• Journal of Embryo Transfer
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• v.30 no.2
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• pp.109-114
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• 2015

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.358A>T) of cluster-of-differentiation antigen 9 (CD9) gene reported to be significant association with MOT. Also, CD9 gene was expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as candidate gene for boar semen. This study was conducted to evaluate the pig SNP (g.358A>T) of CD9 gene as a positional controlling for semen parameters of post-thawed boar semen. To results, the g.358A>T SNP of the CD9 gene was significantly associated with the traits such as MOT, curve linear velocity, straight line velocity, average path velocity and amplitude of lateral head displacement. Particularly, the g.358A>T SNP significantly has the highest association with MOT and animals with AA genotype (p<0.001). Therefore, we suggest that the g.358A>T in the intron 6 region of the porcine CD9 may be used as a molecular marker for Duroc boar Post-thawed semen quality, although its functional effect was not defined yet.

• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.189-192
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• 2012

Male factor infertility or sub-fertility contributed half of all cases of infertility while the semen abnormality is the current topic of argument. Conventional analysis of semen showed poor correlation with fertility. Therefore, evaluation of current semen analysis method is necessary to improve standards of semen assessment. The goal of this study was to investigate that correlation between motion kinematic before and after capacitation and litter size in porcine. Sperm motility and kinematics were measure by computer-assisted sperm analysis (CASA). The motility of spermatozoa was positively correlated with curvilinear velocity (VCL), average path velocity (VAP), and mean amplitude of head lateral displacement (ALH) (p<0.05). Where as VCL positively correlated with VSL, VAP and ALH (p<0.01). Straight-line velocity (VSL) was positively correlated with VAP and ALH (p<0.01). VAP was significantly positively correlated with ALH (p<0.01). Also, we found significant positive correlation among variation of VSL, VAP and ALH (p<0.05). No motility and kinematic parameter are correlated with litter size. However, litter size was significantly correlated with breed (p<0.05). Our results suggested that analysis of sperm motility and kinematics using CASA is questionable for prediction of litter size. However, it has some practical importance to evaluate semen commercially.

• Clinical and Experimental Reproductive Medicine
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• v.21 no.1
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• pp.1-11
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• 1994

The occurrence and time course of capacitation, acrosomal loss, and hyperactivated motility require quantitative definition in order to characterize fertile human sperm. Recently the method has been developed to estimate the quality of spermatozoa by using kinematic parameters such as curvilinear velocity(VCL), average path velocity(VAP), linearity(LIN), straightness(STR), amplitude of lateral head displacement(ALH), and beat cross frequence(BCF) from Computer Assisted Sperm Analysis (CASA). In this study, using the Hamilton Thorn motility analyzer HTM 2030(Hamilton Thorn Research, Beverly, MA), we attempted to identify the spermatozoa with hyperactivated motility (HA) objectively and to monitor hyperactivation of human spermatozoa during incubation in capacitating media and after treatment of calcium ionophore as compared with acrosome status. And we examined whether HA are related to the result of SPA. Semen samples obtained from 16 healthy men were prepared by swim up technique and preincubated in a capacitating media(modified BWW medium) for 5 hours and treated with calcium ionophore solution. The acrosome reaction was detected with PSA-FITC labelling of the acrosome and in vitro sperm ferilizing capacity was assessed by the zona free hamster ovum penetration assay (SPA). The incidence of hyperactivated sperm was 2.6% in fresh semen, 14.3% of the swim up population, 13.7% after 5h of incubation. Significant increase of percentage of hyperactivated sperm was observed after the incubation (p<0.05) but after treatment, no significant changes of percentage of hyperactivated sperm(l1.8%) in contrast to significant rise in the percentage of acrosome reacted cells. Correlation analysis failed to show any significant relationship between the percentage of sperm with HA and SPA score. In conclusion, although no direct correlations were found between the results of SPA and HA, hyperactivation of sperm is associated with capacitation and monitoring hyperactivated sperm will be expected as a method of evaluating the functional quality of sperm such as SPA.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.2
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• pp.199-208
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• 2010

