• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.281-287
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• 1999

Codonopsis lanceolata ("Deoduck" in Korea) is a perennial herb, and belongs to family, Campanulaceae. Its taproot is used a good source of a wild vegetable as well as an herbaceous medicine. In this study, to develop a bialaphos-resistant transgenic Codonopsis, seed germination mechanism and somatic embryogenesis of the plant were investigated, and Agrobacterium-mediated transformation with bar gene encoding phosphinothricin acetyltransferase (PAT) was performed. Attempt were made to regenerate plant from cells via somatic embryogenesis. When the cotyledons, nodes and leaf disks were cultured on MS medium containing 2,4-D and zeatin, embryogenic calli were induced. Upon transferring the somatic embryos to N6 solid medium without plant growth regulators, they developed into plantlets under continuous illumination. All plants were dead on MS basal medium containing 10 mg/L phosphinothricin (PPT) and Basta, respectively. The explants did not produce calli in the medium containing 200 mg/L kanamycin. The explants were cocultured with Agrobacterium tumefaciens for 2 days, and transformants were selected in MS basal medium containing 1.0 mg/L 2,4-D, 100 mg/L kanamycin and 500 mg/L carbenicillin. After the selection, embryogenic calli were induced and then somatic embryos were produced by subsequent subculturing. The somatic embryos were germiated on N6 basal medium containing 200 mg/L kanamycin and 500 mg/L carbenicillin. PCR analysis showed that nptII and bar genes were introduced in the Deoduck transformants. After the confirmation of bar gene expression in RNA and protein level, the transgenic Deoduck will be used to study the genetics of filial generation with the herbicide control gene, bar.gene, bar.

• Journal of the Korean Wood Science and Technology
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• v.34 no.1
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• pp.23-31
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• 2006

X-ray transit materials with straight path and the its intensity is proportional to the density of materials. Therefore, X-ray has been extensively used as a nondestructive evaluation (NDE) method in various fields. This study was carried out for development of a portable X-ray CT (computed-tomography) system to detect deteriorations of wood members in buildings. Based on the results of our previous study, a procedure of CT image reconstruction was established In order to verify the applicability of developed system, CT images of three wood disks were reconstructed by newly developed procedure and compared with the prototypes. From the results of this study, it was shown that the newly developed system could be used not only to determine the shape, size, and position of defects, but also to find the density distribution in cross section of wood structure members. The density distribution may be utilized to clarify the reason of wood deterioration and to provide the preventive method on how to treat or repair wood buildings. Because it was initial stage of system development, there were some limitations concerned with measuring equipment and image reconstruction algorithm. Especially, measuring time including equipment setup time was longer and measuring accuracy was lower than we expected. Therefore, we planned some additional studies on improvement of equipment and algorithm to enhance the capability of X-ray CT system.

• Journal of KIISE:Computing Practices and Letters
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• v.10 no.2
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• pp.134-145
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• 2004

With rapid growth in the amount of data transferred on the Internet, traditional storage systems have reached the limits of their capacity and performance. SAN (Storage Area Network), which connects hosts to disk with the Fibre Channel switches, provides one of the powerful solutions to scale the data storage and servers. In this environment, the maintenance of data consistency among hosts is an important issue because multiple hosts share the files on disks attached to the SAN. To preserve data consistency, each host can execute the disk I/O whenever disk read and write operations are requested. However, frequent disk I/O requests cause the deterioration of the overall performance of a SAN cluster. In this paper, we introduce a SANtopia global buffer manager to improve the performance of a SAN cluster reducing the number of disk I/Os. We describe the design and algorithms of the SANtopia global buffer manager, which provides a buffer cache sharing mechanism among the hosts in the SAN cluster. Micro-benchmark results to measure the performance of block I/O operations show that the global buffer manager achieves speed-up by the factor of 1.8-12.8 compared with the existing method using disk I/O operations. Also, File system micro-benchmark results show that SANtopia file system with the global buffer manager improves performance by the factor of 1.06 in case of directories and 1.14 in case of files compared with the file system without a global buffer manager.

