• Title/Summary/Keyword: distilled water extracts

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Optimal Extraction Conditions of Anti-obesity Lipase Inhibitor from Phellinus linteus and Nutritional Characteristics of the Extracts

  • Lee, Jong-Kug;Song, Jung-Hwa;Lee, Jong-Soo
    • Mycobiology
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    • v.38 no.1
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    • pp.58-61
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    • 2010
  • In an effort to develop novel mushroom-derived anti-obesity nutraceuticals, water and ethanol extracts containing the lipaseinhibitory compound from Phellinus linteus were prepared, and their nutritional components were determined. The optimal conditions for the extraction of P. linteus lipase inhibitor involved the treatment of the fruiting bodies with distilled water at $80^{\circ}C$ for 72 hr and 80% ethanol at $100^{\circ}C$ for 60 hr, respectively. The distilled water extract and ethanol extract contained 10.9% and 6.11% of crude protein, and 0.96% and 15.86% of crude fat, respectively. Additionally, the distilled water extract contained a large quantity of minerals, including 239.5 mg of K, 39.3 mg of Mg, and 39.3 mg of Na. The free amino acid content of the distilled water extracts was also higher than that of the ethanol extracts, and in particular, the distilled water extracts contained 5,139 mg of asparagine, 3,891 mg of tryptophan, 2,598 mg of alanine, and 2,066 mg of serine in 100 g of the distilled water extracts. 100 g of the distilled water and ethanol extracts were found to contain 12.31 g and 8.16 g of malic acid, respectively.

Characteristics and Dyeability of Juniperus Chinensis Extracts (향나무 추출 색소의 특성 및 염색성)

  • Nam, Ki Yeon;Lee, Jung Soon
    • Korean Journal of Human Ecology
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    • v.21 no.5
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    • pp.989-1004
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    • 2012
  • The purpose of this study was to investigate the characteristics of the pigment and the dyeability of juniperus chinensis needles, berry, bark and heartwood extracts using distilled water, methanol, normal butanol and ethyl acetate as a solvent. Ultraviolet-visible absorption spectrum bands of the extracts were measured at around 280nm to 320nm in all the solvent extracts. The maximum absorption wavelength was able to determine tannin. All the solvent extracts except for distilled water extracts were able to confirm the presence of chlorophyll. Infrared absorption spectra (FT-IR) of all parts of the extracts showed broad absorption bands of OH due to phenolic-OH, benzene CH peak of phenol chemical structure, ether-based stretching vibration peak and the peak of flavonoid compounds that appeared in all the solvent extracts. The yield of juniper needles and heartwood in distilled water and methanol extraction were effective. Extraction of berry yields in distilled water was also effective. The yield of ethanol extraction from the bark showed better efficiency. As a result of using distilled water for the dye solution, the color of the fabrics dyed with all extracts of the needles and Y series berries generally showed light Y progression with a strong red tinge. By using a mixture of 20% ethanol and 80% distilled water for the dye solution, the color of the fabrics dyed with needles and berry extracts showed Y series dominantly. The color of the fabrics dyed with Bark and heartwood extracts were dominantly R series.

Antioxidant activities of soymilk added with green tea and rosemary extract (녹차와 로즈마리 추출물을 첨가한 두유의 항산화 활성)

  • Ryu, Ji-yeon;Song, YeonWoo;Moon, Jeong Yong;Jun, Neung-Jae;Kim Cho, Somi
    • Food Science and Preservation
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    • v.24 no.6
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    • pp.871-878
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    • 2017
  • The objective of this study was to compare antioxidant activities of green tea, rosemary, lemon and bamboo leaves extracts using three different extraction methods (80% ethanol sonication extraction, distilled water autoclave extraction, distilled water extraction at room temperature). As a result, green tea and rosemary extracts showed relatively high antioxidant activities compared with those of lemon and bamboo leaves. These green tea and rosemary extracts were subsequently added to soymilk and their mixtures were examined for antioxidant activities. Soymilk added with 10% green tea distilled water autoclave extracts and distilled water at room temperature extracts showed relatively high DPPH, $85.09{\pm}2.26%$, $84.38{\pm}1.97%$, and ABTS radical scavenging activities, $73.43{\pm}2.78%$, $81.34{\pm}4.78%$, respectively. On the other hand, soymilk added with 10% rosemary distilled water autoclave extracts showed remarkably higher DPPH radical scavenging activity while their ABTS radical scavenging ability was similar to that of non-added soymilk. In conclusion, our results suggest that the addition of green tea distilled water autoclave extracts, distilled water at room temperature extracts and rosemary distilled water autoclave extracts to soymilk could contribute to the development of added value soymilk products with increased antioxidant activities.

