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Screening method for amines by derivatization reaction on TLC (TLC 상 유도체화 반응을 이용한 아민 계 화합물의 Screening 방법)

  • Choi, Sung-Woon;Lee, Hye-In;Sung, Nack-Do
    • Analytical Science and Technology
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    • v.26 no.4
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    • pp.228-234
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    • 2013
  • Methamphetamine is an amine-containing illegal drug and is distributed unlawfully in South Korea. Finding a rapid, convenient and semi-quantitative determination method for methamphetamine is a very important issue in the area of forensic drug testing. As an effort to develop new screening method, the reactions between three organic compounds which are structurally similar to methamphetamine and N-(9-fluorenylmethoxycarbonyloxy) succinimide (FMOC-NHS) were performed on silica gel ($SiO_2$) TLC plates. Three reference compounds were synthesized and used for the identification, comparison and study of the limit of detection (LOD) of the products obtained from a direct reaction on a TLC plate. As a result, FMOC-NHS as a derivatization reagent generated compounds containing highly UV-active functional groups on the TLC plate after reacting with primary- and secondary amines. In the experiment 2D the LOD of amines was in the range of 0.045 and 0.01 mg/mL ($2{\mu}L/spot$), and in 1D the LOD was in the range of 0.002 and 0.007 mg/mL ($2{\mu}L/spot$). The LODs of the compounds tested were dependent on the concentration of the derivatizing reagent.

Art of National cultural in Chinese Animation (중국애니메이션에 나타난 민족문화예술성 연구)

  • Kim, Jin-Young;Kim, Jae-Woong
    • Cartoon and Animation Studies
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    • s.17
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    • pp.83-95
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    • 2009
  • As an exploratory research on China's animation, this study aims to enhance an understanding of the trends and characteristics of China's animation through examining its history and to forecast its future development trajectory. From its founding to recent period, China tried to maintain Communist political system through imbuing national identity to its people through management and supervision of media products under direct government's leadership in combination with ideological education. Such policy was also implemented in animation, major audience of which is children. With regard to the introduction of the policy and its influence, five historical phases could be identified as follows. During the first phase, from the founding of the Republic until the Cultural Revolution, national culture was introduced to China's animation. The second phase, which corresponds with the Cultural Revolution period, marks the decline of national culture. National culture was reemphasized during the third phase that follows the Cultural Revolution, which led to the nomination of the 'China school,' followed by the fourth phase, during which China's animation suffered the second decline due to the spread of TVs and foreign animation imports. Reintroduction of national culture on China's animation in the context of rapid industrialization process before and after 2000 characterizes the recent phase. It can be expected that although there could be some change in methods and forms, China's animation, which introduced national culture from its inception and maintained remarkable resilience following the period of decline, will continuously stress the its own national cultural identity.

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Process Design of Trimming to Improve the Sheared-Edge of the Vehicle Door Latch based on the FE Simulation and the Taguchi Method (유한요소해석 및 다구찌법을 이용한 자동차 도어 래치의 전단면 품질 향상을 위한 트리밍 공정 설계)

  • Lee, Jung-Hyun;Lee, Kyung-Hun;Lee, Seon-Bong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.11
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    • pp.483-490
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    • 2016
  • Automobile door latch is a fine design and assembly techniques are required in order to produce them in a small component assembly shape such as a spring, injection products, a small-sized motor. The door latch is fixed to not open the door of the car plays an important role it has a direct impact on the driver's safety. In this study, during trimming of the terminals of the connector main components of the car door latch, reduce rollover and conducted a research to find a suitable effective shear surface. Using the Taguchi method with orthogonal array of Finite Element Analysis and optimal Design of Experiments were set up parameters for the shear surface quality of the car door latch connector terminals. The design parameters used in the analysis is the clearance, the radius, and the blank holding force, the material of the connector terminal is a C2600. Trimming process optimum conditions suggested by the analysis has been verified by experiments, the shear surface shape and dimensions of a final product in good agreement with forming analysis results.Taguchi method from the above results in the optimization for the final rollover and effective shear surface improved for a vehicle door latch to the connector terminal can be seen that the applicable and useful for a variety of metal forming processes other than the trimming process is determined to be applicable.