A friction damper installed at a building shows nonlinear behavior since its stick and slip states are occurred repeatedly depending on the amplitude of external loadings to dissipate input energy. Friction damping is existed for the building with a friction damper. In additionally viscous one is inherently included. Therefore, the building installed in such combined damping is quite involved to find the analytical solution. In this study, first, displacement and acceleration characteristics are identified based on the exact solution for a single-degree-freedom building with a friction damper having both friction and viscous damping. Second, in free vibration, the equivalent viscous damping ratio is obtained by the energy dissipation. Third, numerical analysis is carried out to find response configuration with various friction force ratios. Fourth, corresponding equivalent viscous damping ratio is derived with the finding that the response reaches into steady-state for both friction and viscous damped structure. It is deduced using balance of input external energy and output dissipation energy for steady-state response. Finally, the equivalent viscous damping ratios of free or harmonic vibration are verified through nonlinear analysis.

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.297-302
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• 2013

Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of genetically elite populations. Castration, catastrophic injury, sudden death or any other event that makes semen collection or mating impossible may prematurely terminate a stallion reproduction. Stallion epididymal spermatozoa vary widely in the loss of progressive motility, acrosomal integrity, and viability during freezing and thawing. The objective of this work was to investigate the effect of (1) freezing package types on cryopreservation efficiency, (2) thawing temperatures (37, 56 or $70^{\circ}C$) on Computer Assisted Sperm Analysis (CASA) parameters and (3) post-thawing incubation time (0, 1, 2 or 4h) on castrated stallion epididymis. Post-thawed sperm motility ranged between 59.69% and 64.28% ($56^{\circ}C$ and $37^{\circ}C$) in various thawing temperatures. When stallion epididymis sperm was frozen, straw was better than freezing tube on VCL (Velocity of Curvilinear Line) and VAP (Velocity of Average Path) parameter. Higher percentage of motility was observed at $37^{\circ}C$ thawing temperature even though no significant difference was observed among various temperatures. The motility, VCL, ALH (Amplitude of Lateral Head displacement), VAP, BCF (Beat-Cross Frequency) and STR (Straightness index) parameter of post-thawed sperm were significantly decreased by increasing the incubation time for all thawing temperatures. The present study showed that type of freezing package (Straw vs. Freezing tube) was not significantly different on cryopreservation efficiency. Furthermore, stallion epididymal spermatozoa frozen-thawed at $37^{\circ}C$ for 1 min resulted the highest proportion of motility and velocity movement. In addition, motility and viability of frozen-thawed stallion epididymal spermatozoa were also decreased by incubation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.94-101
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• 2019

In this study, the FEM technique was applied to design the shape of the horn that transmits ultrasonic vibration energy to the base material, and the shape of the welding horn with a one-wavelength bar shape used in the 20kHz region was designed. The shape design of the horn was performed by applying the rod longitudinal vibration theory to Ansys APDL (Ansys Parametric Design Language). Twenty-five models were designed using the ratio of the area of the input and output surfaces of the vibration and the length of the horn to derive the appropriate horn shape. The horn was designed with a total length of 130mm, a step length of 65mm, and an output area of 28.79mm. The horn was fabricated using the optimized dimensions, and the vibration and displacement characteristics of the horn were evaluated using the measurement system. Finally, a uniform longitudinal step horn was designed, and more than 97.4% of the uniformity of the tip was secured. The amplitude ratio of the optimized horn was improved by 51%.

• Journal of Environmental Science International
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• v.23 no.10
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• pp.1719-1729
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• 2014

This study is carried out in order to bridge the gap to understand the relationships between South Asian and East Asian monsoon systems by comparing the summer (June-September) precipitation of Nepal and South Korea. Summer monsoon precipitation data from Nepal and South Korea during 30 years (1981-2010) are used in this research to investigate the association. NCEP/NCAR reanalysis data are also used to see the nature of large scale phenomena. Statistical applications are used to analyze these data. The analyzed results show that summer monsoon precipitation is higher over Nepal ($1513.98{\pm}159.29mm\;y^{-1}$) than that of South Korea ($907.80{\pm}204.71mm\;y^{-1}$) and the wettest period in both the countries is July. However, the coefficient of variation shows that amplitude of interannual variation of summer monsoon over South Korea (22.55%) is larger in comparison to that of Nepal (10.52%). Summer monsoon precipitation of Nepal is found to be significantly correlated to that of South Korea with a correlation coefficient of 0.52 (99% confidence level). Large-scale circulations are studied to further investigate the relationship between the two countries. wind and specific humidity at 850 hPa show a strong westerly from Arabian Sea to BOB and from BOB, wind moves towards Nepal in a northwestward direction during the positive rainfall years. In case of East Asia, strong northward displacement of wind can be observed from Pacific to South Korea and strong anticyclone over the northwestern Pacific Ocean. However, during the negative rainfall years, in the South Asian region we can find weak westerly from the Arabian Sea to BOB, wind is blowing in a southerly direction from Nepal and Bangladesh to BOB.