Cell Proliferation and Antioxidative Effects of Ultrasonic Coffee Extracts

  • Jin, Hyunwoo
    • Biomedical Science Letters
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    • v.23 no.4
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    • pp.388-394
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    • 2017
  • Recently, coffee is the most popular beverage for modern people. A great number of substances are found in coffee beans and have been studied for many years such as aliphatic and aromatic compounds. However, studies on the physiological activity of coffee extracts are insufficient. This study was performed to determine the contents of caffeine and chlorogenic acid in coffee extracts according to the solvent and to investigate the physiological activity of coffee extracts. Coffee extracts were extracted by ultrasonication method with various types of solvents including distilled water, ethanol, and other organic solvents under $50^{\circ}C$ and $80^{\circ}C$. The contents of caffeine and chlorogenic acid in coffee extracts were determined by Liquid Chromatography-Mass Spectrometry (LC-MS). Also, cytotoxic and antioxidative effects of coffee extracts were evaluated with MTT and DPPH assays to analyze the physiological activity. As a result, it was confirmed that caffeine and chlorogenic acid contents were extracted in distilled water with the highest rate. Antioxidative activity was observed below 10-fold dilute of coffee extracts, however cytotoxicity was not observed. In conclusion, distilled water was the best solvent for extracting caffeine and chlorogenic acids from coffee bean with ultrasonication and these coffee extracts are less cytotoxic in human skin cell lines and have antioxidant effect.

The Effect of Vegetable Extracts on the Activity of Alcohol Dehydrogenase from Saccharomyces cerevisiae

  • Jung, Soon-Teck;Kang, Bae-Kwang
    • Preventive Nutrition and Food Science
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    • v.6 no.4
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    • pp.224-229
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    • 2001
  • We investigated the effects of bean sprouts (Glycine max), dropwort (Oenanthe javanica), and radish (Raphanus sativus var. hortensis for. acanthiformis) extracts on alcohol dehydrogenase (ADH). The extracts from three kinds of vegetables were prepared by extracting with boiling water, distilling water, and ethyl alcohol. Among extracts, boiling water extract showed the highest activating effect on ADH, respectively and distilled water extract had a greater effect on ADH activation than that of alcohol extract. The ADH facilitating effect of bean sprout extract by distilled water was significantly higher than dropwort or radish, hut the effect of the bean sprout extract by ethyl alcohol was lower than others. The facilitating effect on ADH of mixture extracts of bean sprout and dropwort were mixed at 1 : 1 mixture of boiled-water extract showed the highest effectiveness. And bean sprout extract separated below 3000 molecular weight (MW) range of extract fraction had greater ADH activity than large MW parts.

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Fabrication of Electrospun PVA Nanofibers Loaded with Artemisia capillaris Thunberg Extracts (인진쑥 추출물 함유 PVA 나노섬유 제조)

  • Kim, Myoung Ok;Lee, Jung-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.42 no.2
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    • pp.269-277
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    • 2018
  • This study extracted Artemisia capillaris Thunberg with distilled water and ethanol to investigate its antioxidant effect. We then investigated the possibility of producing nanofibers by an electrospinning process by adding the extracts to polyvinyl alcohol (PVA). The electrospinning method used a PVA concentration of 12wt.%, an applied voltage of 10kV, and a tip-to-collector distance of 15cm. Total polyphenol and total flavonoid contents were measured to verify the antioxidant activity of Artemisia capillaris Thunberg extracts (ACEs). The total polyphenol content of the distilled water extract and the ethanol extract were measured as 218.47 and 271.26mg/g, respectively, and the total flavonoid content of the distilled water extract and the ethanol extract were measured as 141.68 and 34.98mg/g, respectively. As the content of the ACEs in the PVA nanofibers increased, the Diameters of the nanofibers and the uniformity of the diameters decreased. The electrospinning process was fabricated in a relatively uniform form without beads, and the diameters of the nanofibers that were produced ranged from 340 to 390nm. The results of FT-IR, XRD and DSC analyses confirmed that the ACEs were well mixed with the PVA molecules and were electrospun.

A Study on the Functional Improvement of Natural Dyed Fabrics (천연염색 직물의 기능성 향상에 관한 연구)

  • Seo, Myoung-Hee
    • The Korean Journal of Community Living Science
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    • v.19 no.2
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    • pp.213-221
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    • 2008
  • In this study, a dye bath was made using a mixture of distilled water, pine needle extract and bamboo vinegar. Analysis and comparison of various functions of cotton fabrics dyed using bamboo charcoal and gardenia seeds in distilled water and in the dye bath mixture were done. The summary of the results is as follows. Comparing bamboo vinegar and the pine needle extracts, the bamboo vinegar had a higher antibacterial function. In the case of natural dyeing cotton fabrics using bamboo charcoal and gardenia seeds, the additional amount of dyestuff for optimization is 10 g and 20 g respectively per 1 liter of distilled water. The suitable dye bath mixture ratio of distilled water, pine needle extract, and bamboo vinegar considering functionality and economical efficiency of dyed fabrics is 4:3:3. By using the mixture made in this study as a dye bath, improved antibacterial function, deodorization, and colorfastness can be obtained more than distilled water alone.

