• Journal of Plant Biotechnology
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• 제49권4호
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• pp.347-355
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• 2022

제주 용암해수에 함유된 풍부한 미네랄은 노화를 유발하는 활성산소를 제거하는 항산화 효과가 있는 것으로 알려져 있다. 이에 용암해수는 기능성 식품소재로서 상업적 가치가 높은 것으로 알려져 있다. 본 연구에서는 제주산 작물(당근, 블루베리, 감귤)추출물의 항산화 활성을 증류수 추출물과 용암해수 추출물을 이용하여 비교 분석하였다. 블루베리와 감귤의 총폴리페놀 및 플라보노이드 함량은 증류수 추출물보다 용암해수 추출물에서 더 높았다. 또한, 이들 작물의 항산화 효소 활성과 DPPH 라디칼 소거 활성은 증류수 추출물보다 용암해수 추출물에서 더 높았다. 특히, Vero 세포 이용한 블루베리와 감귤의 ROS 소거 효능은 용암해수 추출물에서 더 높게 나타났다. 한편, 당근의 항산화 활성은 총 폴리페놀과 플라보노이드 함량에 차이가 없었음에도 불구하고 용암해수 추출물에서 더 높았다. 이러한 결과는 용암해수가 기능성 식품 산업에서 용매로서의 가능성이 있음을 시사하였으며, 용암해수를 이용한 제주산 작물 추출물은 기능성 식품의 원료로 사용될 수 있는 것으로 판단된다.

• 대한본초학회지
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• 제23권1호
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• pp.93-101
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• 2008

Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of the water and ethanol extracts from medicinal herbs. Methods : Three kinds of medicinal herbs(Corni fructus, Astragalus membranaceus and Glycyrrhiza uralensis) were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidative and anticancer activities. Results : The highest polyphenol contents of the water and ethanol extracts from medicinal herbs were 342.14 mg and 435.62 mg per 100 g of Cornus officinalis, respectively. The highest electron donating abilities (EDA) of the water and ethanol extracts from Glycyrrhiza uralensis were 88% and 91% at 1,000 ppm, respectively. The water and ethanol extracts from Astragalus membranaceus had the highest nitrite scavenging abilities (NSA) at 1,000 ppm. The highest anticancer activity of the extracts were from Glycyrrhiza uralensis against both of MDA and A549 cells. Conclusions : These results suggest that the medicinal herbs can be used as natural antioxidant to prevent oxidative damage in normal cells probably because of their antioxidant characteristics.

• 대한본초학회지
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• 제24권3호
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• pp.51-58
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• 2009

Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of the water and ethanol extracts from pickled soybean products by manufacturing stage. Methods : Yakkong (YK) was pickled for 15 days to prepare Chokong (CK) and Chokong pills (CKP) were mixed Chokong (CK) powder with vinegar. Herbal Chokong pills (HCKP) were made by addition five kinds of medicinal herbs to Chokong. Pickled soybean products by each manufacturing process were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidative and anticancer activities. Results : The highest electron donating abilities (EDA) of the water and ethanol extracts were from HCKP among pickled soybean products and those were 81.2% and 91.5% at 1,000 $\mu$g/mL, respectively. The ethanol extracts of pickled soybean products had higher activities of EDA and superoxide dismutase (SOD) than water extracts. The highest anticancer activity was the water extracts of HCKP against both of MDA and A549 cells. Conclusions : These results suggest that herbal Chokong pills (HCKP) can be used as natural antioxidant to prevent oxidative damage in normal cells probably because of their antioxidative characteristics.

