• Title/Summary/Keyword: dispersed concentration

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A Study on the Solubilization of $\alpha$-Chymotrypsin Using AOT Reverse Micelles; Effects of pH and salts (AOT 역미셀을 이용한 $\alpha$-chymotrypsin의 가용화에 대한 연구;pH와 염의 영향)

  • 노선균;강춘형
    • KSBB Journal
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    • v.15 no.6
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    • pp.664-669
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    • 2000
  • Micellar aggregates are known to be useful for the selective isolation of biologically active materials such as amino acids, proteins, and enzymes from crude mixtures sparsely dispersed in water. In this study, the effects of pH, salt type and its concentration on the solubilization of $\alpha$-chymotrypsin into the organic micellar phase, which consisted of AOT (sodium 야(2-ethylhexy)sulfosuccinate) and iso-octane, were comprehensively examined. It was found that maximum extraction efficiency was attained at a pH below the isoelectric point of $\alpha$-chymotrypsin; at pH=5.0 for NaCl and KCl, and at pH=7.0 for $CaCl_2$and $MgCl_2$. In order to avoid complications stemming from the precipitationof protein at low pH interfaces, the protein concentrations in the organic and aqueous phases were directly measured. The size of the micelle water pool was estimated by measuring the molar ratio of the surfactant to the water, W(sub)o. The resulting values of W(sub)o were nearly constant at 30 and 19 for NaCl and KCl, respectively, and were independent of pH. The addition of 1:2 salts like $MgCl_2$and $CaCl_2$ led to much lower, but a constant value of, W(sub)o than the 1:1 salts.

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Preconcentration and Determination of Trace Copper(II) and Lead(II) in Aqueous Solutions by Adsorption on Ca-Alginate Bead (알긴산칼슘 비드 상 흡착에 의한 흔적량 구리(II)와 납(II)의 동시 농축 및 정량)

  • Choi, Jong-Moon;Choi, Sun-Do
    • Journal of the Korean Chemical Society
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    • v.48 no.6
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    • pp.590-598
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    • 2004
  • The preconcentration and determination of trace Cu(II) and Pb(II) on calcium alginate beads in aqueous solution were studied. A calcium alginate beads were prepared by adding an alginic acid to sample solution contained Ca(II). Some following conditions were optimized: the pH of sample solution, amount of alginic acid, and stirring time for effective adsorption; the type and concentration of acid, and sonication time in an ultrasonic vibrator for the perfect de-sorption. A sample solution was prepared with Cu(II) and Pb(II) in DI water. And Ca(II) and ethanol was added into the sample solution. The pH of the final sample solution was controlled with buffer solution. The alginic acid were dispersed in the sample solution by a magnetic stirrer. This mixture was stored in room temperature for 30 min to form a calcium alginate. After the beads were filtered and washed on a membrane filter, the analytes were redissolved from the beads by an ultrasonic vibration of 10 minutes in 1.0M $HNO_3$ solution. The effect of diverse ions on the adsorption of analytes were studied. This procedure was applied for the analysis of two real samples. The recoveries in spiked samples were $90.4{\sim}104.3%$ for analytes.

SCR Reaction Activity and SO2 Durability Enhancement in Accordance with Manufacturing Conditions of the V/TiO2 Catalysts (V/TiO2 촉매의 제조조건에 따른 SCR 반응활성 및 SO2 내구성 증진에 대한 연구)

  • Lee, Seung Hyun;Seo, Jeong Uk;Byeon, Sang Geun;Hong, Sung Chang
    • Clean Technology
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    • v.22 no.2
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    • pp.114-121
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    • 2016
  • In this studies, SCR reaction activity and SO2 durability enhancement study on manufacturing conditions of the V/TiO2 catalyst was carried out for the removal of nitrogen oxides generated in the combustion furnace. The catalysts are characterized by XPS, Raman, H2-TPR and SO2-TPD. When the vanadium was contained of 2 wt%, it showed excellent SO2 durability and catalytic activity. and When the tungsten is added as a promotor, the enhancement of reducing ability at a low temperature and reduction of SO2 adsorption capacity improved the reaction activity and SO2 durability. V/W/TiO2 are prepared by the lower pH of vanadium solution, vanadium was highly dispersed on the surface and inhibited the formation of crystalline V2O5. in addition, it was confirmed that this catalyst can be used as excellent resistance to high concentration of CO in the combustion furnace.

