• Korean Journal of Weed Science
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• v.9 no.3
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• pp.214-220
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• 1989

The experiments were conducted to evaluate safening effect of fenclorim (CGA 123'407, 4,6-dichloro-2-phenyl pyrimidine) against injuries of pretilachlor, butachlor, and benthiocarb in direct-seeded rice under flooded conditions. Pretilachlor and butachlor and benthiocarb reduced crop standing and inhibited growth of rice plants. However, premixture application of pretilachlor, butachlor or benthiocarb with fenclorim at 10-30 g ai/10a greatly reduced rice injury caused by the application of each herbicide alone and thus increased crop standing plant height and dry weight of rice plants compared to herbicide treatments alone.

• Korean Journal of Weed Science
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• v.16 no.1
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• pp.28-35
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• 1996

Tetrapion(2,2,3,3-tetrafluoropropionate sodium) has commonly been used for reed(Phragmites communis Trin.) control in uncultivated areas, especially in Japan. As an attempt to establish the screening system for selective herbicide controlling reed, tetrapion was tested as a standard herbicide to various weeds and crops including rice in a greenhouse. Symptoms of yellowing, twisting, stunting, and necrosis were observed in the herbicide-treated plants. The herbicide caused a severe damage on all crops examined, except cotton. Both direct seeded and transplanted rice were also sensitive to the herbicide. Its herbicidal activity was generally higher on grass weeds than on broadleaf weeds. It had a higher herbicidal activity with preemergence treatment than with postemergence treatment. In addition, field and greenhouse experiments were conducted to examine the effect of the herbicide on reed control. Its effect varied with the soil type of the treated field and the growth stage of reed. A relatively high dosage of the herbicide was required to control reed. It had an almost same effect on fairygrass(Miscanthus sinensis Anderss) as on reed. However, reed grown in a greenhouse was effectively controlled by the herbicide, having more effect with preemergence treatment than with postemergence treatment as observed in other plants treated with the herbicide. In conclusion, tetrapion has a great potential as a standard herbicide during the herbicide screening for selective reed control.

• Korean Journal of Weed Science
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• v.12 no.2
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• pp.183-187
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• 1992

This study was conducted to evaluate the effect of systematic herbicide application and polyethylene(PE) film mulching in direct seeded Angelica gigas Nakai. Freshly-collected-seed showed 86% of emergence rate and required 18 days of emergence duration. Cold treatment of seeds increased emergence rate by more than 90% and shortened 2 days of emergence duration. Paraquat) 1,1'-dimethyl-4,4'-bipyridylium dichloride was the most effective when applied just before emergence of A. gigas and provided 96% of emergence rate and 91% of weeding effect when applied 23 days after seeding. Soil applied herbicides used did not cause any phytotoxicity on emergence with application of 2-fold recommended rate and gave more than 90% of weeding effect. Black PE film mulching gave excellent emergence rate and weeding effect, and shortened emergence duration by 8 days.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.2
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• pp.154-159
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• 1991

A field experiment was conducted to know the feasibility of direct-seeded rice. Dry seeds of a Japonica type rice variety, Donghaebyeo, was sown from May 10 to June 20 at the 10-day intervals. The number of days from seeding to emergence decreased as planting date delayed; 13, 12, 10, 9, and 7 days at May 10, May 20. May 30. and June 10 seedings, respectively. The number of maximum tillers per ㎡ was about 700-800 at all the seeding dates and effective tiller ratio ranged 40-45%. The number of days from seeding to heading decreased as seeding date delayed; from 97 days at May 10 planting to 71 days at June 20 planting. The cumulative effective temperature (mean air temperature-15$^{\circ}C$) from seeding to heading was fairly constant having 870$^{\circ}C$ with 2.3% C.V. The number of panicles per unit area at June 10 planting was higher than that of other planting dates. Panicle length and the number of spikelets per panicle tended to decrease as seeding date delayed. The number of spikelets per unit area, 1000-grain weight, and grain yield were similar among the seeding dates except June 20 seeding. At the June 20 seeding the number of spikelets and grain yield were much lower, but 1000-grain weight was higher compared with other seeding dates. Grain fertility and percent ripened grains was similar among all seeding dates.