• 한국염색가공학회지
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• 제29권4호
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• pp.256-267
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• 2017

The purpose of this study was to analyze the functional ingredients of Perilla Frutescens L. Britt extracts and to confirm the possibility of producing PVA nanofibers using extracts. Distilled water, 3% aqueous sodium hydroxide solution and ethanol were used as extraction solvents. The electrospinning was carried out at a PVA concentration of 12%, an applied voltage of 10 kV and a tip to collector distance of 15cm. The contents of volatile substances, essential oils, total polyphenols and flavonoids of the extracts were measured to examine the constituents of functional materials. Flavor components and esters were identified in 3% sodium hydroxide and ethanol extracts. The content of polyphenols and flavonoids in ethanol extracts was higher than that of medicinal plants. 1wt.% of Tween 20 was added to disperse the essential oil components of the ethanol extract. Addition of a dispersant made it possible to produce a homogeneous mixture by having some compatibility with the ethanol extracts and the PVA molecule. When the concentration of the ethanol extract was 0.25 and 0.5wt%, relatively uniform PVA nanofiber having an average diameter of 350 to 365nm could be produced. The results of FT-IR, XRD and DSC analysis confirmed that Perilla Frutescens L. Britt ethanol extract was well mixed with PVA molecules and was electrospun.

• 한국식품조리과학회지
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• 제24권1호
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• pp.52-58
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• 2008

The purpose of this research relates to the development of natural preservatives. Here, Samultang ingredients (Rehmannia glutinosa, Cnidium officinale, Paeonia lactiflora and Angelica gigas) were extracted with distilled water and 70% ethanol, and the antibacterial and antioxidative activities of the extracts were tested. The highest polyphenol contents were found in the water and ethanol extracts of Paeonia lactiflora (100 g) at 843.2 mg and 721.1 mg, respectively (p<0.05). The ethanol extract of Cnidium officinale demonstrated antibacterial activity against L. monocytogenes and S. aureus, and that from Paeonia lactiflora against S. aureus. The electron donating abilities (EDA) of the water extracts ranged from 67 to 84%, and those of the ethanol extracts ranged from 68 to 84% at 1,000 ppm. The superoxide dismutase (SOD)-like activities of the water extracts ranged from 47 to 50%, and those of the ethanol extracts ranged from 50 to 56% at 1,000 ppm. The nitrite scavenging abilities (NSA) of the water extracts at 1,000 ppm of the water extracts ranged from 30 to 49%, and those of the ethanol extracts ranged from 42 to 54% at pH 1.2, respectively. The NSAs of the extracts were highest at pH 1.2, and then decreased with increasing pH. The highest levels of antibacterial and antioxidant activity occurred with the water and ethanol extracts of Paeonia lactiflora, which had the highest polyphenol content among the Samultang ingredients. Over all, the ethanol extracts of the Samultang ingredients had higher activities than the water extracts. Considering the combined results, one can conclude that Samultang ingredient extracts would be useful as natural preservatives in the development of health foods.

• 한국융합학회논문지
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• 제8권12호
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• pp.425-430
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• 2017

본 연구는 토복령 추출물의 여드름 균에 대한 항균활성 및 방부효과를 측정하여 최근 논란이 되고 있는 합성방부제의 대체 가능한 천연 항균 추출물의 개발을 위해 연구하였다. 토복령은 국내산으로 건조한 후 증류수와 95% EeOH 두 가지 용매를 각각 사용하여 실온에서 추출하였다. 그 결과 2종 P ropionibacterium acnes 3314, Propionibacterium acnes 3320의 여드름 균에 대한 항균 효과가 매우 우수하였으며, 특히 증류수 추출물의 clear zone이 16.61 mm로 가장 높은 활성을 나타내었다. 또 피부오염 군집을 채취한 결과에서는 24.48 mm의 clear zone을 보여 토복령 추출물은 여드름균에 대한 항균 활성뿐 아니라 그 외 피부 상재균에 대한 항균 활성도 높음을 알 수 있었다. 이 상의 결과로 토복령 추출물은 항균효능 및 방부 효능을 갖는 화장품 원료로서의 가능을 확인할 수 있었고, 향후 천연 방부제 개발을 위한 기초 연구 자료가 될 것으로 사료된다.

