• Title/Summary/Keyword: domestic dog

Search Result 84, Processing Time 0.036 seconds

Intraerythrocytic culture and development of serological diagnostic tests of Babesia gibsoni 1. Indirect fluorescent antibody test and enzyme-linked immunosorbent assay for antibody detection of Babesia gibsoni infections in dogs (Babesia gibsoni의 적혈구내 배양법과 진단법 개발에 관한 연구 1. Babesia gibsoni 진단을 위한 간접형광항체법(IFAT)과 효소표지면역검사법(ELISA))

  • Suh, Myung-deuk;Shin, Yong-seung
    • Korean Journal of Veterinary Research
    • /
    • v.37 no.3
    • /
    • pp.583-593
    • /
    • 1997
  • Indirect fluorescent antibody test(IFAT) and enzyme-linked imuunosorbent assay (IgG-ELISA) as serological diagnostic tools were conducted to evaluate the usefulness for diagnosis of canine babesiosis infected with Babesia gibsoni in domestic various dog breeds, american pit bullterrier, military shepherd, and mongrel dogs. The results obtained from this study were abstracted as follows. The nonionic detergent Triton X-100 and absorbent bio-bead $SM_2$ were useful reagents for the preparation of pure merozoite antigen of B gibsoni to be used in ELISA. The optimum reaction in ELISA was shown when the protein concentration of ELISA antigen was measured as 625ng/ml and the conjugate concentration was diluted into 1/6000 fold. The average OD value of ELISA in sera determined with negative responses in IFAT was measured as $0.255{\pm}0.051$(490nm) and the cut - off value of OD was determined as 0.399(490nm). The serum antibodies in both of IFAT and ELISA were detected on one week after artificially infected with B gibsoni and these high antibody titers, 512X in IFAT and 1024X in ELISA, were long lasted until 15 weeks after infection. The reproducibility of reaction and stability of the antigen absorbed microtitration polystyrene plate preserved in $4^{\circ}C$ refrigerator and $-20^{\circ}C$ freezer, respectively could be lasted until 135 days after storage. The positive rates in IFAT by dog breeds were shown 8.1%(60/744 heads) in mongrel dogs, 81.3%(78/96 heads) in american pit bullterrier and 15.6%(15/96 heads) in military shepherd, while the positive rate in ELISA shown 17.6%(131/744 heads) in mongrel dogs, 83.3%(80/96 heads) in american pit bullterrier and 36.5%(35/96 heads) in military shepherd, respiectively. In the total of 936 heads surveyed with IFAT and ELISA the positive rates in IFAT and ELISA were 16.4%(153/936 heads) and 26.3%(246/936 heads), respectivily. Agreement of reactions between IFAT and ELISA was shown 82.4% in 936 dog sera. The specificity and sensitivity of ELISA reaction were 83.5% and 76.5%, respectively. From the conclusion obtained in this study it was evaluated that IFAT and ELISA were useful as highly specific, sensitive and stable serelogical tools for the diagnosis of canine babesiosis in Korea.

  • PDF

Cytokine Production in Canine PBMC after Bartonella Henselae Infection (Bartonella Henselae 감염 후의 개 말초혈액단핵구에서의 사이토카인 양상)

  • Choi, Eun-Wha;Lee, Jong-Hwa;Koo, Hye-Cheong;Park, Yong-Ho;Youn, Hwa-Young
    • Journal of Veterinary Clinics
    • /
    • v.27 no.4
    • /
    • pp.311-314
    • /
    • 2010
  • Bartonella henselae is the causative agent of cat scratch disease. Although cats are the main zoonotic reservoirs of Bartonella spp., unusual cases of cat scratch disease caused by a domestic dog scratch have been recently reported. For the in vivo B. henselae infection, eight dogs were inoculated intradermally with $2{\times}10^8CFU$ of B. henselae Houston-1 suspended in 1 ml of phosphate buffered saline on day 0 and subsequent injections of the same amount given intradermally on days 21, 28, 36, 58 and 64. After in vivo canine B. henselae infection was confirmed by nested PCR, the IFN-$\gamma$ levels of the culture supernatant of PBMC stimulated with B. henselae was significantly higher in the B. henselae-PCR positive group than the B. henselae-PCR negative group. Our results showed that the canine immune responses against B. henselae were different from those of cats. Th1 activation by B. henselae stimulation was characterized in dog peripheral blood mononuclear cells, whereas Th2 activation was reported in B. henselae-infected cats.