• Korean Journal of Weed Science
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• v.15 no.2
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• pp.105-114
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• 1995

Differences in critical period of weed competition were investigated among five types of rice(Oryza sativa L.) cultivation. Increase in weed-free period resulted in 1-2 days delay of heading date in machine transplanting and direct-seeding as compared with complete weed-free plot. When weedy period increased, there was no difference in heading date in transplanting cultivations. In direct-seeding, however, weedy period of 7-10 weeks after seeding(WAS) resulted in 4-7 days delay of heading date, whereas further increase in the weedy period caused rather 5-6 days advance in heading date. Weed-free period did not significantly affect yield components in conventional hand transplanting. In machine transplanting with 30-day-old seedling decreases in percent ripening and 1,000-grain weight were caused by weeds emerged within 4 weeks after transplanting(WAT). All yield components were decreased due to weedy period in machine transplanting with 10-day-old seedling. In direct-seeding weedy periods caused to decrease in number of panicles, number of spikelets, percent ripening, and 1,000-grain weight were 8-9, 4-5, 3-4, and 8-10 WAS, respectively. The critical periods of weed competition were determined as the following. In conventional hand transplanting weed-free must be maintained for either 4 weeks after transplanting or the rest period after 8 WAT. In machine transplanting with 30-day-old seedling weed-free must keep for either 5 weeks after transplanting or the rest period after 8 WAT. In machine transplanting with 10-day-old seedling weeds must be removed for either 5 weeks after transplanting or the rest period after 7 WAT. Weed-free must be kept between 5 and 7 WAS in flood direct-seeded rice and between 6 and 9 WAS in dry direct-seeded rice.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.1
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• pp.5-18
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• 2018

The field trial was performed to evaluate the rice growth and yield in direct seeding cultivation with iron-coated rice seeds. The required time for seed emergence was for 9~11days in the tested direct seeding methods. That was 1~2days earlier in direct seeding with pregerminated seeds than that of direct seeding with iron-coated seeds. The seedling establishment was highest in water seeding with iron-coated seeds but there was not significant difference in terms of statistical analysis. The rice plant height was taller in water seeding with broadcasting method than that of wet hill-seeding methods and in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The tiller number in the rice plant was the highest in machine transplanting at 30days after direct seeding(June 17) and in water seeding with iron-coated seeds at 45days after seeding(DAS) and 60DAS. The tiller number of 75 and 90DAS in the tested rice cultivation methods being with 352~405/m² was not significantly different in terms of statistical analysis. The heading time was not different in rice direct seeding methods but 2 day earlier in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The culm length was the highest in water seeding with iron-coated seeds and the panicle length was the longest in wet hill-seeding with pregerminated seeds. The panicle number per m² was highest in water seeding with iron-coated seeds but not significant difference among the tested rice cultivation methods. The water seeding with iron-coated seeds resulted in the highest spikelet number per m² and the heaviest grain weight of brown rice. Percentage of ripened kernel was the highest in wet hill-seeding with iron-coated seeds. But there were not significant among the tested rice cultivation methods. The milled rice yield in direct seeding methods was 3~21% higher than that in machine transplanting. Water seeding with iron-coated seeds recorded the highest milled rice yield being with 6.86t/ha.The occurrence of sheath blight was high according to machine transplanting>wet hill-seeding>water seeding. Weed occurrence was the highest in water seeding with pregerminated seeds. Weedy rice occurred not in machine transplanting but occured 0.6~0.7% in direct seeding methods with pregerminated seeds and 0.1% in direct seeding with iron-coated seeds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.2
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• pp.97-106
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• 1991

Two dimensional non-parametric model using daily mean temperature and daylength as predictor variables was established and daily developmental rates (DVR) for the period of seedling emergence to heading were estimated for 26 rice cultivars by using data from field direct seeding dates and short-day treatments experiment carried out at experimental farm of Seoul National University in 1990. Three existing parametric models were tested for the comparision of predictability with non-parametric model. The non-parametric model was found to be superior to parametric models in predicting heading date. The developmetal indice(DVI) at heading date, cummulative DVR's from seedling emergence showed 0.5 to 2.2 percent of coefficient of variations. The non-parametric model revealed errors of 0 to three days in 11 varieties when applied to data independent of those used in estimating DVR.

