• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.519-523
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• 2002

Soluble polysaccharide (SP) from green layer, Enteromorpba prolifera was extracted 3 times with distilled water at 100$^{\circ}C$ for 2 hrs and fractionated with cetylpyridinium chloride (CPC) and ion exchange chromatography (DEAE Separose CL-6B). The SP amounted to $23.7\%$ of the dry seaweed weight and contained $68.8\%$ carbohydrate. It was mainly constituted of rhamnose, glucose, xylose, sulfate and uronic acid and was fractionated with CPC into three (CPC-S, CPC-PS, CPC-PP) tractions. The major acid fraction CPC-PS accounted for $10.2\%$ of the dry algal weight. CPC-PS was further fractionated on DEAE Sepharose CL-6B into Fr-1 ($8.0\%$), Fr-2 ($35.8\%$), Fr-3 ($23.7\%$) fractions. The Fr-3 fraction contained $2.2\%$ protein, $21.4\%$ sulfate, $15.3\%$ uronic acid, and $72.4\%$ polysacchnrides composed of rhamnose, xylose and glucose. The Fr-2 fraction, which was richer in uronic acid ($17.5\%$) and poorer in sulfate ($19.0\%$) and total sugar ($68.8\%$) than the Fr-3, had a sugar composition close to that of Fr-3. The average molecular weights of Fr-2 and Fr-3 were 510,000 and 830,000 daltons, respectively. Fr-3 turned out to be homogeneous by cellulose acetate electrophoresis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.5
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• pp.345-355
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• 1991

In order to effectively utilize the by-products of sea-food, the utilization of enzyme-modified flounder(Limanda aspera) skin gelatin as an emulsifier was investigated. In the experiment, the gelatin was extracted from the flounder skin with the heat-treatment at $60^{\circ}C$ and in pH 5.0 for 3 hrs with four volumes of distilled water and emulsifiers were enzymatically modified L-leucine alkyl esters$(L-leucine-OC_n$ : n= 2, 4, 6, 8 and 10) to the gelatin$(EMFSG-C_2,\;EMFSG-C_4,\;EMFSG-C_6,\;EMFSG-C_8,\;EMFSG-C_{10})$ for improving the functional properties such as emulsifying activity, emulsifying viscosity, whippability, electric conductivity, critical micelle concentration and interface tension, etc. Also, the functional properties of the L-leucine alkyl ester modified gelatins were compared with those of Tween-60 as reference. Molecular weights of the enzymatically modified flounder skin gelatin(EMFSG) were 20.5kDa. in $EMFSG-C_2.\;19.5 kDa.\;in\;EMFSG-C_4\;and\;16.5kDa.\;in\;EMFSG-C_6,\;EMFSG-C_8$ and $EMFSG-C_{10}$. respectively. Emulsifying activity and emulsifying viscosity in the modified gelatins were risen with increase of carbon number of the introduced L-leucine alkyl esters. Among the modified gelatins, $EMFSG-C_6$ exhibited the highest emulsifying stability and foaming stability, whereas $EMFSG-C_8$ showed the highest whippability. The electric conductivities of the all $EMFSG-C_n$ were linearly risen to critical micelle concentration(CMC) , therefore $EMFSG-C_{10}$ exhibited the lowest CMC value and interface tension, and dense particles in the microscopic observation. In conclusion, the best quality in functional properties was assured on $EMFSG-C_{10}$.

• Journal of Korean Medicine Rehabilitation
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• v.25 no.2
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• pp.15-35
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• 2015