A Study on Proposition of The Assisting Mechanism for Wheelchair Transfer for Car (차량용 휠체어 이송을 위한 보조메커니즘의 제안에 관한 연구)

  • Lim, K.;Kim, Y.S.;Yang, S.Y.
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.8 no.4
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    • pp.251-258
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    • 2014
  • A wheelchair is a typical mobility aid for the physically disabled or the old and the weak, and is the most commonly used rehabilitation aid. In general, most of users of manual wheelchair have difficulty though vocational rehabilitation and independent living is possible. The reason is that long-distance movement is always accompanied with wheelchair transfer problem because a wheelchair is used as direct means of transport. Hence, the wheelchair transfer problem should be first solved in order that a wheelchair user can independently live. Therefore, this study examined and analyzed the domestic and overseas launched products and patented technologies of wheelchair transfer system for vehicle, and proposed a wheelchair transfer mechanism of a new system for vehicle. This study proposed a wheelchair transfer mechanism for vehicle in order to remove the disadvantage of wheelchair transfer system for vehicle to support the conventional wheelchair user's movement, and in order to conform with the structure of domestic welfare vehicle for the disabled. Because a difference between storage space installed in the roof of vehicle and storage space for leisure, which is generally utilized, gets to disappear by applying this proposed mechanism, popularity among users can be increased. And storage space that has become smaller like this will be capable of decreasing the disadvantage of air resistance in traveling. Besides, because of getting to conform with the structure of welfare vehicle, restrictions on the application range will disappear from small sedan to SUV. Therefore, users can have more choices.

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Characteristics of Disinfection and Removal of 2-MIB Using Pulse UV Lamp (펄스 UV 램프를 이용한 미생물 소독 및 2-MIB 제거 특성)

  • Ahn, Young-Seog;Yang, Dong-Jin;Chae, Seon-Ha;Lim, Jae-Lim;Lee, Kyung-Hyuk
    • Journal of Korean Society of Water and Wastewater
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    • v.23 no.1
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    • pp.69-75
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    • 2009
  • The characteristics of disinfection and organic removal were investigated with pulse UV lamp in this study. The intensity and emission wavelength of pulse UV Lamp were compared with low pressure UV lamp. The emission spectrum range of pulse UV lamp was between 200 and 400 nm while the emission spectrum of low pressure UV lamp was only single wavelength of 254nm. 3 Log inactivation rate of B. subtilis spore by pulse UV and low pressure UV irradiation was determined as $44.71mJ/cm^2$ and $57.7mJ/cm^2$, respectively. This results implied that wide range of emission spectrum is more effective compared to single wavelength emission at 254nm. 500ng/L of initial 2-MIB concentration was investigated on the removal efficiency by UV only and $UV/H_2O_2$ process. The removal efficiency of UV only process achieved approximately 80% at $8,600mJ/cm^2$ dose. 2-MIB removal rate of $UV/H_2O_2$ (5 mg/L $H_2O_2$) process was 25 times increased compared to UV only process. DOC removal efficiency for the water treatment plant effluent was examined. The removal efficiency of DOC by UV and $UV/H_2O_2$ was no more than 20%. Removal efficiency of THMFP(Trihalomethane Formation Potential), one of the chlorination disinfection by-products, is determined on the UV irradiation and $UV/H_2O_2$ process. Maximum removal efficiency of THMFP was approximately 23%. This result indicates that more stable chemical structures of NOM(Natural Organic Matter) than low molecule compounds such as 2-MIB, hydrogen peroxide and other pollutants affect low removal efficiency for UV photolysis. Consequently, pulse UV lamp is more efficient compared to low pressure lamp in terms of disinfection due to it's broad wavelength emission of UV. Additional effect of pulse UV is to take place the reactions of both direct photolysis to remove micro organics and disinfection simultaneously. It is also expected that hydrogen peroxide enable to enhance the oxidation efficiency on the pulse UV irradiation due to formation of OH radical.

Prediction of chemical fertilizer consumption in relation to soil fertility improvement and various agriculturai technical factors (토양비옥도(土壤肥沃度) 증진(增進) 및 제(諸) 기술요인(技術要因)에 의(依)한 비료(肥料) 소비추세(消費趨勢) 전망(展望))