Application of Hot Spot Analysis for Interpreting Soil Heavy-Metal Concentration Data in Abandoned Mines (폐금속 광산의 토양 중금속 오염 조사 자료 해석을 위한 핫스팟 분석의 적용)

  • LEE, Chae-Young;KIM, Sung-Min;CHOI, Yo-Soon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.22 no.2
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    • pp.24-35
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    • 2019
  • In this study, a hotspot analysis was conducted to suggest a new method for interpreting soil heavy-metal contamination data of abandoned metal mines according to statistical significance level. The spatial autocorrelation of the data was analyzed using the Getis-Ord $Gi{\ast}$ statistic in order to check whether soil heavy metal contamination data showing abnormal values appeared concentrated or dispersed in a specific space. As a result, the statistically significant data showing abnormal values in the mine area could be classified as follows: (1) the contamination degree and the hotspot value (z-score) were both high, (2) the contamination degree was high but the z-score was low, (3) the contamination degree was low but the z-score was high and (4) the contamination degree and the z-score were both low. The proposed method can be used to interpret the soil heavy metal contamination data according to the statistical significance level and to support a rational decision for soil contamination management in abandoned mines.

Effect of Aromatic Additives on the Coke Reduction and the Asphaltene Conversion in a Slurry-phase Hydrocracking (슬러리상 수첨분해 반응에서 아로마틱 유분 첨가에 따른 코크 저감 및 아스팔텐 전환 특성)

  • Lim, Suk Hyun;Go, Kang Seok;Nho, Nam Sun;Lee, Jae Goo
    • Korean Chemical Engineering Research
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    • v.57 no.2
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    • pp.244-252
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    • 2019
  • This study investigated the effect of addition of aromatics such as Toluene/LCO/resin on the coke depression and asphaltene conversion. The experiment was carried out with vacuum residue as a feedstock with Molybdenum dispersed catalysts under the slurry-phase hydrocracking condition (Temp. of $425^{\circ}C$, $H_2$ pressure of 80 bar at $80^{\circ}C$, reaction time of 4 hr, Mo-concentration of 500 ppm). As results, the coke reduction was shown to be similar irrespective of types of aromatics, while asphaltene was more converted to gas and maltene when LCO and resin with higher dipole moment were added. The addition of aromatics with change of reaction time showed no difference in terms of depression of coke formation. But the addition of LCO rather increased the coke yield after 2 hr. And it was found that asphaltene in liquid phase had the higher aromaticity index so that asphaltene is difficult to disperse in oil phase.

The Experimental Assessment of Influence Factors on KLS-1 Microwave Sintering (한국형 인공월면토(KLS-1) 마이크로파 소결에 미치는 영향인자에 관한 실험적 연구)

  • Jin, Hyunwoo;Lee, Jangguen;Ryu, Byung Hyun;Shin, Hyu-Soung;Kim, Young-Jae
    • Journal of the Korean Geotechnical Society
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    • v.37 no.2
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    • pp.5-17
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    • 2021
  • The Moon has been an attractive planet as an outpost for deep space exploration since He-3 and water ice which can be used as energy resources were discovered. In-Situ Resource Utilization (ISRU) construction material fabrication method is required for sustainable space planet exploration. In this paper, the possibility of microwave sintering technology for construction material fabrication was evaluated using lunar regolith that can be easily collected from the Moon surface. Experimental assessment of the influence factors on microwave sintering was conducted using a hybrid sintering system for efficient processing. The heat distribution in the furnace was observed using thermal paper that is coated with a material formulated to change color when exposed to heat. Based on this result, sintered cylindrical KLS-1s with a diameter of 1 cm and a height of 2 cm were fabricated. Densities were measured for the sintered KLS-1s under rotating turntable conditions that have an effect of microwave dispersion. The more dielectrics were arranged, the more microwaves were dispersed reducing the heat concentration, and thus a uniformity of sintered KLS-1s was enhanced.