Objectives This study was performed to investigate the effects of Bujasasim-tang ethanol extract (BST) on oxidative stress, inflammation and osteoarthritic rat model. Methods To ensure safety of BST, heavy metal levels were measured and cytotoxicity test was done. In vitro, To evaluate antioxidative effects of BST, total phenolic contents, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity, reactive oxygen species (ROS) levels were measured. Also, to evaluate anti-inflammatory effects of BST treated group, total nitric oxide (NO) and pro-inflammatory cytokines (IL-$1{\beta}$, IL-6, TNF-${\alpha}$) levels were measured in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In vivo, We injected MIA $50{\mu}l$ (60 mg/ml) into knee joints of rats to induce osteoarthritis. Rats were divided into total 3 groups (normal, control, BST treated group, each n=7). Normal group was not treated at all without inducing osteoarthritis and taken normal diet. Control group was induced osteoarthritis by MIA and taken with 2 ml of distilled water once a day for 4 weeks. BST treated group was induced osteoarthritis by MIA and taken BST 2 ml (200 mg/kg/mouse) once a day for 4 weeks. We evaluated dynamic weight bearing with the Incapacitance Test Meter. At the end of experiment, the rats were sacrificed to observe the functions of liver and kidney, changes of WBC, neutrophil, lymphocyte, monocyte levels in blood, to evaluate the levels of pro-inflammatory cytokines, tissue inhibitor of metallopreteinases-1 (TIMP-1), matrix metalloproteinase-9 (MMP-9), prostaglandin $E_2$ ($PGE_2$), leukotriene $B_4$ ($LTB_4$) within serum. We observed change of articular structures by Hematoxylin & Eosin (H&E), safranin-O staining method and measured amount of cartilage by micro CT-arthrography. Statistical analysis was done by unpaired student's t-test with significance level at p<0.05 in SPSS 11.0 for windows. Results 1. Safety of the BST was identified. 2. AST, ALT, BUN, creatinine levels of BST treated group were within normal limit. In vitro, 1. DPPH and ABTS free radical scavenging activities of BST showed dose-dependent increase. 2. ROS production were significantly decreased. 3. Total nitric oxide (NO) and IL-$1{\beta}$ production were decreased. 4. IL-6 and TNF-${\alpha}$ production were significantly decreased. In vivo, 1. Weight bearing ability was significantly increased. 2. WBC, neutrophil, lymphocyte, monocyte levels in blood were decreased. 3. IL-$1{\beta}$ and TNF-${\alpha}$ levels in serum were significantly decreased. and the IL-6 level was decreased. 4. TIMP-1, MMP-9, $LTB_4$, $PGE_2$ levels in serum were significantly decreased. 5. Cartilage volume of BST treated group was significantly increased. Also changes of cartilage, synovial membrane, fibrous tissue were suppressed. Conclusions The results obtained in this study Bujasasim-tang have effects of antioxidative, anti-inflammatory, relieve pain and protection of cartilage. Therefore we expect that Bujasasim-tang is effective treatment for osteoarthritis.

• The Journal of Korean Academy of Prosthodontics
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• v.43 no.5
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• pp.587-598
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• 2005

Statement of problem: Ceramic restorations should be made of porcelain layers of different opacity, shade, and thickness in order to provide a natural appearance. Lithium disilicate glass-ceramic system has superior color reproducibility, because it uses the ceramic ingot which is similar to teeth shade and uses the staining technique and layering technique. However, staining technique has a fault of discoloration. Also, porcelain is divided core and dentin layer, it is not enough to study about the influence of porcelain layer thickness and shade on the shade of ceramic restorations. Purpose: The purpose of this study was to evaluate the influence of porcelain layer thickness and color on the final shade of ceramic restorations. Materials and method: The CIE $L^*a^*b^*$(CIELAB) values of 72 assembled specimens, each consisting of 3 discs (enamel porcelain 0.2 mm/dentin porcelain -1.2, 0.9, 0.7, 0.5 or 0.3 mm/ceramic core -0.3, 0.5, 0.7, 0.9 or 1.2 mm, diameter is 1.0 mm) were evaluated with a spectrophotometer (Model Chromaview 300, Spectron Tech Co, Korea) for the shade A1, A2, A3 and A4. Distilled water (refractive index: 1.7) was used to attain optical contact between the layers. White, white gray, and white brown backgrounds were used to assess the influence of the background on the final shade. And the mean color difference value$({\Delta}E)$ was calculated. Results and conclusion: The results obtained from this study were as follows. 1. There was a significant correlation between the thickness ratio of the ceramic core/dentin porcelain system and $L^*,\;a^*\;and\;b^*$ values when the total thickness of specimen combination was smaller than 1.4 mm(P<0.05). 2. The specimen which the ceramic core thickness was more than 0.7 mm had the best masking effect against background colors. 3. The mean color difference value$({\Delta}E)$ is smaller than 2 $({\Delta}E<2)$ when the ceramic core thickness was larger than 0.7 mm and the total thickness of specimen was more than 1.4 mm.

• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.2
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• pp.284-292
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• 2005

The aim of this study was to identify and quantify the major or detectable monomers released from any of five commercially-available, light-cured pit and fissure sealants with three different light sources : conventional halogen light curing unit, plasma arc light curing unit and LED curing unit. After curing, specimens were immediately immersed in distilled water for different time intervals. The time related release of monomers were analyzed by high performance liquid chromatography(HPLC). Identification and quantitative analysis of monomers were performed by the comparison of the elution time and the absorption peak height of the eluates with those of the authentic sample. The result of this study can be summarized as follows. 1. Standard solution peaks with retention times of 2.3, 3.2, 5.6, 6.5, 10.4 minutes were identified as BPA, TEGDMA, UDMA, Bis-GMA, Bis-DMA, respectively. 2. None of the chromatograms of the tested sealants displayed peaks with the same retention time as that of the standard solution, except for TEGDMA. 3. The highest release rate of TEGDMA was observed during the 12hr period for all samples and declined thereafter. 4. The elution of TEGDMA from curing with Halogen curing unit for 20 second and LED for 10 second was less than that resulting from curing with Plasma arc for 3 second. 5. TEGDMA was detected at much lower levels in eluates from the Pit & Fissure $Sealant^{TM}$ than other sealants. The elution of TEGDMA from the $Helioseal^{(R)}$ F cured with Halogen light curing unit, the $Concise^{TM}$ cured with Plasma arc curing unit and the $Teethmate^{(R)}$ F-1 cured with LED curing unit were higher than other sealants.

• Journal of Dental Rehabilitation and Applied Science
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• v.25 no.2
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• pp.183-190
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• 2009

The aim of this study is to find the condition of forming the favorable nanotubes by anodizing with NaF and $H_3PO_4$. Machined Ti discs were used for anode, and Platinum net was used for cathode. For electrolyte, $H_3PO_4$ and NaF solution were mixed. We controlled voltage, electrolyte concentration, anodizing time and formed nanotubes on Ti discs. After that, these were washed with distilled water for 24 hours and dried in the $40^{\circ}C$ oven for 24 hours. The surface structure of specimens were analyzed. The results were as follows : At 0.5 wt % NaF, according as increasing voltage and anodizing time, early state of nucleating pores were generated. At 1.0 wt % NaF, 20 V, 20 & 25 min, well-formed nanotubes were observed. At 1.0 wt % NaF, 30 V, structure of nanotube became bigger and interconnected. At 2.0 wt % NaF, no nanotubes were formed and it was unrelated with voltage and time. At 1.0 wt % NaF, 20 V, 20 - 25 min, well-ordered nanotubes were generated on Ti discs. For the formation of favorable nanotubes, it is considered that proper parameters such as electrolyte concentration, voltage, anodizing time are necessary according to the kind of electrolytes.

• The Journal of the Korean dental association
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• v.48 no.6
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• pp.469-477
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• 2010

The purpose of this study was to evaluate the effect of two dentin desensitizers and Er,Cr:YSGG laser for dentinal tubule occlusion. Twenty recently extracted single-rooted human teeth were used to obtain root dentinal fragments. The crowns were cut approximately 1mm below the cementum enamel junction(CEJ). A second cut was used to remove the apex of the root. Subsequently, a longitudinal cut was made in order to obtain 2 fragments from each root sample. The cementum from the cervical portion was removed using a high-speed diamond-coated bur in order to expose the dentin. To open dentinal tubules, forty samples were treated with 50% citric acid for 2 min and then rinsed under distilled water for 1 min. These were divided into four groups of ten samples each. The first group served as a control group. In group 2, the samples were irradiated with the Er,Cr:YSGG laser(Waterlase MD, Biolase, USA). In group 3, the samples were treated with Bisblock and ONE-STEP PLUS(Bisco, USA). In group 4, the samples were treated with Gluma comfort bond & Desensitizer(Heraeus Kulzer, Germany). All the samples were examined using Scanning electron microscopy(Hitachi, S-4700, Japan) with two different magnifications(X2000, X5000). These images were assessed by one examiner who was blind to the experimental procedure, using the index of smear layer removal. The distribution of smear layer removal grades was tested using Fisher's exact test. On the order hand, in order to evaluate the occluding effect of two dentin desensitizers and Er,Cr:YSGG laser, the number of exposed dentinal tubules was counted in each group. These were evaluated using the Kruskal-Wallis test with significance predetermined $\alpha$=0.05. There were statistically significant differences between the three groups(Er,Cr:YSGG laser, Bisblock+ONE-STEP PLUS, Gluma comfort bond & Desensitizer) and control group.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.1
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• pp.7-15
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• 2018