  • Ryu, In-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.9 no.3
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    • pp.183-199
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    • 1976
  • 1. The cultivated land in Korea has originally low fertility resulting in high dependence to fertilizers. 2. The total fertilizer requirement calculated by the Office of Rural Development (ORD) in 1976 was about 1,153 thousand mts, and the total amount of supply planned by the Ministry of Agriculture and Fishery was 1,010 thoushand mts which is close to the amount calculated by ORD. However, there is some gap between the amount of recommended (N, 11.6; $P_2O_5$: $K_2O$, 7.3kg/10a> and supply planned (N, 12.0; $P_2O_5$, 6.3; $K_2O$. 4.8kg/10a) fertilizers for each elements per unit area 3. For 15 years from 1960 to 1975 the fertilizer consumption of nitrogen was roughly increased from 200,000 mts to 500,000 mts; phosphorus, from 50,000 to 250,000 mts; potassium, from 10, 000 to 170,000 mts; accounting 2.5, 5, and 17 times of increase respectively. 4. The total fertilizer consumption for 5 years from 1967 to 1971 was about 100,000 mts and another 5years from 1971 to 1975 was 300,000 mts indicating three times increase. 5. The direct factors influenced to the increase of fertilizer consumption in recent years are 1) the dissemination of high yielding Tongil type rice varieties which are resistant to heavy fertilization 2) high price policy for agricaltural products 3) increased cultivation of vegetables:, fruits, and forages which require high level of fertilizers. The indirect factors are 1) dissemination of new improved agricultural techniques, 2, improvement of cultivated land conditions through irrigation system and land reform, 3) increased supply of silicate fertilizers, and 4) increase of farm income. 6. The percentage of total fertilizer consumption by rice (32%) and barley (25%) is about 57%. The ratio of total fertilizer consumption by vegetables and forages is expected to increase greatly. 7. Based on the increasing tendency of cultivated land and yield per unit area for last 10 years in each crop, total fertilizer consumptions in 1980, 1990, and 2000 year are estimated to 1,290,000, 1,580,000 and 1,870,000 mts respectively.

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Research on Reduction and Recycling of Food Waste by Separating Raw Food Waste and Earth Worm Composting in the Apartment (공동주택의 음식물 생쓰레기 분리배출과 지렁이퇴비화를 이용한 음식물쓰레기 감량 및 자원화 모델 연구)

  • Choi, Kwang-Soo
    • Journal of Environmental Science International
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    • v.20 no.1
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    • pp.137-146
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    • 2011
  • In this research, food waste source reduction model for apartment was investigated. In spite of prohibition of direct landfill of food waste and continuous efforts made by government and local government, food waste production increases steadily. Recycling ratio of the food waste increases every year, but its products have many problems like low quality, stability, salt, odor etc. Household occupies 63.3% of whole food waste production and this is subject to be a key factor to control food waste. We surveyed S apartment in Kwangju city as a model case, in which administrative office and women's association adopted clean plate eating, separation of raw food waste and earth worm composting as a series of method for source reduction and recycling inside the apartment. With the help of residents' participation and practice, food waste production decreased 15.6% from 0.31 g/capita/day in 2007 to 0.26 g/capita/day in 2009 (domestic average 0.30 g/capita/day). Separation of raw food waste and its composting using earth worm were very effective, and were subject to resolve the problems of present food waste treatment technologies. And earth worm composting was very useful in environmental, economical, societal and educational aspects. Instead of economical incentive, educational programs about food value, environmental problem and critical method for food waste separation were more effective for promotion of source reduction. From the analyses on the process and success factors in this model, we could conclude that leader's role was one of the key factors for the settlement of source reduction, and that was to understand the seriousness of the food waste and to seek solution, to test techniques, and to practice by oneself. Furthermore, networking and collaboration among residents, local government, NGO and local press promoted residents' participation, and it was through various education and investigation. Finally, source reduction and self recycling model of food waste in the apartment, that applies separating raw food waste and earth worm composting based on the collaboration among residents, local government, NGO, and local press, should be disseminated, and environmental policy also should be changed to make it possible.

The Effect of Ionizing Radiation on the Ultrastructural Changes and Mechanism on the Cytoplasmic Organelles (전리방사선이 세포질 소기관의 미세구조변화와 기전에 미치는 영향)

