Browse > Article

Effect of Meiotic Maturation of Canine Oocytes Cultured in Reproductive Tract  

Lee H. S. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University)
Lee Y. H. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University)
Yin X. J. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University)
Kong I. K. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University)
Publication Information
Journal of Embryo Transfer / v.20, no.1, 2005 , pp. 63-69 More about this Journal
Abstract
This study were carried out to evaluate the possibility of nuclear progression of canine immature oocytes, of which was cultured in a reproductive tract, such as oviduct, ovarian bursa and uterus of estrus bitch for 4, 5 and 6 days following immediately collection. Cumulus intact oocytes(COC) fore collected from domestic dog following ovariohysterectomy at local veterinary clinics. In Exp. 1, COCs $of>110\;{\mu}m$ diameter were selected and cultured in vitro at $39^{\circ}C$, $5\%\;CO\_{2} $ in air atmosphere. The nuclear progression of canine oocytes checked at 24, 48 or 72 h after in vitro maturation. There was not increased the nuclear progression to the M II stage depending on culture periods at 24, 48 and 72h $(1.3\%,\;3.7\%\;and\;4.7\%)$. In Exp. 2, to evaluate of nuclear progression of immature oocytes, collected or in vitro cultured oocytes were transfer into a canine reproductive tract (oviduct, ovarian bursa and uterus). The recovery rates of canine oocytes from a reproductive tract after 4 days $(33.7\%)$ in vivo culture were significantly higher than those 5 $(17.7\%)$ 6 day $(3.4\%)$ (P<0.05). The survival rates of collected oocytes after 4 days $(60.0\%)$ were also significantly higher than those of 5 days $(30.2\%)$ and 6 days $(38.9\%)$ (P<0.05). The meiotic resumption rates of canine oocytes were not significantly difference among the culture periods at 4 days $(5.9\%)$, 5 days $(0.0\%)$ and 6 days $(0.0\%)$. These results show that the nuclear progression of canine immature oocytes from in in vivo culture was not affect the nuclear resumption of oocytes.
Keywords
canine; oocyte; oocyte transfer; in vivo maturation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bolamba D, Russ KD, Olson MA, Sandler JL and Durrant BS. 2002. In vitro maturation of bitch oocytes from advanced preantral follicles in synthetic oviduct fluid medium: serum is not essential. Theriogenology, 58(9): 1689-1703   DOI   ScienceOn
2 Hinrichs K, Love CC, Brinsko SP, Choi YH and Varner DD. 2002. In vitro fertilization of in vitro-matured equine oocytes: effect of maturation medium, duration of maturation, and sperm calcium ionophore treatment, and comparison with rates of fertilization in vivo after oviductal transfer. Biol. Reprod., 67(1):256-262   DOI   ScienceOn
3 Saint-Dizier M, Reynaud K and Chastant-Maillard S. 2004. Chromatin, microtubules, and kinases activities during meiotic resumption in bitch oocytes. Mol. Reprod. Dev., 68(2):205-212   DOI   ScienceOn
4 Mahi CA and Yanagimachi R. 1976. Maturation and sperm penetration of canine ovarian oocytes in vitro. J. Exp. Zool., 196(2):189-196   DOI   ScienceOn
5 Wirtu G, Bailey TL, Chauhan MS, Parker NA, Dascanio JJ, Gwazdauskas FC and Ley WB. 2004. Xenogenous fertilization of equine oocytes following recovery from slaughterhouse ovaries and in vitro maturation. Theriogenlogy, 61:381-391   DOI   ScienceOn
6 Sirard MA, Lambert RD, Menard DP and Bedoya M. 1985. Pregnancies after in-vitro fertilization of cow follicular oocytes, their incubation in rabbit oviduct and their transfer to the cow uterus. J. Reprod. Fertil., 75(2):551-556   DOI   ScienceOn
7 Kim MK, Fibrianto YH, Oh HJ, Jang G, Kim HJ, Lee KS, Kang SK, Lee BC and Hwang WS. 2005. Effects of estradiol-17beta and progesterone supplementation on in vitro nuclear maturation of canine oocytes. Theriogenology, 63(5):1342-1353   DOI   ScienceOn
8 Yamada S, Shimazu Y, Kawano Y, Nakazawa M, Naito K and Tyoda Y. 1993. In vitro maturation and fertilization of preovulatory dog oocytes. J. Reprod. Fertil. Suppl., 47:227-229
9 Feldman E and Nelson R. 1996. Ovarian cycle and vaginal cytology. In canine and feline endocrinology and reproduction. 2nd ed., WB Saunders Company, pp. 526-546
10 Concannon P, Tsutsui T and Shille V. 2001. Embryo development, hormonal requirements and maternal responses during canine pregnancy. J. Reprod. Fertil. Suppl., 57:169-179
11 Luvoni GC, Luciano AM, Modina S and Gandolfi F. 2001. Influence of different stages of the oestrous cycle on cumulus-oocyte communications in canine oocytes: effects on the efficiency of in vitro maturation. J. Reprod. Fertil. Suppl., 57:141-146
12 Willingham-Rocky LA, Hinrichs K, Westhusin ME and Kraemer DC. 2003. Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro. Reproduction, 126(4):501-508   DOI   PUBMED   ScienceOn
13 Tsutsui T. 1989. Gamete physiology and timing of ovulation and fertilization in dogs. J. Reprod. Fertil. Suppl., 39:269-275   PUBMED
14 Lee HS, Yin XJ, Lee YH, Kang TY and Kong IK. 2003. Effect of IVM medium and protein source on in vitro maturation of canine oocytes. Korean J. Emb. Trans., 18(1):75-80
15 Farstad W. 2000. Assisted reproductive technology in canid species. Theriogenology, 53(1):175-186   DOI   PUBMED   ScienceOn
16 Fukui Y, Urakawa M, Sasaki C, Chikamatsu N and Ono H. 1989. Development to the late morula or blastocyst stage following in vitro maturation and fertilization of bovine oocytes. Anim. Reprod. Sci., 18:139-148   DOI   ScienceOn
17 Renton JP, Boyd JS, Eckersall PD, Ferguson JM, Harvey MJ, Mullaney J and Perry B. 1991. Ovulation, fertilization and early embryonic development in the bitch (Canis familiaris). J. Reprod. Fertil., 93(1):221-231   DOI   ScienceOn
18 Otoi T, Ooka A, Murakami M, Karja NW and Suzuki T. 2001. Size distribution and meiotic competence of oocytes obtained from bitch ovaries at various stages of the oestrous cycle. Reprod. Fertil. Dev., 13(2-3):151-155   DOI   ScienceOn
19 de Avila Rodrigues B and Rodrigues JL. 2003. Influence of reproductive status on in vitro oocyte maturation in dogs. Theriogenology, 60(1):59-66   DOI   ScienceOn
20 Hewitt DA and England GC. 1999. Synthetic ovi-ductal fluid and oviductal cell coculture for canine oocyte maturation in vitro. Anim. Reprod. Sci., 55(1):63-75   DOI   ScienceOn
21 Rodrigues BA and Rodrigues JL. 2003. Meiotic response of in vitro matured canine oocytes under different proteins and heterologous hormone supplementation. Reprod. Domest. Anim., 38(1):58-62   DOI   ScienceOn
22 Otoi T, Fujii M, Tanaka M, Ooka A and Suzuki T. 1999. Effect of serum on the in vitro maturation of canine oocytes. Reprod. Fertil. Dev., 11(7-8):387-390   DOI   ScienceOn