• Korean Journal of Veterinary Research
• /
• v.36 no.2
• /
• pp.405-415
• /
• 1996

This study was carried out to investigate the distribution of inclusion bodies in the tissues as well as to observe the general histopathological lesions of dogs infected with canine distemper. And also, the reliability of diagnostic values of inclusion bodies and the distribution of viral antigen in tissues were inspected by immunohistochemistry with monoclonal antibody. The results obtained were as follows; 1. Pneumonia observed in dogs infected with canine distemper virus was classified into interstitial, broncho-, and broncho-interstitial pneumonia histopathologically. Each occurring ratio was 35, 45 and 20%. 2. Histopathological classification of the canine distemper encephalitis was 20% in acute, 60% in subacute, and 20% in chronic encephalitis, respectively. 3. The organs in which inclusion bodies were predominantly distributed were stomach(82.6%), cerebellum(62.9%), lung(62.1%), cerebrum(50.0%), urinary bladder (46.1%), kidney(36.0%) and pancreas(25.0%). Intracytoplasmic inclusion bodies were mainly observed in the organs except the brain. 4. Canine distemper virus antigens were detected in the numerous tissues as well as in the inclusion bodies observed in the various organs. Antigen detection ratios in the lung, cerebellum and cerebrum were 68.9, 70.4 and 52.2%, respectively. These ratios were somewhat higher than those of inclusion bodies observed in the organs. 5. Canine distemper virus was mainly distributed in astrocytes and ependymal cells in the brain. These results suggested that the histopathologic diagnosis of canine distemper was reliable, and the spread of canine distemper virus in the brain was related with cerebrospinal fluid pathway.

• Korean Journal of Veterinary Research
• /
• v.37 no.2
• /
• pp.375-381
• /
• 1997

The purpose of the production of monoclonal antibodies aganist the Canine distemper virus(CDV) were perfect diagnosis and a new approach to treat canine distemper because the diagnosis and treatment of canine distemper were difficult. Canine distemper virus(CDV) was purified using saturated ammonium sulfate, and injected into hind footpads of BALB/c mouse. 12-15 days later, popliteal lymph node(PN) cells were harvested and fused with SP2/O myeloma cells. Characteristics of monoclonal antibodies were analysed. 1. 9 hybridomas produce the specific antibody against CDV. 2. 6 monoclonal antibodies are against intranuclear and cytoplasmic component of CDV, and 3 monoclonal antibodies are against cytoplasmic inclusions. 3. All monoclonal antibodies did not react with other 5 different viruses (CAV-I, CAV-II, CCV, CPV and CPIV) and react with another CDV-FXNO strain. 4. 3 monoclonal antibodies have neutralizing activity against CDV. 5. Antigenic difference was observed between CDV by IFA.

• Korean Journal of Veterinary Service
• /
• v.33 no.3
• /
• pp.213-221
• /
• 2010

The results from a total of 412 blood samples consisted of 187 samples from regular visiting group (RV), 94 samples from first visiting group (FV), 52 samples from abandoned group (A), 54 samples from special breeder group (SB), and 25 samples from preliminary breeder group (PB) showed that RV(94.7%) and SB(88.9%) groups had the higher levels of protective antibody, but PB (36.0%) group revealed the lowest level. Among 96 blood samples with lower protective antibody levels, 14 samples (14.6%), 72 samples (75.0%) and 10 samples (10.4%) were below the protective antibody levels to distemper/parvo-virus, distemper only and parvovirus only, respectively. These results implied that antibody to parvovirus was well generated than that to distemper. Eighty six samples (20.9%) showed the protective antibody titer under 1:96 to distemper and 24 samples (5.8%), the protective antibody titer under 1:40 to parvovirus.

• Korean Journal of Veterinary Pathology
• /
• v.1 no.2
• /
• pp.145-148
• /
• 1997

Two dead and two terminally sick badgers with signs of lacrimation and nasal discharge were submitted to the Pathology Division of the National Veterinary Research Institute for necropsy. The major gross findings included chronic dermatitis and pneumonia. histologically intracytoplasmic and intranuclear acidophilic inclusion bodies consistent with Canine Distemper (CD) virus particles in lung kidney urinary bladder skin foot pad stomach and small intestine. Additionally there were diffuse bronchointerstitial pneumonia hyperkeratosis of foot pads and focal non-suppurative encephalitis. Canine distemper infection in these badgers was further confirmed by immuofluorescent technique which demonstrated CD virus-specific antigens in lung and kidney sections.