• Journal of KIISE:Computing Practices and Letters
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• v.9 no.6
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• pp.635-645
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• 2003

One of very important features that are necessarily supported by clustered parallel computer systems is a single I/O system image in which users can access both the local and remote I/O resources transparently. In this paper, we propose an efficient disk sharing technique supporting a single disk I/O system image architecture. The design separates the I/O subsystem of a cluster into the file system and a set of virtual hard disk drivers. The virtual hard disk driver deals with a hard disk in the remote node as a local hard disk. All services provided by it are performed in the device driver level without any modification of file systems. Users can, therefore, access all the disks in the cluster regardless of their locations. Our virtual hard disk driver is implemented under the linux, and also tested in a linux cluster system. We find by experiments that it can successfully support a single disk I/O space, and at the same time it shows better performance than NFS. We are sure that this paper can be a guideline for single I/O space of other devices to be easily constructed.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.11
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• pp.797-809
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• 2006

To support the QoS streams for large scale clients, the internal resources of VOD servers should be utilized based on the characteristics of the streaming media service. Among the various resources in the server, the main memory is used for the buffer space to the media data loaded from the disks and the buffer hit ratio has a great impact upon the server performance. However, if the buffer data with high hit ratio are replaced for the new media data as a result of the number of clients and the required movie titles are increased, the negative impact on the scalability of server performance is occurred. To address this problem, the buffer replacement policy considers the intrinsic characteristics of the streaming media such as the sequential access to large volume data and the highly disproportionate preference to specific movies. In this paper, the preference-based segment buffer replacement policy is proposed in the cluster-based VOD server to exploit the characteristics of the streaming media. Since the proposed method reflects both the temporal locality by the clients' preference and the spatial locality by the sequential access to media data, the buffer hit ratio would be improved as compared to the existing buffer replacement policy. The enhanced buffer hit ratio causes the fact that the performance scalability of the cluster-based VOD server is linearly improved as the number of cluster nodes is increased.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2018.06a
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• pp.75-75
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• 2018

Commercially pure titanium (CP-Ti) and Ti-6Al-4V alloys have been widely used in implant materials such as dental and orthopedic implants due to their corrosion resistance, biocompatibility, and good mechanical properties. However, surface modification of titanium and titanium alloys is necessary to improve osseointegration between implant surface and bone. Especially, when titanium oxide nanotubes are formed on the surface of titanium alloy, cell adhesion is greatly improved. In addition, plasma electrolytic oxide (PEO) coatings have a good safety for osseointegration and can easily and quickly form coatings of uniform thickness with various pore sizes. Recently, the effects of bone element such as magnesium, zinc, strontium, silicon, and manganese for bone regeneration are researching in dental implant field. The purpose of this study was researched on the surface morphology of PEO-treated Ti-6Al-4V alloy after anodic titanium oxide treatmentusing various instruments. Ti-6Al-4V ELI disks were used as specimens for nanotube formation and PEO-treatment. The solution for the nanotube formation experiment was 1 M $H_3PO_4$ + 0.8 wt. % NaF electrolyte was used. The applied potential was 30V for 1 hours. The PEO treatment was performed after removing the nanotubes by ultrasonics for 10 minutes. The PEO treatment after removal of the nanotubes was carried out in the $Ca(CH_3)_2{\cdot}H_2O+(CH_3COO)_2Mg{\cdot}4H_2O+Mn(CH_3COO)_2{\cdot}4H_2O+Zn(CH_3CO_2)_2Zn{\cdot}2H_2O+Sr(CH_2COO)_2{\cdot}0.5H_2O+C_3H_7CaO_6P$ and $Na_2SiO_3{\cdot}9H_2O$ electrolytes. And the PEO-treatment time and potential were 3 minutes at 280V. The morphology changes of the coatings on Ti-6Al-4V alloy surface were observed using FE-SEM, EDS, XRD, AFM, and scratch tester. The morphology of PEO-treated surface in 5 ion coating solution after nanotube removal showed formation or nano-sized mesh and micro-sized pores.

• The Journal of Korean Academy of Prosthodontics
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• v.44 no.3
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• pp.343-355
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• 2006