Implementation of A Vibration Notification System to Support Driving for Drivers with Cognitive Delay Impairment

  • Gyu-Seok Lee;Tae-Sung Kim;Myeong-Chul Park
    • Journal of the Korea Society of Computer and Information
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    • v.29 no.4
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    • pp.115-123
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    • 2024
  • In this paper, we propose a vibration notification system that combines navigation information and wearable bands to ensure safe driving for the transportation vulnerable. This system transmits navigation driving information to a linked application, converts it into a vibration signal, and provides notifications through a wearable band. Existing navigation systems focus on providing route guidance and location information, so the driver's concentration is dispersed, and safety and convenience are deteriorated, especially for those with mobility impairments, due to standard vision and delayed recognition of stimuli, resulting in an increasingly high traffic accident rate. To solve this problem, navigation driving information is converted into vibration signals through a linked application, and vibration notifications for events, left turns, right turns, and speeding are provided through a wearable band to ensure driver safety and convenience. In the future, we will use cameras and vehicle sensors to increase awareness of safety inside and outside the vehicle by adding a function that provides notifications with vibration and LED when the vehicle approaches or recognizes an object, and we will continue to conduct research to build a safer driving environment. plan.

MECHANISM IN ANTIBACTERIAL ACTIVITY OF POLYPHOSPHATES AGAINST PORPHYROMONAS ENDODONTALIS (Porphyromonas endodontalis에 대한 Polyphosphate의 항균기전에 관한 연구)

  • Choi, Sung-Baik;Park, Sang-Jin;Choi, Gi-Woon;Choi, Ho-Young
    • Restorative Dentistry and Endodontics
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    • v.25 no.4
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    • pp.561-574
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    • 2000
  • Poly-P has been used to prevent decomposition of foods and has been shown to have inhibitory effect on the growth of gram positive bacteria. The purpose of this study was to evaluate the effect of poly-P on the growth of Porphyromonas endodontalis, a gram negative obligate anaerobic rod, endodontopathic bacterium. P. endodontalis ATCC 35406 was in BHI broth containing hemin and vitamin K with or without poly-P. Inhibitory effect of each poly-P which was added at the beginning(lag phase) or during(exponential phase) the culture, MIC(minimum inhibitory concentration) was determined by measuring the optical density of the bacterial cell at 540nm. Viable cell counts were measured to determined whether poly-P has a bactericidal effect. Leakage of intracellular nucleotides from P. endodontalis was determined at 260nm and morphological change of P. endodontalis was observed under the TEM(transmission electron microscope). Binding of 32P-labeled poly-P to P. endodontalis was examined. SDS-polyacrylamide gel electrophoresis and zymography were performed to observe the changes in protein and enzyme profiles of P. endodontalis, respectively. The results from this study were as follows : 1. The minimal inhibitory concentration(MIC) of poly-P to P. endodontalis appeared to be 0.04~0.05%. 2. Poly-P added to the P. endodontalis culture during the exponential phase of P. endodontalis was as much effective as poly-P added at the begining of the culture, suggesting that the antibacterial effect of poly-P is not much dependent on the initial inoculum size of P. endodontalis. 3. Poly-P are bactericidal to P. endodontalis, demonstrating the decrease of the viable cell counts. 4. Intracellular nucleotide release from the P. endodontalis, was not increased in the presence of poly-P and was not reversed by the addition of divalent cations like $Ca^{2+}$ and $Mg^{2-}$. 5. Under the TEM, it was observed that fine electro-dense materials were prominent in the poly-P grown P. endodontalis, appearing locally in the cell, and the materials were more abundant and more dispersed in the cell as the incubation time with poly-P increased. In addition, highly electron dense granules accumulated in many poly-P grown cells, most of which were atypical in their shape. 6. Binding of 32P-labeled poly-P to P. endodontalis appeared to be 32.8 and 45.5 and 53.4% at 30 minutes, 1 hours and 2 hours, respectively. 7. In the presence of poly-P. the synthesis of proteins with apparent molecular masses of 25, 27, 35, 45 was lost or drastically decreased whereas expression of a protein with an apparent molecular mass of 75 was elevated. 8. Proteolytic activity of P. endodontalis was decreased by poly-P. The overall results suggest that use of poly-P may affect the growth of P. endodontalis, and the anti-bacterial activity of poly-P seems largely bactericidal. Changes in shape, protein expression, and proteolytic activity of P. endodontalis by poly-P may be directly and indirectly attributed to the antibacterial effect of poly-P. Further studies will be needed to confirm the effect of poly-P.