• 한국응용과학기술학회지
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• 제8권1호
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• pp.79-83
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• 1991

The extracts of Quercisemen(Quereus acutissima Carruthers seed) powder was obtained by the extraction with distilled water, water : acetonet(1:1, v/v), ethanol and ethyl ether, respectively. As a result this experiment, the antioxidative activity of each solvent extracts on linoleic acid were examined as follows: 1. Each fraction extracted by the acetone : water(1:1, v/v), water and ethanol respectively showed high antioxidative activity. 2. A fraction of the tannin extracted from the solvent, acetone, water(1:1, v/v) showed even more antioxidative activity than that of the ${\alpha}$-tocopherol or BHT. 3. Acceleration of peroxide reaction by $Fe^{++}$ and $Cu^{++}$ on the linoleic acid was strongly inhibited by adding the tannin, 4. Organic acid, such as malic acid, citiric acid and tartaric acid with the tannin were showed the synergistic effect fo the antioxidation reaction.

• 대한본초학회지
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• 제23권3호
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• pp.41-51
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• 2008

Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of extracts from Scutellaria baicalensis, Zizyphus jujuba and Atractylodes macrocephala. Methods : Three kinds of medicinal herbs were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidant activity and growth inhibition activity against cancer cells. Results : Electron donating abilities (EDA) of the water and ethanol extract from Zizyphus jujuba and Atractylodes macrocephala were over than 87% at 1,000 ${\mu}g/mL$, respectively. The highest nitrite scavenging abilities (NSA) of the water and ethanol extracts from Scutellaria baicalensis were 68.9 and 79.2% at 1,000 ${\mu}g/mL$, respectively. Atractylodes macrocephala extracts had shown the highest growth inhibition activity against both of MDA and A549 cells and the water extract had higher activity than ethanol extract. Conclusions : These results suggest that the extracts of medicinal herbs used for this experiment had specific biological activity and they can be used as natural antioxidant to prevent oxidative damage in normal cells.

• 한국식품영양과학회지
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• 제24권6호
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• pp.948-955
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• 1995

겨자의 항균성을 조사하기 의하여 추출용매에 따른 겨자의 항균성을 비교하고, 항균물질을 동정한 결과물, 메탄올 및 헥산에 대한 겨자 추출물의 항균성은 물 및 메탄올 추출물이 실험균주 모두에서 나타났으나 헥산에 의한 겨자 추출물은 항균활성이 거의 나타나지 않았다. SDE장치를 이용한 겨자 추출물의 항균성은 증류성분에서 항균활성이 아주 크게 나타났고, 메탄올에 의한 잔사 추출물도 항균활성이 조금 나타났으나 물층은 항균활성이 거의 나타나지 않았다. 겨자로 부터 3-iso-thiocynate-1-propene, 4-isothiocyanato-1-butene 및 4-ios-thiocyanatoethyl benzene과 같은 3종류의 isothiocyanate를 포함하여 30여종의 증류성분이 동정되었다.

• 대한한방내과학회지
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• 제22권2호
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• pp.193-198
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• 2001

This study was designed to investigate the effects of Korean Lycii Fructus water extract in Pb-administered rats. The Pb exposed rats were given 100 ppm and 200 ppm in the distilled water. Sixty male Sprague-Dawley rats weighing between 90 and 110g were blocked into 6 groups according to body weight. The control group was fed a normal diet, without lead. The experimental groups, which was fed a normal diet plus 100 ppm and 200 ppm lead, and one group received a normal diet plus Lycii Fructus water extracts. The results: the Food intake, the weight gain, and the kidney weight content in the cadmium added groups were lower than those in the Lycii Fructus water extracts group. The contents of Pb in the kidneys of the rats were determined by using ICP(lnductively Coupled Plasma Spectrophotometer). The accumulation of lead in the kidney was lower in the Lycii Fructus water extracts group. The Plasma levels of renin activity was higher in the lead administration groups, as compared with the Lycii Fructus water extracts. Plasma levels of aldosterone activity was higher in the lead administration group, as compared with Lycii Fructus water extracts. These results suggest that Lycii Fructus water extracts has a lowering effects on the accumulation of pb on kidney and it is believed that the Lycii Fructus water extracts have some protective effects on lead-induced nephrotoxicity in rats, but the mechanism of these effects was obscure.