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Characteristics and Dyeability of Perilla Frutescens L. Britt Extracts with Different Solvents (추출용매에 따른 자소 색소의 염색성 및 기능성)

  • Wang, Qian Wen;Lee, Jung-Soon
    • Textile Coloration and Finishing
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    • v.28 no.3
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    • pp.195-207
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    • 2016
  • In this study, we examined the influence of the pigment characteristic and dyeing condition on dyeing properties and functionality by using Perilla Frutescens L. Britt extracts, in which ethanol, distilled water and NaOH solution were used as 3 different solvents. Changes in dyeing conditions include variations in dye concentration, dyeing temperature, time and pH on dye uptake, and K/S values were compared according to these changes. Additionally, color changes were observed according to the use and types of mordant. Ultraviolet-visible spectrum was utilized to investigate the pigment characteristic, and as a result, chlorophyll was identified in ethanol extract, whereas tannin was identified both in distilled water extract and NaOH solution extract. By using FT-IR analysis, these tannins in distilled-water-extract and NaOH solution extract were verified to be hydrolyzable tannin. When dyeing silk, dye uptake increased as dye concentration, dyeing temperature and time increased, while it decreased as pH of the extract increased. Fabrics dyed without a mordant produced Y-series colors, and fabrics dyed with mordants showed various colors depending on the mordant types. Even though color fastness to washing and light was unsatisfactory, fastness to rubbing and perspiration showed relatively high grade. Moreover, deodorant ability of dyed fabric improved.

Analysis of Ginsenosides of Black Ginseng (흑삼의 인삼 사포닌 분석)

  • Han Sung Tai;Whang Wan Kyun;Kim Il Hyuk;Yang Byung Wook;Cho Soon Hyun;Ko Sung Kwon
    • YAKHAK HOEJI
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    • v.49 no.6
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    • pp.490-494
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    • 2005
  • The objective of this study is to provide the basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve such aim, Ginsenoside compositions of black ginseng (BG) extracts with various solvent conditions were examined by HPLC. The total saponin and the prosapogenin content of 95$\%$ ethyl alcohol extract were higher than that of the either 50$\%$ ethyl alcohol extract or distilled water extract. As a result, the order of the total saponin and the prosapogenin content was 1) 95$\%$ ethyl alcohol,2) 50$\%$ ethyl alcohol,3) the first and second mixture of 95$\%$ ethyl alcohol, distilled water, and 4) distilled water extract. In the case of fine black ginseng (FBG), the first and second mixture extracts of 95$\%$ ethyl alcohol and distilled water were the highest. In addition, the ratio of the protopanaxadiol group and the protopanaxatriol group (PD/PT) showed that the ratio of BG ranged from 0.304 to 0.601, while the ratio of FBG ranged from 1.166 to 1.657.

Comparison of Radical Scavenging Activity of Extracts of Mulberry Juice and Cake Prepared from Mulberry (Morus spp.) Fruit

  • Kwon, Yun-Ju;Rhee, Soon-Jae;Chu, Jae-Won;Choi, Sang-Won
    • Preventive Nutrition and Food Science
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    • v.10 no.2
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    • pp.111-117
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    • 2005
  • Radical scavenging activity of water and methanol extracts of mulberry juice and cake prepared from mulberry fruit (Morus spp.) was evaluated using three in vitro assay systems. Mulberry fruits were homogenized with $0.5\%$ trifluoroacetic acid (TFA) in distilled water, filtered with cheeze-cloth and centrifuged to yield mulberry juice and cake. Mulberry juice was evaporated and solubilized in $0.5\%$ TFA in distilled water or $0.5\%$ TFA in $80\%$ aqueous methanol, followed by filtration and evaporation to obtain water (WMJ) and methanol (MMJ) extracts of mulberry juice. Mulberrry cake also was extracted with the above same solvents, and thereby finally obtaining water (WMC) and methanol (MMC) extracts of mulberry cake. Among four extracts, the MMC showed the most potent radical scavenging activity against DPPH radical $(IC_{50}=167.45\;{\mu}g/mL)$, and superoxide $(IC_{50}=36.18\;{\mu}g/mL)$ and hydroxyl radicals $(IC_{50}=467.08\;{\mu}g/mL)$. The WMC also exhibited stronger radical scavenging activity than those of two other mulberry juice extract, WMJ and MMJ. Meanwhile, the MMJ exerted stronger three radical scavenging activity than the WMJ. Total phenolic content of the water and MeOH extracts from mulberry cake was higher than that of the water and MeOH extracts from mulberry juice. Thus, these results suggest that the extracts of mulberry cake with high dietary phenolics may be useful potential source of natural antioxidant as radical scavenger.