Background: Ingestion of $^{210}Po$ laden seafood accounts for a substantial amount of the effective dose of $^{210}Po$. Among seafood items, mollusks, especially domestically produced oysters and mussels, are highly enriched in $^{210}Po$ and are consumed in large quantities in Korea. Materials and Methods: Oysters and mussels around the Korean coasts were collected from major farm areas in November 2013. Samples were spiked with an aliquot of $^{209}Po$ as a yield tracer, and they were digested with $6mol{\cdot}L^{-1}$$HNO_3$ and $H_2O_2$. The $^{210}Po$ and $^{209}Po$ were spontaneously deposited onto a silver disc in an acidic solution of $0.5mol{\cdot}L^{-1}$ HCl and measured using an alpha spectrometer. The activity concentrations of $^{210}Pb$ and $^{210}Po$ were decay corrected to the sampling date, accounting for the possible in-growth and decay of $^{210}Po$. Results and Discussion: $^{210}Po$ activity concentrations in oysters were in a range from 41.3 to $206Bq{\cdot}(kg-ww)^{-1}$ and mussels in a range from 42.9 to $46.7Bq{\cdot}(kg-ww)^{-1}$. The $^{210}Po$ activity concentration of oysters in the turbid Western coast was higher than the Southern coast. The $^{210}Po$ activity concentration of the oysters was positively correlated ($R^2=0.89$) with those of the suspended particulate matter in the surface water. The calculated annual effective dose of $^{210}Po$ from oysters and mussels consumed by the Korean population was 21-104 and $5.01-5.46{\mu}Sv{\cdot}y^{-1}$. The combined effective dose due to the consumption of oysters and mussels appears to account for about $35{\pm}19%$ of that arising from seafood consumption in the Korean population. Conclusion: The annual effective dose of $^{210}Po$ for oysters in the Korean population was found to be higher than other countries. The total annual effective dose of $^{210}Po$ due to consumption of oysters and mussels consumed in Korea was found to be $76{\pm}42{\mu}Sv{\cdot}y^{-1}$, accounting for $28{\pm}16%$ of the total effective dose of $^{210}Po$ from food in Korea.
Journal of Dental Rehabilitation and Applied Science
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v.29
no.4
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pp.377-383
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2013
This study examined the radiopacity of eight contemporary luting cements by direct digital radiography. Five disc-shaped specimens ($5mm{\times}1mm$) were prepared for each material tested (BisCem, Clearfil SA Luting, Duolink, Maxcem Elite, Multilink Speed, Panavia F 2.0, RelyX Unicem Clicker, V-link). The specimens were radiographed using a Kodak CS 7600 image plate (Carestream Health, Inc., Rochester, NY, USA) and an aluminum step wedge with a range of thicknesses (1.5 to 16.5 mm in 1.5 mm increments) and a 1 mm tooth used as a reference. A dental X-ray machine Kodak 2200 Intraoral X-ray System (Carestream Health, Inc., Rochester, NY, USA), operating at 70 kVp, 4 mA, 0.156 s and a source-to-sample distance of 30 cm, was used. According to international standards, the radiopacity of the specimens was compared with that of an aluminum step wedge using NIH ImageJ software (available at http://rsb.info.nih.gov/ij/).The data was analyzed by ANOVA and a Tukey's post hoc test. Maxcem Elite (5.66) showed the highest radiopacity of all materials, followed in order by Multilink Speed (3.87) and V-link (2.83). The radiopacity of Clearfil SA Luting (1.35), BisCem (1.33), Panavia F 2.0 (1.29) and Duolink (1.10) were between enamel (1.79) and dentin (0.19). RelyX Unicem Clicker (0.71) showed the lowest radiopacity, which was higher than that of dentin. All materials showed a radiopacity above the minimum recommended by the International Organization for Standardization and the American National Standards/American Dental Association with the exception of RelyX Unicem Clicker.
Journal of the Korean Society of Food Science and Nutrition
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v.15
no.2
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pp.128-135
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1986
Wheat germ protein was extracted and isolated by a Modified Osborne fractionation method and some properties were investigated. The results are summarized as follows; 1. Approximate compositions of wheat germ were moisture 10.5%, crude protein 22.8%, crude fat 2.4%, crude ash 3.2%, crude fiber 1.5%, respectively. 2. Nitrogen solubilities on various solvents were the lowest as 45.58% by Osborne method and the highest as 79.49% after sequencial extraction of the $H_2O$, 0.5M-NaCl, 70%-ethanol, 0.1N-NaOH. 3. Isolated proteins yielded albumin, globulin, globulin, gliadin and glutelin in the proportion of 20.22: 17.49: 42.58: 19.71, respectively. 4. Spectrophometric chromatograms of isolated protein by Regel-filtration were two peaks in albumin (I ; 8.2%, II ; 91.8%), one peak in globulin (92.8%), respectively. 5. Disc-PAGE patterns were showed about 14 bands in 0.5M-Cl soluble protein, 3bands in crude albumin, 1band in main albumin, 2bands in crude globulin, one band in main globulin under pH 8.3 buffer system (Ornstein and Davis method).
