• Korean Journal of Poultry Science
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• v.38 no.3
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• pp.191-195
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• 2011

The objectives of this study are to identify specific functional genes which are related with growth and protein structure of the pectoral muscle of Korean native chicken. Pectoral muscle was isolated from three Korean native chickens (KNC, red brown, 12 months old, 2.41 ${\pm}$ 0.24 kg) and three Cornish chickens (16 month old, 2.76 ${\pm}$ 3.0 kg). The subtraction cDNA library was prepared in PCR4 Blunt-TOPO vector. The DNA sequence homology was compared with other breeds and species in GenBank. A clone NDS-81 was found to be unique for the DNA sequence homology with UBX family. Their partial sequence has high homology (98%) with chicken UBX domain D. Chicken UBX domain has chicken (93%), cattle (68%), dog (67%), mouse (64%) and, human (63%) nucleotide sequence homology. Several regions were mutated from T in chicken to C or G in the NDS-81 clone. The first site is LAD in chicken, but it was expressed as (L)RM in clone NDS-81. In this site, amino acids were changed from Ala to Arg, and from Asp to Met. The second site was changed from ER (Arg) in chicken to ED (Asp) in clone NDS-81. They are both containing functional side chains and play an important role in binding other proteins. Therefore, the clone NDS-81 could be a different candidate gene for the UBX family gene and could related with pectoral muscle structure of Korean native chicken.

• Journal of the Korea Computer Graphics Society
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• v.2 no.2
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• pp.61-68
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• 1996

In this paper we propose a new visualization technique to characterize qualitative information of a large DNA sequence. While a long DNA sequence has huge information, it is not easy to obtain genetic information from the DNA sequence. We transform DNA sequences into a polygon to compute their homology in image domain rather than text domain. Our program visualizes DNA sequences with colored random walk plots and simplify them k-convex hulls. A random walk plot represents DNA sequence as a curve in a plane. A k-convex hull simplifies a random work plot by removing some parts of its insignificant information. This technique gives a biologist an insight to detect and classify DNA sequences with easy. Experiments with real genome data proves our approach gives a good visual forms for long DNA sequences for homology analysis.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1022-1030
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• 2004

The gene encoding Aquifex pyrophilus (Apy) DNA polymerase was cloned and sequenced. The Apy DNA polymerase gene consists of 1,725 bp coding for a protein with 574 amino acid residues. The deduced amino acid sequence of Apy DNA. polymerase showed a high sequence homology to Escherichia coli DNA polymerase I-like DNA polymerases. It was deduced by amino acid sequence alignment that Apy DNA polymerase, like the Klenow fragment, has only the two domains, the $3'{\rightarrow}5'$ exonuclease domain and the $5'{\rightarrow}3'$ polymerase domain, containing the characteristic motifs. The Apy DNA polymerase gene was expressed under the control of T7lac promoter on the expression vector pET-22b(+) in E. coli. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $UNO^{TM}$ Q column chromatographies. The optimum pH of the purified enzyme was 7.5, and the optimal concentrations of KCl and $Mg^{2+}$ were 20 mM and 3 mM, respectively. Apy DNA polymerase contained a double strand-dependent $3'{\rightarrow}5'$ proofreading exonuclease activity, but lacked any detectable $5'{\rightarrow}3'$ exonuclease activity, which is consistent with its amino acid sequence. The somewhat lower thermostability of Apy DNA polymerase than the growth temperature of A. pyrophilus was analyzed by the comparison of amino acid composition and pressure effect.

• Bulletin of the Korean Mathematical Society
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• v.55 no.1
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• pp.1-8
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• 2018

In this paper, we study some topological structure of a compact domain in ${\mathbb{R}}^n$ in terms of the curvature conditions and develop a geometric inequality involving the volume and the integral of mean curvatures over the boundary of the compact domain.

• Annual Conference on Human and Language Technology
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• 2020.10a
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• pp.569-571
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• 2020

시맨틱 파싱은 주어진 자연어 발화를 domain specific language(DSL)를 따르는 프로그램으로 변환하는 방법이다. 시맨틱 파서가 다형성을 가지는 DSL을 사용할 경우, 적은 수의 토큰으로 다양한 프로그램을 출력할 수 있지만, 탐색 공간이 넓어진다는 문제가 있다. 본 연구에서는 해당 문제를 완화하기 위해 다형성을 가지는 DSL의 타입 시그니처를 제한하는 방법을 제안한다. 해당 방법은 sequence-to-sequence 기반의 시맨틱 파싱 성능을 향상시키는데 효율적임을 보였다.

