• Title/Summary/Keyword: direct system

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A Simple and Rapid Gene Amplification from Arabidopsis Leaves Using AnyDirect System

  • Yang, Young-Geun;Kim, Jong-Yeol;Soh, Moon-Soo;Kim, Doo-Sik
    • BMB Reports
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    • 제40권3호
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    • pp.444-447
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    • 2007
  • Polymerase chain reaction (PCR) is a powerful technique in molecular biology and is widely used in various fields. By amplifying DNA fragments, PCR has facilitated gene cloning procedures, as well as molecular genotyping. However, the extraction of DNA from samples often acts as a limiting step of these reactions. In particular, the extraction of PCR-compatible genomic DNA from higher plants requires complicated processes and tedious work because plant cells have rigid cell walls and contain various endogenous PCR inhibitors, including polyphenolic compounds. We recently developed a novel solution, referred to as AnyDirect, which can amplify target DNA fragments directly from whole blood without the need for DNA extraction. Here, we developed a simple lysis system that could produce an appropriate template for direct PCR with AnyDirect PCR buffer, making possible the direct amplification of DNA fragments from plant leaves. Thus, our experimental procedure provides a simple, convenient, non-hazardous, inexpensive, and rapid process for the amplification of DNA from plant tissue.

DirectX를 이용한 원자력발전소 증기발생기 슬러지 제거용 로봇작업의 3차원 Visualization 시스템 개발 (Development of 3d visualization system of sludge lancing system for $\Delta$60 steam generator using directX)

  • 김석곤
    • 전력전자학회:학술대회논문집
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    • 전력전자학회 2000년도 전력전자학술대회 논문집
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    • pp.476-479
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    • 2000
  • 한국전력연구원은 로봇제어기술과 가상현실기술을 접목하는 과정의 일환으로 로봇작업을 3D 그래픽으로 가시화(Visualization) 하는 연구를 수행하고 있다 본 논문에서는 원자력발전소 증기발생기 내부에 발생하는 슬러지를 제거하기 위한 로봇시스템과 그 작업내용의 3차원 Visualization 시스템 개발에 관한 내용을 다룬다. 본 시스템은 Microsoft사의 제작툴인 DirectX를 그래픽엔진으로 사용하였으며 C++로 작성되어있어 매우 개방적인 구조를 가지고 있어 타 분야로의 적용이 용이하다.

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직접 STR을 이용한 웹 이송 시스템에서의 장력제어 (Tension Control in Web Transport System using Direct Self-tuning Regulator)

  • 오기석;권태종;한창수;강남기;조형진
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 1996년도 추계학술대회 논문집
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    • pp.236-242
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    • 1996
  • The purpose of this paper is to study the tension control in a web transport system. Direct self-tuning regulator method was applied to tension controller and variable-gain PID control algorithm was applied to web speed controller. The designed controllers compensated for the time-varying parameters and tracked reference tension in process speed changing. The simulation shows that direct STR tension controller improves tension control performance in comparison with other controllers.

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DSP로 구현된 유도 전동기의 직접토크 제어 (Direct Torque Control of Induction motor using DSP)

  • 곽진표;박진홍;김태웅;이성백
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2000년도 하계학술대회 논문집 B
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    • pp.1033-1035
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    • 2000
  • This paper presents and analysis a system control algorithm of direct torque control for induction motor using digital signal professor. Using direct torque control, robust. simple and dynamic motor control can be realized. The implemented system is verified by analyzing control torque and flux characteristic.

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디지털 방사선영상 시스템의 기본적 원리 (Physical principles of digital radiographic imaging system)

  • 최진우;이원진
    • Imaging Science in Dentistry
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    • 제40권4호
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    • pp.155-158
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    • 2010
  • Digital radiographic systems allow the implementation of a fully digital picture archiving and communication system (PACS), and provide the greater dynamic range of digital detectors with possible reduction of X-ray exposure to the patient. This article reviewed the basic physical principles of digital radiographic imaging system in dental clinics generally. Digital radiography can be divided into computed radiography (CR) and direct radiography (DR). CR systems acquire digital images using phosphor storage plates (PSP) with a separate image readout process. On the other hand, DR systems convert X-rays into electrical charges by means of a direct readout process. DR systems can be further divided into direct and indirect conversion systems depending on the type of X-ray conversion. While a direct conversion requires a photoconductor that converts X-ray photons into electrical charges directly, in an indirect conversion, lightsensitive sensors such as CCD or a flat-panel detector convert visible light, proportional to the incident X-ray energy by a scintillator, into electrical charges. Indirect conversion sensors using CCD or CMOS without lens-coupling are used in intraoral radiography. CR system using PSP is mainly used in extraoral radiographic system and a linear array CCD or CR sensors, in panoramic system. Currently, the digital radiographic system is an important subject in the dental field. Most studies reported that no significant difference in diagnostic performance was found between the digital and conventional systems. To accept advances in technology and utilize benefits provided by the systems, the continuous feedback between doctors and manufacturers is essential.

Direct metal laser sintering 방식을 이용하여 제작한 다양한 고정성 보철물 수복 증례 (Fixed prostheses fabricated by direct metal laser sintering system: case report)

  • 백주원;신수연
    • 구강회복응용과학지
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    • 제32권3호
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    • pp.246-254
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    • 2016
  • Direct metal laser sintering (DMLS) 방식은 3D 프린팅 중 한 방식으로, 재료를 쌓아가면서 레이저를 이용하여 선택적으로 sintering하는 방식이다. 이는 주조 방식에서 문제되는 결손과 뒤틀림을 방지할 수 있으며 절삭 가공 방식으로 제작하기 어려운 복잡한 구조물을 제작할 수 있다. 본 증례들은 DMLS 방식을 이용하여 지대치 간 길이가 긴 고정성 보철물, 포스트 등 다양한 고정성 보철물을 제작하여 수복하였고, 주기적인 관찰 결과 심미적, 기능적으로 만족할만한 결과를 얻었기에 이를 보고하는 바이다.