The spot detection phase of the 2-DE image analysis program segments a gel image into spot regions by an image segmentation algorithm and fits the spot regions to a spot shape model and quantifies the spot informations for the next phases. Currently the watershed algorithm is generally used as the segmentation algorithm and there are the Gaussian model and the diffusion model for the shape model. The diffusion model is closer to real spot shapes than the Gaussian model however spots have very various shapes and especially an asymmetric formation in x-coordinate and y-coordinate. The reason for asymmetric formation of spots is known that a protein could not be diffused completely because the 2-DE could not be processed under the ideal environment usually. Accordingly we propose an asymmetric diffusion model in this paper. The asymmetric diffusion model assumes that a protein spot is diffused from a disc at initial time of diffusing process, but is diffused asymmetrically for x-axis and y-axis respectively as time goes on. In experiments we processed spot matching for 19 gel images by using three models respectively and evaluated averages of SNR for comparing three models. As averages of SNR we got 14.22dB for the Gaussian model, 20.72dB for the diffusion model and 22.85dB for the asymmetric diffusion model. By experimental results we could confirm the asymmetric diffusion model is more efficient and more adequate for spot matching than the Gaussian model and the diffusion model.
The purpose of this study was to evaluate the detection ability of secondary caries using qunatitative light-induce fluorescence-digital (QLF-D) device. Twenty bovine teeth with cavity on surface were demineralized during 21 days for secondary caries lesion of cavity wall. After 21 days, cavity was filled using composite resin and cut the specimen in half with disc. Fluorescence loss of lesion on surface by time flow, cross sectional lesion, and lesion of filled or unfilled surface were analyzed using analysis software. ${\Delta}F$ (value of fluorescence loss) of the lesion on surface assessed by the QLF-D increased significantly over time up to 21 days. And ${\Delta}F$ value of lesion of filled surface is significantly lower than that of unfilled surface (p<0.001). ${\Delta}F$ of filled surface is 1.31 times of cross section lesion. The correlation of between ${\Delta}F$ of filled surface lesion and ${\Delta}F$ of cross section lesion was showed low agreement (0.026) and correlation of between ${\Delta}F$ of unfilled surface lesion and ${\Delta}F$ of cross section lesion was showed high agreement (0.613). In conclusion, secondary caries can be detected on surface using QLF-D. However, interference of fluorescence of filling material is the points to be especially considered for exact analysis of secondary caries lesion.
Hyun, Ju Mi;Kim, Jung Eun;Yeum, Hyun Sook;Song, Jung-Min;Kim, Mi Ryang;Lee, Nam Ho
Journal of the Society of Cosmetic Scientists of Korea
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v.42
no.3
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pp.227-233
/
2016
This study was designed to analyze the chemical compositions of 'Setoka' branch essential oils (SEBO) and to test their biological activities. 'Setoka' is a Citrus species widely cultivated in Jeju Island. At the present, 'Setoka' branches produced by thinning process were mostly discarded as a waste. Therefore, utilization of this branch waste has received much attention. 'Setoka' branch essential oils (SBEO) were prepared by treatment of its ethanol extracts with jojoba oil. SBEO were chemically analyzed using gas chromatograph-mass spectrometry (GC-MS), and following components were identified; ethyl linoleate (64.14%), ethyl palmitate (16.50%), neophytadiene (11.06%) and beta-citronellol (5.09%). The anti-inflammatory activity in the SBEO was examined using RAW 264.7 murine macrophage cells stimulated with LPS. As a result, the SBEO inhibited nitric oxide (NO) productions with a dose-dependent manner. In addition, SBEO showed good anti-microbial activities against drug-susceptible and -resistant skin pathogens such as Staphylococcus aureus and Propionibacterium acnes, which are acne-causing bacteria. Based on these results, we suggest that SBEO has the possibility for use as an anti-inflammatory and anti-microbial agent in cosmetic applications.
Kim, Hyun-Soo;Ahn, Joung-Jwa;Choi, Tae-Ho;Hwang, Tae-Young
Food Science and Preservation
/
v.21
no.4
/
pp.593-599
/
2014
This study was conducted to determine the antioxidant potentials of some available plants to source alternate antioxidants and antibiotics. The antioxidant activity was evaluated by determining the antioxidant activity reducing powers and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities of extracts from some local edible native plants. The DPPH radical scavenging activity of the extracts was found to have been 0.41-94.84%. The Elsholtziasplendens NAKAI extract ($85^{\circ}C$, ethanol, accelerated solvent extraction) showed the highest level of antioxidant activity. Eight samples of plant extracts were evaluated for their antimicrobial activities against three microflora (Bacillus subtilis, Escherichia coli, and Candida albicans) using disc diffusion assay. Two samples (Geranium krameri FR. Et SAV. and Cleyera japonica Thunb.) had much more intensive antimicrobial activities than the control. However, their DPPH free radical scavenging activity levels were only 14.50 and 13.85, respectively. It is suggested that they could be used as natural preservatives against bacterial contamination in cosmetics and foods, in place of the common synthetic preservatives currently used.