• KIISE Transactions on Computing Practices
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• v.21 no.11
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• pp.713-720
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• 2015

As computing and network technologies are further developed, communication equipment continues to become smaller, and as a result, mobility is now a predominant feature of current technology. Therefore, demand for speech recognition systems in mobile environments is rapidly increasing. This paper proposes a novel method to recognize the Korean vowel 'ㅐ' as a part of a phoneme-based Korean speech recognition system. The proposed method works by analyzing a sequence of patterns in the time domain instead of the frequency domain, and consequently, its use can markedly reduce computational costs. Three algorithms are presented to detect typical sequence patterns of 'ㅐ', and these are combined to produce the final decision. The results of the experiment show that the proposed method has an accuracy of 89.1% in recognizing the vowel 'ㅐ'.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.314-322
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• 2021

Paired box protein, PAX7, is a key molecule for the specification, maintenance and skeletal muscle regeneration of muscle satellite cells. In this study, we identified and characterized the cDNA and amino acid sequences of PAX7 from black sea bream (Acanthopagrus schlegelii) via molecular cloning and sequence analysis. A. schlegelii PAX7 cDNA was comprised of 1,524 bp encoding 507 amino acids and multiple sequence alignment analysis of the translated amino acids showed that it contained three domains including paired DNA-binding domain, homeobox domain and OAR domain which were well conserved across various animal species investigated. Pairwise Sequence Alignment indicated that A. schlegelii PAX7 had the same amino acid sequences with that of yellowfin seabream (A. latus) and 99.8% identity and similarity with that of gilt-head bream (Sparus aurata). Molecular phylogenetic analysis confirmed that A. schlegelii PAX7 formed a monophyletic group with those of teleost and most closely related with those of the fish that belong to Sparidae family including A. latus and S. aurata. In the investigation of its tissue specific mRNA expression, the expression was specifically identified in skeletal muscle tissue and a weak expression was also shown in gonad tissue. The cultured cells derived from skeletal muscle tissues expressed PAX7 mRNA at early passage but the expression was not observed after several times of subculture.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.64-64
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• 2003

The sex differentiation of fishes occurs under the control of genetic and various environmental factors. DM-domain containing genes are novel zinc finger transcription factors and play key roles in sex determination. In order to isolate the wrasse DMRT (wDMRT) cDNA from the protogynous wrasse (Halichoeres tenuispinnis), the wrasse testis cDNA library was screened using the $^{32}$ P-labeled PCR products, which were amplified with the degenerate primers from conserved DM-domain regions of several DMRT genes. Among a few positives obtained through screening, the full length wDMRT cDNA of 2.9kb size encoding a predicted 300 amino acid residues was isolated. The sequence analysis exhibited 60%, 43% sequence identity with rainbow trout and tilapia DMRT1, respectively. RT-PCR assay showed that wDMRT was expressed specifically in male testis. Also, wDMRT gene was strongly expressed in May during reproductive season, when the reproductivity of wrasse is most active. This results suggested that wDMRT gene function in testis differentiation The conserved DM-domain regions were amplified using PCR from DMRT genes of several species among Labridae, and their sequences were determined. The sequence of DM-domain region of Halichoeres. tenuispinis was identical to those of Pseudolabrus japonicus, Pteragogus flagellifera, and showed 94% identity with that of Halichoeres poecioptrerus.

• ETRI Journal
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• v.35 no.5
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• pp.739-747
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• 2013

This paper investigates the multifarious nature of the long-term evolution (LTE) scheme and that of the modified LTE scheme for symbol timing synchronization (STS). This investigation allows us to propose a new replica correlation-based STS scheme to overcome the inherent weaknesses of the other two schemes. The proposed STS signal combines a gold sequence and a half sine wave in the time domain, whereas conventional STS signals specify either binary sequences or complex sequences in the time domain or in the frequency domain. In the proposed scheme, a sufficient correlation property is realized by the gold sequence, and robustness against the frequency offset (FO) is achieved through the sine wave. Compared to the existing LTE-related schemes, the proposed scheme can better achieve immunity to FO and reduction in detector complexity, as well as a low peak-to-average power ratio and a low detection error rate. Performance evaluations through analysis and simulation are provided in the paper to demonstrate these attributes.

• Journal of Plant Biology
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• v.38 no.4
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• pp.359-364
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• 1995

As the first step to elucidate the relationship between the structure and function of CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) in plants, the partial nucleotide sequence of soybean cytidylyltransferase cDNA was determined using a polymerase chain reaction (PCR). Degenerate oligonucleotide primers were synthesized from the conserved region revealed from the rat and yeast cytidylyltransferase DNA sequences. The catalytic domain region showed 78 and 76% homology with the rat and yeast amino acid sequences, respectivly. The hydropathy profile indicated that the C-terminal non-catalytic portion of the protein was very hydrophilic, and in the region between the catalytic domain and the C-terminal region, there was a large amphipathic $\alpha$-helical domain that was believed to bind the membrane surface in the active formation. There are 7 potential sites for phosphorylation by protein kinase C and 4 potential sites for phosphorylation by Ca2+/calmodulin kinase within the determined sequence.