Strategic Direction and Road Map of Expanding Prevention of Winter Disease in the Summer (동병하치 확산을 위한 전략적 방향과 이행방안)

  • Song, Ho-Sueb
    • Journal of Acupuncture Research
    • /
    • v.27 no.3
    • /
    • pp.147-157
    • /
    • 2010
  • Objectives : The purpose of this study was to propose appropriate strategic directions and road maps for successful achievement of programs preventing winter disease in the summer. Methods : Details on programs preventing winter disease in the summer such as clear concept, theoretical basis, current status, intervention or available prescriptions and indication/contraindication/caution were prepared through the related journal review, upon which an observational study was devised and done for simulation to find out even a trivial problem and to guarantee the safety beforehand. The experimental group was divided into 5 groups by the size of pill and the way ginger is treated; 1cm pill with ginger group, 3cm pill group without ginger, 3cm pill group dipped into ginger, 3cm pill group applying ginger to acupoints and 3cm pill group with ginger Results 1. program preventing winter disease in the summer was defined as representative winter diseases such as common cold, influenza, chronic asthma, chronic bronchitis, allergic rhinitis, emphysema, chronic gastritis and rheumatoid arthritis, and preventive care in the summer, reinforcing deficient yang qi of five viscera by using exuberant yang qi from summer heat. 2. It was based upon historically established theories which is 'nourishing yang qi in the spring and summer', 'long summer, namely rainy spell in the summer overwhelms the winter, because of earth winning water according to the five phases theory' and 'To replenish yang qi is major principle to treat winter diseases, which can be most appropriately and timely applied to the patient with deficient yang qi of five viscera inherently, especially in the three dog days of the summer, because of exuberant exterior yang qi and deficient interior yang qi in the five viscera'. 3. In the adjacent China and Taiwan, acupoint applying method in the three dog days named 'San Fu Tie' have been stirring a boom throughout the nation, in which Xiaochuan Gao was used as a basic prescription and it mainly was applied at bilateral $BL_{13}$, $_{15}$ and $_{17}$ for about 4 hours. As far as domestic current status, the necessity of adopting the above method prior to Herbal formula was also recognised, because not a few koreans have apprehension for the safety of it including medicinal herbs and are reluctant to take it any more due to negative advertisement of narrow minded doctors' association. 4. Indication of acupoint applying method in the three dog days included most of winter diseases such as common cold, influenza, chronic asthma, chronic bronchitis, allergic rhinitis, emphysema, chronic gastritis. contraindication was pregnant woman and the weak such as infants and the old. More attention was paid to grasp firmly the normal reaction following the treatment for preventing side effect and teasing blister. recommendation was also given to abstain from food inducing phlegm and dampness such as meat, shrimp and crab as well as cold drinks and foods 5. In the simulation observational study based upon the above findings following review the related articles, no blister was shown on the acupoints icluding bilateral $BL_{13}$, $_{15}$ and $_{17}$ in every experimental group during 24hr observation following the acupoint applying treatment with pills made by modified and devised prescription. At 4 hr, the effectiveness of it reached a peak showing redness and mild tenderness and there is little difference between groups 3cm pills groups regardless of the way ginger was treated. abdominal distention and growling was found in all the volunteers during the treatment at CV 8. Strategic directions and road maps : Through successful fulfillment of the program preventing winter disease in the summer, Korean traditional medicine should be integrated into mainstream national health care services. Cultural access was thought to be as important as Scientific EBM approach. First of all, To evoke potential cultural homogeneity from campaigns and press advertisement was needed for promoting public awareness about preventing winter disease in the summer by enhancing immunity via acupoint applying treatment in the three dog days, and then indigenous name as Sambokcheop, protocol, Clinical Research Form for data collection of it should be developed and prepared. Once the first step was taken this summer, through a thorough data collection and scrutinized scientific evaluation, drawbacks should be compensted for and the efficacy and safety should be substantiated.