An Experimental Study on the Performance of Air/Water Direct Contact Air Conditioning System

  • Yoo, Seong-Yeon;Kwon, Hwa-Kil
    • Journal of Mechanical Science and Technology
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    • 제18권6호
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    • pp.1002-1009
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    • 2004
  • Direct contact air conditioning systems, in which heat and mass are transferred directly between air and water droplets, have many advantages over conventional indirect contact systems. The purpose of this research is to investigate the cooling and heating performances of direct contact air conditioning system for various inlet parameters such as air velocity, air temperature, water flow rate and water temperature. The experimental apparatus comprises a wind tunnel, water spray system, scrubber, demister, heater, refrigerator, flow and temperature controller, and data acquisition system. The inlet and outlet conditions of air and water are measured when the air contacts directly with water droplets as a counter flow in the spray section of the wind tunnel, and the heat and mass transfer rates between air and water are calculated. The droplet size of the water sprays is also measured using a Malvern Particle Analyzer. In the cooling conditions, the outlet air temperature and humidity ratio decrease as the water flow rate increases and as the water temperature, air velocity and temperature decrease. On the contrary, the outlet air temperature and humidity ratio increase in the heating conditions as the water flow rate and temperature increase and as the air velocity decreases.

Accuracy Comparison of Direct Georeferencing and Indirect Georeferencing in the Mobile Mapping System

  • Bae Sang-Keun;Kim Byung-Guk;Sung Jung-Gon
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2004년도 Proceedings of ISRS 2004
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    • pp.656-660
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    • 2004
  • The Mobile Mapping System is an effective method to acquire the position and image data using vehicle equipped with the GPS (Global Positioning System), IMU (Inertial Measurement Unit), and CCD camera. It is used in various fields of road facility management, map update, and etc. In the general photogrammetry such as aerial photogrammetry, GCP (Ground Control Point)s are needed to compute the image exterior orientation elements (the position and attitude of camera). These points are measured by field survey at the time of data acquisition. But it costs much time and money. Moreover, it is not possible to make sufficient GCP as much as we want. However Mobile Mapping System is more efficient both in time and money because it can obtain the position and attitude of camera at the time of photographing. That is, Indirect Georeferencing must use GCP to compute the image exterior orientation elements, but on the other hand Direct Georeferencing can directly compute the image exterior orientation elements by GPS/INS. In this paper, we analyze about the positional accuracy comparison of ground point using the Direct Georeferencing and Indirect Georeferencing.

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차세대 전동차용 직접 구동용 매입형 영구자석 동기전동기의 특성 고찰 (Investigation on Direct Driven IPMSM for Next Generation Locomotive)

  • 김민석;박지성;김대광;김정철;정상용
    • 한국철도학회논문집
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    • 제11권4호
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    • pp.398-403
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    • 2008
  • 전동차용 견인전동기 시스템은 저항 및 쵸퍼 제어에 의한 직류 전동기 방식에서 인버터 제어에 의한 유도전동기 방식으로 발전하였다. 오늘날 유도전동기 기술은 어느 정도 성숙기에 접어들었지만 최근에는 소음제거, 효율 극대화 및 유지보수 비용 절감 등의 이점이 있는 감속기를 제거한 직접 구동용 영구자석 전동기가 새로이 각광받고 있다. 특히 기계적으로 견고한 매입형 영구자석 동기전동기는 출력/토크 밀도가 높고 약계자 제어성이 우수하며 고효율 운전이 가능하다. 본 논문은 자기적 포화특성이 심한 영구자석 전동기를 대상으로 고급화된 비선형 수치해석 기법을 적용하여 설계된 전동기의 특성해석을 나타낸다.

Development of Processing System of the Direct-broadcast Data from the Atmospheric Infrared Sounder (AIRS) on Aqua Satellite

  • Lee Jeongsoon;Kim Moongyu;Lee Chol;Yang Minsil;Park Jeonghyun;Park Jongseo
    • 대한원격탐사학회지
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    • 제21권5호
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    • pp.371-382
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    • 2005
  • We present a processing system for the Atmospheric Infrared Sounder (AIRS) sounding suite onboard Aqua satellite. With its unprecedented 2378 channels in IR bands, AIRS aims at achieving the sounding accuracy of radiosonde (1 K in 1-km layer for temperature and $10\%$ in 2-km layer for humidity). The core of the processor is the International MODIS/AIRS Processing Package (IMAPP) that performs the geometric and radiometric correction for generation of Level 1 brightness temperature and Level 2 geophysical parameters retrieval. The processor can produce automatically from received raw data to Level 2 geophysical parameters. As we process the direct-broadcast data almost for the first time among the AIRS direct-broadcast community, a special attention is paid to understand and verify the Level 2 products. This processor includes sub-systems, that is, the near real time validation system which made the comparison results with in-situ measurement data, and standard digital information system which carry out the data format conversion into GRIdded Binary II (GRIB II) standard format to promote active data communication between meteorological societies. This processing system is planned to encourage the application of geophysical parameters observed by AIRS to research the aqua cycle in the Korean peninsula.