• Title/Summary/Keyword: disc

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Production and Identification of Secondary Metabolite Gliotoxin-Like Substance Using Clinical Isolates of Candida spp.

  • Noorulhuda Ojaimi Mahdi, Al-Dahlaki;Safaa Al-Deen Ahmed Shanter, Al-Qaysi
    • Microbiology and Biotechnology Letters
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    • v.50 no.4
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    • pp.488-500
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    • 2022
  • Most fungal infections by opportunistic yeast pathogens such as Candida spp. are the major causes of morbidity and mortality in patients with lowered immune. Previous studies have reported that some strains of Candida secret secondary metabolites play an important role in the decreasing of immunity in the infected patient. In this study, 110 Candida spp. were isolated from different clinical specimens from Baghdad hospitals. Candida isolates were identified by conventional methods, they were processed for Candida speciation on CHROMagar. The results of identification were confirmed by internal transcribed spacer (ITS) sequencing. Phylogenetic trees were analyzed with reference strains deposited in GenBank. Antifungal susceptibility testing was evaluated by the disc diffusion method and performed as recommended by the Clinical and Laboratory Standard Institute (CLSI) M44-A document. Candida isolates investigated produce secondary metabolites gliotoxin with HPLC technique and quantification. Out of 110 Candida isolates, C. albicans (66.36%) was the most frequent isolate, followed by the isolates of C. tropicalis (10.9%) and C. glabrata (6.36%) respectively. Concerning the antifungal susceptibility test, Candida isolates showed a high level of susceptibility to Miconazole (70.9%), Itraconazole (68.2%), and Nystatine (64.5%). The ability of obtained isolates of Candida spp. to produce gliotoxin on RPMI medium was investigated, only 28 isolates had the ability to secret this toxin in culture filtrates. The highest concentrations were detected in C. albicans (1.048 ㎍/ml). Gliotoxin productivity of other Candida species was significantly lower. The retention time for gliotoxin was approximately 5.08 min.

Seismic retrofit of steel structures with re-centering friction devices using genetic algorithm and artificial neural network

  • Mohamed Noureldin;Masoum M. Gharagoz;Jinkoo Kim
    • Steel and Composite Structures
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    • v.47 no.2
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    • pp.167-184
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    • 2023
  • In this study, a new recentering friction device (RFD) to retrofit steel moment frame structures is introduced. The device provides both self-centering and energy dissipation capabilities for the retrofitted structure. A hybrid performance-based seismic design procedure considering multiple limit states is proposed for designing the device and the retrofitted structure. The design of the RFD is achieved by modifying the conventional performance-based seismic design (PBSD) procedure using computational intelligence techniques, namely, genetic algorithm (GA) and artificial neural network (ANN). Numerous nonlinear time-history response analyses (NLTHAs) are conducted on multi-degree of freedom (MDOF) and single-degree of freedom (SDOF) systems to train and validate the ANN to achieve high prediction accuracy. The proposed procedure and the new RFD are assessed using 2D and 3D models globally and locally. Globally, the effectiveness of the proposed device is assessed by conducting NLTHAs to check the maximum inter-story drift ratio (MIDR). Seismic fragilities of the retrofitted models are investigated by constructing fragility curves of the models for different limit states. After that, seismic life cycle cost (LCC) is estimated for the models with and without the retrofit. Locally, the stress concentration at the contact point of the RFD and the existing steel frame is checked being within acceptable limits using finite element modeling (FEM). The RFD showed its effectiveness in minimizing MIDR and eliminating residual drift for low to mid-rise steel frames models tested. GA and ANN proved to be crucial integrated parts in the modified PBSD to achieve the required seismic performance at different limit states with reasonable computational cost. ANN showed a very high prediction accuracy for transformation between MDOF and SDOF systems. Also, the proposed retrofit showed its efficiency in enhancing the seismic fragility and reducing the LCC significantly compared to the un-retrofitted models.