Epidemiological Investigation of Asymptomatic Dogs with Leishmania Infection in Southwestern China Where Visceral Leishmaniasis is Intractable

  • Zhao, Gui-Hua;Yin, Kun;Zhong, Wei-Xia;Xiao, Ting;Wei, Qing-Kuan;Cui, Yong;Liu, Gong-Zhen;Xu, Chao;Wang, Hong-Fa
    • Parasites, Hosts and Diseases
    • /
    • v.54 no.6
    • /
    • pp.797-801
    • /
    • 2016
  • Heishui county, located in northwest Sichuan province, southwestern China, is an endemic area of zoonotic visceral leishmaniasis (VL) and is the most intractable area. VL is never destroyed in it. Asymptomatic dogs (Leishmania parasites have been diagnosed but clinically healthy) are considered to be a potential reservoir host in zoonotic VL area, and most can lead to infection of individuals, that is a new challenge for controlling VL in humans. The present study aimed to assess the Leishmania infection rate of asymptomatic dogs in Heishui county. Total 105 asymptomatic domestic dogs were gathered from 4 districts in Heishui county to investigate the infection rate with serological and molecular methods based on ELISA and kinetoplast minicircle DNA(kDNA) PCR, respectively. Out of 105 dogs, 44 (41.9%) were positive by more than 1 method; 21 (20.0%) were positive by ELISA, and 30 (28.6%) were positive by kDNA-PCR. Our study showed that Leishmania infection of domestic dogs which is clinically healthy is prevalent in the studied district, and the asymptomatic dogs infected by Leishmania may be the primary reason for the prevalence of visceral leishmaniasis in the area.

A Study on Application Plan and Research Direction Proposal of Domestic Therapy Agriculture through Review of Animal-Assisted Therapy (동물매개치유 연구고찰을 통한 국내 치유농업의 방향설정과 적용방안 연구)

  • Kim, Dae-Sik;Lee, Young-Ran;Park, Hee-Geun;Pipit, Pitriani;Li, Wei;Yi, Gwang-Min;Jung, Sung-Gu;Park, Jong-Gu;Kim, Kyung-Il;Lee, Wang-Lok
    • Journal of Korean Society of Rural Planning
    • /
    • v.22 no.1
    • /
    • pp.57-67
    • /
    • 2016
  • The purpose of this study was to review of animal-assisted therapy. Method: Animal-assisted therapies (AATs) in domestic and international studies (68 cases) were analyzed by searching databases, KERIS & KISS, and the Pub MED(NCBI). Result: In AATs, a few animals were used for the various disease or disorder. Dog centered therapies were most common.. AATs were used in the various disorder and disease. For the psychological effect, majority of the outcome variables were depression and anxiety. For the physical effect, the most outcome variables were blood pressure, heart rate, and stress hormone. However, These results show that AATs were confined to specific areas such as sensation-centered therapies. Conclusion: AATs had positive effects both in physical and psychological factors even if the study areas have not been applied to the various condition. The further research is needed to identify scientific grounds for the effects of the interventions through various therapies.