  • Lee, Moo Seok;Lee, Jong Kyu;Nam, Ji Ho;Ha, Tae Yeong;Lim, Yeong Hyeon;Kil, Sang Hyeong
    • Journal of Life Science
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    • v.27 no.6
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    • pp.708-725
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    • 2017
  • Ionizing radiation is enough energy to interact with matter to remove orbital electrons, neutrons, and protons in the atom. Ionizing radiation like this leads to oxidizing metabolism that alter molecular structure through direct and indirect interactions of radiation with the deoxyribonucleic acid in the nucleus and cytoplasmic organelles or via products of cytoplasm radiolysis. These ionization can result in tissue damage and disruption of cellular function at the molecular level. Consequently, ionizing radiation-induced modifications of ion channels and transporters have been reported. When the harmful effects exceed those of homeostatic biochemical processes, induced biological changes persist and may be propagated to progeny cells. Also, Reactive oxygen species formed on the effect of ionizing radiation can get across into neighboring cells through the cell junctions that are responsible for intercellular chemical communication, and may there bring about changes characteristic to radiation damage. Depending on radiation dose, dose-rate and quality, these protective mechanisms may or may not be sufficient to cope with the stress. This paper briefly reviewed reports on ionization radiation effects on cellular level that support the concept of radiation biology. A better understanding of the biological effects of ionizing radiation will lead to better use of and better protection from radiation.

The Catalytic Reduction of Carbon Dioxide by Butane over Nickel loaded Catalysts (니켈담지촉매상에서 부탄에 의한 이산화탄소의 환원반응)

  • Yoon, Cho-Hee;Kim, Geon-Joong
    • Applied Chemistry for Engineering
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    • v.8 no.3
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    • pp.543-549
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    • 1997
  • The direct reaction of carbon dioxide($CO_2$) with butane($C_4H_{10}$) to obtain synthesis gas and hydrocarbon compounds have been studied on nickel loaded catalysts. In the reaction of $CO_2$ with $C_4H_{10}$, Ni loaded catalysts showed similar activity with Pt catalyst and Coke deposition on the catalyst was severe by dehydrogenation of butane. The main products were carbon monoxide and hydrogen, when alumina and Y type zeolite were used as a support. Instead, a great deal of aromatic hydrocarbons were obtained on the Ni loaded ZSM-5 catalyst. The conversion of $CO_2$ increased with the increasing molar ratio of $CO_2$/$C_4H_{10}$ on Ni/ZSM-5, Ni/NaY and Ni/alumina catalyst, but the conversion decreased again from the ratio of 2. The value of $CO_2$ conversion was the highest at the 5wt% of Ni loading on ZSM-5 catalyst. A part of cokes deposited on the catalysts diminished when only $CO_2$ gas or water steam flowed into the reactor. The coke deposited on the catalysts was very reactive and it may be an important intermediate for the carbon dioxide reforming reaction.

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Hepatitis C Virus - Proteins, Diagnosis, Treatment and New Approaches for Vaccine Development

  • Keyvani, Hossein;Fazlalipour, Mehdi;Monavari, Seyed Hamid Reza;Mollaie, Hamid Reza
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.12
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    • pp.5917-5935
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    • 2012
  • Background: Hepatitis C virus (HCV) causes acute and chronic human hepatitis infection and as such is an important global health problem. The virus was discovered in the USA in 1989 and it is now known that three to four million people are infected every year, WHO estimating that 3 percent of the 7 billion people worldwide being chronically infected. Humans are the natural hosts of HCV and this virus can eventually lead to permanent liver damage and carcinoma. HCV is a member of the Flaviviridae family and Hepacivirus genus. The diameter of the virus is about 50-60 nm and the virion contains a single-stranded positive RNA approximately 10,000 nucleotides in length and consisting of one ORF which is encapsulated by an external lipid envelope and icosahedral capsid. HCV is a heterogeneous virus, classified into 6 genotypes and more than 50 subtypes. Because of the genome variability, nucleotide sequences of genotypes differ by approximately 31-34%, and by 20-23% among subtypes. Quasi-species of mixed virus populations provide a survival advantage for the virus to create multiple variant genomes and a high rate of generation of variants to allow rapid selection of mutants for new environmental conditions. Direct contact with infected blood and blood products, sexual relationships and availability of injectable drugs have had remarkable effects on HCV epidemiology. Hundreds of thousands of people die each year from hepatitis and liver cancer caused by HCV virus infection. Approximately 80% of patients with acute hepatitis C progress into a chronic disease state leading to serious hepatic disorders, 10-20% of which develop chronic liver cirrhosis and hepatocellular carcinoma. The incubation period of HCV is 6-8 weeks and the infection is often asymptomatic so it is very hard to detect at early stages, making early treatment very difficult. Therefore, hepatitis C is called a "silent disease". Neutralizing antibodies are produced against several HCV proteins during infection but the virus mutates to escape from antibodies. Some patients with chronic hepatitis C may have some symptoms such as fatigue, muscle aches, nausea and pain. Autoimmune and immunecomplex-mediated diseases have also been reported with chronic HCV infection.