Choi, Yong Seok;Hwang, In Su;Park, Deog Gee;Choe, Kwang Ryul
Korean journal of applied entomology
/
v.51
no.4
/
pp.343-348
/
2012
Developmental period, mummification, pupal mortality, and sex ratio of Korean strain of Aphelinus varipes F$\ddot{o}$rster parasitizing the green peach aphid, Myzus persicae were studied at 20, 25, 30, and $35^{\circ}C$ in controlled climate cabinets. The plastic container with a leaf disc of sweet pepper (Capsicum annuum L.) was used as experimental units. Green peach aphids at different developmental stages were reared in four different temperatures and presented to A. varipes for 12 hours. A female adult of A. varipes was allowed to parasitize for 12 hours in each plastic petri dish. The periods from egg to adult of A. varipes were 18.3 days, 14.7 days, 10.4 days, and 9.3 days at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The pupal mortalities of A. varipes were 0%, 1.5%, 2.8%, and 10.2% at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The mummifications of A. varipes were significantly different in all developmental stages of the aphid. The mummification of A. varipes parasitizing green peach aphid was the highest at 1st and 2nd in $20^{\circ}C$ and $25^{\circ}C$, but the highest at 3rd (84.4-85.0%) in $30^{\circ}C$ and $35^{\circ}C$. The host feedings of A. varipes were $20^{\circ}C$ (12.5%), $25^{\circ}C$ (17.3%), $30^{\circ}C$ (16%), and $35^{\circ}C$ (10.9%), respectively. The sex ratios of A. varipes female were $20^{\circ}C$ (49%), $25^{\circ}C$ (46%), $30^{\circ}C$ (69%), and $35^{\circ}C$ (60%), respectively.
The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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v.7
no.1
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pp.51-66
/
2001
Objective: The purpose of study was to compare physical therapy duration in relation tohealth care organization system in patients with low back pain. Subjects: Subjects of this study were 759 patients who are receiving physical therapy at 56 health care organization. Methods: Data were collected by questionnaire that had been completed by patients and physical therapist for two months. Physical therapy procedures consisted of modality application, manual therapy, active therapeutic exercise, and patient education. Physical therapy session duration was investigated for each physical therapy procedure. Data were analyzed in relation to the university hospital, hospital, and clinic. Results: The mean age of subjects was $42.84{\pm}15.46$. There were no significant differences in age among three groups. The number of patients diagnosed with herniated disc were 244(32.15%) and with mechanical low back pain was 187(24.64%). The mean treatment duration per day was 53.22 minutes, and the longest treatment duration was 61.28 minutes at the university hospitals (p<0.001). The mean modality application duration was 42.17 minutes, and the longest application duration was 46.26 minutes at the university hospitals (p<0.001). The mean duration for manual therapy was 5.11 minutes, and the longest treatment duration was 5.97 minutes at clinics. The mean duration for active therapeutic exercise was 4.16 minutes. It was 7.60 minutes at the university hospitals, and 2.48 minutes at clinics. There was a significant difference in active therapeutic exercise duration between university hospitals and clinics(p<0.001). For modalities, hot packs(89.33%) and interferential current therapy(60.87%) were mostly applied. For manual therapy, Soft tissue mobilization(32.93%) and manipulation(14.10%) were mostly applied. In general, treatment application duration was longer at University hospital (p<0.05). For therapeutic exercise, exercise without equipment(18.84%) and muscle strengthening (16.73%) were mostly performed. The longest treatment duration for therapeutic exercise was 7.60 minutes at the university hospital(p<0.05). Conclusion: physical therapy session duration for low back pain was 53.22 minutes. Modality application constitutes 79%, manual therapy 10%, active therapeutic exercise 8% of total treatment duration. It is concluded that patients do not participate actively in treatment procedures.
This study was to study the antibacterial effect of Lignum sappan extract on S. mutans growth. The antibacterial activities of Lignum sappan's crude extract was measured 17.3 mm at 20 mg/ml concentration. The growth of S. mutans in control medium was the highest at 8hr, while the media of Lignum sappan extract added-medium (2 mg/ml) showed maximum growth at 16hr. The pH values of the control media was 5.08 at 8hr, but the media supplemented with Lignum sappan extract was 6.69 at 8hr. The amounts of total carbohydrate of the control media was 0.81 mg/ml at 8hr, but the media supplemented with Lignum sappan extract was 2.06 mg/ml at 8hr. In the protein change of culture medium, the control culture broth and the cultures supplemented with Lignum sappan extract was 8.39 mg/ml and 12.3 mg/ml at 8hr, respectively. The S. mutans polysaccharide contents of the control media and the media supplemented with Lignum sappan extract was 300 mg/100ml and 60 mg/100ml at 8hr, respectively.
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