• Korean Journal of Veterinary Research
• /
• v.39 no.4
• /
• pp.825-831
• /
• 1999

This study was performed to determine the etiological agents in concurrent disorders in gastrointestinal and respiratory tract. Most of dogs had clinical signs including nasal and ocular discharge, coughing, vomiting, and diarrhea. Of the 22 dogs, seropositive rates of each virus were 54.5% (12/22) against canine distemper virus, 90.9% (20/22) against canine adenovirus 1, 36.4% (8/22) against canine adenovirus 2, 18.2% (4/22) against canine parvovirus, 81.8% (18/22) against canine hepatitis virus and 59.1% (13/22) against canine coronavirus. Canine distemper virus and canine parvovirus infection were 54.6% (12/22) in histopathological examination. In addition, mixed infections of canine distemper virus and adenovirus 2 were 9.1% (2/22). While simple infection of canine adenovirus 2 were 9.1% (2/22). E coli and Staphylococcus spp were isolated in facts as a rate of 72.7% (16/22) and 40.9% (9/22), respectively. Conclusionally, it is also estimated that environmental stress might be one of the causative factors.

• Proceedings of the Korean Society of Veterinary Pathology Conference
• /
• 2003.10a
• /
• pp.44-44
• /
• 2003

Canine distemper virus (CDV) is a member of the genus Morbillivirus and the family Paramyxoviridae [3]. CDV is known to induce immunosuppression in affected animals by disrupting both humoral and cellular immunity [3]. This often results in secondary opportunistic infections. Activated toxoplasmosis develops in dogs whose immune systems have been damaged by CDV [3]. (omitted)

• Korean Journal of Veterinary Pathology
• /
• v.3 no.1
• /
• pp.61-64
• /
• 1999

A dead marten(Martes melampus) showing cough, ataxia and convulsion of hind limb followed by seizures, was submitted for diagnosis to the Pathology Division of the National Veterinary Research and Quarantine Service. In the gross lesions, lung was congested and consolidated and meningeal blood vessels were mildly congested. Histopathologic findings were diffuse interstitial pneumonia and nonsuppurative meningoencephalitis with malacia of cerebral and cerebellar white matter. Eosinophilic inclusion bodies were observed in neurons and astrocytes and oligodendroglial cells of brain and transitional epithelium of kidney. Using FA test and PCR method, specific antigens of canine distemper virus were demonstrated in the brain.

• Journal of Veterinary Clinics
• /
• v.21 no.1
• /
• pp.80-82
• /
• 2004

A 3 month-old male dog with clinical signs of anorexia, soft stool, ocular and nasal discharge, cough and respiratory distress was submitted to the Cheju National University for diagnosis. At necropsy, tan to pulp]e-red sublobar to lobar consolidations were presented in apical and cardiac lobe of lung. Histopathologically, severe diffuse bronchointerstitial pneumonia with necrotic bronchiolitis was noted in the lung. The demyelinating encephalitis and astrocytosis were presented in cerebellum and cerebrum. Numerous round, ovoid or cluster of tachyzoites were also identified in alveolar lumen, alveolar wall and cytoplasm of macrophages in the lung. The orgasnisms were demonstrated as Toxoplasma (T) gondii by immunohistochemistry. Intranuclear or intracytoplasmic eosinophilic inclusion bodies were seen in the glial cells of the cerebellum. Canine distemper virus (CDV) specific antigens were demonstrated in the cerebellum by the immunohistochemistry. In our knowledge, this is believed to be the first confirmed report of co-infection of CDV and T gondii in dog in Korea.

• Korean Journal of Veterinary Service
• /
• v.41 no.1
• /
• pp.51-55
• /
• 2018

Herein, we report a case of badgers showing high morbidity and mortality rate due to the mixed infection of canine distemper virus (CDV) and Streptococcus canis (S. canis) in a farm where wild animal, badger, is being reared for herbal medicine. During the period of about one month, 120 out of 320 badgers showed severe respiratory symptoms and died, and 3 bodies were submitted to the Animal and Plant Quarantine Agency for disease diagnosis. The lung with the most severe necropsy findings failed to collapse and showed dark reddening and had yellowish nodules on the cut surface. The characteristic and common histopathologic findings include multifocal necrosis with hemorrhage of the lung, severe lymphoid depletion of the spleen and intracytoplasmic or intranuclear inclusion bodies in almost all organs. Finally, CDV and S. canis were identified by immunohistochemistry and bacterial isolation, respectively. This is the first mixed infection case of CDV and S. canis in badgers being raised on the farm.

• Korean Journal of Veterinary Service
• /
• v.37 no.4
• /
• pp.273-280
• /
• 2014

Investigations of hematologic and canine distemper virus, parvovirus antibody titer for DongGyeongi were performed. This study was conducted to determine into feeding and management blood values on DongGyeongi. Blood samples were collected from 110 healthy dogs (male 60, female 50). The diagnostic virus disease and hematologic results were classified by age, sex, color. Although gender differences were not apparent, complete blood cell counts analyses were performed and analyzed accordingly. Statistically significant differences (P<0.05) specific to age (<2). Also, The canine distemper virus and parvovirus antibody titer were correlation to age dependent (P<0.05). In conclusion, data obtained from this study may be valuable as a standard for interpretation of the results in hematologic and major viral antibody titer analysis of DongGyeongi populations. For the management of DongGyeongi, the programs will be used for the epidemic disease prevention.