Prevalence of Intestinal Helminth Infections in Dogs and Two Species of Wild Animals from Samarkand Region of Uzbekistan

  • Yong, Tai-Soon;Lee, Kyu-Jae;Shin, Myeong Heon;Yu, Hak Sun;Suvonkulov, Uktamjon;Sergeevich, Turycin Bladimir;Shamsiev, Azamat;Park, Gab-Man
    • Parasites, Hosts and Diseases
    • /
    • v.57 no.5
    • /
    • pp.549-552
    • /
    • 2019
  • This study aimed to determine the prevalence of intestinal helminth parasitic infections and associated risk factors for the human infection among the people of Samarkand, Uzbekistan. Infection status of helminths including Echinococcus granulosus was surveyed in domestic and wild animals from 4 sites in the Samarkand region, Uzbekistan during 2015-2018. Fecal samples of each animal were examined with the formalin-ether sedimentation technique and the recovery of intestinal helminths was performed with naked eyes and a stereomicroscope in total 1,761 animals (1,755 dogs, 1 golden jackal, and 5 Corsac foxes). Total 658 adult worms of E. granulosus were detected in 28 (1.6%) dogs and 1 (100%) golden jackal. More than 6 species of helminths, i.e., Taenia hydatigena, Dipylidium caninum, Diplopylidium nolleri, Mesocestoides lineatus, Toxocara canis, and Trichuris vulpis, were found from 18 (1.0%) dogs. Six (T. hydatigena, Toxascaris leonina, Alaria alata, Uncinaria stenocephala, D. caninum, and M. lineatus) and 2 (D. nolleri and M. lineatus) species of helminths were also detected from 5 Corsac foxes and 1 golden jackal, respectively. Taeniid eggs were found in 2 (20%) out of 10 soil samples. In the present study, it was confirmed that the prevalences of helminths including E. granulosus are not so high in domestic and wild animals. Nevertheless, the awareness on the zoonotic helminth infections should be continuously maintained in Uzbekistan for the prevention of human infection.

Ticks Collected from Wild and Domestic Animals and Natural Habitats in the Republic of Korea

  • Kim, Baek-Jun;Kim, Hyewon;Won, Sohyun;Kim, Heung-Chul;Chong, Sung-Tae;Klein, Terry A.;Kim, Ki-Gyoung;Seo, Hong-Yul;Chae, Joon-Seok
    • Parasites, Hosts and Diseases
    • /
    • v.52 no.3
    • /
    • pp.281-285
    • /
    • 2014
  • Ticks were collected from 35 animals from 5 provinces and 3 metropolitan cities during 2012. Ticks also were collected by tick drag from 4 sites in Gyeonggi-do (2) and Jeollabuk-do (2) Provinces. A total of 612 ticks belonging to 6 species and 3 genera were collected from mammals and a bird (n=573) and by tick drag (n=39). Haemaphyalis longicornis (n=434) was the most commonly collected tick, followed by H. flava (158), Ixodes nipponensis (11), Amblyomma testudinarium (7), H. japonica (1), and H. formosensis (1). H. longicornis and H. flava were collected from all animal hosts examined. For animal hosts (n>1), the highest Tick Index (TI) was observed for domestic dogs (29.6), followed by Siberian roe deer (17.4), water deer (14.4), and raccoon dogs (1.3). A total of 402 H. longicornis (adults 86, 21.4%; nymphs 160, 39.8%; larvae 156, 38.9%) were collected from wild and domestic animals. A total of 158 H. flava (n=158) were collected from wild and domestic animals and 1 ring-necked pheasant, with a higher proportion of adults (103, 65.2%), while nymphs and larvae only accounted for 12.7% (20) and 22.2% (35), respectively. Only 7 A. testudinarium were collected from the wild boar (6 adults) and Eurasian badger (1 nymph), while only 5 I. nipponensis were collected from the water deer (4 adults) and a raccoon dog (1 adult). One adult female H. formosensis was first collected from vegetation by tick drag from Mara Island, Seogwipo-si, Jeju-do Province.