This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

• Restorative Dentistry and Endodontics
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• v.24 no.3
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• pp.511-518
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• 1999

This study was performed to evaluate the mechanical properties of light-cured restorative composites by compression and indentation tests. Five commercially available light-activated composites (Heliomolar : HM, Aelitefil : AF, Amelogen : AG, Clearfil AP-X : CF, Z100 ZH) were used Disc specimens of 12mm in diameter and 1 mm in thickness and Cylindrical specimens of 3mm in diameter and 6mm in length were prepared for the indentation and compressive test, respectively. All specimens were immersed in distilled water at $37^{\circ}C$ for 30 days. An indentation test of 68-degree trigonal diamond pyramid was made under 10 g load for 15 seconds and an Knoop indentation test was made under 50 g load for 15 seconds. Hardness numbers, characteristic indentation depths and permanent deformation were measured during indentation of the 68-degree trigonal diamond pyramid compressive test was carried out at a crosshead speed of 0.5mm/min. The results obtained were summarized as follows, 1. The highest hardness value was obtained in the CF group and the lowest value was obtained in the HM group. Hardness values showed no significant, difference between AG group and HM group but other groups showed the significant differences in each group(p<0.05). 2. Hardness number by Knoop pyramid were higher than those of 68-degree trigonal diamond pyramid. 3. Plastic deformation during the indentation of 68-degree trigonal diamond pyramid was the lowest in the CF group and the largest in the HM group. Results of Tukey test showed the significant difference between CF group and others; also between ZH and AF groups and AG and HM groups(p<0.05) 4. The highest compressive strength was obtained in the CF group and the lowest compressive strength was obtained in the ZH group. Compressive strength values showed no significant difference between CF group and ZH group but other groups showed the significant differences in each group(p<0.05).

• Restorative Dentistry and Endodontics
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• v.24 no.3
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• pp.473-481
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• 1999

This study evaluated the shear bond properties of IPS Empress glass ceramic to enamel and dentin surfaces with three ceramic surface treatments, and three resin cements. The influence of thermocycling was also investigated. The purpose of this study was to investigate the influences of resin cements, ceramic surface treatments, and thermocycling on shear bond properties. Ninety freshly extracted, noncarious human molars were selected for this study. The surface treatments of ceramic were etching <5.0% hydrofluoric acid, application of silane coupling agents(Tokuso Ceramic Primer, Clearfil porcelain bond, Monobond-S), and the combination of the two methods. Empress cylinders were bonded to enamel and dentin surfaces with three kinds of resin cements(Bistite resin cement, Panavia 21, Variolink). The specimens were aged in $37^{\circ}C$ distilled water for 24 hours. Half of the specimens were then thermocycled 500times between $5^{\circ}C$ and $55^{\circ}C$ with a dwell time of 15 seconds. Each specimen was debonded in shear mode and measured shear bond properties by using the universal testing machine(Zwick 020, Germany). The data were analyzed by SPSS/PC+(one-way ANOVA, Scheffe' s test and t-test). The results were as follows : 1. Without thermocyling, there was significant difference of shear bond strength to enamel surface between Bistite Resin Cement and Panavia 21 in case of etched and silane-treatment(p<0.05). 2. Without thermocyling, the shear bond strength of a group treated with silane and etching was significantly higher than that of a group treated with silane or etching with the application of Panavia 21 and Variolink(p<0.05). 3. A group treated with etching with the application of Variolink only showed a decrease of shear bond strength after thermocycling(p<0.05).