성이 반려동물용품도 온라인으로 구매했고, 부인이 인터넷 사용에 서툰 경우에는 남편이 대신 온라인으로 반려동물용품을 구매했다.

• 대한미생물학회지
• /
• 제6권1호
• /
• pp.21-27
• /
• 1971

Recent reports confirm that R factors is widespread in Korea among Enterobacteriaceae isolated from humans. However, no reports have been made concerning the incidence of transferable drug resistance in domestic animals in this country. A total of 211 isolates of Escherichia coli, including 94 strains from dogs, 76 strains from pigs, 30 strains from chickens, and 21 strains from cow milk, were examined for drug resistance and distribution of R factors. And, respective two strains of Salmonella E group and Salmonella cholerasuis which were isolated from dogs and pigs, respectively were also examined for the same purposes. Of 211 strains of E. coli isolated, 66.8% were found to be resistant to 8 antibacterial agents such as streptomycin(SM), tetracycline(TC), chloramphenicol(CP), ampicillin sodium(AP), nalidixic acid(NA), gentamicin(GM), and polymyxin B(PX). Among the isolates, 86.2% of the strains from dogs, 70% of the strains from chickens, 43.4% of the strains from pigs, and 28.6% of the strains from milk, respectively, were found to be resistant to the drugs. The following percentage of resistance of E. coli to each individual drugs was encountered: of 94 strains from dogs, AP, 64.9%; SM, 20.2%; NA, 12.8%, CP and PX, 8.5% each; GM, 2.1% each; GM, 2.1%. Among 76 strains from pigs, 42.2% and 2.6% each were resistant to TC, AP and PX, respectively. Among 30 strains from chickens, 43.3% were resistant to SM, TC, AP, respectively, and no strains were resistant to the other drugs. No strains of the isolated from milk were resistant to the drugs, except that 28.6% were resistant to SM and AP, respectively. Of the strains from dogs, multiply resistant strains(56.8%) were more than singly resistant one(43.2%) and sixteen different drug resistant patterns were observed. The most frequently encountered patterns were AP TC AP and SM CP AP NA. Of the isolates from other sources, the most frequently encountered resistant patterns were as follows: TC among the strains from pigs; SM TC AP from chickens; SM AP from milk. Of the resistant strains from dogs, 32% carried R factors and the most common resistance patterns of R factors were AP TC AP and SM TC CP, whereas 35.2% of the resistant strains from pigs carried R factors of which the most common encountered pattern was TC. Of the resistant strains from chickens, 46.9% carried R factors of which the most common patterns were SM TC TC AP and AP, whereas 50% of the resistant strains from milk carried R factors of which the most common pattern was SM. Of 4 strains of Salmonella isolated, no strains were resistant to the drugs, except that only one strain of Salmonella E group isolated from a dog was resistant to AP. The strain did not harbor R factor.

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
• /
• pp.122-122
• /
• 2003

This study examined the viability of canine oocytes following storage at 4 or $38{\circ}C$ for 5 hrs. The ovaries were collected from domestic dog following ovariohysterectomy at a local veterinary clinics and transported to laboratory In two different transport temperature at 4 or $38{\circ}C$ within 5 hrs. The cumulus oocyte complexes (COCs) were recovered after slicing with blade. In Exp. 1, the oocytes collected were matured in DMEM supplemented with l0% FBS, 0.6 mM/mlcysteine, 0.2 mM Pyruvic acid, 20 ng/ml $E_2$ and 1 $\mu g/ml$ rbST at humidified atmosphere containing 5% $CO_2 38{\circ}C$ for 24 or 48 hrs to analysis of survivability. In Exp 2, to assess nuclear development at 38{\circ}C$ group, the oocytes were matured in maturation medium for 24, 48 or 96 hrs. Survivability was judged by a morphological appearance and PI staining. Survivability rates were analyzed by General Linear Models procedure in SAS. The survival rates at 4{\circ}C$ ovary transport group showed significantly lower than at 38{\circ}C$ group (0 vs 72.9% in 48 hrs and 13.2 vs 77 8% in 24 hrs; P<0.05). The nuclear development of oocytes to MI to MII stages at 24, 48 and 96 hrs was 8.3% (6/72), 8.9% (9/101), and 9.5% (8/84). These results showed that the canine oocytes were remarkably sensitive to a low temperature and did not increase nuclear development rate depend on maturation time to 96 hrs.

• 한국수정란이식학회지
• /
• 제20권1호
• /
• pp.63-69
• /
• 2005

본 연구는 개의 미성숙난자의 낮은 성숙율을 개선하기 위하여 자연발정온 개의 생식기관에 미성숙 난자를 이식하여 체내 배양 난자를 회수하여 회수된 난자의 핵 성숙율을 조사한 결과는 다음과 같다. 1. 미성숙난자를 24, 48 및 72시간 동안 체외성숙을 유도하였을 때 성숙시간에 따른 체외성숙율에는 유의적 차이는 인정되지 않았다. 2. 체내 배양 기간에 따른 난자의 회수율은 배양기간이 길어질수록 유의적으로 낮은 회수율을 보였으며, 배양기간이 증가할수록 난자의 생존율은 유의적으로 (P<0.05) 저하되었고 사멸된 난자의 비율도 또한 증가하였다. 3. 회수된 난자는 4, 5, 6일간의 체내 배양기간에 관계없이 대부분 GV상태로 (20/34; 6/15; 4/7)존재하였으며, 성숙된 난자는 체내 배양 4일째 회수된 난자에서 $5.8\%(2/34)$의 난자가 M II까지 발달한 것으로 조사되어 개의 미성숙난자의 체내 배양은 개 난자의 핵성숙을 개선하지 못하였다. 이상의 결과에서와 같이 개의 미성숙난자의 체내 배양은 M II 단계까지의 발달에 기여하지 못한 것으로 판단되며, 이는 개 미성숙난자의 핵발달은 난포에서의 자극과 활성화가 요구되는 것으로 판단된다.