Comparative Study on the Clinical Trial of Acupotomy Treatment for Lumbar Disk Herniation

  • Beom Seok Kim;Ju Hyun Jeon;Tae Yong Park;Hong Kyoung Kim;Young Il Kim
    • Journal of Acupuncture Research
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    • v.40 no.3
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    • pp.252-264
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    • 2023
  • Background: The primary aim of this study is to statistically analyze and compare the difference in treatment outcomes based on the frequency and duration of acupotomy treatment for lumbar disk herniation. Methods: The evaluation of efficacy and safety involved using visual analog scale (VAS), numeric rating scale (NRS), Roland Morris disability questionnaire (RMDQ), EuroQol 5-dimention (EQ-5D), and similar parameters. This was achieved through the establishment of a comparable cohort using propensity score matching. Group A comprised 46 patients who underwent 4 sessions of acupotomy treatment for 2 weeks, while Group B consisted of 15 patients who received 6 acupotomy sessions for 3 weeks. Results: For Group A, the average amount of change in VAS values at weeks 4 and 6 was significantly decreased. However, the average amount of change in RMDQ values was not significant. Notably, the average amount of change of EQ-5D values at week 6 increased significantly. As for Group B, the average amount of change of NRS values in weeks 2 and 3 was significantly decreased. Conversely, the average amount of change in RMDQ and EQ-5D values was not significant. In comparing the 2 groups at the final follow-up, the average amount of change in all evaluation values was not significant. Further, no significant interaction effect was observed in the changes over time in all evaluation values between the 2 groups. Conclusion: This study establishes that there was no statistically significant difference in pain reduction, functional impairment, and quality of life improvement between 2 group.

AlInGaN - based multiple quantum well laser diodes for Blu-ray Disc application

  • O. H. Nam;K. H. Ha;J. S. Kwak;Lee, S.N.;Park, K.K.;T. H. Chang;S. H. Chae;Lee, W.S.;Y. J. Sung;Paek H.S.;Chae J.H.;Sakong T.;Kim, Y.;Park, Y.
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.11a
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    • pp.20-20
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    • 2003
  • We developed 30 ㎽-AlInGaN based violet laser diodes. The fabrication procedures of the laser diodes are described as follows. Firstly, GaN layers having very low defect density were grown on sapphire substrates by lateral epitaxial overgrowth method. The typical dislocation density was about 1-3$\times$10$^{6}$ /$\textrm{cm}^2$ at the wing region. Secondly, AlInGaN laser structures were grown on LEO-GaN/sapphire substrates by MOCVD. UV activation method, instead of conventional annealing, was conducted to achieve good p-type conduction. Thirdly, ridge stripe laser structures were fabricated. The cavity mirrors were formed by cleaving method. Three pairs of SiO$_2$ and TiO$_2$ layers were deposited on the rear facet for mirror coating. Lastly, laser diode chips were mounted on AlN submount wafers by epi-down bonding method. The lifetime of the laser diodes was over 10,000 hrs at room temperature under automatic power controlled condition. We expect the performance of the LDs to be improved by the optimization of the growth and fabrication process. The detailed characteristics and important issues of the laser diodes will be discussed at the conference.

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A Study on the Microstructures and Ionic Conductivity of Li1.3Al0.3Ti1.7(PO4)3 with Different Synthesis Routes (합성 방법에 따른 Li1.3Al0.3Ti1.7(PO4)3 소결체의 미세 구조 및 이온전도 특성 연구)

  • Seul Ki Choi;Jeawon Choi;MinHo Yang
    • Journal of Powder Materials
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    • v.30 no.2
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    • pp.107-115
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    • 2023
  • Li1.3Al0.3Ti1.7(PO4)3(LATP) is considered a promising material for all-solid-state lithium batteries owing to its high moisture stability, wide potential window (~6 V), and relatively high ion conductivity (10-3-10-4 S/cm). Solid electrolytes based on LATP are manufactured via sintering, using LATP powder as the starting material. The properties of the starting materials depend on the synthesis conditions, which affect the microstructure and ionic conductivity of the solid electrolytes. In this study, we synthesize the LATP powder using sol-gel and co-precipitation methods and characterize the physical properties of powder, such as size, shape, and crystallinity. In addition, we have prepared a disc-shaped LATP solid electrolyte using LATP powder as the starting material. In addition, X-ray diffraction, scanning electron microscopy, and electrochemical impedance spectroscopic measurements are conducted to analyze the grain size, microstructures, and ion conduction properties. These results indicate that the synthesis conditions of the powder are a crucial factor in creating microstructures and affecting the conduction properties of lithium ions in solid electrolytes.

