Analysis of Ginsenosides of Black Ginseng

흑삼의 인삼 사포닌 분석

  • 한성태 (중앙대학교 인삼산업연구소) ;
  • 황완균 (중앙대학교 약학대학) ;
  • 김일혁 (중앙대학교 약학대학) ;
  • 양병욱 (중앙대학교 인삼산업연구소) ;
  • 조순현 (중앙대학교 인삼산업연구소) ;
  • 고성권 (세명대학교 한방식품영양학과)
  • Published : 2005.12.01

Abstract

The objective of this study is to provide the basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve such aim, Ginsenoside compositions of black ginseng (BG) extracts with various solvent conditions were examined by HPLC. The total saponin and the prosapogenin content of 95$\%$ ethyl alcohol extract were higher than that of the either 50$\%$ ethyl alcohol extract or distilled water extract. As a result, the order of the total saponin and the prosapogenin content was 1) 95$\%$ ethyl alcohol,2) 50$\%$ ethyl alcohol,3) the first and second mixture of 95$\%$ ethyl alcohol, distilled water, and 4) distilled water extract. In the case of fine black ginseng (FBG), the first and second mixture extracts of 95$\%$ ethyl alcohol and distilled water were the highest. In addition, the ratio of the protopanaxadiol group and the protopanaxatriol group (PD/PT) showed that the ratio of BG ranged from 0.304 to 0.601, while the ratio of FBG ranged from 1.166 to 1.657.

Keywords

References

  1. Namba, T. The encyclopedia of wakan-yaku with color pictures (1). Hoikusha, Osaka, p. 5 (1980)
  2. Lee, D. C., Lee, M. O., Kim, C. Y. and Clifford, D. H. : Effect of ether, ethanol and aqueous extracts of ginseng on cardiovascular function in dogs. Can. J. Comp. Med. 45, 182 (1981)
  3. Jie, Y. H., Cammisuli, S. and Baggiolini, M. : Immunomodulatory effects of Panax ginseng C.A. Meyer in the mouse. Agents Actions. 15, 386 (1984) https://doi.org/10.1007/BF01972376
  4. Kim, Y. C., Kim, S. R., Markelonis, G. J. and Oh, T. H. : Ginsenosides $Rb_1$ and $Rg_3$ protect cultured rat cortical cells from glutamate-induced neurodegeneration. J. Neurosci. Res. 53(4), 426 (1998) https://doi.org/10.1002/(SICI)1097-4547(19980815)53:4<426::AID-JNR4>3.0.CO;2-8
  5. Joo, C. N., Koo, J. D., Kim, D. S. and Lee, S. J. : Biochemical studies of ginseng saponins. XI. The effects of ginseng saponins on alcohol dehydrogenase. Hanguk Saenghwa Hakhoe Chi. 10, 109 (1977)
  6. Tahara, M., Kono, H., Mune, S. and Odashima, S. : Action of ginsenosides on tumor cells. Growth inhibition and redifferentiation of neoplasia. Wakan Yaku Gakkaishi. 2, 170 (1985)
  7. Yokozawa, T., Kobayashi, T., Oura, H. and Kawashima, Y. : Studies on the mechanism of the hypoglycemic activity of ginsenoside-$Rb_2$ in streptozotocin-diabetic rats. Chem. Pharm. Bull. 33, 869 (1985) https://doi.org/10.1248/cpb.33.869
  8. Park, J. D. : Recent studies on the chemical constituents of Korean ginseng. Korean J. Ginseng Sci. 20, 389 (1996)
  9. Sanata, S., Kondo, N., Shoji, J., Tanaka, O. and Shibata, S. : Studies on the saponins of ginseng. I. Structure of ginseng-$R_0,\;Rb_1,\;Rb_2$, Rc and Rd. Chem. Pharm. Bull. 22, 421 (1974) https://doi.org/10.1248/cpb.22.421
  10. Kitagawa, I., Taniyama, T., Shibuya, H., Nota, T. and Yoshikawa, M. : Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2) : Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root. Yakugaku Zasshi. 107, 495 (1987)
  11. Keum, Y. S., Park, K. K., Lee, J. M., Chun, K. S., Park, J. R., Lee, S. K, Kwon, R. and Surh, Y. J. : Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett. 150(1), 41 (2000) https://doi.org/10.1016/S0304-3835(99)00369-9
  12. Kim, S. E., Lee, Y. H., Park, J. H. and Lee, S. K. : Ginsenoside-$Rs_3$, a new diol-type ginseng saponin, selectively elevates protein levels of p53 and p21WAF1 leading to induction of apoptosis in SK-HEP-1 cells. Anticancer Res. 19(1A), 487 (1999)
  13. Kim, W. Y., Kim, J. M., Han, S. B., Lee, S. K., Kim, N. D., Park, M. K., Kim, C. K. and Park, J. H. : Steaming of ginseng at high temperature enhances biological activity. J. Nat. Prod. 63(12), 1702 (2000) https://doi.org/10.1021/np990152b
  14. Bao, H. Y., Zhang, J., Yeo, S. J., Myung, C. S., Kim, H. M., Kim, J. M., Park, J. H., Cho, J. S. and Kang, J. S. : Memory enhancing and neuroprotective effects of selected ginsenosides. Arch. Pharm. Res. 28, 335 (2005) https://doi.org/10.1007/BF02977802
  15. Jung, K. Y., Kim, D. S., Oh, S. R., Lee, I. S., Lee, J. J., Park, J. D., Kim, S. I. and Lee, H. K. : Platelet activating factor antagonist activity of ginsenosides. BioI. Pharm. Bull. 21(1), 79 (1998) https://doi.org/10.1248/bpb.21.79
  16. Korea Food and Drug Administration: Code of Korea Food Regulation 2001., Seoul, p. 396 (2001)
  17. Ko, S. K., Lee, K. H., Hong, J. K., Kang, S. A., Sohn, U. D., Im, B. O., Han, S. T., Yang, B. W., Chung, S. H. and Lee, B. Y. : The change of ginsenoside composition in ginseng extract by the vinegar process. Food Sci. & Biotechnol. 14(4), 509 (2005)
  18. Jang, J. G., Lee, K. S., Kwon, D. W. and Oh, H. K. : Chemical compositions of Korean ginseng with special reference to the part of ginseng plant. Korean J. Ginseng Sci. 11, 84 (1987)
  19. Kim, M. W., Ko, S. R., Choi, K. J. and Kim, S. C. : Distribution of saponin in various sections of Panax ginseng root and changes of its contents according to root age. Korea J. Ginseng Sci. 11, 10 (1987)
  20. Kim, S. C., Choi, K. J., Ko, S. R. and Joo, H. K. : Content comparison of proximate compositions, various solvent extracts and saponins in root, leaf and stem of Panax ginseng. Korean J. Ginseng Sci. 11, 118 (1987)