• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.124.1-124.1
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• 2010

The impurities in the methanol fuel that is used for direct methanol fuel cell (DMFC) could greatly affect the performance of membrane electrode assemblies (MEA). The most common impurities in the commercial methanol fuel are mainly ethanol, acetone, acetaldehyde, or ammonia. In this study, the effect of impurities in methanol fuel was investigated on the performance of MEA. The MEA for DMFC were prepared using a semi-automatic bar-coating machine, which can prepare the catalyst layer with uniform thickness for MEA. As a result, a single cell supplied with one of the 6 different kinds of methanol fuels showed a significant degradation of the fuel cell performance. The most common impurities in the commercial methanol fuel is mainly ethanol, acetone, acetaldehyde, or ammonia. The effects of the kind and the concentration of impurities in the methanol fuels were investigated on the performance of MEA for DMFC. We will propose the optimum compositions and limit concentration of impurities in methanol fuel for high performance of MEA for DMFC.

• 한국자동차공학회논문집
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• 제20권4호
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• pp.39-45
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• 2012

Today gasoline engines for vehicular application are not only faced with stringent emission regulation but also with increasing requirements to better fuel economy, while guaranteeing power density. The spray-guided type gasoline direct injection (GDI) engine has an advantage of improved thermal efficiency and lower harmful emissions. Centrally mounted high pressure injector and adjacent spark plug allow stable lean combustion due to the flexible mixture stratification. In the present study, the performance and emissions characteristics of developed spray-guided type GDI combustion system were evaluated at various excess air ratio conditions. The specific fuel consumption and nitrogen oxides ($NO_x$) emissions were reduced due to the achievement of stable lean combustion under flammability limit. Multiple injection strategy was not helpful to improve fuel consumption while further reduction of $NO_x$ emissions was possible.

• 한국지진공학회논문집
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• 제22권5호
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• pp.299-309
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• 2018

Now that problems with force-based seismic design have been clearly identified, design is inclined toward displacement-based methods. One such widely used method is Direct-Displacement-Based Design (DDBD). Yet, one of the shortcomings of DDBD is considering higher-mode amplification of story shear, moments, and displacements using equations obtained from limited parametric studies of regular planar frames. In this paper, a different approach to account for higher-mode effects is proposed. This approach determines the lateral secant stiffness of the building frames that fulfill the allowable inter-story drift without exceeding the desired story displacements. Using the stiffness, an elastic response spectrum analysis is carried out to determine elastic higher-mode force effects. These force effects are then combined with DDBD-obtained first-mode force effects using the appropriate modal superposition method so that design can be performed. The proposed design procedure is verified using Nonlinear Time History Analysis (NTHA) of twelve planar frames in four categories accounting for mass and stiffness irregularity along the height. In general, the NTHA response outputs compared well with the allowable limits of the performance objective. Thus, it fulfills the aim of minimizing the use of NTHA for planar frame buildings, thereby saving computational resources and effort.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.99-102
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• 2003

The different methods such as HPLC, indirect- and direct-ELISA were employed for the analysis of microtoxins and the results of each method were compared in terms of the detection limit and accurary. Three toxins, microcystin-RR, -LR and -YR were clearly separated by HPLC using 0.05 M methanol and phosphate buffer used as a solvent system. The calibration curves for the toxins were linear in the range of 5 ng to 50 ng. The standard curves for the immunoassay of microcystin obtained by direct and indirect ELISA are compared. The linear responses of inhibitions of binding by microcystin in the direct and indirect competitive ELISA were in the range of 10 ng to 1000 ng and 50 pg to 160 pg, respectively. Distribution of microtoxins at 11 sites in the Nakdong river and several lakes in Korea was also studied. The most dominant microcystin variant in the test sites was found to be microcystin-RR.

• 한국소음진동공학회논문집
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• 제14권10호
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• pp.1007-1014
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• 2004

This paper presents a study of low frequencies volume velocity vibration control of a smart panel in order to reduce sound transmission. A distributed piezoelectric quadratically shaped polyvinylidene fluoride (PVDF) polymer film is used as a uniform force actuator and an array of $4\;{\times}\;4$ accelerometer is used as a volume velocity sensor for the implementation of a single-input single-output control system. The theoretical and experimental study of sensor-.actuator frequency response function shows that this sensor-actuator arrangement provides a required strictly positive real frequency response function below about 900 Hz. Direct velocity feedback could therefore be implemented with a limited gain which gives reductions of about 15 dB in vibration level and about 8 dB in acoustic power level at the (1,1) mode of the smart panel. It has been also shown that the shaping error of PVDF actuator could limit the stability and performance of the control system.

• 한국환경복원기술학회지
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• 제5권4호
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• pp.19-30
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• 2002

The stability of slope using root-pile like to the reinforcements is affected by the interaction behavior mechanism of soil-reinforcements. Through the studying on the interaction in joint of its, therefore, the control roles can be find out in installed slope. In study, the stress level ratio based on the insert angle of installed reinforcements in soil used to numerical analysis, which was results from the duty direct shear test in Lab. The maximum shear strain variation on the reinforcements was observed at insert angle, which was approximately similar to the calculated angle based on the equation proposed by the Jewell. The elasto-plastic joint model on the contact area of soil-reinforcements was presumed, the reinforced soil assumed non-linear elastic model and the reinforcements supposed elastic model, respectively. The finite element analysis of assumed models was performed. The shear strain variation of non-reinforced state obtained by the FEM analysis including elasto-plastic joint elements were shown the rationality of general limit equilibrium analysis for the slope failure mode on driving zone and resistance zone, which based on the stress level step according to failure ratio. Through the variation of shear strain for the variation of inserting angle of reinforcements, the different mechanism on the bending and the shear resistance of reinforcements was shown fair possibility.