Effect of orthodontic bonding with different surface treatments on color stability and translucency of full cubic stabilized zirconia after coffee thermocycling

  • Yasamin Babaee Hemmati;Hamid Neshandar Asli;Alireza Mahmoudi Nahavandi;Nika Safari;Mehran Falahchai
    • The korean journal of orthodontics
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    • v.53 no.3
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    • pp.139-149
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    • 2023
  • Objective: To assess the color stability and translucency of full cubic stabilized zirconia (FSZ) following orthodontic bonding with different surface treatments and coffee thermocycling (CTC). Methods: This in vitro study was conducted on 120 disc-shaped specimens of FSZ. Thirty specimens were selected as the control group and remained intact. The remaining specimens were randomly divided into three groups based on the type of surface treatment (n = 30): airborne particle abrasion (APA), silica-coating (CoJet), and carbon dioxide (CO2) laser. After metal bracket bonding in the test groups, debonding and polishing were performed. Subsequently, all specimens underwent CTC (10,000 cycles). Color parameters, color difference (ΔE00), and translucency parameter (TP) were measured three times at baseline (t0), after debonding and polishing (t1), and after CTC (t2). Data were statistically analyzed (α = 0.05). Results: Significant difference existed among the groups regarding ΔE00t0t2 (p < 0.001). The APA group showed minimum (ΔE00 = 1.15 ± 0.53) and the control group showed maximum (ΔE00 = 0.19 ± 0.02) color stability, with no significant difference between the laser and CoJet groups (p = 0.511). The four groups were significantly different regarding ΔTPt0t2 (p < 0.001). Maximal increases in TP were noted in the CoJet (1.00 ± 0.18) and APA (1.04 ± 0.38) groups while minimal increase was recorded in the control group (0.1 ± 0.02). Conclusions: Orthodontic treatment makes zirconia restorations susceptible to discoloration and increased translucency. Nonetheless, the recorded ΔE00 and ΔTP did not exceed the acceptability threshold.

Assaying of SNM using Simultaneous Detection of Fission Neutrons and Gammas by Employing a Novel Phoswich Detector

  • Sonu;Mohit Tyagi;A. Kelkar;A. Sahu;M. Sonawane;P.S. Sarkar;A. Pandey;D.B. Sathe;G.D. Patra;T. Vincent;S.G. Singh;R.B. Bhatt
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2662-2669
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    • 2023
  • For the precise measurements of special nuclear materials (SNM) including Pu and Am isotopes, we have used phoswich detector combination of two single crystal scintillators of Gd3Ga3Al2O12:Ce and CsI:Tl. High detection efficiency and sensitivity along with high figure of merit for the discrimination of these phoswich detectors ensures the detection and discrimination of thermal neutrons and gammas from spontaneous fission of Pu and other isotopes in presence of high gamma background. Using this detector, the low energy gammas, which is stopped completely in 1mm thick disc of GGAG, can be also discriminated from high energies gamma and shows linearity in wide range of sample quantities. By changing only the appropriate shielding, the similar setup was used for thermal neutron detection and shows a very good linearity over wide range. The quantity of a test sample was also calculated accurately by using the measured calibrated plot.

Relationships between the Host and it's Natural Enemy Introduced into a New Ecosystem 2. Effect of Ingestion by Podisus maculiventris Say on the Prey Size of Hyphantria cunea Drury (生態系內에서의 宿主와 天敵의 數的變動에 따른 相關關係에 關한 硏究: 2. 미국흰불나방 크기에 따른 Podisus maculiventris의 捕食劾果에 關한 硏究)

  • Kim, Chang-Whan;Noh, Yong Tai;Chung, Young Wha
    • The Korean Journal of Zoology
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    • v.12 no.4
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    • pp.109-113
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    • 1969
  • Exeriments were carried out to determine the effect of age on the functional response of the predator, Podisus maculiventris reared on the different prey size of Hyphantria cunea in simple vial, twice at the 1st and 2nd generations of Hyphantria cunea. 1. Larvae ingested about 11.23, 18.4 and 20.7mg for each diet level (cf, table 1 to 3)in 2nd instar, 12.3, 23.37 and 28.3mg in 3rd instar, 18.17, 40.3 and 42.7mg in 4th instar, 51.7, 92.7 and 130.2mg in 5th instar for each diet level. 2. Adult males ingested about 528, 740, 545.1mg and females about 516.5, 627.5, 603.3mg in their whole life for each diet level. 3. The relationship between observed and predicted values, established by regression analysis was revealed as 0.80 to 0.91 in correlation coefficient.