The Detection of Helicobacter-like Organisms in Dogs (개에서 Helicobacter-like organism의 검출)

  • An, Joong-Ho;Nam, Heon-Woo;Han, Jung-Hee;Kim, Doo
    • Journal of Veterinary Clinics
    • /
    • v.16 no.2
    • /
    • pp.281-288
    • /
    • 1999
  • Helicobacter species have been identified in or isolated from domestic carnivores, but their prevalence in different population of animals and their clinical significance are still unknown. This study was peformed to evaluate the prevalence of Helicobacter in clinically healthy dogs by urease test, culture, morphological examination and polymerase chain reaction (PCR) technique. Tissue samples from 70 dogs in Kangwon and Kyunggi areas from August 1998 to April, 1999, were examined. The detection rates of Helicobacter by urease activity of tissue-samples were 84.6%, 61.3% and 4.8 % in the fundus, the antrum and the duodenum, respectively. One strain of Helicobacter was isolated from the duodenum. It was identified as H canis by biochemical and morphorogical examination. The detection rates of Helicobacter by histological examination were 92.3%, 79.0% and 4.8% in the fundus, antrum and the duodenum, respectively. Helicobacter organisms were colonized more in the gastric pits than in the surface of epithelium, the gastric gland or the parietal cell. Although most of dogs were colonized with Helicobacter in tissue, gross lesions and specific histopathological lesions caused by Helicobacter in these tissues were not observed. The detection rate of Helicobacter by PCR was 78.6%. The histological examination was more sensitive than urease test, culture or PCR technique for the detection of Helicobacter.

  • PDF

The Perception and the Consumption Behavior for the Meats in Koreans (한국인의 육류음식에 관한 의식구조 및 이용행동)

  • 윤계순;우자원
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.28 no.1
    • /
    • pp.246-256
    • /
    • 1999
  • This study was undertaken to investigate the perception and the consumption behavior for the meats in Koreans. The subjects were 491 Koreans including the ones residing in New Zealand. Most of subjects answered the meats are used for supper time. The frequency of the meats intake was affected by education and monthly income level. 69.2% of the subjects had positive response such as increased stamina and fulled stomach for psychological state after the meats intake. The use of meats recently revealed that 48% of the subjects have decreased its consumption. The main reasons diminished the use of meats were for the good health and economic state. Women were found to avoid the meats fat to a greater degree than men. The kinds of the meat used frequently were beef, pork and chicken. The younger tended to higher the frequency of the processed meats intake than the older. According to 3 level of BMI of the subjects, there were significant differences in the amount of the meat intake and perception for necessity of the meat. This study showed that the higher the preference for meat, the education and the monthly income level, the more the meat intake frequency. There were not significant differences between Koreans residing in domestic and New Zealand except the dog meat in the perception and consumption of the meat.

  • PDF

The outbreaks and surveillance of animal rabies in Gangwon-do

  • Shin Myung-Kyun;Shin Eun-Kyung;Chung Dong-Soo;Cheong Ki-Soo;Park Young-Nam;Choi Jee-Hee
    • Korean Journal of Veterinary Service
    • /
    • v.27 no.4
    • /
    • pp.335-343
    • /
    • 2004
  • Although human rabies deaths are rare, the disease remains a public health problem in Korea. Here we report the outbreaks and surveillance of animal rabies in Gangwon-do. Animal rabies infections were identified in 119 animals from 1993 to 2003. The $78\%$ of all rabid animals were domestic species in Gangwon-do. Wild Korean raccoon dog (N. p. koreensis) continued to be the only reported rabid wildlife species. Outbreaks of rabies infections in Korean raccoon dogs are found in broad geographic regions across the northern Gangwon-do. The principal rabies hosts today are probably wild animals in Gangwon-do. The malaise, cerebral dysfunction, anxiety, confusion, agitation and abnormal behavior of the animals were the important symptoms of the disease. The Encephalitis, infiltration with lymphocytes and polymorphonuclear leukocytes and the inclusion bodies (Negri bodies) in neuronal cells were the specific histopathological signs. The results of indirect fluorescent antibody test (IFA) for animal rabies diagnosis were identical and the technique was useful to diagnose the disease. Preexposure vaccination is recommended for persons in high-risk groups, such as laboratory workers, veterinarians and certain animal handlers.