• 농업과학연구
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• 제49권4호
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• pp.795-807
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1498-1504
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• 2005

Competitive direct ELISA was developed to detect gentamicin residues. Mice immunized with gentamicin-keyhole limpet hemocyanin (KLH) conjugate developed good antiserum titers, which gradually increased with booster injections, indicating immunization was successfully processed. Monoclonal antibody against gentamicin was prepared using hybridoma cells cloned by limit dilution of fused cells. IgG was purified from ascites fluid of hybridoma cell-injected mice through ammonium sulfate precipitation and Sephadex G-25 gel filtration. After the gel filtration, fractions of high antibody titer were further purified through affinity chromatography on protein A/G column. Monoclonal antibody against gentamicin was confirmed as IgG1, which has kappa light chain. Cross-reactivities ($CR_{50}$) of gentamicin monoclonal antibody to other aminoglycosides (kanamycin, neomycin, and streptomycin) were less than 0.005%, indicating the monoclonal antibody was highly specific for gentamicin. Standard curve constructed through competitive direct ELISA showed measurement range (from 80 to 20% of B/$B_0$ ratio) of gentamicin was between 1 and 40 ng/ml, and 50% of B/$B_0$ ratio was about 4 ng/ml. The gentamicin concentration rapidly increased to 1,300 ng/ml after the intramuscular administration up to 2 h, then sharply decreased to less than 300 ng/ml after 4 h of withdrawal, during which the elimination half-life ($t_{1/2}$) of gentamicin in the rabbit plasma was estimated to be 1.8 h. Competitive direct ELISA method developed in this study using the prepared monoclonal antibody is highly sensitive for gentamicin, and could be useful for detecting gentamicin residues in plasma of live animals.

• 무역학회지
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• 제46권3호
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• pp.135-149
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• 2021

This study analyzes the economic effect through the use of the RCEP direct transport rules, and suggests the necessacity of logistics efficiency and policy alternatives. The advantage of the hub network has been widely applied to the international logistics system, but there is a limit in the FTA logistics system in which goods must be directly transported between two contracting parties. Therefore, based on the new RCEP direct transport rules and the theoretical review on the possibility of an FTA logistics hub, FTA logistics efficiency improvement is estimated. This study quantitatively estimated the economic effect of direct transportation, unlike the previous studies, which were limited to the analysis of judicial precedents or surveys. GTAP model was used through five scenarios according to the impact of the RCEP tariff cut and the FTA logistics hub establishment in Singapore or Korea. As a result of the analysis, Korea's trade volume increased by 0.38% of exports and 1.63% of imports, and RCEP would increase exports by 0.27% and imports by 0.42%. In particular, the establishment of an FTA logistics hub (0.71%) was found to have a greater effect on the improvement of terms of trade than a tariff cut (0.12%), confirming the necessity of establishing an FTA logistics hub in RCEP. As a policy proposal, the institutional support of the customs authorities for the use of RCEP, the expansion of the free trade area where BWT traded cargo can be stored, and the establishment of a system for issuing back-to-back certificates of origin with approved exporters.

• 의료법학
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• 제21권3호
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• pp.3-33
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• 2020

'원격의료(telemedicine)'란 '의료인이 정보통신기술을 이용하여 원격으로 실시하는 의료행위'라고 정의할 수 있다. 지금까지 우리나라의 통설은 의료법 제34조를 근거로 의료인 간의 원격자문만 허용되고, 의료인과 환자 간의 원격의료는 금지되는 것으로 이해하였다. 그러나, 의료법 제34조는 의료업 수행에 대한 장소적 제한 규정일 뿐, 원격의료 자체를 금지하는 규정은 아니다. 그 외 현행 의료법에는 원격의료를 금지하는 규정이 존재하지 않는다. 건강보험 요양급여기준과 별개로 현행 의료법 해석상 원격의료가 일반적으로 금지된다고 보기는 어렵다. 다만, 의료법 제17조와 제17조의2에서의 '직접 진찰'의 의미와 관련해서 해석상 논란이 있다. 헌법재판소는 이를 '대면 진찰'로 해석한 반면, 대법원은 '스스로 진찰'로 해석하였다. '직접'의 사전적 의미와 관련 의료법 규정에 대한 해석 등에 비추어 볼 때, 대법원의 해석이 타당하다고 생각한다. '직접 진찰'이 '대면 진찰'을 의미하지는 않더라도, '진찰'의 개념 안에 '대면진찰의 원칙'이 내포되어 있고 '비대면 진찰'은 대면진찰을 보완하는 수준에서만 허용되기 때문에 비대면진찰로 인한 문제점은 충분히 극복할 수 있다고 본다. 결국은 진찰이 얼마나 충실하였느냐, 즉 '진찰의 충실성' 여부가 원격진료 허용의 한계라고 할 수 있다.