Browse > Article
http://dx.doi.org/10.3347/kjp.2016.54.6.797

Epidemiological Investigation of Asymptomatic Dogs with Leishmania Infection in Southwestern China Where Visceral Leishmaniasis is Intractable  

Zhao, Gui-Hua (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Yin, Kun (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Zhong, Wei-Xia (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Xiao, Ting (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Wei, Qing-Kuan (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Cui, Yong (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Liu, Gong-Zhen (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Xu, Chao (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Wang, Hong-Fa (Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences)
Publication Information
Parasites, Hosts and Diseases / v.54, no.6, 2016 , pp. 797-801 More about this Journal
Abstract
Heishui county, located in northwest Sichuan province, southwestern China, is an endemic area of zoonotic visceral leishmaniasis (VL) and is the most intractable area. VL is never destroyed in it. Asymptomatic dogs (Leishmania parasites have been diagnosed but clinically healthy) are considered to be a potential reservoir host in zoonotic VL area, and most can lead to infection of individuals, that is a new challenge for controlling VL in humans. The present study aimed to assess the Leishmania infection rate of asymptomatic dogs in Heishui county. Total 105 asymptomatic domestic dogs were gathered from 4 districts in Heishui county to investigate the infection rate with serological and molecular methods based on ELISA and kinetoplast minicircle DNA(kDNA) PCR, respectively. Out of 105 dogs, 44 (41.9%) were positive by more than 1 method; 21 (20.0%) were positive by ELISA, and 30 (28.6%) were positive by kDNA-PCR. Our study showed that Leishmania infection of domestic dogs which is clinically healthy is prevalent in the studied district, and the asymptomatic dogs infected by Leishmania may be the primary reason for the prevalence of visceral leishmaniasis in the area.
Keywords
Leishmania; epidemiology; asymptomatic dog; China;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Wang JY, Ha Y, Gao CH, Wang Y, Yang YT, Chen HT. The prevalence of canine Leishmania infantum infection in western China detected by PCR and serological tests. Parasit Vectors 2011; 4: 69.   DOI
2 Wang JY, Chen SB, Gao CH, Jin CF, Feng Y, Zhang CJ, He HX, Yang CM, Yang YT, Bao YF. Survey on the Leishmania infantum asymptomatic infection in dogs in Wenxian county of Gansu province. Chin J Zoonoses 2006; 22: 734-737.
3 Dantas-Torres F, Brandao-Filho SP. Visceral leishmaniasis in Brazil: revisiting paradigms of epidemiology and control. Rev Inst Med Trop Sao Paulo 2006; 48: 151-156.   DOI
4 Otranto D, Paradies P, de Caprariis D, Stanneck D, Testini G, Grimm F, Deplazes P, Capelli G. Toward diagnosing Leishmania infantum infection in asymptomatic dogs in an area where leishmaniasis is endemic. Clin Vaccine Immunol 2009; 16: 337-343.   DOI
5 Wang JY, Cui G, Chen HT, Zhou XN, Gao CH, Yang YT. Current epidemiological profile and features of visceral leishmaniasis in People's Republic of China. Parasit Vectors 2012; 5: 31.   DOI
6 Zhang FN, Xiao N, Chen YL. Prevalent trends and control strategy of leishmaniasis in Sichuan province in 2004-2008. Chin Trop Med 2009; 9: 1836-1838.
7 Moshfe A, Mohebali M, Edrissian G, Zarei Z, Akhoundi B, Kazemi B, Jamshidi S, Mahmoodi M. Canine visceral leishmaniasis: asymptomatic infected dogs as a source of L. infantum infection. Acta Trop 2009; 112: 101-105.   DOI
8 Zhang FN, Zhang LP, Zhang YZ. Analysis on risk factors of leishmaniasis in quake stricken endemic areas in Sichuan. J Prev Med Infect 2010; 6: 433-437.
9 Zhang FN, Li GR, Lei Y, Chen YL. Analysis on leishmaniasis in Sichuan province from 1984 to 2005. J Pathog Biol 2007; 2: 79-80.
10 Gramiccia M, Gradoni L. The current status of zoonotic leishmaniasis and approaches to disease control. Int J Parasitol 2005; 35: 1169-1180.   DOI
11 Zhao GH, Yin K, Zhong WX, Cui Y, Wang HF. Development and application of rapid molecular method for detection of asymptomatic infection of Leishmania. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi 2015; 27: 45-48, 52.