• Korean Journal Plant Pathology
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• v.11 no.1
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• pp.94-97
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• 1995

In 1994, an abnormal rice growth was observed in a 0.5-ha scale farmer's field located at Chogye-Myeon, Hapcheon-Gun, Gyeongnam Province where rice cv. Hwayeongbyeo was seeded directly in dry paddy field conditions. The major symptoms were less stands per acreage, short leaf length, and hypertrophy of root tip portion. The disorder was identified as rice seeding bight caused by Rhizopus sp. The average plant stand per m2 in the infested field was 108, while it was 375 in the normal field, and leaf growth was retarded to less than one-third of healthy plant. The average leaf lengths of infected and healthy seedlings were 12.8cm and 38.9cm, respectively. When the infected seedlings were transplanted to pots, flooded-soil conditions developed no new root growth but upland conditions allowed 11.2 new roots emerging. The length of newly emerged root in infested soil was 5.3cm in upland and 7.1cm in intermediate flooding conditions. However, it was 10.9cm in non-infested soil with intermediate flooding conditions. When the plants were matured, the stem length of infected plants was reduced slightly as compared to normal plants, whereas the length of panicle was not significantly different between infected and healthy plants. The number of panicle per plant, however, greatly different with variation of infection degree. Grain quality such as the number of complete and incomplete grains per panicle, the complete grain weight per panicle, and the weight of 1000 grains was not significantly different.

• International Journal of Stem Cells
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• v.16 no.2
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• pp.244-249
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• 2023

Background and Objectives: To examine whether ischemic retinal ganglion cells (RGCs) will be salvaged from cell death by human adipose-derived mesenchymal stem cells (ADSCs) in an organotypic retina model. Methods and Results: Deprived of arterial oxygen supply, whole mice retinas were cultured as an ex vivo organotypic cultures on an insert membrane in a 24-well plate. The therapeutic potential of ADSCs was examined by co-culture with organotypic retinas. ADSCs were seeded on top of the RGCs allowing direct contact, or at the bottom of the well, sharing the same culture media and allowing a paracrine activity. The number of surviving RGCs was assessed using Brn3a staining and confocal microscopy. Cytokine secretion of ADSCs to medium was analyzed by cytokine array. When co-cultured with ADSCs, the number of surviving RGCs was similarly significantly higher in both treatment groups compared to controls. Analysis of ADSCs cytokines secretion profile, showed secretion of anti-apoptotic and pro-proliferative cytokines (threshold>1.4). Transplantation of ADSCs in a co-culture system with organotypic ischemic retinas resulted in RGCs recovery. Since there was no advantage to direct contact of ADSCs with RGCs, the beneficial effect seen may be related to paracrine activity of ADSCs. Conclusions: These data correlated with secretion profile of ADSCs' anti-apoptotic and pro-proliferative cytokines.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.134.1-134.1
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• 2017

In this study, we propose a new scaffold fabrication method, "direct electro-hydrodynamic jet process," using the initial jet of an electrospinning process and ethanol media as a target. The fabricated threedimensional (3D) fibrous structure was configured with multilayered microsized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes. The fabrication of the structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous scaffold was cultured with preosteoblasts to observe the initial cellular activities in comparison with a solid-freeform fabricated 3D scaffold sharing a similar structural geometry. The cell-culture results showed that the newly developed scaffold provided outstanding microcellular environmental conditions to the seeded cells (about 3.5-fold better initial cell attachment and 2.1-fold better cell proliferation).