Statement of problem. The success of osseointegration can be enhanced with an implant that has improved surface characteristics. Anodic oxidation is one of the surface modifying method to achieve osseointegration. Voltage of anodic oxidation can change surface characteristics and cell activity Purpose. This study was performed to evaluate MG63 cell responses such as affinity, proliferation and to compare surface characteristics of anodic oxidized titanium in various voltage. Material and method. The disks for cell culture were fabricated from grade 3 commercially pure titanium,1 m in thickness and 12 mm in diameter. Surfaces of 4 different roughness were prepared. Group 1 had a machined surface, used as control. Group 2 was anodized under 220 V, group 3 was anodized under 300 V and group 4 was anodized under 320 V. The microtopography of specimens was observed by scanning electron microscope (JSM-840A, JEOL, Japan) and atomic force microscope(Autoprobe CP, Park Scientific Instrument, USA). The surface roughness was measured by confocal laser scanning microscope(Pascal, LSM5, Zeiss, Germany). The crystal structure of the titanium surface was analyzed with x-ray diffractometer(D8 advanced, Broker, Germany). MG63 osteoblast-like cells were cultured on these specimens. The cell morpholgy was observed by field emission electron microscope(Hitachi S-4700, Japan). The cell metabolic and proliferative activity was evaluated by MTT assay Results and conclusion. With in limitations of this in vitro study, the following conclusions were drawn. 1. In anodizing titanium surface, we could see pores which did not show in control group. In higher anodizing voltage, pore size was increased. 2. In anodizing titanium surface, we could see anatase. In higher anodizing voltage, thicker oxide layer increased crystallinity(anatase, anatase and rutile mixed). 3. MG63 cells showed more irregular, polarized and polygonal shape and developed more lamellipodi in anodizing group as voltage increased. 4. The activity of cells in MTT assay increased significantly in group 3 and 4 in comparison with group 1 and 2. However, there was no difference between group 3 and 4 at P<0.05. Proliferation of MG63 cells increased significantly in pore size($3-5.5{\mu}m$) of group 3 and 4 in comparison with in pore size($0.2-1{\mu}m$ ) of group 2.

• Journal of Dental Rehabilitation and Applied Science
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• v.30 no.2
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• pp.102-111
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• 2014

Purpose: Surface damage and bonding strength difference after micromechanical treatment of zirconia surface are to be studied yet. The aim of this study was to evaluate the difference of fracture resistance and bonding strength between more surface-damaged group from higher air-blasting particle size and pressure, and less damaged group. Materials and Methods: Disk shape zirconia ($LAVA^{TM}$) was sintered and air-blasted with $30{\mu}m$ particle size (Cojet), under 2.8 bar for 15 seconds, $110{\mu}m$ particle size (Rocatec), under 2.8 bar for 15 seconds, and $110{\mu}m$ particle size (Rocatec), under 3.8 bar for 30 seconds respectively. Biaxial flexure test and bonding failure load test were performed serially (n = 10 per group). For bonding test, specimens were bonded on the base material having similar modulus of elasticity of dentin with $200{\mu}m$-thick resin cement for tension of surface damage. Failure load of bonding was detected with acoustic emission (AE) sensor. Results: There were no significant differences both in the biaxial flexure test and bonding failure load test between groups (P > 0.05). Sub-surface cracks were all radial cracks except for two specimens. Conclusion: Within the limitations of no aging under monotonic load test, surface damage from higher air-blasting particle size and pressure was not significant. Evaluations of failure load with bonded zirconia disks was clinically relevant modality for surface damage and bonding strength, simultaneously.

• Journal of the Korea Society of Computer and Information
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• v.16 no.6
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• pp.21-30
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• 2011

In the global IT market, a lot of issues for responding to various environmental regulations emerged. In case of the data centers, it is consuming huge amounts of energy to maintain. So there have been various technical attempts as Consolidation, Virtualization, Optimization to efficiently manage energy and data storage to fix the problems. In this paper, we propose a new RAID(Redundant Array of Independent Disks) mechanism which is differing the intensity of power consumption and works to provide data protection and disaster recovery(backup, mirroring etc.) to stratify multiple volumes. G-RAID minimize the power consumption and the lower of I/O performance by selecting the volume depending on the frequency of data access while classifying the power consumption between volumes in storage system. Also, it is possible that a filesystem and block map information of G-RAID is processed by basic unit which is group located in a row for the blocks to work efficiently and can minimize the performance degradation of block mapping load by the access frequency in each groups. As a result, we obtained to elevate a little bit of response time caused by block relocation work, but showed the decrease of power consumption by 38%.

• Journal of Plant Biology
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• v.35 no.3
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• pp.197-203
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• 1992

During the study of the distribution of cellular slime molds in Halla mountain of Korea, a new yellow-pigmented Dictyostelium was isloated. This exibited several distinctive features which differed from the published species, and was designated as a new species, Dictyostelium jlavidum sp. n. Hong et Chang (Type strain HL-1). It was cultivated at $20-22^{\circ}C$ on weak nutrient agar media, 0.1 L-P in association with Escherichia coli. Sorocarps were 4-10 mm or more in length, conspicuously yellow throughout or with sori, typically solitary, unbranched or sparsely and irregularly branched. Sorophores were strongly tapered from bases to tips. Bases were typically well-formed disks in form or conically expanded. Sori were yellow to yellowish in color, and the pigmentation intensified with age. Spores were long and thin elliptical, mostly $4.8-9.6{\times}1.9-3.8\;(ave.\;7.3{\times}2.8)\;\mu\textrm{m}$, L/B index about 2.4-2.8, without polar granules.anules.