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Evaluation of the Usefulness of Virtual Reality Equipment for Relieving Patients' Anxiety during Whole-Body Bone Scan (전신 뼈 검사 환자의 불안감 해소를 위한 가상현실 장비의 유용성 평가)

  • Kim, Hae-Rin;Kim, Jung-Yul;Lee, Seung-Jae;Baek, Song-Ee;Kim, Jin-Gu;Kim, Ga-Yoon;Nam-Koong, Hyuk;Kang, Chun-Goo;Kim, Jae-Sam
    • The Korean Journal of Nuclear Medicine Technology
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    • v.26 no.1
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    • pp.27-32
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    • 2022
  • Purpose When performing a whole-body bone scan, many patients are experiencing psychological difficulties due to the close distance to the detector. Recently, in the medical field, there is a report that using virtual reality (VR) equipment can give pain relief to pediatric patients with weak concentration or patients receiving severe treatment through a distraction method. Therefore, in this paper, VR equipment was used to provide psychological stability to patients during nuclear medicine tests, and it is intended to evaluate whether it can be used in clinical practice. Materials and Methods As VR equipment, ALLIP Z6 VR (ALLIP, Korea) was used and the experiment was conducted after connecting to a mobile phone. The subjects were 30 patients who underwent whole-body bone examination from September 1, 2021 to September 30, 2021. After intravenous injection of 99mTc-HDP, 3 to 6 hours later, VR equipment was put on and whole body images were obtained. After the test, a survey was conducted, and a Likert scale of 5 points was used for psychological anxiety and satisfaction with VR equipment. Hypothesis verification and reliability of the survey were analyzed using SPSS Statistics 25 (IBM, Corp., Armonk, NY, USA). Results Anxiety about the existing whole-body bone test was 3.03±1.53, whereas that of anxiety after wearing VR equipment was 2.0±1.21, indicating that anxiety decreased to 34%. When regression analysis of the effect of the patient's concentration on VR equipment on anxiety about the test, the B value was 0.750 (P<0.01) and the t value was 6.181 (P<0.01). decreased and showed an influence of 75%. In addition, overall satisfaction with VR equipment was 3.76±1.28, and the intention to reuse was 66%. The Cronbach α value of the reliability coefficient of the questionnaire was 0.901. Conclusion When using VR equipment, patients' attention was dispersed, anxiety was reduced, and psychological stability was found. In the future, as VR equipment technology develops, it is thought that if the equipment can be miniaturized and the resolution of VR content images is increased, it can be used in various clinical settings if it provides more realistic stability to the patient.

The Effect of Au Addition on the Hardening Mechanism in Ag-25wt% Pd-15wt% Cu (Ag-25wt% Pd-15wt% Cu 3원합금(元合金) 및 Au 첨가합금(添加合金)의 시효경화특성(時效京華特性))

  • Bea, B.J.;Lee, H.S.;Lee, K.D.
    • Journal of Technologic Dentistry
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    • v.20 no.1
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    • pp.37-49
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    • 1998
  • The specimens used were Ag-25 Pd-15 Cu ternary alloy and Au addition alloy. These alloys were melted and casted by induction electric furnace and centrifugal casting machine in Ar atmosphere. These specimens were solution treated for 2hr at $800^{\circ}C$ and were then quenched into iced water, and aged at $350{\sim}550^{\circ}C$ Age- hardening characteristics of the small Au-containing Ag-Pd-Cu dental alloys were investigated by means of hardness testing. X-ray diffraction and electron microscope observations, electrical resistance, ergy dispersed spectra and electron probe microanalysis. Principal results are as follows : Hardening occured in two stages, i.e., stage I in low temperature and stage II in high temperature regions, during continuous aging. The case of hardening in stage I was due to the formation of the $L1_0$ type face-centered tetragonal PdCu-ordered phase in the grain interior and hardening in stage I was affected by the Cu concentration. In stage II, decomposition of the ${\alpha}$ solid solution to a PdCu ordered phase($L1_0$ type) and an Ag-rich ${\alpha}2$ phase occurred and a discontinuous precipitation occurred at the grain boundary. From the electron microscope study, it was conclued that the cause of age-hardening in this alloy is the precipitation of the PdCu ordered phase, which has AuCu I type face-centered tetragonal structure. Precipetation procedure was ${\alpha}{\to}{\alpha}+{\alpha}_2+PdCu {\to}{\alpha}_1+{\alpha}_2+PdCu$ at Pd/Cu = 1.7 Ag-Pd-Cu alloy is more effective dental alloy as ageing treatment and is suitable to isothermal ageing at $450^{\circ}C$.

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