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The Effects of Incised Meandering Valley and Lithological Differences on the Grain Size and Shape of Channel Bed Materials: A Case Study of the Upper and Middle Reaches of Gongneungcheon River (감입곡류 지형과 암질 차이가 하상 퇴적물 입경 및 형상에 미친 영향: 공릉천 중상류 구간을 사례로)

  • Chen, Hui;Kim, Jong Wook;Han, Min;Byun, Jongmin
    • Journal of The Geomorphological Association of Korea
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    • v.26 no.1
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    • pp.15-26
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    • 2019
  • In this study, we investigated the grain size, lithological type, and shape of coarse bed materials in the upper and middle reaches of Gongneungcheon River. For this purpose, 11 sampling points were selected along the river. For 100 samples of the coarse bed materials at each point, three axes (long, intermediate, and short) of samples were measured, and their lithological types were also identified. By measuring grain size, the sphericity and flatness of samples were calculated. Finally, every particle was classified into four shape categories: sphere, disc, blade and rod. We found that the grain size in incised meandering reach is the largest. This is mainly due to the supply of coarse materials from steep valley sides along the meandering channel. According to the lithological analysis, all samples were identified as granite, gneiss and schist, and quartz. The proportion of granite decreased, whereas the proportion of gneiss and schist increased downstream. These patterns indicate that the bedrock distribution within the study area accounts for the downstream lithological variation of coarse bed materials. With regard to the grain shape, sphericity gradually decreased while flatness gradually increased downstream. In the case of the shape classification, unlike the general downstream pattern of grain shape, the proportion of the sphere type decreased and the proportion of the blade type increased downstream. Such a reversal change in the downstream direction turns out to be determined by the lithology (such as foliation, bedding and the pattern of weathering) of coarse bed materials.

Surface gloss, gloss retention, and color stability of 2 nano-filled universal resin composites

  • Gustavo Fabian Molina;Ricardo Juan Cabral;Ignacio Mazzola;Michael Burrow
    • Restorative Dentistry and Endodontics
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    • v.47 no.4
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    • pp.43.1-43.13
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    • 2022
  • Objectives: This study compared the surface gloss (SG), gloss retention (GR), and color stability (CS) of 2 universal resin composites after chemical (CA) and mechanical (MA) aging. Materials and Methods: Twenty disc-shaped samples of G-ænial A'Chord (GC-Europe) and Filtek Universal (3M-ESPE) were polished with sequential abrasive papers. For CA, specimens were stored in 1 mL of 75% ethanol for 15 days at 37℃, and readings (SG, GR, and CS) were obtained at baseline and 5, 10, and 15 days. For MA, specimens were subjected to 10,750 simulated brushing cycles. SG and CS were evaluated after every 3,583 cycles. SG was measured with a glossmeter (geometrical configuration: 60°), and values were expressed in gloss units. Color was measured with a spectrophotometer using the CIE-L*a*b* color system. The Student's t-test, 1-way analysis of variance, and Scheffé test were used for statistical analysis (α = 0.05). Results: G-ænial presented significantly higher SG values than Filtek (p = 0.02), with GR reductions of 5.2% (CA) and 5.3% (MA) for G-ænial and 7.6% (CA) and 7.2% (MA) for Filtek. The aging protocol had no statistically significant effect on SG or GR (p = 0.25) from baseline to the final readings. G-ænial-MA presented the lowest color difference (ΔE = 1.8), and G-ænial-CA and Filtek-CA had the largest changes (ΔE = 8.6 and ΔE = 11.8, respectively). Conclusion: G-ænial presented higher SG values and better CS. Both restorative materials demonstrated acceptable GR and CS. Aging protocols impacted these properties negatively.