12 Wang JY, Ha Y, Gao CH, Wang Y, Yang YT, Chen HT. The prevalence of canine Leishmania infantum infection in western China detected by PCR and serological tests. Parasit Vectors 2011; 4: 69.   DOI
13 Bhang DH, Choi US, Kim HJ, Cho KO, Shin SS, Youn HJ, Hwang CY, Youn HY. An autochthonous case of canine visceral leishmaniasis in Korea. Korean J Parasitol 2013; 51: 545-549.   DOI
14 Solano-Gallego L, Morell P, Arboix M, Alberola J, Ferrer L. Prevalence of Leishmania infantum infection in dogs living in an area of canine leishmaniasis endemicity using PCR on several tissues and serology. J Clin Microbiol 2001; 39: 560-563.   DOI
15 Martin-Sanchez J, Pineda JA, Morillas-Marquez F, Garcia-Garcia JA, Acedo C, Macias J. Detection of Leishmania infantum kinetoplast DNA in peripheral blood from asymptomatic individuals at risk for parenterally transmitted infections: relationship between polymerase chain reaction results and other Leishmania infection markers. Am J Trop Med Hyg 2004; 70: 545-548.
16 Scarlata F, Vitale F, Saporito L, Reale S, Vecchi VL, Giordano S, Infurnari L, Occhipinti F, Titone L. Asymptomatic Leishmania infantum/chagasi infection in blood donors of western Sicily. Tran R Soc Trop Med Hyg 2008; 102: 394-396.   DOI
17 Fukutani KF, Figueiredo V, Celes FS, Cristal JR, Barral A, Barral-Netto M, de Oliveira CI. Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil. BMC Infect Dis 2014; 14: 422.   DOI
18 Hamarsheh O, Nasereddin A, Damaj S, Sawalha S, Al-Jawabreh H, Azmi K, Amro A, Ereqat S, Abdeen Z, Al-Jawabreh A. Serological and molecular survey of Leishmania parasites in apparently healthy dogs in the West Bank, Palestine. Parasit Vectors 2012; 5:183.   DOI
19 Otranto D, Paradies P, de Caprariis D, Stanneck D, Testini G, Grimm F, Deplazes P, Capelli G. Toward diagnosing Leishmania infantum infection in asymptomatic dogs in an area where leishmaniasis is endemic. Clin Vaccine Immunol 2009; 16: 337-343.   DOI
20 Ferreira Ede C, de Lana M, Carneiro M, Reis AB, Paes DV, da Silva ES, Schallig H, Gontijo CM. Comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations. Vet Parasitol 2007; 146: 235-241.   DOI
21 Moreno EC, Goncalves AV, Chaves AV, Melo MN, Lambertucci JR, Andrade AS, Negrao-Correa D, de Figueiredo Antunes CM, Carneiro M. Inaccuracy of enzyme-linked immunosorbent assay using soluble and recombinant antigens to detect asymptomatic infection by Leishmania infantum. PLoS Negl Trop Dis 2009; 3: e536.   DOI
22 Porrozzi R, Santos da Costa MV, Teva A, Falqueto A, Ferreira AL, dos Santos CD, Fernandes AP, Gazzinelli RT, Campos-Neto A, Grimadi G Jr. Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs. Clin Vaccine Immunol 2007; 14: 544-548.   DOI
23 Silva LA, Romero HD, Fagundes A, Nehme N, Fernandes O, Rodrigues V, Costa RT, Prata A. Use of the polymerase chain reaction for the diagnosis of asymptomatic Leishmania infection in a visceral leishmaniasis-endemic area. Rev Inst Med Trop Sao Paulo 2013; 55: 101-104.   DOI
24 Costa CH, Gomes RB, Silva MR, Garcez LM, Ramos PK, Santos RS, Shaw JJ, David JR, Maguire JH. Competence of human host as a reservoir for Leishmania chagasi. J Infect Dis 2000; 182: 997-1000.   DOI
25 Lun ZR, Fang Y, Wang CJ, Brun R. Trypanosomiasis of domestic animals in China. Parasitol Today 1993; 9: 41-45.   DOI
26 Lachaud L, Marchergui-Hammami S, Chabbert E, Dereure J, Dedet JP, Bastien P. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J Clin Microbiol 2002; 40: 210-215.   DOI
27 Costa CH, Stewart JM, Gomes RB, Garcez LM, Ramos PK, Bozza M, Satoskar A, Dissanayake S, Santos RS, Silva MR, Shaw JJ, David JR, Maguire JH. A symptomatic human carriers of Leishmania chagasi. Am J Trop Med Hyg 2002; 66: 334-337.   DOI