• Nuclear Engineering and Technology
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• v.20 no.2
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• pp.88-104
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• 1988

The FUel Rod Analysis(FURA) code is developed using two-dimensional finite element methods for axisymmetric and plane stress analysis of fuel rod. It predicts the thermal and mechanical behavior of fuel rod during normal and load follow operations. To evaluate the exact temperature distribution and the inner gas pressure, the radial deformation of pellet and clad, the fission gas release are considered over the full-length of fuel rod. The thermal element equation is derived using Galerkin's techniques. The displacement element equation is derived using the principle of virtual works. The mechanical analysis can accommodate various components of strain: elastic, plastic, creep and thermal strain as well as strain due to swelling, relocation and densification. The 4-node quadratic isoparametric elements are adopted, and the geometric model is confined to a half-pellet-height region with the assumption that pellet-pellet interaction is symmetrical. The pellet cracking and crack healing, pellet-cladding interaction are modelled. The Newton-Raphson iteration with an implicit algorithm is applied to perform the analysis of non-linear material behavior accurately and stably. The pellet and cladding model has been compared with both analytical solutions and experimental results. The observed and predicted results are in good agreement. The general behavior of fuel rod is calculated by axisymmetric system and the cladding behavior against radial crack is used by plane stress system. The sensitivity of strain aging of PWR fuel cladding tube due to load following is evaluated in terms of linear power, load cycle frequency and amplitude.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.5
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• pp.128-135
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• 2021

Thin-walled tubes have been widely used as energy absorbing devices because they are light and have high energy-absorption efficiency. However, the downside is that conventional thin-walled tubes usually exhibit an excessive initial peak crushing force (IPCF) and a large fluctuation in the load-displacement curve, and thus lack stability as energy absorbing devices. Corrugated tubes were introduced to reduce IPCF and to increase the stability of collision energy-absorbing devices. Since the performance of corrugated tubes is highly influence by geometry, design optimization methods can be utilized to optimize the performance of corrugated tubes. In this paper, we utilize shape design optimization based on an adaptive surrogate model for crashworthiness analysis. The amplitude and wavelength of the corrugation, as well as curvature changes in the features, are the design variables. A morphing methodology is adopted to perform shape design parameterization. Through numerical examples, we compare optimal design results based on the adaptive surrogate model, with optimal results based on conventional surrogate models, and we show that direct optimal design methods produce more efficient results.

• 한국체육학회지인문사회과학편
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• v.58 no.6
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• pp.449-459
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• 2019

The purpose of this study is to identify how postural mechanics affects postural control on balance and stability by using frequency analysis technique from the kinematic data acquired during the one leg standing posture. For this purpose, the experimental group consisted of two groups, the normal group (n=6) and the national Gymnastics group (n=6). Displacement data of CoP were analyzed by frequency analysis of rambling (RM) and trembling (TR) by FFT signal processing. As a results, there was a significant difference in evaluating the stabilization index between the two groups with the eyes open and closed one leg stnading (p <.05). The cause of the difference was found to be the output of the maximum amplitude of RM (f1) and TR (f2) (p <.05). In particular, in the low frequency RM of 8-9 Hz, which is a natural frequency of signal wave involved in postural feedback feedback, the main frequency appeared to be performs the exercise mechanism of stable brain posture control. And in the high frequency TM of 120-135 Hz, it is considered that the adaptation of the reflective muscle response is minimized to minimize posture shaking. In conclusion, this study provides evidence for the intrinsic main frequencies according to the postural control ability which affects the CNS in one leg standing.